Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Localization of Paclitaxel in Suspension Culture of Taxus chinensis

  • Choi, Hyung-Kyoon (Samyang Genex Biotech Research Institute) ;
  • Kim, Sang-Ic (Samyang Genex Biotech Research Institute) ;
  • Song, Jai-Young (Samyang Genex Biotech Research Institute) ;
  • Son (Samyang Genex Biotech Research Institute) ;
  • Hong, Seung-Suh (Samyang Genex Biotech Research Institute) ;
  • Durzan, Don-J. (Department of Environmental Horticulture, University of California at Davis) ;
  • Lee, Hyong-Joo (Department of Food Science and Technology, School of Agricultural Biotechnology, Seoul National University)
  • Published : 2001.06.01
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Abstract

The localization of paclitaxel was investigated in suspension culture cells of Taxus chinensis. Over 93% of the cell-associated paclitaxel were detected throughout the entire culture period. Intracellular localization of paclitaxel over the culture time was analyzed further by cell fractionation for days 21 and 42. Paclitaxel contents in intracellular organelles were decreased at day 42, while the content in the cell wall fraction was increased at day 42 compared to the value for day 21. The localization of paclitaxel in the cell wall was confirmed by using the immunocytochemical method with the aid of a confocal laser scanning microscope.

Keywords

References

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