• Mycobiology
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• v.47 no.1
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• pp.97-104
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• 2019

Mushroom cultivation has gained increased attention in recent years. Currently, only four types of spawn, including sawdust spawn, grain spawn, liquid spawn, and stick spawn, are commonly available for mushroom cultivation. This limited spawn diversity has led to difficulty in selecting suitable inoculum materials in some cultivation. In this study, three small blocks of lignocellulosic agro-wastes and one block of a synthetic matrix were prepared as support for growing Pleurotus ostreatus in liquid medium. Mycelium-adsorbed blocks were then evaluated for their potential as block spawn for fructification. Our results indicated that the edible fungus was adsorbed and abundantly grew internally and externally on loofah sponge and synthetic polyurethane foam (PUF) supports and also has the ability to attach and grow on the surface of sugarcane bagasse and corncob supports. The mycelia of P. ostreatus adhered on corncob exhibited the highest metabolic activity, while those on the PUF showed the least activity. Mycelial extension rates of block spawns made of agro-waste materials were comparable to that of sawdust spawn, but the block spawn of PUF showed a significantly lower rate. No significant differences in cropping time and yield were observed among cultivations between experimental block spawns and sawdust spawns. Moreover, the corncob block spawn maintained its fruiting potential during an examined period of 6-month storage. The developed block spawn could be practically applied in mushroom cultivation.

• The Korean Journal of Mycology
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• v.49 no.1
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• pp.57-66
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• 2021

Six strains among the shiitake varieties developed by the National Institute of Forest Science were used to select suitable varieties for sawdust block cultivation method. After 100 days cultivation (60 days in dark and 40 days in light), the weight loss rate of the sawdust block media was 14.4% (12.8% to 15.7%) in average. After 5 to 7 days of fruiting process, 2~36 primordia per medium were formed. The number of primordium formation was in the following order: Manchuhyang> Sansanhyang> Sanbaekhyang> Bambithyang> Sangjanghyang> Taehyanggo. The stipe length was 41~59 mm as followed by Taehyanggo (59 mm), Sanbackhyang (56 mm), Bambithyang (54 mm), Manchuhyang (53 mm), Sanjanghyang (45 mm) and Sansanhyang (41 mm). Among the strains, Sansanhyang had short and thick stipe. The average weight of fruiting bodies was 33~77 g as following order of Taehyanggo (77 g), Bambithyang (51 g), Sansanhyang (50 g), Manchuhyang (41 g), Sanjanghyang (34 g) and Sanbackhyang (33 g). Mushroom production per 2 kg medium was the highest in Sanbackhyang (473 g) followed by Bambithyang (287 g), Manchuhyang (175 g), Sansanhyang (109 g), Sanjanghyang (78 g) and Taehyanggo (60 g). The hardness of the cap was 1,218 to 1,839 g/10 mm. Taehyanggo was the hardest (1,839 g/10 mm) and Bambithyang was the softest (1,218 g/10 mm). In conclusion, in the cultivation condition of sawdust block medium, mushroom production was good in Sanbackhyang and Bambithyang. Sansanhyang showed good in terms of fruiting body shape.

• The Korean Journal of Mycology
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• v.51 no.2
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• pp.81-90
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• 2023

The cultivation method of shiitake is divided into two methods: log cultivation and sawdust cultivation. Recently, the importance of sawdust cultivation has been highlighted due to problems such as environmental problems, rising labor costs, and a reduced labor force. Among the 24 Sawdust-cultivated Lentinula edodes cultivars in Korea stored by the National Institute of Forest Science, this study was conducted to select excellent cultivars under the sawdust block cultivation method. After inoculation, the sawdust mediums were cultured for 100 days (60 days in dark and then 40 days in light), and only Sanjo 712ho sprouted the primordia on the 10^th day of light culture (total 70 days of cultivation). As a result of cultivation, the average total yield of the 24 cultivars were 1,816 g and Sanjo 712ho was 2,267 g. The fruiting body yield was the highest in Sanjo 713ho with 3,443 g followed by Sanjo 710ho (3,355 g). Sanlim 10ho, Sansanhyang, and Sanjo 716ho showed low production with 174, 238, and 214 g, respectively. As a result of investigating the morphological characteristics of the fruit bodies, the fresh weight of Sansanhyang and Bambithyang was about twice as heavy as the overall average, and Sulbaekhyang was about twice as light. Bambithyang was the largest and longest in diameter and length of the stipe, and the thickness of the stem was the thickest in Sanjo 716ho. As for the hardness of pileus, Bambithyang showed the highest value at 1,276 g/5 mm, and Sanjo 711ho showed the lowest value at 542 g/5 mm. In summary, Bambithyang showed the best fruiting body characteristics and Sanjo 713ho showed the highest yield in the sawdust block cultivation method. This study, the cultivar with shortened cultivation periods and cultivars with excellent morphological characteristics and high yields.

• Journal of the Korean Wood Science and Technology
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• v.38 no.2
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• pp.124-134
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• 2010

This study was performed to evaluate the efficiency of using softwood as the sawdust medium for Lentinula edodes cultivation, effect of nutrient on the mycelial growth, spawning, the mushroom yield, and quality. The nitrogen nutrition significantly enhanced the mycelial growth of L. edodes. The glutamic acid in the L. leptolepis and P. koraiensis, and asparagine in the P. densiflora were appeared to slight increase in the mycelial growth. The vegetable oil showed very effective on the mycelial growth in the P. koraiensis sawdust medium. Carbon/nitrogen ratio of all the test was reduced after mycelial growth. The mycelial growth was exclusively dependent on reduction of carbon. The mushroom yield (32.7%) of the P. densiflora sawdust medium (carbon source: 3% active carbon, nitrogen source: 0.4% asparagines) was the best in mushroom production of L. edodes, followed by the Q. variabilis sawdust (35.4%) of the control medium. The diameter of mushroom cap was obtained from the P. densiflora sawdust (carbon source: 3% sucrose, nitrogen source: 0.4% potassium nitrate) and P. koraiensis sawdust (carbon source: 3% sucrose, nitrogen source: 0.4% potassium nitrate), and the P. koraiensis sawdust (carbon source: 3% xylose, nitrogen source: 0.4% glutamic acid, supplement: 0.05% amino acid), with values 71.5 mm, 71.5 mm and 72.1 mm, respectively. In the polypropylene bag cultivation, the weight losses of the block medium gradually increased for 80 days in the dark (13.8~16.8%) and then became stable in the range of 20.7~25.8%.

• The Korean Journal of Mycology
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• v.44 no.2
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• pp.100-102
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• 2016

Ergothioneine is a natural compound with strong antioxidant properties. In this study, the effects of different nitrogen sources including ammonium nitrate, ammonium sulfate, sodium nitrate, and histidine in sawdust media were investigated to enhance ergothioneine contents in Shiitake (Lentinula edodes) fruit bodies. The addition of 0.2% ammonium sulfate in the growth media showed the highest enhancement of ergothioneine content in shiitake fruit bodies which was 1.7-fold higher than the control. On the other hand, histidine, a building block of ergothioneine decreased the concentration of ergothioneine significantly. Our results demonstrate that the cultivation of shiitake in sawdust media with suitable nutrients was effective to enhance its ergothioneine contents.

• Journal of Mushroom
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• v.5 no.1
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• pp.29-33
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• 2007

This study for establishment of cultivation technique was carried out in home, Pleurotus ostreatus, Ganoderma lucidum. The mushroom kit allows you to grow some of the mushrooms used by the finest chefs in the world easily and quickly almost anywhere in your own home or office. Mushroom kits may be placed on a coffee table, counter, or desk. They will produce mushrooms virtually anywhere room temperature is maintained. Now we have the opportunity not only to enjoy watching these exotic mushrooms grow but also to enjoy eating the freshest organic mushrooms possible. To study for the possibility of indoor cultivation of P. ostreatus, and G. lucidum, we invested cultivation status with two kinds of sawdust, cotton waste and rice straw in washroom, kitchen, living room, and bedroom. It took 2~4 days to make primordia formation in a block of P. ostreatus, and G. lucidum was 11 days. Weight of P. ostreatus on cotton waste, rice straw, and willow sawdust were 2,060g, 90g, and 770g, respectively. and weight of G. lucidum on oak sawdust was 172g. Th best result was achieved in washroom, among used washroom, kitchen, living room, and bedroom.

• The Korean Journal of Mycology
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• v.51 no.4
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• pp.429-440
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• 2023

Wild Lentinula edodes (shiitake) strains are an important breeding material for developing new varieties suitable for various consumer preferences and environmental conditions. The morphological characteristics of 100 wild shiitake strains collected from 14 regions were investigated in a sawdust block cultivation. The characteristics of their fruiting bodies were diverse. Among them, 38% of the caps was convex, 33% was broadly umbonate, 27% was applanate, and 2% was shallowly depressed. In terms of their stipes, 55, 23, and 22% were the column, clavate, and funnel type, respectively. The shape of the cap was the most diverse on Mt. Hwaak, whereas the shape of the stipe showed various phenotypes from various regions. Even among strains collected from the same forest, different types of mushrooms were observed. In conclusion, wild shiitake strains that have adapted to various environmental stress can be used to develop new varieties. Thus, securing various genetic resources and studying their traits are important for researching breeding technology.