• 한국식품과학회지
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• 제44권4호
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• pp.493-497
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• 2012

약용식물 6종을 메탄올로 추출한 다음, n-hexane층을 DMSO에 녹여 항산화 활성을 측정 비교하여 기능성 소재인 천연 항산화제로써의 활용 가능성을 조사하였다. DPPH를 이용한 라디칼 소거능 실험에서는 250 ${\mu}g/mL$ 농도에서 보골지가 대조군인 ascorbic acid에 비해 높은 유리기 소거능에 의한 항산화 활성을 나타냈다. 농도 500 ${\mu}g/mL$에서는 ascorbic acid가 가장 높은 항산화 활성을 보였다. ABTS 유리기 소거능 실험에서는 농도 증가에 따른 항산화 활성이 DPPH assay와 상반된 결과를 나타냈고, 특히 보골지의 항산화 활성이 대조군인 ascorbic acid보다 유의적으로 높게 나타났다. DPPH 라디칼, ABTS 유리기 소거능 실험 결과 공통적으로 우수한 것으로 조사 된 약용 식물은 반지련, 보골지이며 500 ${\mu}g/mL$ 이상의 농도에서 ascorbic acid와 유사한 90% 활성을 보였으며 750 ${\mu}g/mL$ 농도에서는 ascorbic acid 보다 우수한 유리기 소거 활성을 보였다. 따라서, 반지련과 보골지는 항산화 활성이 우수하므로 천연 식물소재로 이용하기에 적합하며, 천연물의 추출 부위, 용매, 추출 조건에 따라 다양한 항산화 활성 능력을 기대 할 수 있으므로 천연 항산화제로서의 응용 가능성 또한 있을 것으로 사료된다.

• 한국식품영양과학회지
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• 제40권1호
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• pp.29-36
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• 2011

연구에서는 산채류 34종의 물 추출물과 메탄올 추출물을 제조한 후 항산화 활성을 탐색하여 산채류 유래 항산화물질을 탐색하고 기능성식품을 개발하기 위한 항산화능 우수 산채류 선발의 기초 자료를 제공하고자 하였다. 산채류시료 34종을 물과 메탄올 추출하였을 때 추출 수율은 물 추출의 경우 4.6~34.6%이었고, 메탄올 추출의 경우 3.4~45.0%이었다. 총 폴리페놀 함량은 물 추출물에서 4.6~183.8mg/g이고, 메탄올 추출물에서 8.2~270.4 mg/g이었다. 물과 메탄올 추출물에서 돌단풍(Aceriphyllum rossii )이 각각 183.8, 270.1 mg/g의 가장 높은 폴리페놀 함량을 보였고, 공통적으로 광대싸리(Securinega suffruticosa), 기린초(Sedum kamtschaticum), 까치수영(Lysimachia barystachys), 송이풀(Pedicularis resupinata)이 높은 폴리페놀 함량을 보였다. DPPH와 ABTS 라디칼 소거능은 까지수영, 돌단풍, 광대싸리, 기린초, 송이풀(Pedicularis resupinata), 승마(Cimicifuga heracleifolia), 짚신나물(Agrimonia pilosa), 뻐꾹채(Rhapontica uniflora)에서 우수하였다. 변수간의 상관성을 분석했을 때, 폴리페놀 함량과 DPPH 및 ABTS 라디칼 소거능, SOD 유사활성 사이에는 유의적인 양의 상관관계가 있었으며, 이들 측정치에서 공통적으로 우수한 것으로 조사된 산채류는 송이풀, 광대싸리, 뻐꾹채, 까치수영, 돌단풍이었다.

• 농업생명과학연구
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• 제44권3호
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• pp.69-78
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• 2010

핑크팝 보리수 열매와 잎을 기능성 식품의 재료로 활용하기 위하여 항산화 및 총 페놀성 화합물 함량을 측정한 결과는 다음과 같다. DPPH 및 ABTS radical 소거활성을 측정한 결과 분획물의 농도가 증가함에 따라 DPPH 및 ABTS radical 소거활성이 증가하는 경향을 보였으며, 특히 잎의 부탄올 분획물에서 가장 높은 DPPH radical 소거활성을 보였다. 또한 ABTS 라디칼 소거활성도 열매와 잎의 여러 분획물 중 잎의 부탄올 분획물에서 가장 높은 소거활성을 보였다. 환원력과 FRAP 활성도 분획물의 농도가 증가함에 따라 환원력과 FRAP 활성이 증가하는 경향을 보였으며, 잎의 부탄올 분획물에서 가장 높은 환원력과 FRAP 활성을 보였다. Linoleic acid를 이용한 자동산화 억제활성을 실험한 결과 다른 분획물에 비하여 부탄올 분획물에서 가장 높은 과산화 억제활성을 나타내었으며, 총 페놀성 화합물은 잎과 열매의 부탄올 분획물에서 각각 106.34 및 252.46 mg/g으로 가장 높은 함량을 보였다. 핑크팝 보리수 잎 부탄올 분획물의 항산화 활성은 페놀성 화합물에 의한 것이며, 이는 자유 라디칼에 의해 유발된 산화적 스트레스를 효과적으로 방어할 수 있을 것이다.

• Preventive Nutrition and Food Science
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• 제14권4호
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• pp.283-289
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• 2009

We evaluated total phenolics and antioxidative activities of water extracts from different parts of Cudrania tricuspidata (specifically, the leaves, stems, roots, and fruits). The antioxidative activities of these samples were determined using five methods, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical scavenging, reducing power, ferric reducing ability of plasma (FRAP), and $\beta$-carotene/linoleic acid system. The water extract of leaves exhibited the higher DPPH, ABTS radical scavenging activities, reducing power, and FRAP than water extract of stem, roots, and fruits. Inhibition values on linoleic oxidation of water extracts from leaves, stems, roots, and fruits were calculated as 45.98%, 33.03%, 39.73%, and 25.48% at 10 mg/mL, respectively. The water extract of C. tricuspidata leaves had the highest amount of toal phenolics (73.60$\pm$0.28 mg/g). High-performance liquid chromatography (HPLC) analysis showed that quercetin is the predominant phenolic compound in water extract of leaves. Thus, our study verified that the water extract of leaves has strong antioxidant activities which are correlated with its high level of phenolic compounds, particularly quercetin. This water extract of C. tricuspidata leaves can be used as an effective and safe source of antioxidants.

• 한국조리학회지
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• 제22권3호
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• pp.108-120
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• 2016

본 연구에서는 복숭아 분말의 첨가량을 달리하여 젤리를 제조한 후 젤리의 물리화학적 품질특성(pH, 색도, 외관색상, 경도)과 소비자 기호도(색, 향미, 씹힘성, 맛, 전체적 기호도)를 비교 분석하였다. 젤리의 pH는 증가하였다(p<0.001). 명도를 나타내는 $L^*$값은 복숭아 분말의 첨가량이 증가할수록 유의적으로 감소하였고(p<0.001), 적색도를 나타내는 $a^*$값과 황색도를 나타내는 $b^*$값은 유의적으로 증가하였다(p<0.001). 복숭아 분말 첨가 젤리의 DPPH 라디칼 소거능에 대한 결과는 복숭아 분말 첨가량이 많을수록 DPPH 라디칼 소거능이 증가하였다. 소비자 검사결과, 15% 첨가군이 모든 평가항목에서 유의적으로 높은 평가를 받아 젤리의 관능적 품질을 최대한 유지하면서 복숭아 분말의 기능적 이점을 최대한 활용하기 위한 젤리의 최적첨가 농도는 15%가 가장 적합한 것으로 판단된다.

• 대한약침학회지
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• 제10권3호
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• pp.53-62
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• 2007

Objective The objective of this study was to investigate the antioxidative effects of Puerariae Radix extract. Method Total antioxidant capacity (TAC), Total antioxidant response (TAR), Total phenolic content, Reactive oxygen species (ROS), 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activities, lipid peroxidation were examined. Result Total antioxidant status was examined by total antioxidant capacity(TAC) and total antioxidant response(TAR) against potent free radical reactions. TAC and TAR of Puerariae Radix extract at the concentration of 5 mg/ml were 2.02 and 1.50 mM Trolox equivalents, respectively. Total phenolic content of Puerariae Radix extract at the concentration of 5 mg/ml was 2.29 mM gallic acid equivalent. Concentration of Puerariae Radix extract at which DPPH radical scavenging activity was inhibited by 50% was 5.91 mg/ml as compared to 100% by pyrogallol solution as a reference. The inhibitory effect of the extract on lipid peroxidation was examined using rat liver mitochondria induced by FeSO4/ascorbic acid. Puerariae Radix extract at the concentration of 1 mg/ml slightly but significantly decreased TBARS concentration. The extract further prevented lipid peroxidation in a dose-dependent manner. The effect of Puerariae Radix extract on reactive oxygen species (ROS) generation was examined using cell-free system induced by hydrogen peroxide/FeSO4. Addition of 1 mg/ml of Puerariae Radix extract significantly reduced dichloroflurescein (DCF) fluorescence. The extract caused concentration-dependent attenuation of the increase in DCF fluorescence, indicating that the extract significantly prevented ROS generation in vitro. Thus antioxidant effects of Puerariae Radix extract seem to be due to, at least in part, the prevention from free radicals-induced oxidation, followed by inhibition of lipid peroxidation. Conclusion As a result, Puerariae Radix seems to have antioxitative effect and antioxidant compount.

• 센서학회지
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• 제29권5호
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• pp.293-302
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• 2020

Food safety has emerged as a growing concern for human health in recent times. Consuming contaminated food may lead to serious health problems, and therefore, a system for monitoring food freshness that is both non-detrimental to the quality of food and highly accurate is required to ensure that only high-quality fresh food packages are provided to the customers. This paper proposes a method to monitor and detect food quality using a compact smart sensor tag. The smart tag is composed of three ultra-low-power sensors, which monitor four major indicators of food freshness: temperature, humidity, and the concentrations of ammonia and hydrogen sulfide gases. An RF energy scavenging circuit is integrated into the smart sensor tag to harvest energy from radio waves at a high frequency of 13.56 MHz to supply sufficient power to the tag. Experimental results show that the proposed energy harvester can efficiently obtain energy at a distance of approximately 40 cm from a 4 W reader. In addition, the proposed smart sensor tag can operate without any battery, thereby eliminating the requirement of frequent battery replacement and consequently decreasing the cost. Meanwhile, the freshness of preserved pork is continuously monitored under two conditions--room temperature and refrigerator temperature--both of which are the most common temperatures under which food is generally stored. The food-monitoring experiments are conducted over a period of one week using the proposed battery-less tag. Based on the experimental results, the food assessment is classified into four categories: fresh, normal, low, and spoiled.

• 대한화장품학회지
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• 제32권3호
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• pp.129-134
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• 2006

본 연구는 곰취 추출물의 항산화, 지질과산화 억제 및 자외선 조사에 의해 유도된 MMP-1 발현에 대한 영향을 사람 섬유아세포를 이용하여 확인하였다 곰취의 DPPH와 superoxide radical 소거효과는 처리농도가 증가함에 따라 농도 의존적으로 소거효과를 나타냈으며, 각각 5mg/mL에서 82.3%와 79.3%로 DPPH와 superoxide radical을 소거하여 우수한 항산화 효과를 나타내었다. 곰취 추출물의 지질과산화 억제효과는 $500{\mu}g/mL$에서 97.0%로 지질과산화 억제효과도 우수하게 나타났다. 또한 자외선에 의해 증가된 사람 섬유아세포의 MMP-1 단백질 발현양은 곰취 추출물을 $100{\mu}g/mL$ 농도를 처리함으로써 약 35%로 감소되었다. 곰취 추출물은 항산화 효과, 지질과산화 억제효과 및 자외선 조사에 의해 유도되는 MMP-1 단백질 발현량을 조절하는 기능을 갖는 것을 확인하였다. 따라서 곰취는 항산화, 지질과산화 억제 및 자외선에 의해 유도되는 MMP-1 발현을 저해함으로써 항노화 소재로써 이용될 수 있을 것으로 사료된다.

• Journal of Acupuncture Research
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• 제25권1호
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• pp.57-72
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• 2008

Objectives : The Herb-combined Remedy(HCR) for diabetes mellitus is known as an anti-hyperglycaemic agent. But its exact mechanisms are unclear. The present study was carried out to investigate its anti-hyperglycaemic and anti-oxidative effects in STZ-induced diabetic rats. Methods : Experimental diabetes was induced by injection of STZ(80mg/kg) to ratsvia the peritoneum. The experimental animals were divided into 4 groups : normal group, control group(STZ-induced diabetic rats with no treatment), HCR group(STZ-induced diabetic rats with HCR treatment), MF group(STZ-induced diabetic rats with Metformin treatment). The effects of HCR on STZ-induced diabetes was observed by measuring fasting blood glucose, changes of body weight, food uptake, and water uptake glucose levels in the normal state decline rates in blood glucose levels DPPH free-radical scavenging activity superoxide dismutase in RBC lysate catalase activity in RBC lysate and glutathione reductase activity in RBC lysate. Results : Treatment with HCR regulated blood glucose levels. Treatment with HCR also prevented weight loss in STZ-induced diabetic rats. In addition, oral glucose tolerance decreased following treatment with HCR. Direct anti-oxidative effects on DPPH free-radical scavenging were not observed, but treatment with HCR elevated SOD levels in blood cell lysates from STZ-induced diabetic rats. In addition, the HCR-treatment group showed an elevated tendency to glutathione reductase activity. Conclusions : These results demonstrate that HCR has anti-hyperglycaemic and anti-oxidative effects in STZ-induced diabetic rats.

• Preventive Nutrition and Food Science
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• 제18권1호
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• pp.18-22
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• 2013

To investigate radical scavenging effects and protective activities of bitter melon (Momordica charantia) against oxidative stress, in vitro and a cellular system using LLC-$PK_1$ renal epithelial cells were used in this study. The butanol (BuOH) fraction of bitter melon scavenged 63.4% and 87.1% of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals at concentrations of 250 and $500{\mu}g/mL$, respectively. In addition, the BuOH fraction of bitter melon effectively scavenged hydroxyl radicals (${\cdot}OH$). At all concentrations tested, the scavenging activity of the BuOH fraction was more potent than that of the positive control, ascorbic acid. Furthermore, under the LLC-$PK_1$ cellular model, the cells showed a decline in viability and an increase in lipid peroxidation through oxidative stress induced by pyrogallol, a generator of superoxide anion ($O_2{^-}$). However, the BuOH fraction of bitter melon significantly and dose-dependently inhibited cytotoxicity. In addition, 3-morpholinosydnonimine (SIN-1), a generator of peroxynitrite ($ONOO^-$) formed by simultaneous releases of nitric oxide and $O_2{^-}$, caused cytotoxicity in the LLC-$PK_1$ cells while the BuOH fraction of bitter melon ameliorated oxidative damage induced by $ONOO^-$. These results indicate that BuOH fraction of bitter melon has protective activities against oxidative damage induced by free radicals.