• Title/Summary/Keyword: Scanning Electron Microscope& #40;SEM& #41;

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Novel Encapsulation with New Glyceryl Ester Vesicle Enhances Stability of Nanoemulsion Containing Astaxanthin (아스타잔틴을 포함하는 나노에멀젼의 안정성 향상을 위한 신규 Glyceryl Ester 이용 캡슐화)

  • Kim, Dong Myung;Hong, Weon Ki;Kong, Soo Sung;Lee, Un Yep
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.39 no.3
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    • pp.225-231
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    • 2013
  • Oil in water nanoemulsion of astaxanthin was prepared by high pressure homogenization. The emulsifying conditions including emulsifier type, concentration and astaxanthin concentration were optimized. Stability of nanoemulsion was measured using zetasizer, freeze-fracture scanning electron microscope (FF-SEM), particle analyzer and colorimeter. The mean diameter of the dispersed particles containing astaxanthin ranged from 160 to 190 nm. Size distribution was unimodal and extended from 40 to 200 nm. The nanoemulsion prepared by glyceryl citrate/lactate/linoleate/oleate had smaller particle size and narrow size distribution. Stable incorporation of astaxanthin in nanoemulsion was performed and checked using high performance liquid chromatography (HPLC), freeze-fracture scanning electron microscope (FF-SEM). Physical stability of nanoemulsion was not significantly changed during storage at both light and thermal condition for a month with zeta potential value of -41 mV meaning stable colloid.

Larval Gnathostoma hispidum detected in the red banded odd-tooth snake, Dinodon rufozonatum rufozonatum, from China

  • Cho, Shin-Hyeong;Kim, Tong-Soo;Kong, Yoon;Na, Byoung-Kuk;Sohn, Woon-Mok
    • Parasites, Hosts and Diseases
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    • v.45 no.3
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    • pp.191-198
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    • 2007
  • A total of 205 larval gnathostomes were collected from 18 (22.5%) of 80 red banded odd-tooth snakes, Dinodon rufozonatum rufozonatum, which had been smuggled from China and confiscated at Customs in Susan, Republic of Korea. In order to identify the species, some of the larvae were observed by a light microscope and a scanning electron microscope (SEM). The larvae were $2.18{\times}0.29mm$ in average size, and had a pair of lips at the anterior end, a muscular esophagus, 2 pairs of cervical sacs, and brownish intestines. The head bulb was characteristically equipped with 4 rows of hooklets; the average number of hooklets in each respective row was 38.6, 40.5, 41.5, and 43.7. In SEM views, the mouth evidenced a pair of lateral lips of equal size in a half-moon shape. Each lip featured a couple of labial papillae and a small amphid located between the 2 papillae. The hooklets on the head bulb had single-pointed, posteriorly-curved tips. The cuticular spines were larger and more densely distributed on the anterior part of the body, and decreased gradually in size and number toward the posterior body. On the basis of these morphological characteristics, the larvae were identified as the third stage larvae of Gnathostoma hispidum.

Effect of Liquefied Digestive Medicine on the Surface of Composite Resin

  • Kim, Min-Young;Lim, Hee-Jung;Kim, Ha-Eun;Kim, Hyun-Jeong;Yu, Hye-Kang;Choi, Soo-Jin;Lim, Do-Seon
    • Journal of dental hygiene science
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    • v.22 no.1
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    • pp.20-29
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    • 2022
  • Background: The purpose of this study is to investigate the effect of liquefied digestive medicines on the composite resin surface. Methods: Three types of liquefied digestive medicines (Gashwalmyeongsu, Wicheongsu, and Saengrokcheon) were selected as experimental groups, Samdasoo and Chamisul as negative controls, and Trevi as positive controls were selected to measure pH and titratable acidity. The samples filled with resin at acrylic were made total 300, 50 per group. To evaluate the erosion risk of the composite resin, the specimens were immersed in a liquefied medicine for 1, 3, 5, 15, and 30 minutes, and then the surface microhardness was measured using the Vickers Hardness Number, and the surface change was observed with scanning electron microscope (SEM). Results: The average pH of the three liquefied medicine was 3.75±0.30, the Saengrokcheon was the lowest at 3.45±0.01, and the Trevi was 4.66 and Samdasoo and Chamisul were 7.40 and 8.58, respectively. The amount of NaOH reaching pH 5.5 and 7.0 was the lowest in the order of Trevi, Gashwalmyeongsu, Wicheongsu, and Saengrokcheon. The largest surface hardness reduction value was shown in Gashwalmyeongsu (-11.85±3.73), followed by Saengrokcheon (-9.79±3.11) and Wicheongsu (-8.28±2.83), and Samdasoo (-0.84±1.56) and Chamisul (-6.24±0.42) had relatively low surface hardness reduction values. However, Trevi (-16.67±5.41), a positive control group containing carbonic acid, showed a higher decrease in surface hardness than the experimental group. As a result of observation with SEM, experimental group and positive control group, showed rough surfaces and irregular cracks, and negative control groups showed smooth patterns similar to before immersion. Conclusion: The liquefied digestive medicine with low pH could weaken the composite resin surface, and the carbonic acid component could more effect on the physical properties of the composite resin than pH.

AN EXPERIMENTAL STUDY ON THE DIMENSIONAL CHANGES OF RELINED DENTURES AND MECHANICAL PROPERTIES AND SURFACE TEXTURES OF SEVERAL RESINS USED IN DENTURE RELINING (수종 레진으로 의치상 조직면 개조시 의치상의 크기변화와 물리적 성질 및 표면상태 비교 연구)

  • Lee, Chang-Han;Kim, Yung-Soo
    • The Journal of Korean Academy of Prosthodontics
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    • v.28 no.1
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    • pp.25-41
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    • 1990
  • The purpose of this study was to evaluate and compare the dimensional changes of relined dentures with a light-curing resin, a heat-curing resin, and a direct, hard reline resin. And also to measure the transverse strength, impact strength, surface hardness of the three resins used in relining. The surface textures of three resins also of evaluated by using scanning electron microscope. Through analyses on the data from this study, the following conclusions were obtained. 1. Impact strength of heat-curing resin was highest, and direct, hard reline resin higher, light-curing resin lowest. 2. Transverse strength of heat-curing resin was highest, and direct, hard reline resin and light-curing resin was lower and not signiicantly different. 3. Surface hardness of light-curing resin was lighest, heat-curing resin higher, and direct, hard reline resin was lowest. 4. After storage of the relined dentures for 1 day and 1 week in water at room temperature, linear shrinkage of distance between the reference points in the maxillary base relined with direct, hard reline resin was lowest, and those relined with light-curing resin and heat-curing resin were lower and were not significantly different. 5. After storage for 4 weeks in orator at room tempeature, linear shrinkage of distance between ridge crests of dentures relined with heat-curing resin was highest and that of distance between denture borders was not significantly different. 6. The dimensional changes of relined dentures during storage in water was not significant except those of distance between denture borders relined with light-curing resin at 1 day and 1 week storage in water. 7. At low magnification (x40) of SEM examination, the surface textures of three resins were similar except light-curing resin which had some defects. At high magnification (x200), the surface textures of hard, direct reline resin were smooth with little defects, but those of heat-curing resin and light-curing resin w ere irregular.

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3D quantitative analysis and SEM qualitative analysis of natural antagonist enamel opposing CAD-CAM monolithic zirconia or lithium disilicate tooth-supported crowns versus enamel opposing natural enamel

  • Piyarat Woraganjanaboon;Chuchai Anunmana
    • The Journal of Advanced Prosthodontics
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    • v.16 no.1
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    • pp.12-24
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    • 2024
  • PURPOSE. This study aimed to evaluate the maximum vertical wear, volume wear, and surface characteristic of antagonist enamel, opposing monolithic zirconia or lithium disilicate crowns. MATERIALS AND METHODS. The study comprised 24 participants (n = 12), who were randomly allocated to receive either a 5 mol% Y-TZP or a lithium disilicate crown in positions which would oppose the natural first molar tooth. The contralateral first molar along with its antagonist was considered as the enamel opposing natural enamel control. Data collection was performed using an intraoral scanner and polyvinylsiloxane impression. The means of the maximum vertical loss and the volume loss at the occlusal contact areas of the crowns and the various natural antagonists were measured by 3D comparison software. A scanning electron microscope was subsequently used to assess the wear characteristics. RESULTS. The one-year results from 22 participants (n = 11) indicated no significant differences when comparing the zirconia crown's antagonist enamel (40.28 ± 9.11 ㎛, 0.04 ± 0.02 mm3) and the natural enamel wear (38.91 ± 7.09 ㎛, 0.04 ± 0.02 mm3) (P > .05). Also, there is no significant differences between lithium disilicate crown's antagonist enamel (47.81 ± 9.41 ㎛, 0.04 ± 0.02 mm3) and the natural enamel wear (39.11 ± 7.90 ㎛, 0.04 ± 0.02 mm3) (P > .05). CONCLUSION. While some studies suggested that monolithic zirconia caused less wear on opposing enamel than lithium disilicate, this study found similar wear levels to enamel for both materials compared to natural teeth.

AN EXPERIMENTAL STUDY OF NEWLY DESIGNED IMPLANT WITH RBM SURFACE IN THE RABBIT TIBIA : RESONANCE FREQUENCY ANALYSIS AND REMOVAL TORQUE STUDY

  • Won Mi-Kyoung;Park Chan-Jin;Chang Kyoung-Soo;Kim Chang-Whe;Kim Yung-Soo;Isa Zakiahbt Mohd;Ariffin Yusnidar Tajul
    • The Journal of Korean Academy of Prosthodontics
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    • v.41 no.6
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    • pp.720-731
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    • 2003
  • Statement of problem. The importance of fixture design and surface treatment. Purpose. The clinical success of dental in plants is affected by many factors such like as degree of osseointegration, the effective load dispersion for the prostheses, and a lot of attempts have been made to overcome the difficulties. In this study, efforts were made to find the possibility of clinical acceptance of the dental implants of newly designed surface and resorbable blast media surcace. Materials and methods. In this study, two groups of custom-made, screw-shaped implants were prepared. The first with the consisting of Branemark clone design and the other with the new design. These implants were divided into four groups according to the kinds of surface treatment. Four implants($AVANA^{(R)}$, Osstem, Busan, Korea)of each group were installed in twenty rabbits. Group A was consisted of Branemark done implant left as machined, Group B with Branemark clone implants with RBM(Resorbable blast media) surface, Group C with newly designed implants left as machined and Group D with newly designed implants with RBM surface. One of the twenty rabbits died from inflammation and the observation was made for six weeks. Specimens from four groups were observed using scanning electron microscopy with 40, 100, 1000 magnification power and microsurface structures were measured by white-light scanning interferometry for three dimensional surface roughness measurements(Accura $2000^{(R)}$, Intek-Plus, Korea.). Removal torque was measured in 17 rabbits using digital torque gauge(MGT 12R, Mark-10 corp., NY, U.S.A.) immediately after the sacrifice and two rabbits were used for the histologic preparation(EXAKT $310^{(R)}$, Heraeus Kulzer, wehrheim, Germany) of specimens and observed under light microscope. Resonance frequency measurement($Osstell^{(R)}$) was taken with the 19 rabbits at the beginning of the implant fixation and immediately after the sacrifice. Results. Following results were taken from the experiment. 1. The surface of the RBM implants as seen with SEM had rough and irregular pattern with reticular formation compared to that of fumed specimens showing different surface topographies. 2. The newly designed implant with RBM surface had high removal torque value among four groups with no statistical significance. The average removal torque was $49.95{\pm}6.70Ncm$ in Group A, $51.15{\pm}4.40Ncm$ in Group B, $50.78{\pm}9.37Ncm$ in Group C, $51.09{\pm}4.69Ncm$ in Group D. 3. The RFA values were $70.8{\pm}4.3Hz$ in Group A, $71.8{\pm}3.1Hz$ in Group B, $70.9{\pm}2.5Hz$, $72.7{\pm}2.5Hz$ in Group D. Higher values were noted in the groups which had surface treatment compared to the untreated groups with no statistical significance. 4. The results from the histomorphometric evaluation showed a mean percentage of bone-to-implant contact of $45{\pm}0.5%$ in Group A, $55{\pm}3%$ in Group B, $49.5{\pm}0.5%$ in Group C, and $55{\pm}3%$ in Group D. Quite amount of newly formed bone were observed at the surface RBM-treated implants in bone marrow space.