• Korean Journal of Food Science and Technology
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• v.45 no.5
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• pp.590-597
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• 2013

High voltage pulsed electric fields (PEF) treatment is a promising non-thermal processing technology that can replace or partially substitute for thermal processes. The aim of this research was to investigate the microbial inactivation mechanisms by PEF treatment in terms of physiological changes to Saccharomyces cerevisiae. PEF was applied at the electric field strength of 50 kV/cm, treatment time of 56 ${\mu}s$ and temperature of $40^{\circ}C$. The microbial cells treated with PEF showed loss of salt tolerance on the cell membrane and collapse of the relative pH gradient on in-out of cells. Cell death or injury resulted from the breakdown of homeostasis, decreased $H^+$-ATPase activity, and loss of glycolysis activity.

• Korean Journal of Agricultural and Forest Meteorology
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• v.5 no.3
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• pp.191-199
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• 2003

Optimum transplanting time for extremely early rice cultivation as an after-crop of fruit and vegetables under greenhouse conditions in the southern area was determined. Rice was transplanted on March 10, March 20, March 30, April 10 and April 20 far three years from 1998 to 2000. Meteorological computations for rice production were high for heading between early May and early July, but they were too low for heading between late July and early August. Especially the expected yield predicted with 35,000 spikelets, the average spikelets per $m^2$ for extremely early transplanting. Computation for heading between late July and early August was low by 106 kg/10a compared with that yield at heading during the same period in the field. As the transplanting date in extremely early rice cultivation was earlier) rice growth at early stages was more retarded by low temperature. Rice growth at heading stage recovered with high temperature, showing less difference for the transplanting date. Abnormal tillers occurred by 15.5∼22.2%. The contribution of 1,000 grain weight${\times}$ripened grain ratio to yield of the extremely early rice cultivation in the greenhouse was 50.6%, indicating 16% hi일or than the degree of panicle per $m^2$ on yield. The estimated optimum transplanting time on the basis of yield for the extremely early greenhouse rice cultivation ranged from March 19 to April 28, and the estimated critical transplanting date on the basis of accumulated effective temperature was March 12. Rice reduced the amount of NO$_3$-N by 97.1% and EC by 90.5% in greenhouse soil with continuous fruit/vegetables fer more than a 10-year period, and completely removed the root-knot nematodes.

• Current Research on Agriculture and Life Sciences
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• v.6
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• pp.137-143
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• 1988

We studied suitable dough formula for yeast-raised breadmaking using frozen dough prepared by straight no-time method, centering around breadmaking quality. The most suitable dough formula based on 1,000 g of wheat flours was as follows : compressed yeast ; 30 g, sucrose ; 50 g, salt ; 20 g, shortening ; 40 g, potassium bromate ; 75mg, L-ascorbic acid ; 200mg, yeast food ; 3 g, vital wheat gluten ; 30 g, calcium phosphate, monobasic ; 400mg, sodium stearoyl-2-lactylate ; 8 g, water ; 680 g. Breadmaking test employing this formula showed that gassing power and fermentation time were suitable and higher specific loaf volume was obtainable. By using much emulsifiers and dough conditioners, frozen injury of dough was controlable in spite of the addition of more content of water(68%) than that of water (62%) obtained from the farinograph data.

• Asian Journal of Turfgrass Science
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• v.17 no.2_3
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• pp.87-97
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• 2003

The growth characteristics of creeping bentgrass seeded in the seaside landfill golf course are as follows. 1. As a result of analysis on the chemical characteristics of soil mixture used for turfgrass ground in this experiment, soil pH was 7.9, which is somewhat higher than the creeping bentgrass reference value of pH 5.5∼6.5; T-N(%) was 0.02, which is proper for the reference value, and trace element too lacked. 2. The cultivar with the fastest water infiltration was Seaside II recorded as 226.2cm/sec, while that with the slowest water infiltration was Pennlinks recorded as 141.1 cm/hr which was too faster than the USGA water infiltration reference value of 15∼30cm/hr. For the surface hardness of turfgrass ground with different cultivars, no statistically significant variation was found between the Penncross grass and the Pennlinks recorded as 18.6mm and 19.1 mm, respectively. The soil penetration was the highest in Pennlinks recorded as 7.6kg/$\textrm{cm}^2$ and lowest in Penn A-1 as 6.1kg/$\textrm{cm}^2$. 3. As a result of evaluation on visual quality at the early stage of growth, Penncross showed the most excellent visual quality compared to the others. However, Penn A-1 showed the most excellent visual quality at a late stage of growth around Sep. 17, 2003, and it was also excellent in the evaluation of visual color. Seaside II showed higher density around the root and the longest root length and was highly resistant to salt compared to others, but the initial sprouting rate was not satisfied, and the visual quality in the summer season was inferior to others. 4. As a result of measurement of the traffic injury, Penncross showed the most tolerant to the traffic stress and Pennlinks showed the most susceptible.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.1
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• pp.29-37
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• 2011

The experiment was carried out to find the effects of transplanting time and vinyl-film mulching treatment on the growth of artemisia by randomized block design with three replications. The experiment site ($100{\times}130$ m) was temporally established in the south-eastern part of Saemangeum reclaimed tidal land (near Gwanghwal myun, Gimjae-gun, Jellabukdo). Artemisia plants had been partly suffered from salt injury, because soil salinities in some area during growing period had been measured higher than 10 dS $m^{-1}$. Growth of plant height and survival ratio of transplanted plants had been significantly correlated with soil salinity and then the regression equations between plant height (y) and soil EC (x) and between survival ratio (y) and soil EC (x) were expressed as y=-16.59ln(x)+43.852 and $y=0.6453x^2-17.566x+103.99$, respectively. It was concluded that early transplanting and vinyl mulching was more beneficial for biomass production of artemisia, because biomass was 6.41 times more in the early transplanting than in the late transplanting, and 2.63 times more in the vinyl-film mulching than in the no mulching treatment.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2011.10a
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• pp.14-14
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• 2011

Polyamines (putrescine, spermidine and spermine) play pivotal roles in plant defense to different abiotic and biotic stresses. In order to understand the function of ginseng spermidine synthase gene, a key gene involved in biosynthesis of polyamines, transgenic plant was generated in Arabidopsis. The transgenic plants exhibited high levels of polyamines compared to the untransformed control plants. We investigated the tolerance capacity of transgenic plants to abiotic stresses such as salinity and copper stress. In addition, transgenic plants also showed increased resistance against one of the important fungal pathogens of ginseng, the wilt causing Fusarium oxysporum and one of important bacteria, bacterial blight causing Pseudomonas syringae. However, an activity of the polyamine catabolic enzyme, diamine oxidase (DAO) was increased significantly in F. oxysporum and P. syringae infected transgenic plant. Polyamine catabolic enzymes which may trigger the hypersensitive response (HR) by producing hydrogen peroxide ($H_2O_2$) seem act as an inducer of PR proteins, peroxidase and phenyl ammonium lyase activity. The transgenic plants also contained higher antioxidant enzyme activities, less MDA and $H_2O_2$ under salt and copper stress than the wild type, implying it suffered from less injury. These results strongly suggest an important role of spermidine as a signaling regulator in stress signaling pathways, leading to build-up of stress tolerance mechanisms.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.84-84
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• 2002

The advantages of the OPS techniques(Vajta G et al, Mol Reprod Dev 51: 53-58,1998) give 1) high survival rates of various types of eggs, 2) quick and simple process, 3) inexpensive equipment and reduced chilling injury. The efficiency of IVM/IVF technique in the porcine species is relatively lower than that obtained in other species such as ruminants. Two experiments were designed to investigate the effects of in-vitro fertilization of porcine oocytes matures using different OPS protocol for chilling and warming of vitrification. Porcine oocytes from ovaries collected at abattoir were matured for 44 hours in TCM199 Earle's salt supplemental with pyruvate, pff, L-cysteine, hormones and gentamycin. Oocytes were denuded and fertilized with frozen boar semen by common method. Porcine embryos produced routinely by in-vitro culture system of NCSU23 medium. The vitrification and the warming were conducted by OPS method with the glass micropipette instead of straw vessels and modified the protocol of G.Vajta(1999). In Exp 1, Chilling/Warming:Holding Medium(HM)+EG+DMSO/HM +sucrose Medium(SM) at 39$^{\circ}C$ warm stage. In Exp 2, : PBS+CS+EG+Ficoll+ Trehalose/PBS+Trehalose at 25$^{\circ}C$ stage. Filling, freezing, packing, thawing out and further culturing were performed to follow the basic protocol of G Vajta. During IVM-lVC and post-warming, fertilization parameter and developmental potential were compared to and statistically analysed. It was not significantly different from Exp 1 and Exp 2 but 25$^{\circ}C$ of stage was slightly higher on the morula/blastocyst forming rate and better atmosphere for worker than that at 39$^{\circ}C$ stage.

• Journal of Bio-Environment Control
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• v.13 no.3
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• pp.156-161
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• 2004

This study was conducted to investigate the effects of soil amendment with different characteristics on plant growth, fruit yield and quality of oriental melon for continuous cropping under protected cultivation. Humus layers in arable soil was disturbed because soil amendment from hillside to oriental melon field was continued to resolve problems for continuous cropping. Water potential and hardness of soil was decreased in sandy loam with lower clay contents compared with loam and silty clay. Leaf length and area, fresh and dry weight of plant at earlier growing stage were higher, but chlorophyll contents of leaves were dropped in sandy loam compared with silty clay soil. Fruit size and weight was higher in sandy loam, but soluble solid and color of fruit were increased in silty clay. Marketable and unmarketable yield and quantity of fermented fruit were the highest in sandy loam. Hardness and weight of fruit were decreased by longer storage period and soluble solids of fruit was peaked at 5 day after storage, but decreased by prolonged continued storage. Because of these results, soil characteristics of amendment to oriental melon field should be considered as an important factor for quality and yield of oriental melon.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.1
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• pp.15-20
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• 2009

Marine concrete structure is exposed to salt injury and deteriorated by steel corrosion due to chloride ions diffusion. It, therefore, is very important to estimate the chloride diffusivity in concrete. In this paper the compressive strength and permeable pore volume of concrete are measured and the diffusion coefficient and penetration depth of chloride ions in concrete were investigated to estimate the chloride diffusivity efficiently. To correlate these results each other, regression analysis was done. The results showed a good linear relation between chloride diffusivity and the fundamental properties of concrete and the chloride diffusivity of concrete with water-cement ratios of $40%{\sim}60%$ were about $2.5{\sim}6.6{\times}10^{-12}m^2/s$.

• Journal of dental hygiene science
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• v.19 no.3
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• pp.198-204
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• 2019

Background: Oxidative stress is a known to be associated with in the pathogenesis of many inflammatory diseases, including periodontitis. Ursolic acid is a pentacyclic triterpenoid with has antimicrobial, antioxidative, and anticancer properties. However, the role of ursolic acid in the regulating of osteogenesis remains undetermined. This study was aimed to elucidate the crucial osteogenic effects of ursolic acid and its ability to inhibit oxidative stress by targeting the immediate early response 3 (IER3)/nuclear factor erythroid 2-related factor 2 (Nrf2) pathway. Methods: Cell proliferation was determined using water-soluble tetrazolium salt assay, cell differentiation was evaluated by alkaline phosphatase (ALP) activity, and formation of calcium nodules was detected using alizarin red S stain. Generation of reactive oxygen species (ROS) was determined using by DCFH-DA fluorescence dye in hydrogen peroxide ($H_2O_2$)-treated MG-63 cells. Expression levels of IER3, Nrf2, and heme oxygenase-1 (HO-1) were analyzed using western blot analysis. Results: Our results showed that ursolic acid up-regulated the proliferation of osteoblasts without any cytotoxic effects, and promoted ALP activity and mineralization. $H_2O_2$-induced ROS generation was found to be significantly inhibited on treatment with ursolic acid. Furthermore, in $H_2O_2$-treated cells, the expression of the early response genes: IER3, Nrf2, and Nrf2-related phase II enzyme (HO-1) was enhanced in the presence of ursolic acid. Conclusion: The key findings of the present study elucidate the protective effects of ursolic acid against oxidative stress conditions in osteoblasts via the IER3/Nrf2 pathway. Thus, ursolic acid may be developed as a preventative and therapeutic agent for mineral homeostasis and inflammatory diseases caused due to oxidative injury.