• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.46-46
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• 2003

Salviae Miltiorrhizae Radix has been used for treatment of cardiovascular diseases in oriental medicine. To investigate the possible involvement of cardiac ion channel in this effect, we examined electrophysiological effects of the extract of Salviae Miltiorrhizae Radix on action potentials and ionic currents in rat ventricular myocytes. The extracts of Salviae Miltiorrhizae Radix were fractionated into nine fractions, and the effect of each fraction on action potential was tested. The fraction containing monomethyl lithospermic acid-A (LSA-A) induced a significant prolongation of action potential duration (APD). LSA-B which is a major component of Salviae Miltiorrhizae Radix, however, did not cause a significant effect. In voltage clamp experiments, the effects of LSA-A on K currents, Ca currents and Na currents were tested. Neither K currents nor L-type Ca currents were affected by LSA-A. On the contrary, LSA-A significantly slowed down the inactivation kinetics of the Na current with no effect on the fast component of the inactivation process. The amplitude of the peak current and the voltage-dependence of activation were not changed by LSA-A. The effect of LSA-A on Na current was abolished when high concentration of $Ca^{2+}$ buffer (10 mM BAPTA) was included in the pipette solution or when Ca2+ current was blocked by nicardipine (1 $\mu$M) in the bath solution.n.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.21 no.3
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• pp.52-68
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• 2008

Objectives and Methods : Salviae miltiorrhizae Radix (SMR) promotes blood circulation to remove blood stasis, cools the blood to relieve carbuncle, clears away heat from the heart and tranquilizes the mind. This study was designed to investigate the effects of SMR on immuno-potentiative action in terms of changes in the genetic profile of dendritic cells (DC) using by microarray analysis. Results and Conclusion: In this experiment, treatments with more than 250 ${\mu}g/ml$ upto 1000 ${\mu}g/ml$ of SMR elevated the proliferation rates of DC. Microscopic observations confirmed the tendency on proliferation rates. Expression levels of genes related with cellular methabolic process, cell communication, and macromolecule metabolic process were elevated by treatment with SMR in comparison of functional distribution in a Biological Process. In molecular functions, expression levels of genes related with receptor activation, nucleotide binding and nucleic acid binding were elevated. In cellular components, expression levels of genes related to cellular membrane-bound organelles were elevated. In addition, expression levels of genes related to Wnt signalling pathways and the glycerophospholipid metabolism were elevated through analysis using pathway analysis between up-and down-regulated genes in cells treated with SMR. Finally, genes related to JAK2, GRB2, CDC42, SMAD4, B2M, FOS and ESRI located the center of Protein interaction network of genes through treatment with SMR.

• Korean Journal of Pharmacognosy
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• v.40 no.3
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• pp.184-189
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• 2009

A simple and reliable reverse phase HPLC method of Coptidis Rhizoma and Salviae Miltiorrhizae Radix were developed to determine pharmacologically active marker compounds. The water-extract of two natural medicines were evaluated by the HPLC methods. The stability test for two extract were examined for five months. However, no significant change in the content of the marker compounds of each extract observed during the time of investigation.

• Herbal Formula Science
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• v.22 no.2
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• pp.35-43
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• 2014

Objectives : The purpose of this study was to identify antiproliferative effects of Salviae miltiorrhizae Radix(SM) extracts against cancer cell lines. Methods : We used 2 kinds of cancer cell lines such as colon cancer cells(HT-29), human oral epitheloid carcinoma cells(KB). MTT assay was performed to examine the efficacy of SM extracts on the cytostaticity of cancer cells in proportion to time and doses. Apoptosis was evaluated by DNA laddering and DAPI nuclei staining. Results : The MTT absorbances against HT-29 and KB of SM extracts were significantly decresed. DNA ladders could be identified in KB of SM extracts. The morphological change were observed and number of cells were decreased by SM extracts. Conclusions : SM extracts is considered to be effective to induce apoptosis and inhibit cancer cell proliferation.

• Journal of the Korean Institute of Oriental Medical Informatics
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• v.11 no.2
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• pp.1-13
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• 2005

Objectives To research oriental medical treatments for diabetic nephropathy by literature study Methods We reviewed oriental medical book concerning the diabetic nephropathy. Results & Conclusions 1. The diabetic nephropathy has relation to edema, dizziness, exhaustion of strenght, obstruction and rejection among the transformations of emaciation and thirst. 2. The causes of diabetic nephropathy are dry heat caused congenital defect, mental depression and greasy diet damages liquid nutrients and thereafter Gi and Yang deficiency produces blood stagnation, dampness and phlegm-turbidity. 3. The medical treatments are invigorating Gi, promoting blood flow to remove blood stasis and removing dampness through diuresis. The frequently used herb are Radix Astragali, Poria, Fructus Corni, Rhizoma Dioscoreae, Radix Salviae Miltiorrhizae, etc. and Dansam is meanigfull of all herbs for invigorating blood and dispelling blood stagnation.

• Korean Journal of Pharmacognosy
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• v.22 no.2
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• pp.95-100
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• 1991

Salviae miltiorrhizae(SM) Radix extract increased $[^3H]-thymidine$ incorporation into rat hepatocytes at the concentration ranging from $5{\times}10^{-5}\;to\;5{\times}10^{-1}mg/ml$. It decreased the activities of s-GOT and s-GPT in cirrhotic rats induced by $CCl_4$, TAA and D-GalN, respectively and reduced the sleeping time induced by hexobarbital in $CCl_4$,TAA and D-GalN intoxicated mice, respectively. SM extract shortened the half-life of sulfobromophthalein in $CCl_4$ intoxicated rats.

• Journal of the Korean Society of Food Science and Nutrition
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• v.24 no.3
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• pp.459-465
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• 1995

The ether extract of Salviae miltiorrhizae Radix(SMR) was fractioned to give five subfractions, so that two subfractions of them were recrystallized to yield each pure diterpene quinone pigment. On the basis of spectral evidence, these two compounds were identified as tanshinone II and crytotanshinone. Cryptotanshinone exhibited both of a potent platelet anti-aggregating activity in vitro and a potent antimicrobial activity. GC-MS analysis of the other extract showed that tanshinone II was contained in the largest proportion of all the diterpene quinones. In addition, GC-MS analysis gave other valuable analytical informations.

• The Korea Journal of Herbology
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• v.22 no.4
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• pp.239-245
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• 2007

Objective : Salviae miltiorrhizae Radix (SMR) has widely used to treat patients with cardiovascular diseases such as coronary arteriosclerosis, angina pectoris and hyperlipidemia. This study was designed to investigate the effects of SMR on changes in serum cholesterol and protective effects on liver tissue damage in Hyperlipidemic rats. Methods : The present author investigated changes in serum glucose, cholesterols, AST/ALT and histopathological changes of liver tissue by oral administration of SMR in Rats. Results : In this study, body weights of hyperlipidemic rats induced high fat diet did not changed, and treatment with SMR did not affect body weights in hyperlipidemic rats. For experimental period, Food and Water uptake in SMR administered group were the same as those in hyperlipidemic control group. In this experiment, treatment with SMR decreased total cholesterol and AST in serum which elevated by high fat diet respectively. In addition, SMR administration protected liver tissue from damage induced by induction of hyperlipidemia. Conclusions: These results suggest that SMR is useful to treat patients with disease related to cardiovascular diseases including hyperlipidemia, because SMR can decrease cholesterol and AST in serum and also have non-specific protective effect on tissues including liver.

• The Korea Journal of Herbology
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• v.22 no.4
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• pp.65-73
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• 2007

Objective : Salvia miltiorrhiza Bunge (Labiatae) (SM), an eminent herbal plant, has been widely used in traditional Chinese medicine for the treatment of vascular diseases such as hypertension. The aim of this study was to determine whether SM inhibits production of nitrite, an index of NO, and proinflammatory cytokines in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. And this study investigated whether or not SM could reduce tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$)-induced inflammatory response in human vascular aortic smooth muscle cells (HASMC) and umbilical vein endothelial cells (HUVEC). Methods : Cytotoxic activity of SM on RAW 264.7 cells was using 5-(3-caroboxymeth-oxy phenyJ)-2H-tetra-zolium inner salt (MTS) assay. We measured the NO production using Griess Reagent System. Production of Proliflammatory cytokines was measured by Enzyme-Linked Immunosorbent Assay (ELISA). Results : Our results indicated that SM significantly inhibited the LPS-induced NO production accompanied by an attenuation of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), IL-6 and monocyte chemoattractant protein (MCP)-1 formation in macrophages. SM decreased TNF-${\alpha}$-induced IL-8, IL-6 production, and intracellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 expression. Conclusion : These results indicate that SM has potential as an anti-inflammatory agent.

• The Journal of Internal Korean Medicine
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• v.39 no.4
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• pp.480-494
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• 2018

Objectives: To investigate the effect of fermented extract of Artemisiae Iwayomogii Herba, Curcumae Longae, Crataegi Fructus and Salviae Miltiorrhizae Radix (FMH) on anti-inflammation associated with dyslipidemia and anti-oxidation in RAW264.7 and HUVEC cells. Methods: The total polyphenols, total flavonoids, DPPH radical scavenging activity, ABTS radical scavenging activity, and cytotoxicity of FMH were measured. RAW264.7 cells treated with FMH were tested for production of NO, and for cytokine and LTB4 levels and HUVEC cells treated with FMH were examined for production of cDNA of genes related to inflammation. Results: 1. FMH contained polyphenols and flavonoids. The DPPH and ABTS radical scavenging activity of FMH increased in a concentration-dependent manner. 2. FMH treatment inhibited the production of nitric oxide (NO), cytokines, and LTB4 in RAW264.7 cell when compared to the untreated control group. 3. FMH decreased the transcription of pro-inflammatory genes, whereas it increased transcription of anti-inflammatory genes, in HUVEC cells. Conclusion: FMH is effective as an antioxidant and for treatment and prevention of dyslipidemia, atherosclerosis, ischemic heart disease, stroke, and other cardiocerebrovascular diseases.