• YAKHAK HOEJI
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• v.43 no.1
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• pp.23-27
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• 1999

We previously reported that the extract of unripe cotton ball of Gossypium indicum shows cytotoxic activity and induces an apoptosis in vitro. In present study, its antitumor activity was investigated in vivo. Butanol soluble extract of unripe cotton ball reduced the weight of solid tumor in sarcoma 180 transplanted mouse model. It showed stronger activity than that of Krestin.

• Archives of Pharmacal Research
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• v.8 no.4
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• pp.283-284
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• 1985

It was previously reported that the petroleum ether fraction of the Korean ginseng root shows cytotoxic activities against L1210, L5178Y, Hela cell and Sarcoma 180 cell (1). In this study the cytotoxic substance against L1210 cell was isolated over a silica gel column and a preparative HPLC, followed by the cytotoxic assay (2).

• YAKHAK HOEJI
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• v.43 no.5
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• pp.629-634
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• 1999

A protein-polysaccharide fraction was isolated from the mycelial culture of a Korean basidiomycetous fungus, Hebeloma crustuliniforme, and was named HCA (Hebeloma crustulineforme fraction A). When intraperitoneally injected at 200 mg/kg/day once daily for 10 days, HCA inhibited the growth of sarcoma 180 solid tumor in ICR mice by 65.1% (p＜0.05). HCA also exerted protective effect against leukopenia induced by cyclophosphamide with the protection ratio of 39.7% (p＜0.01).

• The Korean Journal of Mycology
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• v.11 no.4
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• pp.159-162
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• 1983

The dried carpophores of Sarcodon aspratus were examined for mineral elements and a protein-polysacccharide. Calcium, iron, zinc, magnesium, manganese, copper, lead, cadmium and mercury were detected in that order of contents by atomic absorption spectrometry and automatic mercury analysis. The protein-polysaccharide fraction extracted from the carpophore was found to contain 31.5% protein and 52.8% polysaccharide. The fraction showed no antitumor activity against sarcoma 180 implanted in mice.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.205-206
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• 2001

녹조류 중 갈파래(Ulva sp.)는 유럽에서도 오래전부터 식용으로 이용되어 다양한 식품학적 및 생리 기능성에 관한 연구가 진행되어 오고 있다. 갈파래의 주요 특성의 하나는 육상 식물과 달리 황산기를 함유한 다당을 다량 함유하고 있으며, 이 산성 다당은 항종양성, 항 virus성, 면역활성증간 항혈액 응고작용 등이 보고되어 있으며, 국내에서도 홑파래에서 추출한 당단백질이 Sarcoma-180 cell에 대한 항암효과 및 면역활성이 있다고 보고된 바 있다(이 등, 1992). (중략)

• Bulletin of the Korean Chemical Society
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• v.14 no.2
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• pp.176-178
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• 1993

A series of 4-(2-chloroethyl)nitrosocarbamoyl- and 4-methyl nitrosocarbamoyl analogs of 4-Aza-$5{\alpha}$-cholestane (5a and 5b), 4-Aza-5-cholestene (6a and 6b) were synthesized and evaluated for their inhibitory activity against the Sarcoma 180 cell. The steroidal nitrosoureas, 5a, 5b, 6a and 6b displayed modest activity in vitro.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.4
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• pp.424-430
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• 2007

This study was conducted to investigate the effect of mycelia of Lentinus edoes mushroom-cultured Lonicera japonica Thunberg (LLJ) on proliferation of the cancer cell lines (Hep3B, MCF-7 and HeLa), sarcoma 180 (S-180) and antiallergy. In an anti-cancer test using Hep3B (hepatic cancer cell), MCF-7 (breast cancer cell) and HeLa (uterine cancer cell), LLJ extract showed higher antiproliferating effect than that of LJ (Lonicera japonica Thunberg) extract. In an anti-cancer testing using Hep3B cells, LLJ extract showed growth-inhibitory effect of $85.60{\pm}4.66%$ at 3mg/rnL. In an anti-cancer testing using MCF-7 cells, LLJ and LJ extracts showed high antiproliferating effect. LLJ showed the tumor suppressive effect in mice injected with S-180 cells. The growth-inhibitory rates against tumor cells were 61% for LLJ, 37% for LJ. LLJ inhibited histamine release from rat peritoneal mast cells activated by compound 48/80. These results suggest that Lentinus edodes mushroom-cultured herb has an antiproliferating effect against cancer cell lines (Hep3B, MCF-7 and HeLa) and S-180 tumor, and will be beneficial in the treatment of allergic reaction.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.1
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• pp.8-13
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• 2007

This study was conducted to investigate the effect of mycelia of Lentinus edoes mushroom-cultured Astragalus membranaceus Bunge (LAM) on proliferation of cancer cell lines (Hep3B, MCF-7 and HeLa), sarcoma 180 (S-180), and anti-allergy. In an anti-cancer test using Hep3B (hepatic cancer cell), MCF-7 (breast cancer cell) and HeLa (uterine cancer cell), LAM extract showed higher antiproliferating effect than that of Astragalus membranaceus Bunge (AM) extract. In an anti-cancer testing using Hep3B cells and MCF-7 cells, LAM extract showed growth-inhibitory effect of 65.23% at 3 mg/mL and 69.23% at 5 mg/mL, respectively. In an anti-cancer testing using HeLa cells, LAM extract showed growth-inhibitory effect of 42.01% at 5 mg/mL. In addition, LAM showed the tumor suppressive effect in mice injected with S-180 cells. The growth-inhibitory rates against tumor cells were 47% for LAM and 37% for AM. LAM inhibited histamine release from rat peritoneal mast cells activated by compound 48/80. These results suggest that Lentinus edodes mushroom-cultured herb has an antiproliferating effect against cancer cell lines (Hep3B, MCF-7 and HeLa), S-180 tumor and will be beneficial in the treatment of allergic reaction.

• Archives of Pharmacal Research
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• v.2 no.2
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• pp.145-151
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• 1979

The carpophores of three Korean mushrooms, Coriolus versicolor, Pleurotus ostreatus and Lentinus edodes were respectively extracted with hot water and the extracts were dialyzed through Visking tube. They were found to exert an antitumor activity against sarcoma-180 implanted in mice. Especially, the inhibition ratio of the extract of Coriolus versicolor (100 mg/ Kg.i.p) was almost 100% . But all the extracts did not affect the growth of leukemia L517Y cells in vitro. Therefore these facts indicate that the extracts appear to stimulate cell-mediated immunity.

• The Korean Journal of Mycology
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• v.11 no.4
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• pp.147-150
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• 1983

To investigate a possibility of producing the antitumor component by shake culture method, the mycelia of Flammulina velutipes were cultured in flasks on a shaker at $26{\sim}28^{\circ}C$ at 180 rpm for seven days. The extract of the mycelia was concentrated under vacuum. The precipitate obtained by adding a three-fold volume of ethanol was centrifugated and freeze-dried after dialysis. The fraction was tested against sarcoma 180 in the mice. The inhibition ratio of the fraction against the tumor was 68.0% at the dose of 20 mg/kg/day for the period of ten days and the tumors in three of the mice were completely regressed. The results showed, therefore, that the antitumor component was produced by the shake culture method.