Journal of the Korean Society of Tobacco Science (한국연초학회지)

The Korean Society of Tobacco Science (한국연초학회)
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Effect of smoking conditions on the biological activity of cigarette mainstream smoke

담배 주류연의 생물학적 활성에 대한 흡연조건의 영향

  • Published : 2008.06.25
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Abstract

The objective of this study was to determine the effect of smoking conditions on the in vitro toxicological activity of mainstream smoke. The 2R4F reference cigarette was machine-smoked by International Organization for Standardization(ISO) and Canadian Intense(CI) conditions. Smoke was analysed for chemical composition and in vitro toxicity. The cytotoxic potencies of both the total particulate matter(TPM), which were collected in Cambridge filter pad, and gas/vapor phase(GVP), which was bubbled through in phosphate-buffer saline in a gas-washing bottle, were assessed neutral red up take assay with chinese hamster ovary(CHO) cells. The assessment for genotoxicity of TPMs generated under ISO and CI conditions was determined using Salmonella mutagenicity assay and in vitro micronucleus assay. When calculated on an equal TPM basis, in vitro toxicity of TPM obtained under CI condition was decreased compared to TPM generated under ISO condition. The results of chemical composition analyses revealed that the lower toxicological activity under CI condition than that of ISO condition could be explained by the decreased in the contents of phenols, N-nitrosoamines and aromatic amines of TPM on an equal TPM basis.

Keywords

References

  1. Ames, B. N., McCann, J. and Yamasaki, E. (1975) Methods for detecting carcinogens and mutagens with the Salmonella/ mammalian microsome mutagenicity test. Mutat. Res. 31: 347-364 https://doi.org/10.1016/0165-1161(75)90046-1
  2. Ashby, J., Brady, A., Elcombe, R., Elliott, B. M., Ishmael, J., Odum, J., Tugwood, J. D., Kettle, S. and Purchase, I. F. H. (1994) Mechanistically-base human hazard assessment of peroxisome proliferator-induced hepatocarcinogenesis, Human & Exp. Toxicol. 13: S1-S117 https://doi.org/10.1177/0960327194013001011
  3. Balls, M. and Clothier, R. H. (1992) Cytotoxicity assays for intrinsic toxicity and irritancy. In: Watson, R.R. (Ed), In Vitro methods of Toxicology. CRC Press, Boca Raton, pp. 37-52
  4. Belinsky, S. A., Walker, V. E., Maronpot, R. R., Swenberg, J. A. and Anderson M. W. (1987) Molecular dosimetry of DNA adduct formation and cell toxicity in nasal mucosa following exposure to a specific carcinogen: Relationship to induction of neoplasia. Cancer Res. 47: 6058-6065
  5. Bombick, D. W., Bombick, B. R., Swauger, J. and Doolittle, D. J. (2001) The use of in vito short term tests to evaluate progress toword reducing the toxicity of cigarette smoke. CORESTA Meet. Smoke-Techno Group, Xian: Paper No. ST4
  6. Bombick, D. W., Bombick, B. R., Ayres, P. H., Putnam, K., Avalos, J., Borgerding, M. F. and Doolittle, D. J. (1997) Evaluation of the genotoxic and cytotoxic potential of mainstream whole smoke and smokecondensate from a cigarette containing a novel carbon filter. Fund. Appl. Toxicol. 39: 11-17 https://doi.org/10.1006/faat.1997.2334
  7. Borenfreund, E. and Puerner, J. A. (1985) Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol. Lett. 24: 119-124 https://doi.org/10.1016/0378-4274(85)90046-3
  8. Buckton, K. E. and Evans, H. J. (1973) Method for the analysis of human chromosome abberration. World Health Oranization, Geneva, Switzerland pp.18
  9. Chen, Q., Jones, T. W., Brown, P. C. and Steven, J. L. (1990) The mechanism of cysteine conjugate cytotixicity in renal epithelial cells: Covalent binding leads to thiol depletion and lipid peroxidation. J. Biol. Chem. 265: 21603-21611
  10. Cooper, R. L., Lindsey, A. J. and Wallwe, R. E. (1954) The presence of 3,4-benzopyrene in cigarette smoke. Chem. Ind. 46: 14-18 https://doi.org/10.1021/ie50537a901
  11. Count, M. E., Morton, M. J., Laffoon, S. W. Cox, R. H. and Lipowicz, P. J. (2005) Smoke composition and predicting relationships for international commercial cigarettes smoked with three achine-smoking conditions. Regulatory Toxicol. Pharm. 41: 185-227 https://doi.org/10.1016/j.yrtph.2004.12.002
  12. Curvall, M., Enzell, C. R. and Pettersson, B. (1984) An evaluation of the utility of four in vitro short term tests for predicting the cytotoxicity of individual compounds derived from tobacco smoke. Cell Biol. Toxicol. 1: 173-193 https://doi.org/10.1007/BF00125573
  13. Doolittle, D. J., Lee, C. K., Ivett, J. L., Mirsalis, J. C., Riccio, E., Rudd, C. J., Burger, G. T. and Hayes A. W. (1990) Comparative studies on the genotixic activity of mainstream smoke condendsate from cigarettes which burn or only heat tobacco. Enviromental and Molecular Mutagenesis 15: 93-105 https://doi.org/10.1002/em.2850150206
  14. Fenech, M. (2000) The in vitro micronucleus technique. Mutat. Res. 455: 81-95 https://doi.org/10.1016/S0027-5107(00)00065-8
  15. Fenech, M. and Morley, A. A. (1985) Measurement of micronucleic in lymphocytes. Mutat. Res. 147: 29-36 https://doi.org/10.1016/0165-1161(85)90015-9
  16. Health Canada (1999a) Determination of selected carbonyls in mainstream tobacco smoke. Tobacco Control Programen Health Canada- Official method T-104
  17. Health Canada (1999b). Determination of tar, Nicotine and Carbon Monoxide in Mainstream Tobacco Smoke. Official method T-115
  18. Health Canada (1999c). Determination of 1,3-butadiene, isoprene, acrylonitrile, bensene, and toluene in mainstream tobacco smoke. Official method T-116
  19. Health Canada (1999d). Determination of pyridine, quinoline and styrene in mainstream tobacco smoke. Official method T-112
  20. Health Canada (2004). Neutral Red Uptake Assay for Mainstream Tobacco Smoke. Official method T-502
  21. Hoffmann, D., Hoffmann, I. and EI-Bayoumy, K. (2001) The less harmful cigarette : a controversial issue. A tribute to Ernst L. Wynder. Chem. Res. Toxicol. 14: 767-790 https://doi.org/10.1021/tx000260u
  22. ISO Standard 3402, fourth ed., (1999) International Organization for Standardization. Tobacco and tobacco productsatmosphere for conditioning and testing
  23. ISO Standard 4387, third ed., (2000a) International Organization for Standardization. Cigarettes-determination of total and nicotine-free dry particulate matter using a routine analytical smoking machine
  24. ISO Standard 3308, fourth ed., (2000b) International Organization for Standardization. Routine analytical cigarette smoking machine-definitions and standard conditions
  25. ISO Standard ISO 10315, second ed. and Corrgendum I, (2000c) International Organization for Standardization. Cigarettes determination of nicotine in smoke condensates-gas chromatographic method
  26. Kirsch-Volders, M., Elhajouji, A., Cundari, E. and VanHummelen, P. (1997) The in vitro micronucleus test : a multipoint assay to detect simutaneously mitotic delay, apoptosis, chromosome breakage, chromosome loss and non-disjunction. Mutat. Res. 392: 19-30 https://doi.org/10.1016/S0165-1218(97)00042-6
  27. Manabe, S. and Wada, O. (1990) Carcinogenic tryptophan pyrolysis products in cigarette smoke condensate and cigarette smokepolluted indoor air. Environ. Pollute. 64: 121-132 https://doi.org/10.1016/0269-7491(90)90109-P
  28. Maron, D. M. and Ames, B. N. (1983) Revised methods for the Salmonella mutagenicity test. Mutat. Res. 113: 173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  29. Massachusetts General Laws, (1997) Chapter 94, Sect. 307B, 105 Code of Massachusetts Regulations 660.000 et seq
  30. Miller, S., Potter-Locher, F., Seelbach, A., Stopper, H., Utesch, D. and Madle, S. (1998) Evaluation of the in vitro micronucleus test as an alternative to the in vitro chromosomal aberration assay: position of the GUM working group on the in vitro micronucleus test. Mutat, Res. 410: 81-116 https://doi.org/10.1016/S1383-5742(97)00030-6
  31. Mortelmans, K. and Errol Zeiger, E. (2000) The Ames Salmonella/microsome mutagenicity assay. Mutat. Res., 455: 29-60 https://doi.org/10.1016/S0027-5107(00)00064-6
  32. Muller, L. and Peter Kasper, P. (2000): Human biological relevance and the use of threshold-arguments in regulatory genotoxicity assessment: experience with pharmaceuticals. Mutat. Res. 464: 19-34 https://doi.org/10.1016/S1383-5718(99)00163-1
  33. Peeler, C.L., (1996) Cigarette testing and the Federal Trade Commission: A historical overview. In: The FTC Cigarette Test Method for Determining Tar, Nicotine, and Carbon Monoxide Yields of U.S. Cigarettes; Report of the NCI Expert Committee. Smoking and Tobacco Control Monograph No. 7. Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Bethesda, MD, NIH Pub. No. 96-4028 (Chapter 1)
  34. Phillips, D. H. (2002) Smoking-related DNA and protein adducts in human tissues. Carcinogenesis 23: 1979-2004 https://doi.org/10.1093/carcin/23.12.1979
  35. Rickert, W. S., Trivedi, A. H., Momin, R. A. Wright, W. G. and Lauterbach, J. (2007) Effect of Smoking Conditions and Methods of collection on the Mutagenicity and Cytotoxicity of Cigarette Mainstream Smoke. Toxicol. Sci. 96: 285-293 https://doi.org/10.1093/toxsci/kfl195
  36. Sanders, C., Swedlund, T. D., Stephens, T. J. and Silber, P. M. (1991) Evaluation of six in vitro tixicity studies: Comparison with in vivo ocular and dermal irritation potential of prototype cosmetic formulations. Toxicologist 11: 282-289
  37. Shirhatti, V. and Krishna, G. (1990) A simple and sensitive method for monitoring drug induced cell injury in cultured cells. Analytical Biochemistry 147: 410-418 https://doi.org/10.1016/0003-2697(85)90290-8
  38. Tewes, F. J., Meisgen, T. J., Veltel, D. J., Roemer, E. and Patskan, G. (2003) Toxicological evaluation of electrically heated cigarette. Part 3: genotoxicity and cytotoxicity of mainstream smoke. J. Appl. Toxicol. 23: 341-348 https://doi.org/10.1002/jat.925
  39. Yamasaki, H., Ashby, J., Bignami, M., Jongen, W., Linnainmaa, K., Newbold, R. F., Nguyen-Ba, G., Parooi, S., Rivedal, E., Schiffmann, D., Simons, J. W. and Vasseur, P. (1996) Non-genotoxic carcinogens: development of detection methods based on mechanisms: a european project. Mutat. Res. 353: 47-63 https://doi.org/10.1016/0027-5107(95)00241-3