• 한국수산과학회지
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• 제34권6호
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• pp.697-704
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• 2001

단순 구성된 동물 먹이생물 급이 계열 (로티퍼-알테미아)을 이용하는 해산어의 종묘 생산 과정에서는 알테미아 수급 불균형 및 가격 파동 발생으로 알테미아를 대체할 수 있는 새로운 먹이생물의 개발을 요구하고 있다. 이 연구에서는 알테미아 대체 먹이생물로서 담수산 물벼룩 M. macrocopa과 기수산 물벼룩 D. celebensis의 이용 가능성을 검토하기 위한 일환으로 해수에서의 반응 결과 (생존과 증식)를 알아보았다. 담수산 물벼룩 M. macrocopa과 기수산 물벼룩 D. celebensis을 이용하여 배양 환경으로서 다양한 염분 조건으로의 점프 이동 후 두 종 물벼룩의 생존과 증식 반응을 관찰하기 위하여 16일간의 실험 기간 중 매일의 증식 상태와 단시간 생존율을 관찰하였다. 이 연구 결과, 기수산 물벼룩 D. celebensis는 0 ppt의 염분 배양 환경을 제외한 전 실험구에서 비교적 양호한 생존과 증식 반응이 관찰된 반면, 담수산 물벼룩 M. macrocopa은 염분 농도 4 ppt 이상의 점프 이동 환경하에서는 섭이 행동과 유영 행동이 급속히 둔화되면서 증식은 물론 생존조차도 불가능한 것을 알 수 있었다. 특히, 담수산 물벼룩 M. macrocopa의 단시간 생존율을 관찰한 결과에서는 3 ppt 이상의 염분 농도로 점프 이동한 경우에는 점프이동 후 5분 이내에 일부 사망 개체가 발생하는 것이 관찰되었다. 이 연구의 결과, 기수산 물벼룩 D. celebensis은 담수산 물벼룩 M. macrocopa과는 달리 현재 많은 연구가 수행되고 있는 코페포 다와 함께 해산어의 종묘 생산 과정에서 알테미아의 대체 먹이생물로 연구를 진행할 가치가 있는 것으로 판단되었다.

• 한국연초학회지
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• 제30권1호
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• pp.14-24
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• 2008

The objective of this study was to determine the effect of smoking conditions on the in vitro toxicological activity of mainstream smoke. The 2R4F reference cigarette was machine-smoked by International Organization for Standardization(ISO) and Canadian Intense(CI) conditions. Smoke was analysed for chemical composition and in vitro toxicity. The cytotoxic potencies of both the total particulate matter(TPM), which were collected in Cambridge filter pad, and gas/vapor phase(GVP), which was bubbled through in phosphate-buffer saline in a gas-washing bottle, were assessed neutral red up take assay with chinese hamster ovary(CHO) cells. The assessment for genotoxicity of TPMs generated under ISO and CI conditions was determined using Salmonella mutagenicity assay and in vitro micronucleus assay. When calculated on an equal TPM basis, in vitro toxicity of TPM obtained under CI condition was decreased compared to TPM generated under ISO condition. The results of chemical composition analyses revealed that the lower toxicological activity under CI condition than that of ISO condition could be explained by the decreased in the contents of phenols, N-nitrosoamines and aromatic amines of TPM on an equal TPM basis.

• 한국초지조사료학회지
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• 제18권3호
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• pp.171-178
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• 1998

우리나라 중서부 천결지에서 많이 자생하는 바다 새를 사료작물화 할 수 있는지를 구명하기 위하여 채종후 저장조건, 광, 옹도, 당수깊이, 염농도 및 복토깊이가 바다새의 종자 발아에 미치는 영향을 조사하였던 바, 그 결과를 요약하면 다음과 같다. 1. 바다새 종자의 발아특성은 광의 유무 간에는 유의성이 없었으며, 광의 유무 보다는 채종후 저장 조건이 유의성이 컸고, 광과 저장조건 상호간에도 유의성이 없었다. 2. 바다새의 발아율, 평균발아일수, 발아세 및 발아계수에 대한 발아온도 효과는 환온조건 보다는 변온조건에서 양호하였고, 환온조건에서는 $35^{\circ}C$에서 환온조건에서는 $35/25^{\circ}C$에서 가장 우수하게 나타났다. 3. 담수 깊이별 바다새의 발아율, 평균발아일수, 발아세 및 발아계수는 처리 수준간 통계적 유의성이 커서 담수 깊이가 Ocm인 구에서 제일 좋았으며, 담수 깊이가 0.5cm에서 1cm로 담수 깊이가 깊어 질수록 발아율이 현저히 떨어졌고 3cm 이상 담수서는 전혀 발아가 되지 않았다. 4. 고농도형 바다새의 발아율, 평균발아일수, 발아세 및 발아계수는 처리 수준간 유의성이 커서 고농도 0.2%일 때가 발아가 제일 좋았고 고농도가 높아질수록 발아율이 떨어졌으나 고농도 1%에서도 30% 이상의 발아율을 보여 내염성이 높음을 알 수 있었다. 5. 바다새 종자의 복사 수준별 발아율, 평균발아일수, 발아세 및 발아계수는 처리 수준간 유의성이 커서 복사 깊이가 0.2cm일 때가 제일 좋았고 복사 깊이가 깊어질수록 발아특성이 떨어져 복토 깊이가 1cm 이상에서는 거의 발아가 되지 않았다.5회 예취구가 37.06%로 유의하게 낮았고 3회와 4glh 예취구에서는 각각 40.27과 40.53%를 나타내어 유의성이 인정되지 않았다. 6. 건물 생산효율은 3회와 5회 예취구의 30kg N/ha/cut 수준에서 18.9kg와 15.7kg을 나타내어 가장 높았고, 4회 예취구에서는 90kg N/ha/cut 수준에서 16.3kg으로 가장 높았다. 전 질소량은 예취번초별로 3회 예취구에서는 90kg N/ha/cut수준에서 0.52kg, 4회와 5회 예취구에서는 30kg N/ha/cut수준에에서 각각 0.51kg과 0.43kg의 높은 전 질소량을 나타내었다. 7. 경제적 액상구비 시용수준의 범위는 3회 예취구에서 107.2~151.0kg N/ha/cut, 4회 예취구에서 359.1~375.7kg N/ha그리고 5회 예취구에서 160.3~236.9kg N/ha이었으며, 이 때 얻어지는 경제적 한계수량은 각각 9.6~10.0ton/ha, 12.4~12.6 ton/ha 및 9.0~9.7 ton/ha의 범위를 나타내었다. 8. 액상구비의 시용수준에 따른 액상구비의 한계시용수준은 3회 예취구에서 420.0kg/ha, 4회 예취구에서 440.6kg/ha, 그리고 5회 예취구에서 666.3kg/ha로 추정되었다.례, 오버레이 용접기술 및 용접재료의 개발현황 둥을 중심으로 살펴봄으로서 아직 국내에서는 널리 알려지지 않은 본 기 술의 활용을 넓이고자 한다. within minimum time from beginning of the shutdown.및 12.36%, $101{\sim}200$일의 경우 12.78% 및 12.44%, 201일 이상의 경우 13.

• Journal of Korean Neurosurgical Society
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• 제45권5호
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• pp.318-321
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• 2009

Tension pneumocephalus may follow a cerebrospinal fluid(CSF) leak communicating with extensive extradural air. However, it rarely occurs after diagnostic lumbar puncture, and its treatment and pathophysiology are uncertain. Tension pneumocephalus can develop even after diagnostic lumbar puncture in a special condition. This extremely rare condition and underlying pathophysiology will be presented and discussed. The authors report the case of a 44-year-old man with a basal skull fracture accompanied by pneumothorax necessitating chest tube suction drainage, who underwent an uneventful lumbar tapping that was complicated by postprocedural tension pneumocephalus resulting in an altered mental status. The patient was managed by burr hole trephination and saline infusion following chest tube disengagement. He recovered well with no neurologic deficits after the operation, and a follow-up computed tomography (CT) scan demonstrated that the pneumocephalus had completely resolved. Tension pneumocephalus is a rare but serious complication of lumbar puncture in patients with basal skull fractures accompanied by pneumothorax, which requires continuous chest tube drainage. Thus, when there is a need for lumbar tapping in these patients, it should be performed after the negative pressure is disengaged.

• 한국작물학회지
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• 제44권1호
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• pp.4-10
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• 1999

This study was conducted to determine the effects of NaCl stress on nitrogen, ${NH_4}^+$, and ${NO_3}^-$ content of 4 barley cultivar seedlings that were cultured for 10 and 30 days with different NaCl levels (0, 50, 100, and 150 mM) contain-ing 1/4 Hoagland solutions. The sodium ion content in the shoot of barley seedlings sharply increased with an increase of NaCl concentration. After 30 days of NaCl treatment, the sodium content of the shoot at 150 mM NaCl was 27 times higher than in non-saline conditions. The sodium content in the root linearly increased with increasing NaCl concentration. Nitrogen content in the shoot linearly increased with increasing NaCl concentration, but nitrogen content in the root declined above the point where the $Na^+$ content was 3.0 mM/g $Na^+$ in the barley seedling. ${NO_3}^-$ content also decreased with increasing NaCl concentration. ${NH_4}^+$ content in the shoot decreased with NaCl condition, but its content in the root increased with NaCl condition. A positive correlation between ${NO_3}^-$ and ${NH_4}^+$ content was found in the shoot, but their relationship was negative in the root.

• 한국식품영양과학회지
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• 제29권2호
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• pp.307-311
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• 2000

The purpose of this study was to identify excellent immune-enhancing substance from Korean wheats(Eunpa, Gueru, Alchan, Topdong, Suwon 267, Gobun) compared with imported ones(Australian standard white, ASW; Dark northern spring, DNS). Phagocytic activities of PBS (phosphate buffered saline, pH 7.4) and EA(ethanol-acetic acid) extracts from the wheats were determined using mouse macrophage J774 cell line. In order to set the optimal experimental condition up, the cultured cells were tested in varying experimental conditions. About two to five times higher phagocytic activity was shown in EA extract of Korean wheats compared to that of imported wheats. PBS extracts of wheats did not show increased phagocytic activity compared to control that did not add any extract. The EA extract of Gobun wheat showed the highest phagocytic activity. From the experiment we found that the optimal experimental condition was shown in two hours of reaction time and 0.05mg amout of EA extract added to J774 cells.

• 한국환경보건학회지
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• 제41권6호
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• pp.405-411
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• 2015

Objectives: The purpose of this study is to evaluate the optimum conditions for oral mucosal irritation testing using the buccal pouch of hamsters. Methods: Test materials were applied to the buccal pouch of seven-week old male Syrian hamsters (SLC, Japan) four times at one-hour intervals and macroscopic changes were examined at 24 hours after final treatment. After sacrifice, the buccal pouches were removed and prepared for histopathological evaluation. In order to set the exposure time, we performed exposure tests of 5, 12, 18 and 23 minutes using sodium lauryl sulfate (SLS) 1% and set the treatment volume from the test results at 2, 3, or 4 ml treatment using SLS 1%, Triton X-100 1% and ethanol. After setting the experimental conditions, seven groups of materials [sodium lauryl sulfate (SLS) (1%), Triton X-100 (1%), hydrogen peroxide (3%), ethanol (100%), chlorhexidine (0.2%, 2%), phosphate buffer saline (PBS)] were assessed. Results: Experimental conditions of material exposure time were fixed as 18 minutes from the exposure tests of 5, 12, 18 or 23 min using sodium lauryl sulfate (SLS) 1%. Treated volume was set as 4 ml per each pouch from the test results of 2, 3, or 4 ml treatments using SLS 1%, Triton X-100 1% and ethanol. The results in terms of irritation degree were in the order of sodium lauryl sulfate (SLS) (1%) > Triton X-100 (1%) ${\fallingdotseq}$ hydrogen peroxide (3%) > ethanol (100%) ${\fallingdotseq}$ chlorhexidine (0.2%, 2%) > phosphate buffer saline (PBS). Conclusion: From this study, suitable conditions for hamster mucosal irritation testing were suggested and this method was verified through materials commonly used on oral mucosal membranes.

• 한국해양학회지
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• 제16권1호
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• pp.31-37
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• 1981

우리나라 남동부의 광범한 유역을 따라 흐르는 낙동강은 국내에서 가장 긴 하천의 하나로서 영남지역을 굽이쳐 흐르다가 부산 앞바다를 통하여 대한해협에 유입한다. 이 강의 하구는 유역주민의 생활 및 산업용수원으로서 중요할 뿐만 아니라 최근에는 산업입지 및 자연환경 보전문제로서 논의의 대상이 되고 있다. 낙동강은 하구로부터 약 40km상류까지 상향류를 보이며, 약 60km상류까지 조석의 영향을 받는 감조하천으로서 (유 장 1979), 하구역에는 해수, 하천의 담수 및 양자의 혼합수가 조석의 간만등 여러 가지 요인에 따라 유출되기도 하고 유입되기도 한다.

• 한국환경보건학회지
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• 제34권6호
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• pp.452-458
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• 2008

This study was undertaken to establish the biological reaction characteristics of the marine products industry wastewater which contains high concentrations of organic matter and saline. As the S/I is varied from 0.3 to 1.2, the results were follows : the observed ultimate anaerobic biodegradability varied from 72.0 to 88.0%, the first order reaction rate varied from 0.1735 to $0.3420\;day^{-1}$ and the second order reaction rate varied from 0.0132 to $0.0295\;day^{-1}$. When S/I was 0.9, the first order reaction rate had a maximum value, but the variations of the second order reaction rate were less than 1st-order reaction rate. When the operation time exceeded 2 days the gas production rapidly increased. The source of this rapid increase was due to that the activity of the granular sludge used in this study being faster than that of conventional sludge. Under aerobic condition, the characteristics of organic matter were as follows: the marine industry wastewater used in this study contained about 81% of biodegradable matter, and it was divided into readily biodegradable COD(Ss), slowly biodegradable COD(Xs), soluble COD(Si) and inert suspended COD (Xi). The percentages of each COD were 87.3%, 23.9%, 6.4% and 12.4% respectively.

• 대한약침학회지
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• 제21권2호
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• pp.104-111
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• 2018

Objective:This study is to evaluate both the single-dose intravenous injection toxicity and the approximate lethal dose of Water-soluble Danggui Pharmacopuncture (WDP) in Sprague-Dawley (SD) rats. Methods: Toxicity experiments were conducted at Good Laboratory Practice (GLP) laboratory in Biotoxtech Co., according to the regulations of GLP. WDP injection of dose 0.125, 0.25, and 0.5 mL/animal were experimental groups and normal saline injection group was control group. WDP and normal saline were injected once to 6- week old 5 male and 5 female SD rats at the tail veins at approximately 2 mL/min. During 14 days after the injection, general symptoms were observed and weight were measured. After the observation period, hematological and blood biochemical examination, macroscopic autopsy, topical resistance test at the injection area were performed. Results: RThe WDP 0.5 mL/animal injection group in 4 cases of male rats and all cases of female rats showed hematuria 30 minutes after the administration. However, after 1 hour, no more abnormal general symptoms were observed. The WDP did not affect weight, hematological and blood biochemical examination, macroscopic autopsy, and topical resistance test at the injection area. Conclusion: WDP single dose intravenous injection results showed that WDP have no toxic effects and a lethal dose of WDP should be over 0.5 mL/animal in male and female rats under the study condition. So WDP may be safe.