• 한국식품영양학회지
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• 제21권1호
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• pp.15-21
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• 2008

In this study, the minimum inhibition concentration(MIC), growth inhibition activity, and colony forming inhibitory activity of water soluble propolis against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae and Salmonella enteritidis were tested. The MICs of the water soluble propolis against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, and Salmonella enteritidis were 312.5 ppm, below 156.3 ppm, 625 ppm, 10,000 ppm, above 10,000 ppm, 10,000 ppm, above 10,000 ppm, above 10,000 ppm, 10,000 ppm, and above 10,000 ppm, respectively. The growth inhibition concentrations against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, and Klebsiella pneumoniae were 156.3 ppm, below 156.3 ppm, 625 ppm, 5,000 ppm, 10,000 ppm, 10,000 ppm, 10,000 ppm, 10,000 ppm, and 5,000 ppm, respectively. However, 10,000 ppm did not inhibit the growth of Salmonella enteritidis. Finally, the colony forming inhibitory activities against Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, and Salmonella enteritidis were 98.0%, 99.8%, 69.8%, 98.1%, 62.0%, 63.1%, 79.5%, 61.9%, 79.6%, and 0.0%, respectively.

• 대한수의학회지
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• 제40권3호
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• pp.515-524
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• 2000

The present study was conducted to investigate the phage types(PT) of Salmonella enteritidis(SE) isolated from animals and human patients in Korea during the period 1993-1999. SE were isolated from chickens including the layer and broiler, duck, rat, swine and human patients in nationwide scale. A total of 231 non-human and 14 human SE strains were phage typed and 80.4%(197/245) of SE isolates tested could discriminate as a ten SEPTs including the RDNC by the 10 standard phages, which were brought from the Central Public Health Laboratory in England. In analysing of phage types both animal and human SE isolates, SEPT 4(55.4%) was the most prevalent in domestic animals including the layer(39.3%) and broiler(64.5%), duck(55.5%), and swine(75.0%) since 1993. About 57% of the S enteritidis from human was SEPT 4. Along with SEPT 4, SEPT 1, SEPT 6a, SEPT 7 and SEPT 7a var, SEPT 9b, SEPT 15, SEPT 21b and SEPT 22 were also found from animal and human SE isolates. SEPT 4(40.0%) was isolated from frozen chicken meats imported from China along with SEPT 7 and 7a var strains in this study. In conclusion, SEPT 4 was the most predominant SEPT in our country regardless of animal or human hosts including the Che-Ju island and almost exclusively found in domestic poultry(layer and broiler). During the review period, SEPT 4 has already been spreading continually among the animal hosts since 1993.

• 한국유기농업학회지
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• 제24권3호
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• pp.497-510
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• 2016

본 연구는 Lactobacillus bulgaricus SP5 분비물의 항균물질 탐색과 특성을 조사하고 병원성 미생물에 대한 억제효과를 얻고자 실시하였다. L. bulgaricus로부터 얻어진 상징액에 대한 항균활성은 시험에 이용된 모든 병원성 미생물인 E. coli 11234, Salmonella. enteritidis 3313, S. enteritidis 12021, 그리고 S. typhimurium 40253에 대해서 항균활성을 가지는 것으로 나타났다. 상징액 중의 황균활성 성분을 확인하기 위하여 다양한 pH 조정과 열처리 후, 병원성 미생물에 대한 항균활성을 측정하였다. 상징액의 열처리 후, 병원성미생물에 대한 항균활성은 비열처리와 같은 항균활성을 나타내었으나, 상징액의 pH를 4.6에서 중성이상으로의 변화는 항균활성이 현저히 감소하였다. 따라서 상징액의 항균활성은 pH 변화에 대해서 불안정하였으나, 열에 대해서는 매우 안정한 것으로 밝혀졌다. 이상의 결과를 통해 상징액의 병원성균에 대한 항균작용은 박테리오신 같은 peptide 보다는 lactic, acetic, phosphoric, succinic, pyroglutamic, citric, malic, 그리고 formic acid 등 다양한 유기산의 작용에 의한 것으로 확인되었다.

• 대한수의학회지
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• 제53권1호
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• pp.43-48
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• 2013

Commercial bivalent killed Salmonella vaccine Salenvac-T has been used in several countries in order to prevent salmonellosis with Salmonella enterica serovars Enteritidis (SE) and Typhimurium (ST) in poultry. However, this vaccine has not been used in poultry farms in South Korea. In this study, we evaluated the efficacy of Salenvac-T vaccine to protect against the challenge of virulent SE and ST, and the effect of the vaccine on egg production and mortality in layer hens. The colonization of liver, spleen and cecum with challenged SE and ST was reduced in vaccinated chickens compared with that of unvaccinated control group. The twice vaccination with Salenvac-T induced elevated antibody responses against both SE and ST detected by enzyme-linked immunosorbent assay (ELISA). The higher average hen-day production was observed in the vaccinated layer hens than in the unvaccinated layer hens without significance. The average mortality was lower in the vaccinated layer hens during the experiment period. The antibody responses to both SE and ST were persistently detected in the vaccinated layers. In summary, vaccination with Salenvac-T reduces colonization of internal organs and induces good antibody responses, thereby results in higher performance and lower egg contamination with SE and ST in layer hens.

• 생약학회지
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• 제36권3호통권142호
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• pp.252-256
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• 2005

The in visto inhibitory activities of essential oils of the Rosmarinus officinalis as well as its main constituents were evaluated against antibiotic-susceptible and -resistant strains of Staphylococcus aureus, streptococcus pneumoniae, Salmonella enteritidis and S. typhimurium. The essential oil fraction of R. officinalis and its main components, 1,8-cineole and camphor, exhibited significant inhibitory activities against most of the tested strains in this study, with MICs(minimum inhibitory concentrations) racing from 0.5mg/ml to 16mg/ml. The total oil fraction showed higher activity than its main components, 1,8-cineole and camphor against S. aureus strains. No remarkable differences were evident between MICs of the susceptible and resistant strains of S. aureus. Among the tested strains, S. pneumoniae CCARM 3523, the resistant strain to norfloxacin, oxacillin and erythromycin exhibited significantly lower sensitivity to the tested oils than antibiotic-susceptible strain. The oils revealed mostly higher inhibitory activity against S. typhimurium than against S. enteritidis.

• Molecular & Cellular Toxicology
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• 제5권4호
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• pp.323-327
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• 2009

• 대한미생물학회지
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• 제10권1호
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• pp.9-12
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• 1975

1974년도(年度) 1년간(年間) 각시도(各市道) 위생시험소(衛生試驗所)와 보건소(保健所) 등(等)에서 검사(檢査) 의뢰된 검체(檢體) 1504건중(件中)에서 717주(株)의 Salmonella 균속(菌屬)을 분리(分離) 동정(同定)하였다. 동정(同定)된 717주중(株中)에서 Salmonella Typhi가 698주(株)로 대부분을 차지하고 있었으며 그 외(外)의 Salmonella 균주(菌株)가 19주(株)이 었는데 이들을 seretype별(別)로 나누어 보면 다음과 같다. Salmonella Group A S. parathyphi A 7 주(株) Salmonella Group B S. paratyphi B 1 주(株) S. typhimurium 6 주(株) Salmonella Group C S. thompson 1 주(株) Salmonella Group D S. typhi 698 주(株) S. enteritidis 4 주(株) 항생물질(抗生物質)에 대(對)한 감수성시험(感受性試驗) 결과(結果) ampicillin에 대(對)한 감수성균(感愛性菌)이 99.6%이었고 chloramphenicol에 대(對)한 감수성균(感受性菌)이 93.4%이었으며 gentamycin에 대(對)하여서는 88.6%가 감수성균(感受性菌)이었고 streptomycin에 대(對)한 감수성균(感受性菌)이 49.7%이었으며 tetracycline에 대(對)하여서는 42.3%가 감수성균(感受性菌)이었다.

• 약학회지
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• 제54권2호
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• pp.122-125
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• 2010

The essential oil fraction was extracted from the aerial parts of the plant by steam distillation method and its composition was analyzed by GC-MS (gas chromatography-mass spectrometry) which led to the identification of 43 compounds. Dehydroelsholtzia ketone (56.81%) and elsholtzia ketone (30.05%) were identified as the predominant components of this oil. The antibacterial activities of the essential oil fraction were assessed by micro-dilution tests against antibioticsusceptible and -resistant strains of Streptococcus pneumoniae, Staphylococcus aureus, Salmonella enteritidis, and S. typhimurium. The oil inhibited most of the tested strains significantly resulting MICs (minimum inhibiting concentrations) between 2 mg/ml and >16 mg/ml. In most cases of this study Streptococcus pneumoniae and Staphylococcus aureus showed higher sensitivity to this oil than Salmonella strains.

• 한국식품영양학회지
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• 제14권6호
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• pp.579-584
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• 2001

Chitosna을 Bacillus pumilus BN-262 유래의 chitosanase로 처리하여 trimer, tetramer, pentamer가 전체 올리고당 중 64.3%에 달하는 비교적 저분자의 chitooligosaccharides로 구성된 LMW-chitooligosaccha-rides를 얻었으며, chitosan을 Trichoderma viride 유래의 cellulase로 처리하여 중합도7이상의 것이 전체 올리고당 중 49.3%에 달하는 상대적으로 분자량이 큰 chitooligosaccharides로 구성된 HMW-chitooligosac-charides를 얻었다. 제조된 chitooligosaccharides의 항균성과 콜로니 형성 저해 활성을 측정하였다. LMW chitooligosaccharides의 Bacillus cereus, Bacillus subtilis, Candida albicans, Escherichia coli, Escherichia coli O157:H7, Lactobacillus plantarum, Listeria mo-nocytogenes, Pseudomonas aeruginosa, Salmonella enteritidis, Salmonella typhimurium, Staphylococcus aureus, Streptococcus mutans에 대한 MIC(최소저해농도)는 각각 1.5%, 1.5%, above 2.0%, 1.5%, 1.5%, below 0.5%, 2.0%, 1.5%, above 2.0%. 1.0%, 1.5%, 1.0%였으며, HMW-chitooligosaccharides의 Bacillus cereus, Bacillus subtilis, Candida albicans, Esche-richia coli, Escherichia coli O157 : H7, Lactobacillus plantarum, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella enteritidis, Samlmonella typhimurium, Staphylococcus aureus, Streptococcus mutans에 대한 MIC는 각각 1.0%, 1.0%, 2.0% 이상, 1.0%, 1.0%, 0.25% 이하, 1.0%, 1.0%, 2.0%, 1.0%, 1.0%, 0.5%였다.

• 대한수의학회지
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• 제43권1호
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• pp.41-47
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• 2003

In this study, antibacterial activity on the chicken feed supplemented with different concentrations of oriental herbal medicine feed additives (OHMFA) extract was tested for some organisms and their preventive effects on the colonization of Salmonella enteritidis (SE) in broiler chickens were examined. The growth of Bacillus cereus, listeria monocytogenes and Staphylococcus aureus was inhibited on the feed of 0.25% OHMFA and Salmonella spp. and Escherichia coli O157:H7 were inhibited on the feed containing 2.0% level. The broiler chickens fed a forage additived 1.0% level of OHMFA after inoculation of a $10^7CFU/ml$ of SE were increased in weight than the chicken fed forage without OHMFA. The incidence of SE in liver, spleen and cecum of the chickens fed forage with OHMFA was decreased on the 7th days of postinfection and SE was not isolated from the organs of the chickens after the 14th days of postinfection. Serum antibody titers of the chickens were lowered than the control group.