• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.5
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• pp.391-397
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• 1993

Rye(Secale cereale cv. Chunchu), barley(Hordeum vulgare cv. Dusan 29#), and italian ryegrass(Lolium multiflorum cv. Barmultra) were treated at 0, 0.1, 0.3, and 0.6 % of NaCl solution for 30 days. Plant height, leaf length, and leaf area were decreased with increasing NaCl level, and leaf area was decreased mainly due to decrease of leaf length. Root length was decreased at higher NaCl levels, but root number was increased. Shoot dry weight was decreased by increasing NaCl levels in rye and italian ryegrass, but barley did not show significant change. Dry weight of root was more decreased than that of shoot at higher NaCl levels. Chlorophyll content was decreased, but electric contuctivty of shoots and roots was increased at higher NaCl levels. Na+ content in shoot and root was increased with increasing NaCl level but the degree was not same. Reducing power of roots was that three crops as affected by NaCl was not equal. Barley was the most tolerant to NaCl stress.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.617-625
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• 2013

Stauntonia hexaphylla and Raphiolepis indica, cold-tolerant broadleaved evergreens ranging through the southern region of South Korea, were assessed on salt tolerance with NaCl treatment using visual damage, chlorophyll florescence image, and malondialdehyde (MDA) analysis. As NaCl concentrations increased, the soil pH decreased and EC increased, and the soil of S. hexaphylla was affected more strongly by the treatment than that of R. indica. In visual damage, S. hexaphylla withered above 200 mM NaCl at 20 days after the treatment. All individuals of R. indica survived during the experiment though the leaves of R. indica showed visual damages up to 400 mM NaCl. The color changes in chlorophyll fluorescence showed a strong correlation with the degree of visual damage. As NaCl increased, the red color of the leaves of S. hexaphylla was distinctly changed to blue and chlorophyll fluorescence decreased starting from the margin to the middle of a leaf. R. indica showed subtle color changes and remained in red color during the experiment. At five days after the NaCl treatment, the MDA of S. hexaphylla was above $4.56nmol{\cdot}g^{-1}$ when plants showed the highest visual damage and EC. The MDA of R. indica in all treatments showed below $1.5nmol{\cdot}g^{-1}$ except 400 mM NaCl treatment during the experiment.

• Journal of Korean Society of Forest Science
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• v.104 no.4
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• pp.564-577
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• 2015

The objectives of this study were to investigate the possibility of establishing a tree nursery at Saemangeum reclaimed land and to classify landscape trees based on the salt tolerance. A tree nursery (2.0 ha) was made in Gunsan Okgu area in 2012 with underground drain lines on the reclaimed land established in 2010. Salt content of the nursery soil within the 60 cm depth in 2013 was 5.13 dS/m and 8.20 dS/m for the pre-desalinated and non-desalinated lands, respectively. Thirty-six woody plant species (22 tree species and 14 shrub species at ages of 1 to 4) with a total of 3,943 individuals were planted in early April, 2013 and their growth performance was monitored until September of the same year. The average survival rate of the transplanted plants was 71.4% in late September, suggesting the high possibility of establishing a tree nursery at the reclaimed land. Based on the survival rate and tree vigor (amount of healthy leaves and crown development), the following 17 species with some salt tolerance were classified into three groups: "salt tolerant group" (3 species, Tamarix chinensis, Cudrania tricuspidata, Ilex serrata), "recommended group" (5 species, Pinus thunbergii, Albizia julibrissin, Ligustrum obtusifolium, Rosa rugosa, Pleioblatus pygmaeus), "plantable group" (9 species, Zelkova serrata, Hibiscus syriacus, Elaeagnus umbellata, Sorbus alnifolia, Sophora japonica, Metasequoia glyptostroboides, Quercus acutissima, Ulmus parvifolia, Robinia pseudoacacia). Seven tree species that had been adapted to the reclaimed land for three to four years before being transplanted to new reclaimed land in Gunsan Okgu area showed average survival rate of 98%, suggesting that pre-conditioned trees would survive well in the reclaimed land.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.161-161
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• 2017

Seed endophytes are very remarkable groups of bacteria for their unique abilities of being vertically transmitted and conserved. As plants attain hybrid vigor and heterosis in the process of crossbreeding, this might also lead to the changes in the community structure and diversity of plant endophytes in the hybrid plants ultimately affecting the endophytes of the seeds. It would be interesting to characterize how seed endophyte composition change over time. The objective of this study is to gain insights into the influence of natural crossbreeding and parental lineage in the seed bacterial endophytic communities of two pure inbred lines exploring contributions of the two most important sources of plant endophytes - colonization from external sources and vertical transmission via seeds. Total genomic DNA was isolated from rice seeds and bacterial DNA was selectively amplified by PCR. The diversity of endophytic bacteria was studied through Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis. Diversity between the original parents and the pure inbred line may show significant differences in terms of richness, evenness and diversity indices. Heat maps reveal astonishing contributions of both or either parents (IR29 ${\times}$ Pokkali and AT401 ${\times}$ IR31868) in the shaping of the bacterial seed endophytes of the hybrid, FL478 and IC32, respectively. Most of the T-RFs of the subsequent pure inbred line could be traced to any or both of the parents. Comparison of common and genotype-specific T-RFs of parents and their offspring reveals that majority of the T-RFs are shared suggesting higher transmission of bacterial communities common to both parents. The parents influence the bacterial community of their offspring. Unique T-RFs of the offspring also suggest external sources of colonization particularly as the seeds are cultivated in different ecogeographical locations. This study showed that host parental lines contributed greatly in the shaping of bacterial seed endophytes of their offspring. It also revealed transmission and potential conservation of core seed bacterial endophytes that generally become the dominant microbiota in the succeeding generations of plant hosts.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.10
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• pp.687-693
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• 2013

Coastal sand dunes have been seriously damaged caused by the development thoughtless for the environment and coastal erosion and destruction due to artificial structures like coast roads and breakwater. Hereupon, in this study we made a library of rhizobacteria that have the plant growth-promoting ability for plant rhizosphere of indigenous plants inhabiting in a coastal sand dune as well as the strong tolerance to salt, and evaluated the plant growth-promoting ability of these strains. Furthermore, we evaluated the effect of rhizobacteria on the growth rate of saline tolerant plants in sandy soil; selected out the most useful micro-organism for the restoration of a damaged sand dune. The effect of inoculation of strains selected from the first experiment on the growth of Peucedanum japonicum and Arundo donaxes planted in a coastal sand dune was evaluated. As a result, Bacillus aerius MH1RS1 had plant growth promoting activities: indole acetic acid (IAA) production, siderophores and 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase) activity, and also had a salinity tolerance. Also, in case of Peucedanum japonicum, the length of stems and weights of roots were enhanced by the inoculation of B. aerius MH1RS1. Fresh weights of stems and weights of roots in experimental group were, in particular, increased by 25% comparing with the control group. For an Arundo donax in experimental group, plant length increased by 18%, and weight of roots by 20% which is significant.

• Journal of the Korea Organic Resources Recycling Association
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• v.9 no.1
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• pp.65-72
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• 2001

Four newly isolated bacteria from soil were used to manufacture microbial inoculum to compost food waste. The bacteria, GM103, V25, V31, and V35, were identified as Bacillus licheniformis, B. subtilis, B. stearothermophilius, and B, subtilis, respectively. The bacterial strains were efficient to degrade protein and starch and also able to inhibit the growth of plant pathogenic fungus Rhizopus stronifer. The GM103 showed distinct capability in degrading starch, but grow only aerobically. The other three bacterial strains. V25, V31, and V35, could grow both aerobically as well as anaerobically, in 10%(w/v) salt, at $50^{\circ}C$, and had good viability and survival rate in soil. These characteristics of the bacterial strains are very adquate in Korean food composting containing high concentration of salt, especially at home. By mixing the 4 bacterial culture broth with molasses, beet pulp, zeolite, The bacterial inoculum for food waste composting-BIOTOP-CLEAN-was made. The performance of food waste composting by the BIOTOP-CLEAN was compared with that by control(not treated) and HS(other demestic company's inoculum product for food waste composting). The maximum temperature of the food waste during the composting with the BIOTOP-CLEAN was $50^{\circ}C$, while those of the control and HS were $30^{\circ}C$ and $35^{\circ}C$, respectively. The BIOTOP-CLEAN gave the good smell and showed dark brown color, while the control gave bad smell and HS gave less bad smell. These indicates that the food waste composting by the BIOTOP-CLEAN had been well accomplished. The culture broth of V25, V31, V35 were sparyed to the plants of tomato, chinese cabbage, raddish, red pepper every month and the spraying the culture broth to these plant significantly improved the production yield of the crops, due to the control effect of the bacterial strains against the plant pathogens.

• BMB Reports
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• v.38 no.2
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• pp.218-224
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• 2005

Antioxidant enzymes are related to the resistance to various abiotic stresses including salinity. Barley is relatively tolerant to saline stress among crop plants, but little information is available on barley antioxidant enzymes under salinity stress. We investigated temporal and spatial responses of activities and isoform profiles of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), non-specific peroxidase (POX), and glutathione reductase (GR) to saline stress in barley seedlings treated with 200 mM NaCl for 0, 1, 2, 5 days, respectively. In the control plant, hydrogen peroxide content was about 2-fold higher in the root than in the shoot. Under saline stress, hydrogen peroxide content was decreased drastically by 70% at 2 d after NaCl treatment (DAT) in the root. In the leaf, however, the content was remained unchanged by 2 DAT and increased about 14 % at 5 DAT. In general, the activities of antioxidant enzymes were increased in the root and shoot under saline stress. But the increase was more significant and consistent in the root. The activities of SOD, CAT, APX, POX, and GR were increased significantly in the root within 1 DAT, and various elevated levels were maintained by 5 DAT. Among the antioxidant enzymes, CAT activity was increased the most drastically. The significant increase in the activities of SOD, CAT, APX, POX, and GR in the NaCl-stressed barley root was highly correlated with the increased expression of the constitutive isoforms as well as the induced ones. The hydrogen peroxide content in the root was most highly correlated with the CAT activity, indicating an increased role of CAT in hydrogen peroxide detoxification under salinity stress. In addition, the results suggest the significance of temporal and spatial regulation of each antioxidant isoform in determining the competence of the antioxidant capacity under saline stress.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.4
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• pp.413-419
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• 1995

This study was conducted to find out the suitable NaCl concentration and temperature for investigating the varietal difference of salinity tolerance in Italian ryegrass. Seeds of 20 cultivars including 9 diploid and 11 tetraploid were exposed to eight levels of NaCl concentration ranging from 0 to 350mM under three temperature levels of 15, 20 and $25^{\circ}C$. The NaCl concentration(C50%) inhibited germination of 50% of the viable seeds for the cultivars at 15$^{\circ}C$ was 354mM, and those at 2$0^{\circ}C$ and $25^{\circ}C$ were 342mM and 325mM, respectively. There were significant correlations among C50% at three levels of temperature, and correlation coefficient for C50% between 2$0^{\circ}C$ and 15$^{\circ}C$ was higher than that between $25^{\circ}C$ and 15$^{\circ}C$ or 2$0^{\circ}C$. C50% was positively correlated only with germination speed at 10 days after sowing when the plants were exposed to 350mM NaCl at 2$0^{\circ}C$. For the germination speed at 10 days after sowing in 350mM NaCl at 2$0^{\circ}C$, the cultivars used could be classified into three groups. Wase Aoba, Bettina, E. K -11, Tetrone, Lira Sand, Tetra Florum and Billiken belonged to the tolerant group, and Tuchi Was, Wase Yutaka, Sakura Wase, Magnolia, Limella and Delita were as the semi-susceptible group, and Atalja, Barmultra, Ajax, Liberta, LM-16, Elving and Wilo were as the susceptible group.

• Journal of Korean Society of Forest Science
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• v.102 no.3
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• pp.323-330
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• 2013

This study was conducted to know the effect of major physical-chemical characteristics of soil to be in the area of indigenous and naturalized plant in the sand dune of the western coastal area of Korea. The study was performed on the sand dunes distributed across Taean-gun and Buan-gun and the study period was from April to October in 2010. Sixty nine of $5m{\times}5m$ study plots were installed and the distribution of plants were investigated. We measured the soil characteristics including soil pH, organic matter, total nitrogen, available $P_2O_5$, soil cation exchange capacity, exchangeable cation, EC and NaCl. As a result, soil texture was classified as sand, in case of average pH, NaCl, and EC of soil to be in the area of indigenous herbaceous plant were 7.77, 0.03% and $0.52ds.m^{-1}$, indigenous woody plant of the mean pH, NaCl and EC were 7.31, 0.01%, $0.23ds.m^{-1}$. In case of naturalized herbaceous plant of the mean soil pH, NaCl and EC were 7.12, 0.01%, $0.29ds.m^{-1}$, naturalized woody plant of the mean soil pH, NaCl and EC were 7.34, 0.01%, $0.20ds.m^{-1}$ respectively. On average, naturalize plants showed in low salinity concentration than indigenous plants.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.s02
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• pp.45-65
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• 1989

The Korean peninsular having the complexity of the photography and variability of climate is located within passing area of a lots of typhoon occurring from the southern islands of Philippines. So, there are various patterns of wind and flooding damages in paddy field occuring by the strong wind and the heavy rain concentrated during the summer season of rice growing period in Korea. The wind damages to rice plants in Korea were mainly caused by saline wind, dry wind and strong wind when typhoon occurred. The saline wind damage having symptom of white head or dried leaves occurred by 1.1 to 17.2 mg of salt per dry weight stuck on the plant which was located at 2. 5km away from seashore of southern coastal area during the period(from 27th to 29th, August, 1986) of typhoon &Vera& accompanying 62-96％ of relative humidity, more than 6 m per second of wind velocity and 22.5 to 26.4$^{\circ}C$ of air temperature without rain. Most of the typhoons accompanying 4.0 to 8. 5m per second of wind and low humidity (lesp an 60％) with high temperature in the east coastal area and southen area of Korea. were changed to dry and hot wind by the foehn phenomenon. The dry wind damages with the symptom of the white head or the discolored brownish grain occurred at the rice heading stage. The strong wind caused the severe damages such as the broken leaves, cut-leaves and dried leaves before heading stage, lodging and shattering of grain at ripening stage mechanically during typhoon. To reduce the wind damages to rice plant, cultivation of resistant varieties to wind damages such as Sangpoongbyeo and Cheongcheongbyeo and the escape of heading stage during period of typhoon by accelerating of heading within 15th, August are effective. Though the flood disasters to rice plant such as earring away of field, burying of field, submerging and lodging damage are getting low by the construction of dam for multiple purpose and river bank, they are occasionally occurred by the regional heavy rain and water filled out in bank around the river. Paddy field were submerged for 2 to 4 days when typhoon and heavy rain occurred about the end of August. At this time, the rice plants that was in younger growing stage in the late transplanting field of southern area of Korea had the severe damages. Although panicles of rice plant which was in the meiotic growing stage and heading stage were died when flooded, they had 66％ of yield compensating ability by the upper tilling panicle produced from tiller with dead panicle in ordinary transplanting paddy field. It is effective for reduction of flooding damages to cultivate the resistant variety to flooding having the resistance to bacterial leaf blight, lodging and small brown planthopper simultaneously. Especially, Tongil type rice varieties are relatively resistant to flooding, compared to Japonica rice varieties. Tongil type rice varieties had high survivals, low elongation ability of leaf sheath and blade, high recovering ability by the high root activity and photosynthesis and high yield compensating ability by the upper tillering panicle when flooded. To minimize the flooding and wind damage to rice plants in future, following research have to be carried out; 1. Data analysis by telemetering and computerization of climate, actual conditions and growing diagnosis of crops damaged by disasters. 2. Development of tolerant varieties to poor natural conditions related to flooding and wind damages. 3. Improvement of the reasonable cropping system by introduction of other crops compensating the loss of the damaged rice. 4. Increament of utilization of rice plant which was damaged.