• Journal of Oral Medicine and Pain
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• v.40 no.3
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• pp.124-129
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• 2015

Purpose: Salivary pH is an easily measurable biochemical marker and related to various intraoral and systemic conditions. The aim of this study was to evaluate the reliability of the salivary pH measurement using pH paper. In addition, the normal values of salivary pH using pH paper were compared to those of pH meter to investigate the validity. Methods: Twenty healthy male participants attended this study (mean age, $24.5{\pm}1.47$ years). Unstimulated saliva and stimulated saliva were collected from each subject two times with the interval of a day and salivary pH was immediately measured by the two experienced examiners using pH paper and pH meter. The salivary pH was compared between the groups and inter- and intra-examiner reliability of pH paper was investigated. The intraclass correlation coefficient (ICC) was used to calculate variations. Results: All measurements had good to excellent inter-examiner (ICC 0.755 for unstimulated; 0.760 for stimulated saliva), intra-examiner (ICC 0.635 for unstimulated; 0.592 for stimulated saliva) reliability and two measurement methods using pH paper and pH paper also showed high reliability (ICC 0.852 for unstimulated; 0.640 for stimulated saliva). The values measured by pH paper were significantly lower than those measured by pH meter. Conclusions: pH paper showed adequate inter- and intra-examiner reliability and it presented the validity in terms of comparison with the pH meter as a standard in the salivary pH measurement.

• Journal of the korean academy of Pediatric Dentistry
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• v.44 no.3
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• pp.298-305
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• 2017

This study aimed to compare chair-side test results for caries risk assessment and evaluate how well the tests reflect caries experience. The study was conducted on children aged < 6 years in primary dentition. Dental examination of children was conducted to determine the dmft index and subjects were divided into two groups : group I (dmft < 6), group II (dmft > 6). This study used four kinds of test kits (Plaque-check PH kit, Saliva-check buffer kit, Saliva-check mutans kit, Cytoperio analysis system). Saliva buffer capacity was significantly low in the high caries experience group (dmft > 6) and correlated with dmft index. Saliva pH level correlated significantly with saliva buffer capacity. The results showed that plaque pH and saliva pH levels had no correlation with dmft index. The Streptococcus mutans level measured by using the Saliva-check mutans and Cytoperio analysis system did not correlate with dmft index.

• Journal of Oral Medicine and Pain
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• v.17 no.2
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• pp.87-97
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• 1992

The purpose of this study is to investigate the age-and sex-related changes in the pH of resting saliva, viscosity, microorganisms and immunoglobulin A of stimulated whole saliva, and to investigate their correlations. The 120 healthy subjects were included in this study and the author used cone-and plate digital viscometer for viscosity, MSB agar for Streptococcus mutans, SL Rogosa agar for lactobacilli, and single radial immunodiffusion technique for immunoglobulinA. The obtained results were as follows : 1. There was no significant difference in pH, viscosity, Streptococcus mutans lactobacilli and immunoglobulin A of the saliva between males and females. 2. The viscosity values of stimulated whole saliva showed the increasing pattern with aging. 3. DMFS (or dmfs) rate was not correlated with pH, viscosity, Streptococcus mutans, lactobacilli and immunoglobulin A of the saliva. 4. There was a significant difference in the concentration of immunoglobulin A between the group under 10 and groups above 10. 5. The viscosity values of stimulated whole saliva showed the increasing pattern with decreasing of the number of Streptococcus mutans.

• Journal of dental hygiene science
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• v.6 no.3
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• pp.159-162
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• 2006

This study examined the relationship between the quantity of Streptococcus mutans and Lactobacillus spp. related to dental caries and the degree of acidity in saliva. A total of 240 saliva samples were taken from 80 subjects at the faculty of dentistry in Skopje, Macedonia. The saliva samples were taken by stimulating saliva production stimulation with paraffin chewing. However, no stimulation was applied when obtaining the samples used for measuring the pH. The data showed that in the caries group, S. mutans in 1 ml of saliva formed colonies with confluent growth (CFU > $10^6$ and $10^4-10^5$) in 100% of samples, whereas the Lactobacillus spp. formed colonies with confluent growth in 78.3%. In contrast, no colonies with confluent growth (CFU > $10^6$ and $10^5$) were found in the control group (with healthy intact teeth). In the caries group, the pH of the saliva was slightly acidic (pH = 5.90 - 6.50) and the buffering capacity was very low (below 0.7 ml of saliva per min). On the other hand, the pH of the saliva in the control group was neutral (pH 7.01 - 7.7) and the buffering capacity was high (over 1 ml of saliva per min). The increased number of S. mutans and Lactobacillus spp. in 1 ml of saliva (above $10^5$ CFU or more) from the CRT (Caries Risk Test, Vivadent, Liechtenstein) bacteria test can indicate an increased caries risk as well as slightly higher acidity of the saliva. Overall, these results reveal that the caries risk can be predicted by simply measuring the pH and buffering capacity of saliva, and can be used to monitor the effect of dental hygiene practices with the aim of preventing dental caries.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.22 no.4
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• pp.398-405
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• 2015

Purpose: This study was aimed to compare the effect between wet gauze with cold normal saline and wet gauze with cold water on thirst, oral cavity condition, and saliva pH among postoperative patients. Methods: This study was a quasi-experimental study with 56 participants. The experimental group received wet gauze with cold normal saline, and control group received wet gauze with cold water for three times at 15 minute intervals. Data were collected using visual analog scale for thirst, oral assessment guide for oral cavity condition and acidity tape for saliva pH. Measurement were made before applying the gauze and at 15min, 30min, and 45min after applying gauze. Data were analyzed using repeated measured ANOVA. Results: Thirst, oral cavity condition, and saliva pH improved in both groups with increasing number of gauze application. Thirst for the experimental group improved more than for the control group (F=4.29, p=.009), oral cavity condition except saliva, and saliva pH were not significantly different between the groups. Conclusion: This study results indicated that nurses can apply wet gauze with cold normal saline to reduce thirst and saliva acidity, and to improve the oral cavity condition for postoperative patients.

• Journal of Oral Medicine and Pain
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• v.34 no.3
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• pp.227-235
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• 2009

Many of the protective functions of saliva can be attributed to the biological, physical, structural, and rheological characteristics of salivary glycoproteins. Therefore, the development of ideal saliva substitutes requires understanding of the rheological as well as biological properties of human saliva. In the present study, we investigated the changes of salivary enzymatic activities by saliva substitutes and compared viscosity of saliva substitutes with human saliva. Five kinds of saliva substitutes such as Moi-Stir, Stoppers4, MouthKote, Saliva Orthana, and SNU were used. Lysozyme activity was determined by the turbidimetric method. Peroxidase activity was determined with an NbsSCN assay. $\alpha$-Amylase activity was determined using a chromogenic substrate, 2-chloro-p-nitrophenol linked with maltotriose. The pH values of saliva substitutes were measured and their viscosity values were measured with a cone-and-plate digital viscometer at six different shear rates. Various types of saliva substitutes affected the activities of salivary enzymes in different ways. Stoppers4 enhanced the enzymatic activities of hen egg-white lysozyme, bovine lactoperoxidase (bLP), and $\alpha$-amylase. Saliva Orthana and SNU inhibited bLP activity and enhanced $\alpha$-amylase activity. MouthKote inhibited $\alpha$-amylase activity. Moi-Stir inhibited the enzymatic activities of bLP and $\alpha$-amylase. The pH values were very different according to the types of saliva substitutes. Stoppers4, MouthKote, and Saliva Orthana showed lower values of viscosity at low shear rates and higher values of viscosity at high shear rates compared with unstimulated and stimulated whole saliva. Moi-Stir and SNU displayed much higher values of viscosity than those of natural whole saliva. Collectively, our results indicate that each saliva substitute has its own biological and rheological characteristics. Each saliva substitute affects the enzymatic activity of salivary enzyme and finally oral health in different ways.

• Journal of Korean society of Dental Hygiene
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• v.13 no.3
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• pp.509-515
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• 2013

Objectives : This study was performed to evaluate the salivary secretion, salivary pH and cariogenic activity using unstimulated whole saliva in patients with hematologic malignancy. Methods : Nineteen patients (9 male, 10 female) who had hematologic malignancy and were treated with chemotherapy or bone marrow transplantation, and nineteen normal volunteers (7 male, 12 female) as control group were included. The mean age of patients group and control group was 45.1 and 46.7 years, respectively. Patients group was examined salivary secretion, salivary pH, and cariogenic activity using unstimulated whole saliva and was compared with control group. Results : In comparison with control group, salivary secretion, salivary pH and salivary buffer capacity were significantly lower in patients with hematologic malignancy (p<0.01). Both cariogenic activity(p<0.01) and the number of Lactobacilli(p<0.05) are higher in patients group than control group. Conclusions : These results suggest that the unstimulated whole salivary secretion, pH and buffer capacity were lower in patients with hematologic malignancy than control group. Cariogenic activity is higher in patients with hematologic malignancy than control group. Such salivary factor and cariogenic activity can increase the possibility of induction of dental caries.

• Journal of Nutrition and Health
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• v.7 no.4
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• pp.21-23
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• 1974

Nitrite, one of the precursors of dimethylnitrosamine distributes widely in natural foods and also used as a color fixative in meat products. In this paper Nitrites and Nitrates contents in Korean-Kimchi and Human Saliva were as follows. (1) pH: $pH:\;3.8{\sim}5.8$(in Korean-Kimchi soup) (2) NaCl: $1.5{\sim}3.1%$(in Korean-Kimchi soup) (3) Acidity: $0.38{\sim}0.62%$(in Korean-Kimchi soup) (4) $NO_2-$ $0.25{\sim}0.68ppm$ (in Korean-Kimchi) $1.9{\sim}5.0ppm$ (in Human Saliva) (5) $NO_3-$ $35.0{\sim}92.0ppm$ (in Korean-Kimchi) $7.6{\sim}28.0ppm$ (in Human Saliva) These results showed low contents in Korean-Kimchi and Human Saliva.

• Journal of Oral Medicine and Pain
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• v.33 no.3
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• pp.241-246
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• 2008

Lichen planus is a common, chronic inflammatory disease of the skin and mucous membrane for which no precise causes have been confirmed. But it is often connected with infections. Helicobacter pylori(H. pylori) among various bacteria has been associated with the cause of gastritis, peptic ulcer and gastric cancer. Considering the similarities of histological features between gastric ulcer and oral ulcers, it is resonable to assume that H. pylori might also be involved in the development oral mucosal ulceration. So we employed this study to investigate the possible involvement of H. pylori in the aetiology of erosive oral lichen planus. We analyzed detection rate of H. pylori in saliva of patients with erosive oral lichen planus by nested PCR. As a result, it revealed a significant difference statistically by showing positivity in 16 to 21(76.2%) saliva samples of patients group and in 11 of 44(25%) saliva samples of control group(P>0.001). We were able to suppose that H. pylori in saliva can be related to cause of erosive oral lichen planus.

• Journal of Oral Medicine and Pain
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• v.46 no.4
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• pp.109-116
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• 2021

Purpose: Xerostomia is subjective feeling of dry mouth. It is complicated and multifactorial, which burdens clinicians in diagnosis and treatment of the problem. The goal of this study was to discuss the clinical importance of salivary flow rate, pH and subjective symptoms for evaluating oral dryness among young healthy male subjects. Methods: Thirty male participants were recruited in this study (mean age±standard deviation of 25.70±1.84). All participants completed 'Xerostomia Inventory' to measure subjective oral dryness scores. Unstimulated saliva and stimulated saliva were collected from each participant twice a day at 12:00 pm and 5:00 pm, using spitting method. Salivary flow rates and pH were measured immediately after collection. Relationship between objective and subjective measurements were analyzed. Results: There were excellent intra-examiner reliability for salivary flow rate and pH and good internal consistency for Xerostomia Inventory. Objective measurements and subjective symptoms did not exhibit positive association. Salivary flow rate in unstimulated and stimulated condition showed positive association and also for salivary pH. Stimulated salivary flow rate also presented positive correlation with stimulated salivary pH. Conclusions: Comprehensive assessment of objective measurements and subjective symptoms may be complimentary for assessing oral dryness, which would assist in implementing early interventions to improve patient's quality of life.