• Title/Summary/Keyword: Scaffolding System

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A 3D bioprinting system and plasma-surface modification to fabricate tissue engineering scaffolds (조직공학용 세포담체 제작을 위한 플라즈마-표면개질이 포함된 바이오프린팅 시스템)

  • Kim, Geun-Hyeong
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2017.05a
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    • pp.3-23
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    • 2017
  • The achievement of tissue engineering can be highly depending on the capability to generate complicated, cell seeded three dimensional (3D) micro/nano-structures. So, various fabrication techniques that can be used to precisely design the architecture and topography of scaffolding materials will signify a key aspect of multi-functional tissue engineering. Previous methods for obtaining scaffolds based on top-down are often not satisfactory to produce complex micro/nano-structures due to the lack of control on scaffold architecture, porosity, and cellular interactions. However, a bioprinting method can be used to design sophisticated 3D tissue scaffolds that can be engineered to mimic the tissue architecture using computer aided approach. Also, in recent, the method has been modified and optimized to fabricate scaffolds using various natural biopolymers (collagen, alginate, and chitosan etc.). Variation of the topological structure and polymer concentration allowed tailoring the physical and biological properties of the scaffolds. In this presentation, the 3D bioprinting supplemented with a newly designed plasma treatment for attaining highly bioactive and functional scaffolds for tissue engineering applications will be introduced. Moreover, various in vivo and in vitro results will show that the fabricated scaffolds can carry out their structural and biological functionality.

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An Experimental Study on the Correlationship Between Highrised Safetyguard and Workers' Safety (안전난간유형에 따른 작업자 안전의 상관관계에 대한 실험적 연구)

  • 손기상
    • Journal of the Korean Society of Safety
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    • v.16 no.2
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    • pp.80-84
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    • 2001
  • Work stress and health are very important factors related to workers' condition and work safety, Author has conducted a couple of tests to check up three people's pulses before they work during their work for three types of safety guard in construction site. Work type, age, weight, sex experience year, ambient temperature n recorded as variables in this study. These are checked and asked by the author directly, before their pulse check Also, workers' Pulses were measured at height H-0 and H-9.0m to understand psychological the degree of psychological nervousness degree. POLAR protrainer NVTM HRM system(wrist recorder and sensor) was used for the site test. There are three(3) different types of construction safety guard which have been used for, this study. They are usually or frequently used and found in the site. Four different ranges of age groups(20's, 30's 40's, 50's) carpenters are assisted to the test sites with wearing pulse recorder and sensor respectively. All three types of safety guards have the same height 90㎝ even though they are different types. On top of five(5) stories tublar framed scaffolding is fabricated, similar to the one in site, where those safety guards are installed. The older(=more experienced) the workers are the more stable they are. it is shown. Also, It is verified the visually uncomfortable rail type can cause workers to psychologically be unstable.

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Wdpcp, a Protein that Regulates Planar Cell Polarity, Interacts with Multi‐PDZ Domain Protein 1 (MUPP1) through a PDZ Interaction (Planar cell polarity 조절단백질 Wdpcp와 multi-PDZ domain protein 1 (MUPP1)의 PDZ 결합)

  • Jang, Won Hee;Jeong, Young Joo;Choi, Sun Hee;Yea, Sung Su;Lee, Won Hee;Kim, Mooseong;Kim, Sang-Jin;Urm, Sang-Hwa;Moon, Il Soo;Seog, Dae-Hyun
    • Journal of Life Science
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    • v.26 no.3
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    • pp.282-288
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    • 2016
  • Protein-protein interactions regulate the subcellular localization and function of receptors, enzymes, and cytoskeletal proteins. Proteins containing the postsynaptic density-95/disks large/zonula occludens-1 (PDZ) domain have potential to act as scaffolding proteins and play a pivotal role in various processes, such as synaptic plasticity, neural guidance, and development, as well as in the pathophysiology of many diseases. Multi-PDZ domain protein 1 (MUPP1), which has 13 PDZ domains, has a scaffolding function in the clustering of surface receptors, organization of signaling complexes, and coordination of cytoskeletal dynamics. However, the cellular function of MUPP1 has not been fully elucidated. In the present study, a yeast two-hybrid system was used to identify proteins that interacted with the N-terminal PDZ domain of MUPP1. The results revealed an interaction between MUPP1 and Wdpcp (formerly known as Fritz). Wdpcp was identified as a planar cell polarity (PCP) effector, which is known to have a role in collective cell migration and cilia formation. Wdpcp bound to the PDZ1 domain but not to other PDZ domains of MUPP1. The C-terminal end of Wdpcp was essential for the interaction with MUPP1 in the yeast two-hybrid assay. This interaction was further confirmed in a glutathione S-transferase (GST) pull-down assay. When coexpressed in HEK-293T cells, Wdpcp was coimmunoprecipitated with MUPP1. In addition, MUPP1 colocalized with Wdpcp at the same subcellular region in cells. Collectively, these results suggest that the MUPP1-Wdpcp interaction could modulate actin cytoskeleton dynamics and polarized cell migration.

Fabrication and Evaluation of Hybrid Scaffold by Nano-Micro Precision Deposition System (나노-마이크로 정밀 분사 시스템을 이용한 하이브리드 인공지지체의 제작 및 평가)

  • Ha, Seong-Woo;Kim, Jong Young
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.38 no.8
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    • pp.875-880
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    • 2014
  • Recently, three-dimensional scaffolds and nanofibers are being developed for bone tissue regeneration. In this study, we fabricated a hybrid scaffold using a nano-micro precision deposition system. The fabrication process involved the application of the solid freeform fabrication (SFF) technology and electrospinning. The hybrid scaffolds were combined using micro scaffolds and nanofibers. The nanofibers were deposited on each layer of the micro scaffolding using the electrospinning process. The micro scaffolds were fabricated using the SFF technology at a temperature of $100^{\circ}C$, pressure of 650 kPa, and scan velocity of 250 mm/s. Nanofiber fabrication was conducted by means of electrospinning using the flow rate, solution concentration, distance from the tip to the collector (TCD), and voltage. The nanofibers were fabricated using a flow rate of 0.1 ml/min, voltage of 5 kV, TCD of 1 mm, and 10 wt% of solution concentration. MG-63 cells were seeded into the hybrid scaffold for the purpose of its evaluation.

An Exploration of Interaction Factors and Analysis on Interaction-Level of Synchronous Online Education in University (대학 실시간 온라인 교육에서의 상호작용 요소 탐색과 수준 분석)

  • Han, Hyeong-Jong
    • The Journal of the Korea Contents Association
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    • v.21 no.4
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    • pp.14-25
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    • 2021
  • The purpose of this study is to explore what are the interactive factors of synchronous online education in university and identify the level of interaction. This study used mixed research method. As a result of the interaction level, it was recognized that face-to-face education could be more interactive than synchronous online education. Synchronous online education could have better interactive between instructor and learner, and among learners than asynchronous online education. Factors which influencing the interaction were as follows: small group activities and scaffolding, diversification of communication channels and integration of learner's question in learning content. Detrimental elements were distance felt between instructor and learners, low intimacy among learners, content-focused lecture, restrictions on non-verbal communication, unstable systems and misusing microphones. The necessary factors to promote interaction are planning interactive class activities, etc. Based on the results, it was to suggest what kinds of efforts are needed to make interaction more effective in terms of teaching and learning method & activity, tool & system, and environment.

The Development of Education Model for CA-RP(Cognitive Apprenticeship-Based Research Paper) to Improve the Research Capabilities for Majors Students of Radiological Technology (방사선 전공학생의 연구역량 증진을 위한 인지적 도제기반 논문작성 교육 모형 개발)

  • Park, Hoon-Hee;Chung, Hyun-Suk;Lee, Yun-Hee;Kim, Hyun-Soo;Kang, Byung-Sam;Son, Jin-Hyun;Min, Jung-Hwan;Lyu, Kwang-Yeul
    • Journal of radiological science and technology
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    • v.36 no.2
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    • pp.99-110
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    • 2013
  • In the medical field, the necessity of education growth for the professional Radiation Technologists has been emphasized to become experts on radiation and the radiation field is important of the society. Also, in hospitals and companies, important on thesis is getting higher in order to active and cope with rapidly changing internal and external environment and a more in-depth expert training, the necessity of new teaching and learning model that can cope with changes in a more proactive has become. Thesis writing classes brought limits to the in-depth learning as to start a semester and rely on only specific programs besides, inevitable on passive participation. In addition, it does not have a variety opportunity to present, an actual opportunity that can be written and discussed does not provide much caused by instructor-led classes. As well as, it has had a direct impact on the quality of the thesis, furthermore, having the opportunity to participate in various conferences showed the limitations. In order to solve these problems, in this study, writing thesis has organized training operations as a consistent gradual deepening of learning, at the same time, the operational idea was proposed based on the connectivity integrated operating and effective training program & instructional tool for improving the ability to perform the written actual thesis. The development of teaching and learning model consisted of 4 system modeling, scaffolding, articulation, exploration. Depending on the nature of the course, consisting team following the personal interest and the topic allow for connection subject, based on this, promote research capacity through a step-by-step evaluation and feedback and, fundamentally strengthen problem-solving skills through the journal studies, help not only solving the real-time problem by taking wiki-space but also efficient use of time, increase the quality of the thesis by activating cooperation through mentoring, as a result, it was to promote a positive partnership with the academic. Support system in three stages planning subject, progress & writing, writing thesis & presentation and based on cognitive apprenticeship. The ongoing Coaching and Reflection of professor and expert was applied in order to maintain these activities smoothly. The results of this study will introduce actively, voluntarily and substantially join to learners, by doing so, culture the enhancement of creativity, originality and the ability to co-work and by enhance the expertise of based-knowledge, it is considered to be help to improve the comprehensive ability.

Cross-Sectional Item Response Analysis of Geocognition Assessment for the Development of Plate Tectonics Learning Progressions: Rasch Model (판구조론의 학습발달과정 개발을 위한 지구적 인지과정 평가의 횡단적 문항 반응 분석: Rasch 모델)

  • Maeng, Seungho;Lee, Kiyoung
    • Journal of The Korean Association For Science Education
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    • v.35 no.1
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    • pp.37-52
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    • 2015
  • In this study, assessment items to examine geocognition on plate tectonics were developed and applied to middle and high school students and college students. Conceptual constructs on plate tectonics are Earth interior structure, specific geomorphology, and geologic phenomena at each plate boundary. Construct for geocognition included temporal reasoning, spatial reasoning, retrospective reasoning, and system thinking. Pictorial data in each item were all obtained from GeoMapApp. Students' responses to the items were analyzed and measured cross-sectionally by Rasch model, which distinguishes persons' ability levels based on their scores for all items and compared them with item difficulty. By Rasch model analysis, Wright maps for middle and high school students and college students were obtained and compared with each other. Differential Item Functioning analysis was also implemented to compare students' item responses across school grades. The results showed: 1) Geocognition on plate tectonics was an assessable construct for middle and high school students in current science curriculum, 2) The most distinguished geocognition factor was spatial reasoning based on cross sectional analysis across school grades, 3) Geocognition on plate tectonics could be developed towards more sophisticated level through scaffolding of relevant instruction and earth science content knowledge, and 4) Geocognition was not a general reasoning separated from a task content but a content-specific reasoning related to the content of an assessment item. We proposed several suggestions for learning progressions for plate tectonics and national curriculum development based on the results of the study.

Exploring Topic-Specific PCK Progression for Elementary Teachers Instruction of Astronomy: Focusing on the Topic of Planet Size and Distance in Solar System (천문 수업에 대한 초등 교사의 주제-특이적 PCK 발달과정 탐색 -태양계 행성의 크기와 거리 주제를 중심으로-)

  • Lee, Kiyoung;Lee, Jeong-A
    • Journal of The Korean Association For Science Education
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    • v.36 no.4
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    • pp.629-641
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    • 2016
  • Understanding of how teachers change instruction can help predict what kind of educational materials is supportive or appropriate. On the basis of this idea, we explored elementary teachers' PCK progression on specific topics of astronomy: planet size and distance in solar system. To identify the development of PCK over time, we utilized learning progression (LP) as a conceptual framework. The progression of teacher PCK can also be illustrated as the hypothetical pathway from novice to expert like LP. Eight 5th grade elementary teachers participated in this study. We observed participating teachers' astronomy classes with the same topic. In order to document topic-specific PCK of participating teachers, we developed an analytic protocol consisting of four categories: knowledge of curriculum, knowledge of teaching strategies, knowledge of assessment, and astronomical thinking practice. In addition, we monitored the changes in the four participating teachers' PCK for two years in order to validate the evidences of the PCK progression. Participating teachers in this study took some intervention by attending a four-week pre-meeting with the researchers to profile an adaptive instruction. Through this research, we profiled four and five different levels of PCK progressions in three knowledge components (curriculum, teaching strategies, student assessment) and one astronomical thinking practice (systems thinking), respectively. Participating teachers demonstrated various levels and pathways in each component of PCK. This study released the empirical evidences in fostering instructional scaffolding, which is appropriate to the level of PCK of science teachers on specific topic.

How to build an AI Safety Management Chatbot Service based on IoT Construction Health Monitoring (IoT 건축시공 건전성 모니터링 기반 AI 안전관리 챗봇서비스 구축방안)

  • Hwi Jin Kang;Sung Jo Choi;Sang Jun Han;Jae Hyun Kim;Seung Ho Lee
    • Journal of the Society of Disaster Information
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    • v.20 no.1
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    • pp.106-116
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    • 2024
  • Purpose: This paper conducts IoT and CCTV-based safety monitoring to analyze accidents and potential risks occurring at construction sites, and detect and analyze risks such as falls and collisions or abnormalities and to establish a system for early warning using devices like a walkie-talkie and chatbot service. Method: A safety management service model is presented through smart construction technology case studies at the construction site and review a relevant literature analysis. Result: According to 'Construction Accident Statistics,' in 2021, there were 26,888 casualties in the construction industry, accounting for 26.3% of all reported accidents. Fatalities in construction-related accidents amounted to 417 individuals, representing 50.5% of all industrial accident-related deaths. This study suggests implementing AI chatbot services for construction site safety management utilizing IoT-based health monitoring technologies in smart construction practices. Construction sites where stakeholders such as workers participate were demonstrated by implementing an artificial intelligence chatbot system by selecting major risk areas within the workplace, such as scaffolding processes, openings, and access to hazardous machinery. Conclusion: The possibility of commercialization was confirmed by receiving more than 90 points in the satisfaction survey of participating workers regarding the empirical results of the artificial intelligence chatbot service at construction sites.

Stress Distribution on Construction Joint of Prestressed Concrete bridge Members with Tendon Couplers (텐던커플러를 사용한 프리스트레스트 콘크리트 교량부재의 이음부 응력분포 특성)

  • 오병환;채성태;김병석;이만섭
    • Journal of the Korea Concrete Institute
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    • v.13 no.1
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    • pp.1-8
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    • 2001
  • Recently, prestressed concrete(PSC) bridge structures with many repetitive spans have been widely constructed using the segmental construction method in many countries. In these segmentally constructed PSC bridges, there exist many construction joints which is required coupling of tendons or overlapping of tendons to introduce continuous prestress through several spans of bridges. The purpose of this paper is to investigate in detail the complicated stress distributions around the tendon coupled joints in prestressed concrete girders. To this end, a comprehensive experimental program has been set up and a series of specimens have been tested to identify the effects of tendon coupling. The present study indicates that the longitudinal and transverse stress distributions of PSC girders with tendon couplers are quite different from those of PSC girders without tendon couplers. It is seen that the longitudinal compressive stresses introduced by prestressing are greatly reduced around coupled joints according to tendon coupling ratios. The large reduction of compressive stresses around the coupled joints may cause deleterious cracking problems in PSC girder bridges due to tensile stresses arising from live loads, shrinkage and temperature effects. The analysis results by finite element method correlate very well with test results observed complex strain distributions of tendon coupled members. It is expected that the results of this paper will provide a good basis for realistic design guideline around tendon coupled joints in PSC girder bridges.