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Regulation of Chilling Tolerance in Rice Seedlings by Plant Hormones

  • Chu, Chun;Lee, Tse-Min
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.3
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    • pp.288-298
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    • 1992
  • Since the major important factors limiting plant growth and crop productivity are environmental stresses, of which low temperature is the most serious. It has been well known that many physiological processes are alterant in response to the environmental stress. With regard to the relationship between plant hormones and the regulation of chilling tolerance in rice seedlings, the major physiological roles of plant hormones: abscisic acid, ethylene and polyamines are evaluated and discussed in this paper. Rice seedlings were grown in culture solution to examine the effect of such plant hormones on physiological characters related to chilling tolerance and also to compare the different responses among tested cultivars. Intact seedlings about 14 day-old were chilled at conditions of 5$^{\circ}C$ and 80% relative humidity for various period. Cis-(+)-ABA content was measured by the indirect ELISA technique. Polyamine content and ethylene production in leaves were determined by means of HPLC and GC respectively. Chilling damage of seedlings was evaluated by electrolyte leakage, TTC viability assay or servival test. Our experiment results described here demonstrated the physiological functions of ABA, ethylene, and polyamines related to the regulation of chilling tolerance in rice seedlings. Levels of cis-(+)-ABA in leaves or xylem sap of rice seedlings increased rapidly in response to 5$^{\circ}C$ treatment. The tolerant cultivars had significant higher level of endogenous ABA than the sensitive ones. The ($\pm$)-ABA pretreatment for 48 h increased the chilling tolerance of the sensitive indica cultivar. One possible function of abscisic acid is the adjustment of plants to avoid chilling-induced water stress. Accumulation of proline and other compatible solutes is assumed to be another factor in the prevention of chilling injuies by abscisic acid. In addition, the expression of ABA-responsive gene is reported in some plants and may be involving in the acclimation to low temperature. Ethylene and its immediate precusor, 1-amincyclopropane-1-carboxylic acid(ACC) increased significantly after 5$^{\circ}C$ treatment. The activity of ACC synthase which converts S-adenosylmethionine (SAM) to ACC enhanced earlier than the increase of ethylene and ACC. Low temperature increased ACC synthase activity, whereas prolonged chilling treatment damaged the conversion of ACC to ethylene. It was shown that application of Ethphon was beneficial to recovering from chilling injury in rice seedlings. However, the physiological functions of chilling-induced ethylene are still unclear. Polyamines are thought to be a potential plant hormone and may be involving in the regulation of chilling response. Results indicated that chilling treatment induced a remarkable increase of polyamines, especially putrescine content in rice seedlings. The relative higher putrescine content was found in chilling-tolerant cultivar and the maximal level of enhanced putrescine in shoot of chilling cultivar(TNG. 67) was about 8 folds of controls at two days after chilling. The accumulation of polyamines may protect membrane structure or buffer ionic imbalance from chilling damage. Stress physiology is a rapidly expanding field. Plant growth regulators that improve tolerance to low temperature may affect stress protein production. The molecular or gene approaches will help us to elucidate the functions of plant hormones related to the regulation of chilling tolerance in plants in the near future.

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A Study on Development of Safety Management Evaluation Index for Shipping Company (해운선사 안전관리 평가지표 개발에 관한 연구)

  • Kim, Hwa-Young
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.19 no.6
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    • pp.629-636
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    • 2013
  • There is no safety evaluation index with marine accidents and information of ship's safety management result for good grasp of safety management level of shipping company and effective monitoring. In this paper, we proposed a SMEI(Safety Management Evaluation Index) for evaluation of shipping companies. The SMEI is consist of MAR(marine accidents ratio), SSMR(ship safety management ratio) and SAP(safety advantage point) through brainstorming of the expert group and AHP(analysis hierarchy process)in previous study. And weights of SMEI are calculated using experts's opinions through the Delphi method. We also verified the validity of proposed SMEI to 119 Korean shipping companies with 916 ocean-going ships of Korean flag, which grouped three part (small, mid and big) by number of ship and gross tonnage. And we found out 19 shipping companies, 8 companies in group1 & group2 respectively and 3 companies in group 3, with score below 1.0 by SMEI. In these companies, there are no marine accidents in recent 3 years and appeared low frequency remarkably in the detention and mojor non-conformity by data analysis.

Construction stages analyses using time dependent material properties of concrete arch dams

  • Sevim, Baris;Altunisik, Ahmet C.;Bayraktar, Alemdar
    • Computers and Concrete
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    • v.14 no.5
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    • pp.599-612
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    • 2014
  • This paper presents the effects of the construction stages using time dependent material properties on the structural behaviour of concrete arch dams. For this purpose, a double curvature Type-5 arch dam suggested in "Arch Dams" symposium in England in 1968 is selected as a numerical example. Finite element models of Type-5 arch dam are modelled using SAP2000 program. Geometric nonlinearity is taken into consideration in the construction stage analysis using P-Delta plus large displacement criterion. In addition, the time dependent material strength variations and geometric variations are included in the analysis. Elasticity modulus, creep and shrinkage are computed for different stages of the construction process. In the construction stage analyses, a total of 64 construction stages are included. Each stage has generally $6000m^3$ concrete volume. Total duration is taken into account as 1280 days. Maximum total step and maximum iteration for each step are selected as 200 and 50, respectively. The structural behaviour of the arch dam at different construction stages has been examined. Two different finite element analyses cases are performed. In the first case, construction stages using time dependent material properties are considered. In the second case, only linear static analysis (not considered construction stages) is taken into account. Variation of the displacements and stresses are obtained from the both analyses. It is highlighted that construction stage analysis using time dependent material strength variations and geometric variations has an important effect on the structural behaviour of arch dams. The maximum longitudinal, transverse and vertical displacements obtained from construction stages and static analyses are 1.35 mm and 0 mm; -8.44 and 6.68 mm; -4.00 and -9.90 mm, respectively. In addition, vertical displacements increase from the base to crest of the dam for both analyses. The maximum S11, S22 and S33 stresses are obtained as 1.60MPa and 2.84MPa; 1.39MPa and 2.43MPa; 0.60MPa and 0.50MPa, respectively. The differences between maximum longitudinal, transverse, and vertical stresses obtained from construction stage and static analyses are 78%, 75%, and %17, respectively. On the other hand, there is averagely 12% difference between minimum stresses for all three directions.

Bibliographical studies on the shashen(Adenophora triphylla var. Japonica Hara.) (사삼(沙蔘)에 관(關)한 문헌적(文獻的) 고찰(考察))

  • Shin, Dong Hun;Seo, Young Bae
    • Journal of Haehwa Medicine
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    • v.8 no.2
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    • pp.107-122
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    • 2000
  • As a result of studing on the "shashen(沙蔘)", we could reach conclusions as follows. 1. Shashen is the root of "lunyeshashen(潤葉沙蔘)", "kuoyeshashen(闊葉沙蔘)", and its same generic plants that belong to Companulaceae. In china people has used twenty-four species as a shashen together with other generic plants, in korea sixteen species as a shashen. 2. At first, in the "Shen Nong's Herbal(神農本草經)" it was recorded and then in the "Benchingfengyuan(本經逢原)" divided into "nanshashen(南沙蔘)" and "beishashen(北沙蔘)". Recently, nanshashen called shashen belongs to Companulaceae and have strong efficacies of clearing away heat from lung, resolving phlegm, beishashen belongs to Umbelliferae and have strong efficacies of nourishing stomach, nourishing yin. 3. Shashen is the root of Adenophora stricta Miq., and yangru(羊乳) is that of Codonopsis lauceolata Bentham et Hooker, jini is that of Adenophora remotiflora (SIEBOLD et ZUCCARINI.) MIQUEL. From a efficacious point of view, it is the characteristics that shashen have efficacies of nourishing yin and clearing away heat from lung, supplementing stomach and promoting production of body fluid, resolving phlegm and relieving cough, eliminating pus and abscess, expelling wind and pruritus, jini have those of clearing away heat and toxic material, resolving phlegm and yanru have those of nourishing yin and moistening lung, resolving phlegm and eliminating pus, clearing away heat and toxic material, stimulating milk secretion. 4. After being recorded as "zhimu(知母)" in Shen Nong's Herbal, the alias of shashen was recorded as kuxin(苦心), shimei(識美), huxu(虎須), baishen(白參), zhiqu(志取), wenhu(文虎), baolishen(保利參), paoshen(泡參), jibantui(鷄半腿), yangponai(羊婆妨) and so on. Moreover shashen was named after its characteristics of that it grows well in the sandy soil and as a wushen(五參) with a renshen(人蔘) its form is different from that of wushen but their chief virtues are alike. 5. In the numerous medical books, xinyeshashen(杏葉沙蔘) regarded as nanshashen. It was called as the alias of jini, so I thouhgt that it was wrong xinyeshashen to be regarded as nanshashen. 6. It was used shashen for renshen, renshen was used to treat lung-cold syndrome by its efficacy of tonifying yang and shashen lung-heat syndrome by its efficacy of nourishing yin, the reasons of that because shashen had amount of sap, its properies and flavours of herbs are light and clean. 7. The constituents of shashen were essential oil, starch, shashen-saponin, furocoumarin, xanthotoxin(ammoidin), inulin, sugar, mucus and have efficacies of resolving phlegm, promoting production of body fluid, immunomodulational and antibiotic efficacies. above results indicated that the origin of shashen and substitutional plants was various. Their efficacies are somewhat alike, but there were characteristic efficacies each other. Nowadays they are used together with, so we should know the characteristic efficacies of them and then we using them clinically, more deep discrimination and experimental support shoud be accomplished.

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Directional and Orthogonal Effects of Seismic Loads on Design Member Forces (설계부재력에 대한 지진하중의 방향 및 직교성 영향)

  • Ko, Dong-Woo;Jeong, Seong-Wook;Lee, Han-Seon
    • Journal of the Earthquake Engineering Society of Korea
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    • v.9 no.3 s.43
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    • pp.51-58
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    • 2005
  • Many RC building structures of multiple uses constructed in Korea have the Irregularities of torsion and soft story at bottom stories simultaneously. Seismic design codes generally require dynamic analysis and to take into account the effect or earthquake excitations in the orthogonal direction using the approximate methods of 100/30 and SRSS for type of building structures. And ail buildings should be designed to be safe in any direction of earthquake input. But, most of designers have difficulty in considering the orthogonal and directional effect of earthquake. The objective of the study stated herein is to verily 1) the effect of the choice of the reference axes on the seismic design member forces by comparing the analytical results on member forces using the principal axes suggested by Wilson and the global axes generally adopted in design office, 2) the validity or the 100/30 and SRSS methods by comparing the member forces obtained through linear elastic time history analysis with those obtained through using response spectrum analysis and 100/30 (or SRSS) methods. Based on the observations on the analytical results, it is concluded as follows; 1) The values of member forces by principal axes can be about $15\%$ smaller than those by the global axes in the example structure. 2) Though the values of member forces given by time history analysis are generally within the peak values predicted by 100/30 and SRSS methods, many member force vectors $(P,\;M_y,\;and\;M_z)$ by lime history analysis were located outside the boundaries predicted by the approximate method such as the 100/30 method.

Influential Factors of Digital Customer Experiences on Purchase in the 4th Industrial Revolution Era - Focusing on Moderated Mediating Effects of Digital Self Efficacy- (4차 산업혁명시대의 디지털 고객경험과 구매간 영향관계 - 디지털 자기효능감의 조절된 매개효과를 중심으로-)

  • Jung, Sang Hee;Chung, Byoung Gyu
    • Journal of Venture Innovation
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    • v.3 no.1
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    • pp.101-115
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    • 2020
  • In the era of the 4th Industrial Revolution customers living began to come out, not inside the purchase funnel. Due to the diversity of product selection and the increase in digital channels, the way customers search for information and purchase it is changing innovatively. So, the customer journey in the digital age is much more complicated than the traditional funnel model suggests. Unlike many previous studies, this study was conducted for 1,200 customers in four product groups of fashion, automobile, cosmetics, and online shopping malls. As a result of the study, we investigated how digital self-efficacy plays a role in purchasing in a series of processes in which digital experience affects customer satisfaction and finally affects purchase. As a theoretical implication, as a result of introducing and testing digital self efficacy as moderated mediation effect. the digital self-efficacy between customer satisfaction and customer loyalty were determined to play a moderated mediation effect role. As a practical implication, it was necessary to actively utilize digital marketing for customers with high digital self-efficacy, but it was suggested that customers with low digital self-efficacy need to be careful about digital marketing fatigue.

The Methodology for Environmental Risk Assessments of Non-target Organisms (Insects) on LM Rice (LM벼에 영향을 받는 비표적 생물체(곤충)에 대한 환경위해성 평가 (ERA) 방법)

  • Yi, Hoonbok;Kim, Hyun-jung;Na, Sumi
    • Korean Journal of Environment and Ecology
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    • v.30 no.4
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    • pp.712-723
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    • 2016
  • This paper is to suggest the methods about the environmental risk assessment (ERA) based on non-target insect species for LM rice crop produced by biotechnology. We used some data by the picture dictionary of agricultural pests in Korea, some books for ERA or rice pest ecology, articles about rice ecology, and internet sites and many specialists gave us good advice for this ERA system. We found about 140 insect species using rice crop as their habitat and we discriminated herbivores into Sap feeder, Grain feeder, Defoliator, and Pollen feeder according to their food functional group. We also clarified the potential damage possibility of insect species from LM rice crop. Then, we ranked 10 non-target insect species (Baliothrips biformis, Laodelphax striatellus, Nilaparvata lugens, Mythimna separata, Cnaphalocrocis medinalis, Cletus schmidti Kiritshenko, Scotinophara lurida, Nilaparvata bakeri, Oxya chinensis, Agromyza oryzae) with giving some scores using Geographic distribution, Habitat specialization, Prevalence on crop, Abundance. We showed some environmental risk hypotheses about herbivore, predator, and parasitoid, and infered the impacts of non-target species on LM rice crop. Conclusively, we want to serve this methodology to build the biosafety system from biotechnology crops, and the methodology is to keep public health, sustain the biodiversity, and conserve the natural ecosystems.

Influence of ACLSV-infection on Fruit Quality of 'Hongro' Apples (ACLSV(Apple chlorotic leafspot virus) 감염이 사과 '홍로' 품종의 과실품질에 미치는 영향)

  • Kim, Hyun-Ran;Kim, Jeong-Soo;Hwang, Jeong-Hwan;Lee, Sin-Ho;Choi, Gug-Seon;Choi, Yong-Mun
    • Research in Plant Disease
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    • v.10 no.2
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    • pp.145-149
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    • 2004
  • 'Hongro' is one of the most important apple cultivars whose growing area is increasing because of its good quality. Recently fruit shrinking symptom causing decrease of fruit size, juice, and quality, appears in some commercial 'Hongro' orchards. The average frequency of occurrence of fruit shrinking symptom was 12 % of total trees investigated and Apple chlorotic leaf spot virus (ACLSV) was detected from all the trees showing fruit shrinking symptom by ELISA. A typical virus infection symptom of leaf epinasty and stem necrosis appeared on woody indicators, Spy227 and Virginia crab grafted with infected trees and all the grafted trees showed positive reaction to ACLSV antiserum by ELISA. It was proved that ACLSV can be easily transmitted by grafting. ACLSV was also isolated from the leaves of C. quinoa inoculated with sprouting leaf sap of infected trees. To prove that the fruit shrinking symptom was caused by ACLSV infection, ACLSV-infected scion was grafted on virus-free 'Hongro/M9' and the fruit characteristics were investigated. Consequently the same symptoms of fruit size and juice decreasing were observed from the trees grafted with ACLSV-infected scion. Therefore, it is suggested that the fruit shrinking symptom is caused by ACLSV infection and 'Hongro' can be classified as sensitive cultivar to ACLSV.

Predicting Influence of Changes in Indoor Air Temperature and Humidity of Wooden Cultural Heritages by Door Opening on Their Conservation Environment (개방에 따른 실내 온습도 변화가 목조문화재 보존환경에 미치는 영향 예측)

  • Kim, Min-Ji;Shin, Hyun-Kyeong;Choi, Yong-Seok;Kim, Gwang-Chul;Kim, Gyu-Hyeok
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.6
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    • pp.798-803
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    • 2015
  • This study was conducted to predict the effect of door opening in wooden cultural heritages (WCHs) on their conservation environment. For this prediction, measured relative humidity (RH) and surface wood moisture content (MC) of inner part of wood columns in open wooden building and neighboring closed wooden building were compared with minimum RH, including the duration of minimum RH, and MC required for spore germination and resultant growth of wood-degrading fungi reported in some literatures. Moisture conditions, namely RH of inside wooden building and MC of wood was unsuitable for decay and sap-stain fungi all the year round; however, moisture conditions during summer season was suitable for spore germination and resultant growth of surface mold fungi, regardless of door opening. When compared, the duration of minimum (75%) or higher RH and the number of wood columns with MC level greater than the minimum MC (15%) during summer season, the surface mold related to the conservation environment of inside wooden building was somewhat better in open building than in closed building. Rather, doors should be opened in closed building for reducing indoor RH as a necessary measure during summer season when outdoor RH is high.

Development of self-sealing waterproof materials using GRT powder (폐타이어 분말을 이용한 자체보수성 방수재 개발)

  • Lee Dong-Min;Choi Joong-So
    • Resources Recycling
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    • v.14 no.4 s.66
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    • pp.22-33
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    • 2005
  • Four sheet-shaped and one soft-shaped self-sealing waterproof materials were prepared to recycle some GRT(Ground Rubber Tires). Their physical properties were tested to consider characteristics of them. The self-sealing waterproof materials were consisted of GRT/super absorbent polymer(SAP)/binder and mold by a hot press after mixing with a batch-typed internal mixer. The average size of GRT particles was -40 mesh, SAPs were commercial GE-500F and poly(AM-SAS-AA) prepared in this work. Binders were PU. EVA, LDPE, SBR, and poly(2-EHA). And PU film was attached to improve the properties of waterproof materials. Characteristics of self-sealing waterproof materials consisted of by GRT/GE-500F/EV-600/PU film and GRT/GE-500F/SBR(vulcanization)/PU film among the developed self-sealing waterproof materials were similar to the commercial products. And properties of the soft-shaped self-sealing waterproof materials consisted of by GRT/GE-500F/Po1y(2-EHA) and CRT/Poly(AM-SAS-AA)/poly(2-EHA) were improved within from four times to twenty times compared to the one oi the commercial products.