• 제목/요약/키워드: Safety test

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Selecting Aquifer Artificial Recharge Methods Based on Characteristics of the Target Aquifer (주입대상 대수층의 특성을 고려한 인공함양 방법 선정 연구)

  • Lee, Yeoung-Dong;Shin, Dong-Min;Kim, Byeong-Jun;Kim, Gyoo-Bum
    • The Journal of Engineering Geology
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    • 제29권4호
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    • pp.483-494
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    • 2019
  • This study aimed to determine the extent of artificial aquifer recharge and to evaluate appropriate recharge techniques based on field investigations and comparative analysis of each recharge method. Characteristics of the aquifer determine the target aquifer and the recharge method for artificial groundwater recharge. Electrical conductivity surveys, drilling, permeability tests, and grain-size analysis indicate that the hydraulic conductivity of weathered soil and weathered rock is higher than that of upper unconsolidated soil. Pumping tests indicate that the groundwater level was stable at a depth of 12 m until 9 hours of pumping, but after that it dropped again, indicating anisotropic aquifer characteristics. Three types of artificial recharge method were reviewed, including recharge wells, ditches, and ponds, and a combination of two methods is proposed: a recharge well system directly injecting into weathered soil and rock sections with good permeability, and an injection ditch that can increase the recharge effect by line-type injection in the upstream area. The extent of groundwater recharge by the selected methods will be evaluated through on-site tests and if their applicability is verified, they will contribute to securing water in areas of water shortage.

Cloning of Farm Animals in Japan; The Present and the Future

  • Shioya, Yasuo
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 한국발생생물학회 2001년도 발생공학 국제심포지움 및 학술대회 발표자료집
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    • pp.37-43
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    • 2001
  • 1. About fifty thousand of cattle embryos were transferred and 16000 ET-calves were born in 1999. Eighty percents of embryos were collected from Japanese Black beef donors and transferred to dairy Holstein heifers and cows. Since 1985, we have achieved in bovine in vitro fertilization using immature oocytes collected from ovaries of slaughterhouse. Now over 8000 embryos fertilized by Japanese Black bull, as Kitaguni 7~8 or Mitsufuku, famousbulls as high marbling score of progeny tests were sold to dairy farmers and transferred to their dairy cattle every year. 2. Embryo splitting for identical twins is demonstrated an useful tool to supply a bull for semen collection and a steer for beef performance test. According to the data of Dr. Hashiyada(2001), 296 pairs of split-half embryos were transferred to recipients and 98 gave births of 112 calves (23 pairs of identical twins and 66 singletons). 3. A blastomere-nuclear-transferred cloned calf was born in 1990 by a joint research with Drs. Tsunoda, National Institute of Animal Industry (NIAI) and Ushijima, Chiba Prefectural Farm Animal Center. The fruits of this technology were applied to the production of a calf from a cell of long-term-cultured inner cell mass (1988, Itoh et al, ZEN-NOH Central Research Institute for Feed and Livestock) and a cloned calf from three-successive-cloning (1997, Tsunoda et al.). According to the survey of MAFF of Japan, over 500 calves were born until this year and a glaf of them were already brought to the market for beef. 4. After the report of "Dolly", in February 1997, the first somatic cell clone female calves were born in July 1998 as the fruits of the joint research organized by Dr. Tsunoda in Kinki University (Kato et al, 2000). The male calves were born in August and September 1998 by the collaboration with NIAI and Kagoshima Prefecture. Then 244 calves, four pigs and a kid of goat were now born in 36 institutes of Japan. 5. Somatic cell cloning in farm animal production will bring us as effective reproductive method of elite-dairy- cows, super-cows and excellent bulls. The effect of making copy farm animal is also related to the reservation of genetic resources and re-creation of a male bull from a castrated steer of excellent marbling beef. Cloning of genetically modified animals is most promising to making pig organs transplant to people and providing protein drugs in milk of pig, goat and cattle. 6. Farm animal cloning is one of the most dreamful technologies of 21th century. It is necessary to develop this technology more efficient and stable as realistic technology of the farm animal production. We are making researches related to the best condition of donor cells for high productivity of cloning, genetic analysis of cloned animals, growth and performance abilities of clone cattle and pathological and genetical analysis of high rates of abortion and stillbirth of clone calves (about 30% of periparutum mortality). 7. It is requested in the report of Ministry of Health, labor and Welfare to make clear that carbon-copy cattle(somatic cell clone cattle) are safe and heathy for a commercial market since the somatic cell cloning is a completely new technology. Fattened beef steers (well-proved normal growth) and milking cows(shown a good fertility) are now provided for the assessment of food safety.

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SPF Measurement and Cytotoxicity of Sunscreen Agents in Cosmetic (화장품에서 UV 차단제의 피부 자극성과 SPF 측정)

  • Kim, In-Young;Kang, Sam-Woo
    • Analytical Science and Technology
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    • 제11권2호
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    • pp.79-87
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    • 1998
  • Consumers have recently preferred to purchase extensive UV intercepting products, which are waterproof and free from side effects on skin. During the testing of cytotoxicity (in-vitro) in neutral red (NR) method, cell survival ratio of UV-B interceptors decreased to just above 0.08 w/v%, and it was observed that the UV-A interceptors the ratio also decreased to just above 0.06 w/v%. In addition patch-tests of inorganic UV interceptors resulted in no skin irritation even below 10.0 and 11.25. In absorption curves, UV-B was most suitable for octyl methoxycinnamate (OMC) and UV-A for butyl methoxy dibenzoylmethane (BMDM). For this reason, $Nylonpoly^{TM}$ UVA/UVB the material of OMC and BMDM coated with Nylon & polyethylene, was used as the organic UV interceptor. Zinc oxide (ZnO) and titanium dioxide ($TiO_2$) was used as inorganic UV interceptors. The appropriate mixture ratio of ZnO and $TiO_2$ was 6 to 4:6% of ZnO, 4% of $TiO_2$ and 5% of $Nylonpoly^{TM}$ UVA/UVB were all combined and added to our sunscreen cream. The SPF value of in-vitro was 38.9. In practical application, each sun protection factor (SPF) duration of oil-in-water (O/W) emulsion and water-in-silicone (W/S) emulsion containing sunscreen cream of the same content showed that W/S type of sunscreen cream was 5 times as durable as the other. Therefore, this product is fit for use in swimming, climbing or skiing. This research is to minimize skin trouble caused by UV interceptors and to make one with proper softness, skin safety and UV intercepting efficiency.

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A Study on the Efficiency Estimation of Halogen free Fire Resistance Cable (저독성 내화전선 케이블의 성능평가에 관한 연구)

  • 윤헌주;홍진웅;유동일;윤재선;곽동일
    • Fire Science and Engineering
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    • 제16권1호
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    • pp.18-23
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    • 2002
  • Efficiency estimation of toxicity free resistance cable experiments were conducts to understand toxicity free fire ersistance polyolefin insulation material and smoke density characteristic and combustion gas corrosion analysis. A main cause of fire-growth and generating toxic gas when it burns, should be dealt with great care in life safety design. Similar patterned fire incidents such as, Inchon Live-Hof Pub Restaurant as, Sea-land Children Resort have proven that serious loss of lives were caused by hazardous gas generated fire resistance cable materials. In this paper, Referenced documents were ASTM E662 standard test method for specific Ds genalated by solid materials. The furnace control system shall maintain the required irradiance level under steady-state condition with the chamber door closed of 2.5$\pm40.04〔w/$\textrm{cm}^2$〕for 20 min. According to the results of the smoke density analysis of NFR-8 and FR-PVC the highest decomposition flaming smoke density range of NFR-8 and FR-PVC were 25.2 to 37.5 and 51.1 respectively. Nonflaming smoke density range of NFR-8 and FR-PVC were 100.4 to 112.2 and 126.5 to 398.8. Also, the fire gases was occurred carbon monoxide and decomposition than in polyolefin due to incomplete combustion of PVC which has high content of carbon in chemical compound.

Effective Components of Commercial Enzyme Food Products and Their HACCP Scheme (시판 효소식품의 유용성분과 HACCP 관리 방안에 관한 연구)

  • Lee, Eun-Joo;Lee, Cherl-Ho
    • Korean Journal of Food Science and Technology
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    • 제33권4호
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    • pp.461-468
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    • 2001
  • The effectiveness and safety of Enzyme Food, a group of dietary supplements designated by Korean Food Law, were evaluated and the possibility of HACCP (Hazard Analysis Critical Control Point) application was investigated. Chemical composition, enzyme activities and the degree of bacterial contamination in 12 samples of different brands sold in Korean market were measured. The chemical composition of the selected products varied and inconsistent to those claimed in the label description. It is known that effectiveness of Enzyme Food depends on enzyme activity, but enzyme activities of ${\alpha}-amylase$ varied from $1,793\;{\mu}g/min$ g to $159\;{\mu}g/min$ g and those of ${\beta}-amylase$ ranged from $171\;{\mu}g/min$ g to $11\;{\mu}g/min$ g. The protease activities varied from $27.57\;{\mu}g/min$ g to $0.18\;{\mu}g/min$ g. In coli-form bacterial test, positive reactions were appeared in the 50 % of the samples. Numbers of bacteria ranged from $1.3\;{\times}\;10^5\;to\;1.2\;{\times}\;10^9$. Five CCPs were identified; heating, inoculation, cultivation, drying and granulation. Consideration of HACCP system indicated that the pretreatment of raw material, checking of bacterial contamination and stability of enzyme activity during fermentation process were important factors for the quality of Enzyme Foods.

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Effect of Heat, Pressure, and Acid Treatments on DNA and Protein Stability in GM Soybean (GM 콩 DNA와 단백질의 안정성에 대한 열, 압력 및 산 처리의 영향)

  • Pack, In-Soon;Jeong, Soon-Chun;Yoon, Won-Kee;Park, Sang-Kyu;Youk, Eun-Soo;Kim, Hwan-Mook
    • Korean Journal of Food Science and Technology
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    • 제36권4호
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    • pp.677-682
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    • 2004
  • Debates on safety of genetically modified (GM) crops have led to mandatory-labeling legislation of GM foods in many countries including Korea. Effects of heat, pressure, and acid treatments on degradation of DNAs or proteins in GM soybean at levels below detection limits of qualitative PCR and lateral flow strip test (LFST) methods were examined. Results showed that genomic DNAs and proteins were degraded into fragment sizes no longer possible for detection of inserted gene depending on thermal, or thermal and pressure treatment period. Detectaability of LFST for toasted meal increased in weakly treated soybean. DNA and protein detection methods were barely effective for detection of GM ingredient after $121^{\circ}C$ and 1.5 atmospheric treatment for 20 min. These results will be useful in determining GM labeling requirements of processed foods.

The Association of Heavy Metal of Blood and Serum in the Alzheimer's Diseases

  • Lee, Ji-Yeon;Kim, Jae-Hoon;Choi, Dal-Woong;Lee, Dong-Woo;Park, Jun-Hyun;Yoon, Hye-Jung;Pyo, Hee-Soo;Kwon, Ho-Jang;Park, Kyung-Su
    • Toxicological Research
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    • 제28권2호
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    • pp.93-98
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    • 2012
  • This study has attempted to establish an analysis method through validation against heavy metals in the body (Pb, Cd and Hg) using ICP-MS and Gold amalgamation and find out the relevance between heavy metal and Alzheimer's disease after analyzing the distribution of heavy metal concentration (Pb, Cd and Hg) and correlations between a control group and Alzheimer's disease group. In this study, Pb and Cd levels in the blood and serum were validation using ICP-MS. For analysis of Hg levels in the blood and serum, the gold amalgamation-based 'Direct Mercury Analyzer' has been used. According to an analysis on the heavy metal concentration (Pb, Cd and Hg concentration) in the blood, Cd concentration was high in the Alzheimer's disease group. In the serum, on the contrary, Pb and Hg were high in the Alzheimer's disease group. For analysis of correlations between heavy metal levels in the blood and serum and Alzheimer's disease, t-test has been performed. Even though correlations were observed between the blood lead levels and Alzheimer's disease, they were statistically insignificant because the concentration was higher in a control group. No significance was found in Cd and Hg. In the serum, on the other hand, no statistical significance was found between the heavy metal (Pb, Cd and Hg) and Alzheimer's disease. In this study, no statistical significance was observed between heavy metal and decrease in cognitive intelligence. However, it appears that a further study needs to be performed because the results of the conventional studies were inconsistent.

Evaluation of Rock Damage Zone Using Seismic Logging Method (탄성파 점층법을 이용한 암반손상대 평가)

  • Kang Seong-Seung;Hirata Atsuo;Obara Yuzo;Haraguchi Naoyuki
    • Tunnel and Underground Space
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    • 제16권1호
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    • pp.50-57
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    • 2006
  • Development of structures such as slope and tunnel, waste disposal, oil and LPG storages, and underground power house and so on, is increasing with the year. The method for appropriate estimation of rock state such as fresh or damaged rocks is also requested with increasing structural development. On these purposes, seismic logging system, which is a simple and easy way for handling as well as small and light, has been developed. Seismic logging method is one of logging tests, which is able to evaluate the state of rock mass with various shapes and is possible to obtain the relatively accuracy data at situ state. In addition, seismic logging method is at to apply to estimate structural behavior, before and after support installed. According to the results obtained from this study, firstly, it is clear that the extent of damage in rock slope due to blasting is able to be evaluated with quantity using seismic logging method, moreover to decide the damage zone in rock slope reasonably. Secondly, it is expected that installing depth of support is able to be decided more effectively and economically, using the results of seismic logging data. Finally, seismic logging method is also able to be applied safety supervision of structures, before and after support installed.

Characteristic of Local Behavior in Orthotropic Steel Deck Bridge with Open Ribs according to Running Vehicle (주행차량에 따른 개단면 강바닥판 교량의 국부거동 특성)

  • Lee, Sung-Jin;Kyung, Kab-Soo;Park, Jin-Eun;Lee, Hee-Hyun
    • Journal of Korean Society of Steel Construction
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    • 제24권1호
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    • pp.101-108
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    • 2012
  • The orthotropic steel deck bridge made by using relatively thin steel plate, and structural members such as transverse and longitudinal ribs, cross beam, etc. in the bridge are fabricated with complex shape by welding. Therefore, the possibility occurring deformation and defects by welding is very high, and stress states in the welded connection parts are very complex. Also, the fatigue cracks in orthotropic steel deck bridge are happening fromthe welded connection parts of secondary member than main member. However, stress evaluation for main members is mainly carried out in the design process of the bridge, detailed stress evaluation and characteristic analysis is not almost reviewed in the structural details which fatigue crack occurred. For the orthotropic steel deck bridge with open ribs which has been serviced for 29 years, in this study, the cause of fatigue crack is investigated and the fatigue safety of the bridge is examined based on fieldmeasurement by the loading test and real traffic condition. Also, structural analyses using gridmodel and detailed analysis model were carried out for the welded connection parts of longitudinal rib and diaphramthat fatigue crack occurred. Additionally, the behavior characteristics due to running vehicles were investigated by using influence area analysis for these structural details, and the occurrence causes of fatigue crack in the target bridge were clarified.

A Study on Evaluating Length Limit in Tangent Section of Highway Based on Driver's Workload (운전자 작업부하를 고려한 최장 허용 직선길이 결정에 관한 연구)

  • 정봉조;강정규;김주영;장명순
    • Journal of Korean Society of Transportation
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    • 제20권2호
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    • pp.17-26
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    • 2002
  • Driver's psychophysiological load is one of the key measures for evaluating the safety of the highway. The purpose of this study is to propose and to test the methodology of evaluating the length limit of tangent section using driver's psychophysiological load. Driver's psychophysiological data is represented by the data acquire by frontal and occipital lobe. In order to compare the differences between tangent segments and the orders, real road driving experiments were performed. We collected psychophysiological data during the operation of vehicles. The experimental data were analyzed using FFT (Fast Fourier Transform) and relative power spectrum tools. These routine produces the beta value which is a major factor in consideration of driver's condition. The results in this study are summarized as follows: (1) A new methodology of evaluating the length limit in tangent section of highway using driver's psychophysiological load was proposed. (2) It was observed that driver's work load at tangent section was three times lower than that at the other section types. The beta value at tangent section is 2.219, while that at general section is 0.821. (3) It was observed that the driver's work load was significantly dropped to 0.428 after the continuous driving of 4.2km tangent section. (4) Based on the experimental subjects(from 27 Years to 31). we suggest that 30 times of design speed(3.0 km) could be acceptable as the length limit of tangent section in highway rather than the Previous limit which is 20 times of design speed(2.0km).