• 미생물학회지
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• 제24권1호
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• pp.7-11
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• 1986

Pseudomonas의 분해계 플라스미드와 이들에 유용한 벡터를 개발하기 위해서 본 연구실에서 분리, 보존하고 있는 Pseudomonas 중에서 P.putita KU 190으로부터 하나의 플라스미드를 분리하여 그 특성을 해석코저 하였다. size marker로 RP4와 pSy343를 사용하여 플라스미드의 크기를 결정한 바 41Kb로 나타났으며, 이 플라스미드를 pKU 41라 명명하였다. 제한효소 Bglll, BamHl, Eco Rl, HindIII, Sall등으로 이 플라스미드를 소화하여 이들의 제한효소 pattern을 조사한 결과, Bg III가 1. BamHl 3, Hin dIll는 3, EcoRI은 6 그리고 SalI은 13개 이상의 절단부위를 가지고 있었다. 이 제한효소의 절단부위, 토막들의 크기로부터 BamHI, HindIII에 대한 지도를 작성하였다. 플라스미드의 생울학적 기능을 조사하기 위하여 탄화수소 자화능에 대한 큐어링 실험을 하였으나 이로부터 뚜렷한 관련성 여부를 관찰할 수 없었다.

• 한국발생생물학회지:발생과생식
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• 제23권1호
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• pp.43-54
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• 2019

We examined the effects of endoplasmic reticulum (ER) stress inhibitor treatment during the micromanipulation of porcine somatic cell nuclear transfer (SCNT) on the in vitro development of SCNT embryos. ER stress inhibitors such as salubrinal (200 nM) and tauroursodeoxycholic acid (TUDCA; $100{\mu}M$) were added to the micromanipulation medium and holding medium. The expression of X-box binding protein 1 (Xbp1), ER-stress-associated genes, and apoptotic genes in SCNT embryos was confirmed at the one-cell and blastocyst stages. Levels of Xbp1 splicing and expression of ER-stress-associated genes in SCNT embryos at the one-cell stage decreased significantly with TUDCA treatment (p<0.05). The expression of ER-stress-associated genes also decreased slightly with the addition of both salubrinal and TUDCA (Sal+TUD). The expression levels of caspase-3 and Bcl2-associated X protein (Bax) mRNA were also significantly lower in the TUDCA and Sal+TUD treatments (p<0.05). At the blastocyst stage, there were no differences in levels of Xbp1 splicing, and transcription of ER-stress-associated genes and apoptosis genes between control and treatment groups. However, the blastocyst formation rate (20.2%) and mean blastocyst cell number ($63.0{\pm}7.2$) were significantly higher (p<0.05) for embryos in the TUDCA treatment compared with those for control (12.6% and $41.7{\pm}3.1$, respectively). These results indicate that the addition of ER-stress inhibitors, especially TUDCA, during micromanipulation can inhibit cellular damage and enhance in vitro development of SCNT embryos by reducing stress levels in the ER.

• 대한한의학방제학회지
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• 제30권3호
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• pp.109-121
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• 2022

Objective : This study is conducted to investigate the effect of Astragali Radix on Post Traumatic Stress Disorder(PTSD) induced by the Single Proposed Stress(SPS). Methods : The experiment was conducted with five groups; SAL groups with only saline treatment, SPS group, SPS + ARX25 group, SPS + ARX50 group, and SPS + ARX100 group. After applying SPS, saline and ARX were administered for 14 days to identify the change of body weight, sucrose intake amount, and behavioral changes through Open Field Test(OFT) and Forced Swimming Test(FST). After the behavioral experiment, plasma corticosterone levels, serotonin, norepinephrine and dopamine concentrations were measured by enzyme-linked immunoassay in medical prefrontal cortex, hippocampus, and amygdala. Brain-derived neurotrophic factor(BDNF) in the hippocampus was measured using Reverse Transcription Polymerase Chain Reaction. Results : Weight change has significantly decreased in the SPS group compared to the SAL group(p<0.05). On day 14, the sucrose intake of rats has significantly increased in the SPS + ARX100 group compared to the SPS group(p<0.05). In OFT, the number of staying in the central space has significantly increased in the SPS + ARX100 group(p<0.01). In FST, immobility has significantly decreased in SPS + ARX50 group and SPS + ARX100 group(p<0.05). The concentration of serotonine, dopamine and BDNF expression has increased significantly in SPS + ARX100 group compared to SPS group(p<0.05) Conclusions : In the SPS-induced PTSD experiment, ARX increased sucrose intake and the numbers of crossing in the central zone space in OFT, decreased immobility time in FST, and increased concentration of serotonin, dopamine, and BDNF. It can be postulated that the ARX could be effective for the treatment of PTSD.

• Tuberculosis and Respiratory Diseases
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• 제59권2호
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• pp.164-169
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• 2005

연구배경 : ICS/LABA는 천식에서 효과가 입증되었으나 COPD에서는 덜 알려져 있다. 최근 COPD 환자에서 고용량의 ICS/LABA의 사용시 급성악화를 줄인다고 보고되고 있다. 본 연구는 중등도와 고용량의 ICS/LABA사용시 용량에 차이에 따른 COPD의 급성악화를 비교하였다. 방 법 : 2001년 1월 1일부터 2004년 8월 31일 사이에 고대안암병원에 내원하여 진단된 46명의 중등증 이상의 COPD 환자를 대상으로 하였다. flu/sal $250{\mu}g/50{\mu}g$ 하루 2번 사용한 군(A군)과 flu/sal $500{\mu}g/50{\mu}g$ 하루 2번 사용한 군(B군)으로 나누어 1년 기간 동안 급성악화의 횟수 및 입원 횟수, 처음악화까지 걸린 기간을 분석하였다. 결 과 : A 군 26명중 11명에서 급성악화를 경험하였고, 평균 악화 횟수는 0.96회, 입원횟수는 0.15 회였다. B군 20명중 11명에서 급성 악화를 경험하였고 평균 악화 횟수는 1.05회, 입원횟수는 0.30 회였다. 두 군간에 통계적으로 유의한 차이가 없었고 처음악화까지 걸린 시간도 차이가 없었다. 결 론 : 중등증 이상의 COPD 환자에서 ICS 와 LABA 복합제제를 사용 할 때 고농도와 중등도 용량에서 급성 악화에 미치는 영향은 통계적으로 차이가 없었다. 향후 COPD 에서 ICS/LABA 사용시 효과적인 용량에 대한 연구가 좀 더 필요할 것으로 사료된다.

• 한국식품조리과학회:학술대회논문집
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• 한국식품조리과학회 2003년도 춘계학술대회
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• pp.89-89
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• 2003

가정배달급식의 저장기간 중 식중독 발생에 대비하여 식중독균의 중식 양상을 사전에 예측하기 위한 기본자료를 얻고자 표고고기전, 갈치조림, 더덕구이에 주요 식중독 원인균인 Sal. enteritidis, V. parahaemolyticus, S. aureus, E. coli O157:H7, L. monocytogenes를 접종하여 wrap 포장, 상압 및 진공포장 처리한 후, -18$^{\circ}C$, 4$^{\circ}C$, $25^{\circ}C$에서 5일간 포장방법 및 저장조건에 따른 변화를 살펴보야T다. 가정배달급식을 위한 음식에 주요 식중독균 접종 결과, 진공포장 처리 후 냉장 및 냉동저장 시 식중독균의 증식이 억제되는 것으로 입증되었고, 접종된 주요 식증독균의 포장방법 및 저장조건에 따른 성장 및 증식은 저장온도에 유의적인 영향을 받는 것으로 나타났으며, 특히, L monocytogenes는 저장기간에 대해서도 유의적인 영향을 받는 것으로 나타났다. (중략)

• Bulletin of the Korean Chemical Society
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• 제24권6호
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• pp.731-732
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• 2003

Mutagen X (MX), 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone is one of the most potent directing acting mutagen ever tested in SAL TA100 assay. Although MX analogues have been synthesized, tested for mutagenicity and modeled by structure-activity relationship (SAR) methods, the mechanism of interaction of these compounds with DNA to produce their remarkable mutagenic potency remains unresolved. MX exists as an equilibrium mixture of both ring and open form in water. This equilibrium is very fast for Ames test. Because the mixture is not separable by experimental methods, it is not clear which one is really responsible for the observed mutagenicity. There have been many debates that which one is really responsible for the observed mutagenicity. We used ab initio methods for the MX analogues. It seems both ring and open form could react with DNA bases as electrophiles. However, every open form has consistently lower LUMO energy than corresponding ring form. It is reasonable to assume that the major reaction will go through via open form for MX analogues. This suggest that the open form is more likely really mutagenic.

• 한국환경농학회지
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• 제17권4호
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• pp.368-370
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• 1998

In order to determine the optimal radiation dose for the sterilization of biowastes, the bioburden, frequencies and radiosensitivities of bacteria, mold and fungi in rice straw, chaff, corn stover and sawdust was observed before and after ${\gamma}-ray$ irradiation. Radiation sterilization dose of rice straw, chaff, com stover and sawdust was calculated as 17.7, 17.6, 15.6 and 20.0kGy, respectively, from the mutual compensation of screening dose and derived dose on the basis of $10^{-3}$ SAL. This method could be acceptable for the sterilization of various biowastes including food, pharmaceuticals, etc.

• 한국가구학회지
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• 제18권2호
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• pp.129-137
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• 2007

Total forest land in Bangladesh is about 2.5 million hectare which is 17% of total land area. Bangladeshi forest is mainly classified into three categories- 1) Hill forest 2) Sal forest and 3) Mangroves. This forest land area is decreasing day by day. But in past decayed due to some policies, the total forest land area is increasing slowly. By this planning both government and the local people in the community are being benefited. This paper mainly discussed about the past, present and future trend of forest condition, forest land uses, sustainable forest planning and forest management.

• 대한수학회보
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• 제45권1호
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• pp.157-167
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• 2008

A mapping f : $M{\rightarrow}N$ between Hilbert $C^*$-modules approximately preserves the inner product if $$\parallel<f(x),\;f(y)>-<x,y>\parallel\leq\varphi(x,y)$$ for an appropriate control function $\varphi(x,y)$ and all x, y $\in$ M. In this paper, we extend some results concerning the stability of the orthogonality equation to the framework of Hilbert $C^*$-modules on more general restricted domains. In particular, we investigate some asymptotic behavior and the Hyers-Ulam-Rassias stability of the orthogonality equation.

• International Journal of Industrial Entomology and Biomaterials
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• 제26권1호
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• pp.1-12
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• 2013

The commercial rearing of polyphagous Indian tasar silkworm, Antheraea mylitta Drury being practiced on naturally grown primary food plants like Terminalia arjuna, (Arjun) Terminalia tomentosa (Asan), and Shorea robusta (Sal) available in the tropical forests of central India, at times, is seriously affected by the disease- Pebrine, caused by Nosema sp., a microsporidian pathogen. The present investigation on comparative larval, silk gland weight and also cocoon parameters in Pebrine-free and Pebrine-infected ecorace of tasar silkworm Antheraea mylitta Drury (Daba TV), illustrates the tasar silkworm larvae infected with pebrine disease causing heavy losses to the economy of the silk industry.