• 한국발생생물학회지:발생과생식
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• 제17권4호
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• pp.435-440
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• 2013

Previous studies, including our own, have demonstrated that the intratesticular injection of hypertonic saline (20%) decreased serum testosterone level which was similar to the surgical castration in the rat, showing the state of chemical castration. In the present study, we further verify the efficacy of this less invasive method as an alternative of surgical orchidectomy in the andrological field. Sterilized 20% saline was directly injected into the adult male rats (750 ${\mu}l$ per testis). The tested rats were divided into 3 groups including intact group (intact), orchidectomy group (ORX) and saline injection group (SAL) after bilateral orchidectomy was performed at the same day of injection. All rats were sacrificed at 4 weeks after injection. The reproductive organs (testes, epididymis, seminal vesicles and prostates) were collected and used for DNA and protein pattern analyses. Also, patho-histological studies on the testes were performed. In contrast to the intact group, similar DNA damages of testis and seminal vesicle were appeared in ORX group and SAL group. The DNA degradations seemed to be the results of necrosis rather than apoptosis. In the protein pattern analysis, all the testing tissues exerted similar patterns in the ORX group and the SAL group compared to the those of intact group. Patho-histological studies revealed that severe degenerative changes in testicular seminiferous tubules and massive infiltration of immune cells in SAL group. The present study confirmed that direct injection of hypertonic saline into the testis caused the equivalent biochemical changes in the accessory sex organs as shown in the orchidectomized animals. These results suggest that hypertonic saline injection model could be a useful castration model which can substitute for surgical castration when its safety is secured through further study in the future.

• 한국수자원학회논문집
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• 제48권1호
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• pp.23-35
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• 2015

본 연구에서는 3차원 수치모형을 이용하여 염수 대수층 내 담수 주입량에 따른 주입정과 양수정 사이의 담수체의 거동을 수치적으로 분석하였다. 대수층 내에 8개의 주입정이 동심원 상에 등간격으로 배열되어 있으며, 동심원 중심에 한 개의 양수정이 위치해 있다. 주입량이 상대적으로 작은 경우 주입정 주변의 담수체는 인근의 주입정에서의 담수체와 서로 혼합되지 않고 독립적으로 분포한다. 그러나 주입량이 증가함에 따라 담수체의 크기는 점차적으로 증가하며, 주입정과 주입정 사이에 염수와 담수가 혼합되어 있는 영역이 나타난다. 혼합되는 정도는 주입량이 증가함에 따라 증가되며, 이러한 현상은 양수정 주변에서도 동일하게 나타난다. 또한주입량이 증가할수록 양수정 주변 및 내에서의 염수비율이 점차적으로 감소하는 경향을 나타내었다.

• 한국발생생물학회지:발생과생식
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• 제14권4호
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• pp.281-286
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• 2010

최근 고장성(20%) 생리식염수를 흰쥐 정소에 직접 주사할 경우, 혈중 테스토스테론 수준이 최저 수준에 도달하는 화학적 거세 상태에 들어감이 보고되었다. 본 연구는 이러한 간단한 생리 식염수 주사 모델의 효용성을 더 검증하기 위해 정소의 미세 해부 구조상의 변화를 조사하였다. 실험군으로는 무처리군(intact, control), 정소제거군(orchidectomy), 그리고 식염수 주사군(saline-injection)으로 하였으며, 식염수 주사군에는 정소당 $750{\mu}{\ell}$의 멸균한 20% 식염수를 직접 주사하였다. 정소 제거 수술은 식염수 주사가 행해진 날에 시행하였다. 주사후 30일 경과 후에 동물을 희생하여 생식조직들을 채취하고 무게를 측정한 후 조직학적 조사를 위해 고정시켰다. 체중의 경우 무처리 대조군과 비교하여 정소절제군과 식염수 주사군 모두에서 유의한 변화는 없었다. 식염수 주사군의 정소 무게는 무처리 대조군에 비해 유의하게 감소하였다. 부정소와 저정낭의 무게의 경우 무처리 대조군에 비해 정소절제군과 식염수 주사군 모두에서 유의하게 감소하였으며, 전립선의 경우 무처리 대조군에 비해 정소절제군에서는 유의하게 감소하였으며, 식염수 주사군에서는 감소하는 경향은 있었지만 유의성은 없었다. 외양상으로 식염수 주사군의 정소에는 약간의 위축이 나타났으며, 백막이 정상적으로 보였다. 그러나 조직학적으로 정소 대부분에서 괴사가 발견되었으며, 헤마톡실린-에오신으로 거의 염색되지 않았다. 동일한 절편에서, 주사 부위의 정반대 부분은 비정상적인 세포층들이 염색되었다. 대부분의 세정관에서 미성숙한 생식세포들이 기질 막에서 분리되고, 내강 쪽으로 세포들이 떨어져 나간 것이 확인되었다. 본 연구는 정소내 고장성 식염수 직접 주사가 부속 생식기관에 정소절제와 유사한 테스토스테론 제거 효과를 유발할 수 있음을 확인하였다. 비록 심층적인 연구들이 더 요구되지만, 본 연구는 정소 내 고장성 식염수 직접 주사법이 비용이 덜 들고 덜 침습적이면서 정소절제술이나 화학적 거세와 거의 동일한 효용성을 가졌음을 시사한다.

• Archives of Plastic Surgery
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• 제33권6호
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• pp.742-747
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• 2006

Purpose: The purpose of this study was to evaluate the role of mast cell and histamine as typical product of mast cell in ischemia-reperfusion injury of muscle flap using H2 receptor blocker and mast cell stabilizer. Methods: Thirty-five Sprague-Dawley rats weighing 250-300 gm were divided into four groups; Group I: Control group without ischemia, Group II: Normal saline injection group with ischemia, Group III: Cimetidine injection group with ischemia, Group IV: Sodium cromoglycate injection group with ischemia. Well established single pedicled transverse rectus abdominis musculocutaneous(TRAM) flap was designed in all rats and were rendered ischemia by clamping the artery for 150 minutes. All injections were applied intramuscular around gluteal area 30 minutes before reperfusion. The flap survival was evaluated at 7 days after operation. Neutrophil counts and mast cell counts were evaluated 24 hours after reperfusion. Results: The difference of skin flap survival between control group and cimetidine injection group was not significant. In the normal saline injection group flap survival was markedly decreased compared to that of control group. The muscle flap survival was similar to the results of skin flap survival. The neutrophil counts were significantly decreased in control group and sodium cromoglycate injection group than normal saline injection group. The mast cell counts were significantly decreased in cimetidine injection group and control group than both normal saline injection and sodium cromoglycate injection groups. The protective effect of sodium cromoglycate was not seen in the skin flap, but the muscle flaps showed protective effects of sodium cromoglycate compared to normal saline injection group. Conclusions: It is suggests that commonly used antihistamine(H2 receptor blocker) has protective effect against ischemia-reperfusion injury to skin and muscle flaps by reducing neutrophil and mast cell. The mast cell stabilizer was not effective for skin flap but, possibly, for muscle flap.

• 한국콘텐츠학회논문지
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• 제19권11호
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• pp.445-450
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• 2019

본 연구는 자기공명영상 동적검사 시 자동주입기의 사용에 따라 조영제 역류로 인한 생리식염수의 오염 현상을 실험적으로 증명함으로써, 오염된 실린지 재사용 문제의 심각성을 인식시키고자 하였다. 연구방법은 조영제 주입 전 생리식염수와 주입 후 생리식염수를 각각 채취한 다음, T1 강조영상을 획득하여 신호강도를 측정하여 비교하였다. 생리식염수의 주입 후 신호강도가 주입 전에 비해 523.43% 통계적으로 높게 나타났다(p<0.05). 이는 조영제 주입에 따른 압력에 의해 조영제가 관을 통해 역류하여 압력이 낮은 반대쪽 생리식염수를 오염시킨 것으로 분석되었다. 결론적으로 조영제 주입 시 사용하는 실린지는 조영제 역류로 인해 교차오염이 발생하므로 정량적 분석을 위한 검사 시 동일한 환자의 검사라도 검사순서를 변경하거나 실린지를 교체하여 오류를 방지해야 한다.

• The Korean Journal of Physiology
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• 제11권1호
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• pp.21-26
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• 1977

Many cases have been reported that a post spinal headache can be relieved immediately by an epidural injection of saline; and autologous blood also has recently been used successfully instead of saline. The changes of the cerebrospinal fluid pressure in 40 cases were observed in the present study in support of the concept; that a continuous leakage in association with hypovolemia and hypotension of the cerebrospinal fluid is the primary cause of a post spinal headache. Subarachnoid pressure increased immediately with saline injection into the lumber epidural space. A spinal needle was inserted into the subarachnoid space at the level of $L2{\sim}3$ and opening pressure of the cerebrospinal fluid was read. An epidural Tuohy needle was insertad at the$L3{\sim}4$ and 25m1 of saline was injected into the epidural space and the cerebrospinal fluid pressure was read in the sitting position. $\underline{Sitting\;Position:}$ Mean pressure after injection $555{\pm}(110.9)mm\;H_2O$, Pressure rise rise (%) 51.3%, Mean opening pressure $366{\pm}(52.2)mm\;H_{2}O$, $\underline{Lateral\;position:}$ Mean pressure after injection $308{\pm}(70.8)mm\;H_{2}O$, Pressure(%) 86.7%, Mean opening pressure $165{\pm}(42.6)mm\;H_{2}O$. These pressure changes responded almost simultaneously as saline was injected. This pressure rise of 51.3% in the sitting position and 86.7% in the lateral position are clinically very significant. Therefore, it is most possible that the immediate relieve of post, spinal headache by injection of fluid into the epidural space is simultaneous with the increase of the cerebrospinal fluid pressure.

• Toxicological Research
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• 제12권1호
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• pp.101-111
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• 1996

The local irritation studies of DA-3285, recombinant human erytropoietin(rHu-EPO), were carried out in rabbits after the following treatment; single application into the conjunctival sac of the eye, single subcutaneous injection, 7-day repeated subcutaneous injection and 8-day repeated infusion into the ear vein. Also, the local irritancy of DA-3285 leaked around vein was studied in mice by single perivascular injection. The results obtained were as follows. In the result of ocular irritation test, DA-3285 could be considered as a non-irritating material. In single and 7-day repeated subcutaneous irritation test, the irritancy of DA-3285 was not so much different from that of saline. The vascular irritancy of DA3285 by 8-day repeated infusion was negligible and similar to that of saline. And the irritancy of DA3285 by perivascular injection was comparable to that of saline. These results indicate that DA-3285 has no irritating activity when injected through subcutaneous or intravenous route for clinical practice as 3.5% solution.

• 대한정형외과 초음파학회지
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• 제2권2호
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• pp.62-67
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• 2009

목적: 회전근 개 파열이 있는 환자들에서 3차원 초음파 검사를 시행할 때 생리식염수 20 ml를 견관절강 내에 주사 하기 전과 후의 파열 길이 측정과 수술시 측정과의 차이를 비교 분석하여 초음파 검사시 생리식염수의 관절내 주사가 파열의 길이 측정에 더 유용한 방법인지 확인하고자 하였다. 대상 및 방법: 2007년 8월부터 2008년 9월까지 회전근 개 파열을 진단받고 수술 받은 환자 14명에 대하여 수술 전 3차원 초음파를 이용하여 측정한 회전근 개 파열의 크기와 실제 수술 중에 측정한 파열의 크기를 비교 분석하였다. 3차원 초음파를 이용한 회전근 개 파열의 측정은 관절강 내에 20 ml의 생리식염수를 주사 하기 전, 후로 파열된 회전근 개의 너비와 내측으로의 이동 정도를 측정하였으며, 관절경 수술시에는 Kirschner 강선을 척수 바늘에 통과시키는 방법으로 파열의 길이를 측정하였고 개방적 수술시에는 자를 가지고 직접 측정하였다. 결과: 3차원 초음파 검사시 생리식염수를 주사하기 전 측정한 크기와 실제 파열된 크기를 비교하였을 때 파열의 너비의 차이는 실제 크기보다 평균 8 mm 작게 측정되었고 내측으로의 이동 정도는 평균 1.9 mm 작게 측정되었다. 생리식염수 주사 후에 측정한 결과는 초음파 검사에서 실제 크기보다 평균 4.1 mm 작게 측정되고, 내측으로의 이동의 정도는 평균 1.6 mm 크게 측정되었다. 결론: 3차원 초음파를 이용한 회전근 개 파열의 진단에서 보다 정확한 크기를 측정하기 위하여 관절강 내에 생리 식염수를 주사한 후 검사하면 보다 정확하고 실제 크기와 유사한 측정이 가능할 것으로 사료된다.

• 대한핵의학회지
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• 제5권2호
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• pp.57-64
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• 1971

The organ distribution study and the whole body scan were done in the albino rats at intervals of 5, 30, 60, 90 and 120 minutes after the intravenous injection of $^{99m}TC$-pertechnetate absorbed in Sephadex beads of $20{\sim}80$ micra in diameter. Effect of additional injection of physiological saline and saline absorbed in Sephadex beads of $20{\sim}80$ micra in diameter on the scan and organ distribution were also studied. The results were as follows. 1. Five minutes after the injection of $^{99m}TC$-pertechnetate absorbed in Sephadex beads of $20{\sim}80$ micra in diameter, Sephadex was well trapped in the lungs, with which the excellent lung scan was obtained. Two hours after the injection, kidneys were well visualized instead of lungs, which suggested that kidney acts as the excretory organ. Five minutes prior to scan, additional injection of physiological saline absorbed in Sephadex above mentioned was done. The bladder was also well visualized together with the kidneys. 2. In the distribution studies, most of radioactivity was detected in the lungs at 5 minutes and was gradually transferred chiefly to the kidneys and bladder and partly to the liver. 3. Additional injection of physiological saline resulted in a rapid transfer of $^{99m}TC$ trapped in the lung to both the kidneys and liver. 4. Additional injection of physiological saline absorbed in Sephadex beads of $20{\sim}80$ micra in diameter resulted in a rapid transfer of $^{99m}TC$ trapped in the lung to only the urinary system. 5. Results of these studies suggested that; a) Other nutrients and therapeutic compounds may be carried into the lungs along with Sephadex beads and then released in high concentration, which would exert greater therapeutic effect locally than that of the usual administration. b) Some radionuclides absorbed in Sephadex could be used as the lung scan agents, the flushing out of which by Sephadex-saline also give satisfactory renal and bladder scans. c) Other potent therapeutic radionuclides could be retained for some time by this method, which can be in the lungs easily flushed out within 2 hours.

• 대한약침학회지
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• 제4권1호
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• pp.49-53
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• 2001

This experiment has investigated the influence of Yamen (Du. 15) point injection on learning and memory dysfunction caused by cerebral ischemia and reprofusion in bilateral cervical general artery combined with bleeding on mouse tail to mimic vascular dementia in human beings. By dividing 40 mice into 4 groups (group1false operation group, group2model group, group3point injection with Cerebrolysin group4point injection with saline.) According to random dividing principles, we observed the influence of Yamen(Du. 15) point injection on the time of swimming the whole course used by model mice which had received treatment for different days in different groups, and the influence of those mice on wrong times they entered blind end. The result showed that point injection with Cerebrolysin and saline could improve learning and memory dysfunction of the mice caused by cerebral ischemia.