• Korean Journal of Human Ecology
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• v.21 no.3
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• pp.539-550
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• 2012

In-depth knowledge of fabric microstructure is essential for understanding clothing comfort since it plays a significant role in heat and mass transfer between the human body and clothing. In this study, a novel method was employed for investigating 3D surfaces and solid construction characteristics of specific fabrics by using a reverse engineering technique. The surface construction data were obtained by a confocal laser scanning microscope and then manipulated by a 3D analysis program. Triangle mesh was used for connecting each 3D point, with clouds and fabric surface characteristics created by rendering techniques. For generating a 3D solid model, determinants of radius of curvature was used. According to the proposed method, actual surface expression of the real fabric was achieved successfully. The results from this methodology can be applied to the detailed analysis of clothing comfort that is highly influenced by the microstructure of the fabric.

• Journal of Microbiology and Biotechnology
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• v.11 no.3
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• pp.458-462
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• 2001

The localization of paclitaxel was investigated in suspension culture cells of Taxus chinensis. Over 93% of the cell-associated paclitaxel were detected throughout the entire culture period. Intracellular localization of paclitaxel over the culture time was analyzed further by cell fractionation for days 21 and 42. Paclitaxel contents in intracellular organelles were decreased at day 42, while the content in the cell wall fraction was increased at day 42 compared to the value for day 21. The localization of paclitaxel in the cell wall was confirmed by using the immunocytochemical method with the aid of a confocal laser scanning microscope.

• Journal of Technologic Dentistry
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• v.35 no.4
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• pp.281-286
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• 2013

Purpose: Surface modification is important techniques in modern dental and orthopedic implants. This study was performed to try embedding of bioactive materials in porous Ti implants. Methods: Porous Ti implant samples were fabricated by sintering of spherical Ti powders in a high vacuum furnace. It's diameter and height were 4mm and 20mm. Embedding process was used to suction and vacuum chamber. Loading properties of porous Ti implants were evaluated by scanning electron microscope(SEM), confocal laser scanning microscope(CLSM), and UV-Vis-NIR spectrophotometer. Results: Internal pore structure was formed fully open pore. Average pore size and porosity were $10.253{\mu}m$ and 17.506%. Conclusion: Porous Ti implant was fabricated successfully by sintering method. Particles are necking strongly each other and others portions were vacancy. This porous structure can be embedded to bioactive materials. Therefore bioactive materials will be able to embedding to porous Ti implants. Bioactive materials embedding in the porous Ti implant will induced new bone faster.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.06a
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• pp.577-580
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• 2005

Surface quality of a micro-processed sample with laser has been investigated by using of scanning confocal microscope(SCM) and atomic force microscope(AFM). Samples are bump electrodes and ITO glass of LCD module used in a mobile phone and a wafer surface scribed by UV laser. A image of $140\times120{\mu}m^2$ is obtained within 1 second by SCM because scan speed of a x-axis and y-axis are 1kHz and 1Hz, respectively. AFM is able to measure correctly hight and width of ITO and scribing depth and width of a wafer with a resolution less than 300 . However, the scan speed is slow and it is difficult to distinguish a surface composed of different nm kinds of materials. Results show that SCM is preferable to obtain a image of a sample composed of different kinds of material than AFM because the intensity of a reflected light from surface is different from each material.

• Journal of the Korean Society for Precision Engineering
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• v.23 no.4 s.181
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• pp.52-59
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• 2006

Surface qualities of a micro-processed sample with a pulse laser have been investigated by making use of scanning confocal microscope(SCM) and atomic force microscope(AFM). Samples are bump electrodes and ITO glass of LCD module used in a mobile phone and a wafer surface scribed by UV laser. A image of $140{\times}120{\mu}m^2$ is obtained within 1 second by SCM because scan speed of a x-axis and y-axis are 1kHz and 1Hz, respectively. AFM is able to correctly measure the hight and width of ITO, and scribing depth and width of a wafer with a resolution less than 300nm. However, the scan speed is slow and it is difficult to distinguish a surface composed of different kinds of materials. Results show that SCM is preferable to obtain a image of a sample composed of different kinds of material than AFM because the intensity of a reflected light from the surface is different for each material.

• Journal of Soil and Groundwater Environment
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• v.8 no.4
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• pp.1-11
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• 2003

In order to measure aperture variation dependent on normal stress and to characterize on relationship between aperture variation and hydraulic conductivity this study measured apertures of rock fractures under a high resolution confocal laser scanning microscope (CLSM) with application of five stages of uniaxial normal stresses. From this method the response of aperture can be continuously characterized on one specimen by different loads of normal stress. The results of measurements showed a rough geometry of fracture bearing non-uniform aperture. They also revealed different values of aperture variations according to the load stages on each position along a fracture due to the fracture roughness. Laboratory permeability tests were also conducted to evaluate the changes of permeability coefficients related to the aperture variations by different loads. The results of permeability tests revealed that the hydraulic conductivity was not reduced at a fixed rate with increase of normal load. Moreover, the rates of aperture variations did not match to those of hydraulic conductivity. The hydraulic conductivity calculated in this study did not follow the cubic law, representing that the parallel plate model is not suitable to express the fracture geometry corresponding to the results of aperture measurements under the CLSM.

• Tribology and Lubricants
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• v.24 no.6
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• pp.320-325
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• 2008

The recent industrial application requires technical methods to get the cutting fluid droplet surfaces in particular from the viewpoint of topography and micro texture. To characterize the surface topography of droplet, the combination of the confocal laser scanning microscope (CLSM) and wavelet filtering is well suited for obtaining the droplet geometry encountered in tribological research. This technique indicates a better agreement in obtaining an appropriate droplet surface obtained by the CLSM over a detail range of surface accuracy (resolution: $2{\mu}m$). And the results allow an excellent accuracy in a measurement of a droplet surface. The combination of extended focal depth measurement configured and multi-scale wavelet filtering has proven that it can construct a droplet surface in a successive and accurate way. A multi-scale approach of wavelet filtering was developed based on the decomposition and reconstruction of droplet surface by 2D wavelet transform using db9 (a mother wavelet of daubechies). Also this technique can be extended to characterize the quantification of droplet properties and other field in a wide range of scales. Finally this method is verified to be a better droplet surface modeling in a micro scale arising in a mist machining.

• Restorative Dentistry and Endodontics
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• v.40 no.2
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• pp.143-148
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• 2015

Objectives: This study evaluated the maximum depth and percentage of irrigant penetration into dentinal tubules by passive ultrasonic irrigation (PUI). Materials and Methods: Thirty extracted human teeth were instrumented and divided into three groups. According to final irrigation regimen, 5.25% sodium hypochlorite (Group A, NaOCl), 2% chlorhexidine (Group B, CHX) and saline solution (Group C, control group) were applied with Irrisafe 20 tips (Acteon) and PUI. Irrigant was mixed with 0.1% rhodamine B. Sections at 2 mm, 5 mm, and 8 mm from the apex were examined with confocal laser scanning microscopy (CLSM). The percentage and maximum depth of irrigant penetration were measured. Kruskal-Wallis test and Mann-Whitney test were performed for overall comparison between groups at each level and for pairwise comparison, respectively. Within a group, Wilcoxon test was performed among different levels. p values less than 0.05 were considered significant. Results: In all groups, highest penetration depth and percentage of penetration were observed at the 8 mm level. At 2 mm level, Groups A and B had significantly greater depths and percentages in penetration than Group C (p < 0.05), but there were no significant differences between Groups A and B. At 5 mm level, penetration depths and percentage of penetration was not significantly different among the groups. Conclusions: NaOCl and CHX applied by PUI showed similar depth and percentage of penetration at all evaluated levels.

• Journal of the Korean Society for Heat Treatment
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• v.24 no.5
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• pp.262-270
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• 2011

Carbide precipitation and dissolution behavior at various temperatures during heat treatment has been studied in STD11 cold working die steel through confocal scanning laser microscopy; dilatometry; and X-ray diffraction analysis. The equilibrium phase diagram and phase fractions with temperature were calculated using a FactSage program. Confocal laser microscopic observation revealed that ${\alpha}$ to ${\gamma}$ transformation temperature is near $800^{\circ}C$; M7C3 carbides melt at $1245^{\circ}C$; and the melting temperature of STD11 is near $1370^{\circ}C$. XRD results indicated that the M23C6 carbides dissolve in the matrix if austenitized at over $1030^{\circ}C$; while the M7C3 carbides remain up to $1200^{\circ}C$ although their amount decreases. The calculated equilibrium phase diagram showed good agreement with experimental results on carbide dissolution and phase transformation temperatures.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.1 no.3
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• pp.27-32
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• 2008

Bone is a self-assembly material. It is known that the low amplitude and high frequency mechanical stimulus, which is much less amplitude but higher frequency than those induced by the normal activity, can induce new bone formation. The vibrating resonance is employed to elucidate why new bone is formed by this kind of mechanical stimulus. For example, as 30 Hz and $5{\mu}{\epsilon}$ mechanical stimulus is applied at the wall of canaliculus (the tiny tube type pathway of bone fluid flow and the diameter of canaliculus is less than 200nm), the osteocytic cell membrane experiences $1,000{\mu}{\epsilon}$ strain due to the vibrating resonance. Two experiments will follow after this pilot study; (1) observing the MAPK pathway of osteocytes by using in-vitro cell culture and (2) visualizing the actin filament network in the osteocytes by using the imaging technique, such as confocal laser scanning microscope.