• Title/Summary/Keyword: Sample pre-treatment

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The Effects of Character Education Program based on Self-reflection Using a Liberal-arts Course at University (대학 교양강좌를 이용한 자기성찰 기반 인성교육 프로그램의 효과)

  • Kim, Eunjin
    • The Journal of the Korea Contents Association
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    • v.22 no.2
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    • pp.294-304
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    • 2022
  • The purpose of this study is to examine the effects of a character education program based on self-reflection using a liberal arts course at university. To this end, a single treatment group pre- and post-design was conducted in which data were collected at the beginning and end of the semester for 18 students. For statistical estimation suitable for a small sample size, a non-parametric test was performed to examine the significant mean changes of the data. This program was found to significantly improve the level of self-compassion, life satisfaction, and mental well-being of university students. Based on the above results, it was discussed that the liberal arts course for character education based on self-reflection could be useful as a character education for university students, and the implications and suggestions of the results of this study were presented.

Physicochemical Characteristics of Sweet Persimmon by Heating Treatments (가열처리에 의한 단감의 이화학적 특성)

  • 손규목;김광호;성태수;김종현;신동주;정지영;배영일
    • The Korean Journal of Food And Nutrition
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    • v.15 no.2
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    • pp.144-150
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    • 2002
  • Sweet persimmon were tested in order to identify their use as secondary material which is excellent in function and taste as food. Samples were soaked for 1 and 5 min with NaCl concentration(0, 1 and 3%) at a certain heating temperature(25, 75 and 95$\^{C}$), and then tannin, vitamin C, flavonol, color intensity, sensory test and textural properties were analysed. The results of the analyses were as follows. Tannins were decreased as heating temperature, NaCl concentration and soaking time were increased, especially, that the control was 420 mg% but decreased 228 and 198 mg% at 95$\^{C}$(1 and 3% NaCl concentration) for 5 min. soaked in each. Vitamin C content also decreased more in higher temperature and NaCl concentration than control(122.4 mg%). Color intensity showed higher value in 1. and b than in heating temperature, NaCl concentration and soaked time longer remarkably, but a value decreased. The peel of sweet persimmons was analyzed myricetin(2.0 $\mu$g/g), quercetin(34.5 $\mu$g/g) and kaemperol(1.1$\mu$g/g), but in pre-treatment sample(95$\^{C}$, 1% NaCl concentration and 5 min. soaked) was showed higher myricetin(9.5 $\mu$g/g) and quercetin(5.5 $\mu$g/g). Textural properties were good in pre-treatment sample(95$\^{C}$, 1% NaCl concentration and 5 min. soaked) such as brittleness, cohesiveness, gumminess and chewiness. In sensory analysis, the pre-treatment samples(95$\^{C}$, 1% NaCl concentration and 5 min. soaked and 95$\^{C}$, 3% NaCl concentration and 1 min. soaked) were showed higher point than others.

Establishment of Analytical Method for Methylmercury in Fish by Using HPLC-ICP/MS (고성능액체크로마토그래피-유도결합플라즈마 질량분석기를 이용한 어류 중 메틸수은 분석법 확립)

  • Yoo, Kyung-Yoal;Bahn, Kyeong-Nyeo;Kim, Eun-Jung;Kim, Yang-Sun;Myung, Jyong-Eun;Yoon, Hae-Seong;Kim, Mee-Hye
    • Korean Journal of Environmental Agriculture
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    • v.30 no.3
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    • pp.288-294
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    • 2011
  • BACKGROUND: Methylmercury is analyzed by HPLC-ICP/MS because of the simplicity for sample preparation and interference. However, most of the pre-treatment methods for methylmercury need a further pH adjustment of the extracted solution and removal of organic matter for HPLC. The purpose of this study was to establish a rapid and accurate analytical method for determination of methylmercury in fish by using HPLC-ICP/MS. METHOD AND RESULTS: We conducted an experiment for pre-treatment and instrument conditions and analytical method verification. Pre-treatment condition was established with aqueous 1% L-cysteine HCl and heated at $60^{\circ}C$ in microwave for 20 min. Methylmercury in $50{\mu}L$ of filtered extract was separated by a C18 column and aqueous 0.1% L-cysteine HCl + 0.1% L-cysteine mobile phase at $25^{\circ}C$. The presence of cysteine in mobile phase and sample solution was essential to eliminate adsorption, peak tailing and memory effect problems. Correlation coefficient($r^2$) for the linearity was 0.9998. The limits of detection and quantitation for this method were 0.15 and $0.45{\mu}g/kg$ respectively. CONCLUSION: Result for analytical method verification, accuracy and repeatability of the analytes were in good agreement with the certified reference materials values of methylmercury at a 95% confidence level. The advantage of the established method is that the extracted solution can be directly injected into the HPLC column without additional processes and the memory effect of mercury in the ICP-MS can be eliminated.

A study on the skeletal changes after treatment of Class III malocclusion patients (3급 부정교합 환자에서의 치료후 골격변화 양상에 관한 연구)

  • Chung, Dong-Hwa;Cha, Kyung-Suk
    • The korean journal of orthodontics
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    • v.26 no.3
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    • pp.267-279
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    • 1996
  • This study was investigated the changes during treatment and retention period in the Class III malocclusion patients and explored the correlationship between factors that showed relapse tendencies and pre-treatment skeletal pattern and the changes during treatment period. Numbers of total sample were 24 and their Hellman's dental age at the start of treatment was over III B and were retained at least over 1 year 6 months. The following conclusion were obtained by comparing the differences between treatment period and retention period, and after analysing the correlationship of factors that manifested relapse tendencies. 1. The angles formed by FH plane and occlusal plane, FH plane and mandibular plane, and mandibular incisor and mandibular plane changes showed rebound effect during retention period and among them occlusal plane angle and IMPA show reverse correlationship. 2. Upward displacement of the occlusal plane at the end of treatment has returning tendency, is proportional to the displacement during treatment period, but the angle between maxillary and mandibular 1st molar to its basal bone have been constantlsy maintained during the retention period. 3. Mandibular plane decrease during retention period and downward backward rotation during treatment period show correlationship.

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Establishment of Pre-Harvest Residue Limit (PHRL) of Insecticide Bifenthrin during Cultivation of Grape (포도의 재배기간 중 살충제 bifenthrin의 생산단계 농약잔류허용기준의 설정)

  • Kim, Sung-Woo;Lee, Eun-Mi;Lin, Yang;Park, Hee-Won;Lee, Hye-Ri;Riu, Myoung-Joo;Na, Ye-Rim;Noh, Jae-Eok;Keum, Young-Soo;Song, Hyuk-Hwan;Kim, Jeong-Han
    • The Korean Journal of Pesticide Science
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    • v.13 no.4
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    • pp.241-248
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    • 2009
  • Pre-Harvest Residue Limit (PHRL) of bifenthrin during cultivation of grape was established by utilizing the dissipation curve and biological half-life of bifenthrin calculated from the analysis of 0, 1, 2, 3, 5, 7, 10, 15 days after treatment of bifenthrin. Grape sample was extracted and partitioned with acetonitrile and dichloromethane, respectively, and bifenthrin was determined with GC/ECD. Limit of quantitation (LOQ) of bifenthrin was 0.01 ng. Recoveries at two fortification levels of 0.1 and $0.5\;mg\;kg^{-1}$ were $104.08\;{\pm}\;1.24$ and $92.25\;{\pm}\;3.13%$, respectively. The biological half-lives of bifenthrin were about 21 days at standard application rate, while, 23 days at double application rate. Dissipation of bifenthrin on grape was not influenced by growth dilution effect. The PHRLs of bifenthrin were recommended as 0.60 and $0.55\;mg\;kg^{-1}$ for 10 and 5 days before harvest, respectively.

Comparison of the Medication Effects between Milnacipran and Pregabalin in Fibromyalgia Syndrome Using a Functional MRI: a Follow-up Study (섬유근통 환자에 대한 Milnacipran과 Pregabalin 약물치료에 대한 기능적 자기공명영상에서의 후속 영향 비교)

  • Kang, Min Jae;Mun, Chi-Woong;Lee, Young Ho;Kim, Seong-Ho
    • Investigative Magnetic Resonance Imaging
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    • v.18 no.4
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    • pp.341-351
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    • 2014
  • Purpose : In this study, the medication effects of Milnacipran and Pregabalin, as well known as fibromyalgia treatment medicine, in fibromyalgia syndrome patients were compared through the change of BOLD signal in pain related functional MRI. Materials and Methods: Twenty fibromyalgia syndrome patients were enrolled in this study and they were separated into two groups according to the treatment medicine: 10 Milnacipran (MLN) treatment group and 7 Pregabalin (PGB) treatment group. For accurate diagnosis, all patients underwent several clinical tests. Pre-treated and post-treated fMRI image with block-designed pressure-pain stimulation for each group were obtained to conduct the statistical analysis of paired t-test and two sample t-test. All statistical significant level was less than 0.05. Results: In clinical tests, the clinical scores of the two groups were not significantly different at pre-treatment stage. But, PGB treatment group had lower Widespread Pain Index (WPI) and Brief Fatigue Inventory (BFI) score than those of MLN treatment group at post-treatment stage. In functional image analysis, BOLD signal of PGB treatment group was higher BOLD signal at several regions including anterior cingulate and insula than MLN treatment group at post-treatment stage. Also, paired t-test values of the BOLD signal in MLN group decreased in several regions including insula and thalamus as known as 'pain network'. In contrast, size and number of regions in which the BOLD signal decreased in PGB treatment group were smaller than those of MLN treatment group. Conclusion: This study showed that MLN group and PGB group have different medication effects. It is not surprising that MLN and PGB have not the same therapeutic effects since these two drugs have different medicinal mechanisms such as antidepressants and anti-seizure medication, respectively, and different detailed target of fibromyalgia syndrome treatment. Therefore, it is difficult to say which medicine will work better in this study.

Improvement of accuracy in quantitative TXRF analysis of soil sample by applying external standard method (외부표준법을 적용한 토양시료의TXRF 정량분석 정확도 개선)

  • Park, Jinkyu;Park, Ranhee;Han, Sun Ho;Lim, Sang Ho;Lee, Chi Gyu;Song, Kyuseok
    • Analytical Science and Technology
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    • v.29 no.6
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    • pp.261-268
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    • 2016
  • TXRF is a powerful technique for the soil sample analysis due to its ability to conduct quantitative analysis of powder sample without complicated pre-treatment processes. The conventional internal standard method used for this technique suffers from relatively low accuracy because of varying matrix effects of soil. In order to improve the accuracy, external standard method was applied to analyze two types of soil samples; acid-dissolutionized soil solution and detergent-suspended soil powder. Individual ICP-AES/MS grade standards were mixed, diluted and measured to create standard curves, but applying these curves for analyzing the soil solution sample did not make any improvement in comparison with the internal standard method. On the other hand, standard curves were created with using standard soil powders for the analysis of soil powder samples, and we found that this method increased the accuracy significantly relative to the internal standard method. Especially, Al, Fe, K, Ca, Ti, Ba, Mn, Sr, Rb, Cu was measured with relatively high accuracy (relative error = ${\pm}20%$).

Comparison of treatment effects between the modified C-palatal plate and cervical pull headgear for total arch distalization in adults

  • Park, Chong Ook;Sa'aed, Noor Laith;Bayome, Mohamed;Park, Jae Hyun;Kook, Yoon-Ah;Park, Young-Seok;Han, Seong Ho
    • The korean journal of orthodontics
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    • v.47 no.6
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    • pp.375-383
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    • 2017
  • Objective: The purpose of this study was to evaluate the dental and skeletal effects of the modified C-palatal plate (MCPP) for total arch distalization in adult patients with Class II malocclusion and compare the findings with those of cervical pull headgear. Methods: The study sample consisted of the lateral cephalograms of 44 adult patients with Class II Division 1 malocclusion, including 22 who received treatment with MCPP (age, $24.7{\pm}7.7years$) and 22 who received treatment with cervical pull headgear (age, $23.0{\pm}7.7years$). Pre- (T1) and post-treatment (T2) cephalograms were analyzed for 24 linear and angular measurements. Multivariate analysis of variance was performed to evaluate the changes after treatment in each group and differences in treatment effects between the two groups. Results: The mean amount of distalization at the crown and root levels of the maxillary first molar and the amount of distal tipping was 4.2 mm, 3.5 mm, and $3.9^{\circ}$ in the MCPP group, and 2.3 mm, 0.6 mm, and $8.6^{\circ}$ in the headgear group, respectively. In addition, intrusion by 2.5 mm was observed in the MCPP group. In both groups, the distal movement of the upper lip and the increase in the nasolabial angle were statistically significant (p < 0.001). However, none of the skeletal and soft tissue variables exhibited significant differences between the two groups. Conclusions: The results of this study suggest that MCPP is an effective treatment modality for total arch distalization in adults.

Application of ELISA for the Detection of Oxytetracycline Residue in Live Animals

  • Lee, H.J.;Lee, M.H.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.12
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    • pp.1775-1778
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    • 2000
  • Oxytetracycline has been widely used in the cattle industry to control pneumonia, shipping fever, foot rot, bacterial enteritis, and uterine infections. Extensive use of antibiotics in veterinary clinics has resulted in residues in tissue and bacterial resistance to antibiotics. To prevent unwanted drug residues from entering the human food chain, extensive control measures have been established by both government authorities and industries. The demands for reliable, simple, sensitive, rapid and low-cost methods for residue analysis of foods are increasing. In this study, we established a rapid test for tissue residues of oxytetracycline in cattle. The recommended therapeutic dose of oxytetracycline (withdrawal time, 14 days) was administered to 10 cattle. Blood samples were collected from each cow before drug administration and during the withdrawal period. The concentration of oxytetracycline in plasma, determined by a semi-quantitative ELISA, was compared to that of the internal standard, 10 ppb. The absorbance ratio of internal standard to sample (B/Bs) was employed as an index to determine whether the residues in cattle tissues were negative or positive. That is, a B/Bs ratio less than 1 was considered as residue positive and that greater than 1 as negative. Based on this criterion, all plasma samples from cattle were negative to oxytetracycline at pre-treatment. Oxytetracycline could be detected in the plasma treated cattle until day 14 post-treatment. The present study showed that the semi-quantitative ELISA could be easily adapted in predicting tissue residues for oxytetracycline in live cattle.

Chuna Manual Therapy for Stroke: A Systematic Review and Meta-analysis (뇌졸중의 추나요법에 대한 체계적 문헌 고찰)

  • Kim, Mi-Kyung;Han, Chang-Ho
    • The Journal of Churna Manual Medicine for Spine and Nerves
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    • v.14 no.2
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    • pp.15-28
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    • 2019
  • Objectives : This study aimed to systematically and comprehensively review controlled clinical trials on the effectiveness and safety of Chuna Manual Therapy for stroke. Methods : By October 7, 2019, three core databases and three domestic databases were searched. Seven major academic journals on the related field were also hand-searched. Methodological quality of the included studies was evaluated using the Cochrane risk of bias tool. Meta-analysis was conducted and the quality of its evidence was assessed using the GRADE methodology. Results : Five articles met the eligibility criteria. The results of most of included studies were in favor of Chuna Manual Therapy. No study described any adverse events during or after the clinical trials. Meta-analysis of three eligible studies showed that the pre- and post-treatment scores on the Modified Barthel Index (mean difference 8.00, 95% confidence interval 0.26 to 15.74) and Berg Balance Scale (mean difference 3.57, 95% confidence interval 0.64 to 6.51) of the Chuna-treated group were significantly different, but only marginally higher than those of the non-treatment group. Thus, the level of evidence gathered from these studies was assessed to be low. Conclusions : Based on current available evidence, any confirmative conclusions cannot be made on the effectiveness and safety of Chuna Manual Therapy for stroke because of the small sample size, low methodological quality, presence of statistical heterogeneity, and missing safety information. More rigorously designed large-scale multi-center studies are needed to establish more specific and credible evidence to support or oppose the use of Chuna Manual Therapy for stroke.