• Title/Summary/Keyword: Safety test

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Detection of Genetically Modified Genes from Soybean Sprout Products (콩나물에서 발견된 유전자 변형 도입 유전자의 비의도적 혼입 조사)

  • 윤성철
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.3
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    • pp.227-231
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    • 2004
  • A total of 219 polymerase chain reaction tests of genetically modified (GM) DNA sequences in soybean seeds and soybean sprouts were conducted during 2000-2001. No CM gene was found in 96 tests of soybean seeds. However, either a functional CP4EPSPS gene or the 355 promoter gene was found three times in 2000 and eight times in 2001, in between 0.01 and 0.17% of soybean spout products, in 123 tests. Since the amount of GM genes was much less than the threshold limit of 3%, none of the 11 positive soybean-sprout samples needed to be libeled GM crops. Of these, seven sprout samples were from domestic seeds and four were from seeds imported from China. To find the contamination route, the raw materials, seed surface, floor of the storage room, area around the selection machine, surface of the packaging film and corn powder used in the package were tested. The 35S promoter gene was detected in only two samples of the corn powder (0.1%). Although we could not find the cause of the GM contamination, the sprout package film is one possibility. In total,8.9% of the soybean sprout tests were GM positive, but the amounts were much less than the threshold of 3%. This means that there are frequent false-positives and these would threaten the sprout industry if GMO were decided qualitatively. Food companies should make their safety data available to the public and make an effort to address people's concerns about GM food more openly. In addition, there is a need to establish a quantitative test for GM genes in sampled water and a sampling method for raw materials.

Shear Behavior of Precast Prestressed Inverted-Tee Concrete Beams with Dapped Ends (프리캐스트 프리스트레스트 콘크리트 역티형보의 댑단부 전단거동)

  • 유승룡
    • Journal of the Korea Concrete Institute
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    • v.13 no.1
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    • pp.46-53
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    • 2001
  • Two full scale precast pretensioned dapped ended rectangular beams designed by PCI design handbook for a major domestic live load of market and parking building - 500kgf/㎡ and 1,200kgf/㎡ were investigated experimentally. The bottom length of beams was 60cm which was same to the length of rectangular column in the base of five-story market or parking buildings. The height of dap was web hight plus half of the flange height within the allowable limit of PCI method. Shear tests were performed on four beam ends. Followings were obtained from the experimental study. All of the specimens were fully complied with the PCI design handbook. Two of four specimens which were designed for live load of 1,200kgf/㎡ showed crackings at the re-entrant corner of dap before the full service loadings, and failed by direct shear at the load level much less than their calculated nominal strength. The specimens designed for live load of 1,200kgf/㎡ failed at 772 tonf and 78.36tonf by direct shear crackings. This strength was less than PCI limit of 81.9 tonf and higher than ACI limit of 65.62tonf. Thus, the limit suggested by ACI seems more reasonable in regard of safety in view of this test results. According to load-strain curves, the strain of hanger reinforcement reached almost yield strain. It is recommended to use more inclined hanger reinforcement of improve the strength and serviceability.

Alkali-Activated Coal Ash(Fly Ash, Bottom Ash) Artificial Lightweight Aggregate and Its Application of Concrete (알칼리 활성화 석탄회(Fly Ash, Bottom Ash) 인공경량골재 및 콘크리트 적용)

  • Jo Byung-Wan;Park Seung-Kook;Kwon Byung-Yoon
    • Journal of the Korea Concrete Institute
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    • v.16 no.6 s.84
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    • pp.751-757
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    • 2004
  • Artificial lightweight aggregates and solids were manufactured with coal ash(fly ash, bottom ash). In order to apply alkali-activated coal ash(fly ash, bottom ash) artificial lightweight aggregate to concrete, several experimental studies were performed. Thus, it can be noticed the optimal mix proportion, basic characteristies, mechanical properties and environmental safety of alkali-activated coal ash(fly ash, bottom ash) solid and alkali-activated coal ash(fly ash, bottom ash) artificial lightweight aggregate. Also, the freezing-thawing test property of concrete using the alkali-activated coal ash(fly ash, bottom ash) artificial lightweight aggregate was investigated. As a result, the optimal mixing proportion of coal ash(fly ash, bottom ash) solid to make alkali-activated artificial lightweight aggregates was cement $10\%$, water glass $15\%$, NaOH $10\%$, $MnO_2\;5\%$. Alkali-activated coal ash(fly ash, bottom ash) solid can achieve compressive strength of 36.4 MPa, at 7-days, after the paste was cured at air curing after moist curing during 24 hours in $50^{\circ}C$. Alkali-activated coal ash(fly ash, bottom ash) artificial lightweight aggregate that do impregnation to polymer was improved $10\%$ crushing strength $150\%$, and was available to concrete.

Fatigue Behavior of Prestressed Concrete Beams Using FRP Tendons (FRP 긴장재를 이용한 프리스트레스트 콘크리트 보의 피로 거동)

  • Kim, Kyoung-Nam;Park, Sang-Yeol;Kim, Chang-Hoon
    • Journal of the Korea Concrete Institute
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    • v.23 no.2
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    • pp.135-144
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    • 2011
  • Recently, researches about fiber reinforced polymer (FRP) which has excellent durability, corrosion resistance, and tensile strength as a substitution material to steel tendon have been actively pursued. This study is performed to examine FRP tendon used prestressed beam's safety under service load. The specimen was a prestressed concrete beam with internal bonded FRP tendon. In order to compare the member fatigue capacity, a control specimen of a prestressed concrete beam with ordinary steel tendon was tested. A fatigue load was applied at a load range of 60%, 70%, and 80% of the 40% ultimate load, which was obtained though a static test. The fatigue load was applied as a 1~3 Hz sine wave with 4 point loading setup. Fatigue load with maximum 1 million cycles was applied. The specimen applied with a load ranging between 40~60% did not show a fatigue failure until 1 million cycles. However, it was found that horizontal cracks in the direction of tendons were found and bond force between the tendon and concrete was degraded as the load cycles increased. This fatigue study showed that the prestressed concrete beam using FRP tendon was safe under a fatigue load within a service load range. Fatigue strength of the specimen with FRP and steel tendon after 1 million cycles was 69.2% and 59.8% of the prestressed concrete beam's static strength, respectively.

An Experimental Study of Strength Evaluation in Frozen Soils according to Direct Shear Box Systems (직접전단상자 시스템에 따른 동결토의 강도 평가에 관한 실험적 연구)

  • Kim, Sang Yeob;Kim, YoungSeok;Lee, Jangguen;Lee, Jong-Sub
    • Journal of the Korean GEO-environmental Society
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    • v.18 no.3
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    • pp.5-14
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    • 2017
  • Experimental study on strength characteristics of frozen soils is necessary for the safety evaluation of design and construction in cold region. The objective of this study is to evaluate the direct shear strength of frozen soils obtained from traditional system (Type-1), system with roller on the upper shear box (Type-2), and system with fixed upper shear box separated from bottom shear box (Type-3). Specimens mixed with sand, silt, and water are frozen to $-5^{\circ}C$, and then direct shear tests are conducted under the normal stress of 5, 10, 25, and 50 kPa. Experimental results show that the upper shear box of Type-1 touches the bottom shear box due to the rotation of the upper shear box. The shear strength obtained from Type-2 is overestimated because the preventing rotation force is added to shear force. Type-3 may acquire the only strength of the specimen, and shear strain at peak shear strength is similar to that at the beginning of vertical displacement occurrence. In addition, internal friction angle and cohesion at both peak and residual stresses in Type-3 are smaller than those of Type-2. This study shows that high strength specimens including frozen soils can be effectively evaluated using improved shear box system such as Type-3.

Evaluation of Liquefaction Strength Based on Korean Earthquake Magnitude (국내 발생 지진규모를 고려한 액상화저항강도 산정)

  • 신윤섭;박인준;최재순;김수일
    • Journal of the Korean Geotechnical Society
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    • v.15 no.6
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    • pp.307-317
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    • 1999
  • The purpose of this study is to utilize conventional procedures for evaluation of liquefaction potential and to compare the results obtained by modified detailed method based on Korean earthquake magnitude (M=6.5). Liquefaction potential is assessed by comparing liquefaction strength of soil and cyclic shear stress generated in the soil layers during earthquakes. The cyclic shear stress is computed from the earthquake response analysis, and liquefaction strength of soil is evaluated by using results from cyclic triaxial tests. The cyclic triaxial tests are performed on many different conditions of sample ; relative densities(50%, 60%, and 70%), initial effective confining pressures (70kPa, 100kPa, and 150kPa), and fine contents(10%, 20%, and 30%). From the result of comparing the conventional procedure with the modified detailed method, it is found that the modified detailed method tends to evaluate larger safety factor against liquefaction in the weak sand site$(FS \leq1.5)$. Therefore in this case, it is suggested that liquefaction potential should be evaluated by using the modified detailed method based on cyclic triaxial tests. It is also found that in modified detailed method based on earthquake magnitude 6.5, critical depth where liquefaction can be generated is around 15m from the ground surface.

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A comparative study on the user satisfaction between two different piezoelectric engines (두가지 피에조 엔진의 사용자 만족도 비교)

  • Lim, Hyun-Mi;Lee, Kyu-Bok;Lee, Wan-Sun;Choi, So-Young
    • Journal of Dental Rehabilitation and Applied Science
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    • v.33 no.4
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    • pp.269-277
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    • 2017
  • Purpose: The aim of this study is to compare the performance of two piezoelectric engine systems by surveying satisfaction from dental clinicians. Materials and Methods: Two piezoelectric systems were evaluated: TRAUS XUS10 (Saeshin), PIEZOSURGERY touch (Mectron). For this study, 20 dentists responded to the 11 questionnaires in which 5 point Likert-type scale was used. The two devices were operated for 10 seconds and measured 5 times to compare the maximum noise values. In heat emission test, the handpiece was operated for 3 minutes and heat was measured at three positions each. Results: TRAUS XUS10 had higher satisfaction level on motor noise (P < 0.05). About function key and handpiece heat generation, PIEZOSURGERY touch showed higher satisfaction (P < 0.05) than TRAUS XUS10. The maximum noise level for each of the devices was confirmed to be 56.6 dB for the TRAUS XUS10 and 56.0 dB for PIEZOSURGERY touch. The two piezoelectric engines satisfied the safety standards with an operation temperature below $41^{\circ}C$ after having been operated for 3 minutes. Conclusion: Except for the function key and handpiece heat emission, TRAUS XUS10 has comparable performance with PIEZOSURGERY touch.

Intra-tracheal Administration of the Disinfectant Chloromethylisothiazolinone/methylisothiazolinone (CMIT/MIT) in a Mouse Model to Evaluate a Causal Association with Death (마우스의 기도 내 점적을 통한 가습기살균제 CMIT/MIT와 사망 간의 원인적 연관성에 관한 연구)

  • Kim, Hayoung;Chung, Yonghyun;Park, Yeongchul
    • Journal of Environmental Health Sciences
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    • v.43 no.4
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    • pp.247-256
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    • 2017
  • Objectives: The deaths of Korean victims exposed to the disinfectant CMIT/MIT have remained unresolved. This is mainly due to a lack of concordance between the few available toxicity tests and the abundant epidemiological data, making it difficult to establish a cause-and-effect relationship. Therefore, this study was carried out to investigate any potential associations between CMIT/MIT exposure and death. Methods: Groups of experimental and control C57BL/6 mice were instilled (in the trachea) with chloromethylisothiazolinone/methylisothiazolinone (CMIT/MIT) using a visual instillobot. CMIT/MIT was instilled over a period of three days and eight weeks, respectively, to achieve acute and chronic exposures. A threshold dose-response model was applied for estimating the threshold level as one line of evidence for a causal association between CMIT/MIT and death. Results: An acute exposure of 1.2 mg ai/kg/day of CMIT/MIT was estimated to reflect the threshold for death. The dose-response curve with this threshold showed a very steep slope and a narrow range of CMIT/MIT exposures. The narrow range of CMIT/MIT exposures, in particular, indicated an evident boundary between survival and death, thus implicating a strong causal association. A similar threshold dose-response relationship observed following acute exposure was also seen following chronic exposure to CMIT/MIT. Airborne disinfectant exposure was visible as minimal or mild lung damage with no fibrosis, as shown by histopathological tests. However, many observations are considered to be functional respiratory tract or lung failure due to death, as observed in necropsies of the mice that died due to CMIT/MIT exposures. Conclusions: There are two strong lines of evidence for a causal association between death and CMIT/MIT exposure: 1) The threshold dose-response curve, with a very steep slope and a narrow range of CMIT/MIT exposures showing a visible boundary between survival and death; and 2) many cases of functional respiratory or lung failure.

The Effects of Low-dose Electron Beam Irradiation on Quality Characteristics of Stored Apricots (저선량 전자선 조사가 살구의 저장 중 품질특성에 미치는 영향)

  • Lee, Jeong-Ok;Lee, Seong-A;Kim, Mi-Seon;Hwang, Hye-Rim;Kim, Kyoung-Hee;Chun, Jong-Pil;Yook, Hong-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.7
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    • pp.934-941
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    • 2008
  • Effect of electron beam irradiation (1 and 2 kGy) on apricot was determined in order to develop preservation techniques to enhance shelf-life during 2 weeks at room temperature. Aerobic bacteria and molds/yeasts in apricot were reduced significantly with the increase of irradiation dose. Hardness of apricots decreased during storage by irradiation. Hunter's color value results showed that lightness and redness of irradiated samples were low compared with control samples. Also, sensory test resulting overall acceptability was not significantly different by 1 kGy irradiation during the storage days. Reducing sugar contents was increased gradually, and value of irradiated samples was high compared with non-irradiated sample. Organic acid contents of 2 kGy irradiated samples was not significantly changed during storage. In pH, total sugar, hydrogen donating activity and vitamin C contents, there were no significant differences between treatments. The electron beam treatment on apricots at 1 and 2 kGy did not affect pH, total sugar, hydrogen donating activity and vitamin C contents but improved microbial safety.

Processing and Fermentation of Food Wastes with MS Microorganism Complex for Swine Feeds (MS 미생물복합군에 의한 음식폐기물의 양돈사료화를 위한 적정처리공정)

  • Lee, Jeong-Chae;Jung, Woo-Jin;Lim, Kye-Taek;Kim, Tae-Hwan
    • Korean Journal of Environmental Agriculture
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    • v.19 no.4
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    • pp.270-275
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    • 2000
  • In order to investigate the proper processing of food wastes with miraculous soil-microorganisms (MS) for final use of swine feeds, calory, amino acid and fatty acid in food wastes were determined in relation with fermentation process with MS microorganism complex. Aflatoxin test was also performed to check safety of the fermented food wastes. Calory of food wastes was determined in average $7.60\;Kcal{\cdot}g^{-1}\;D.W.$ In finally processed food wastes, total content of amino acid was $93.0\;mg{\cdot}g^{-1}]\;D.W$, showing 18.5% of increase by the anaerobic fermentation. Essential and non-essential amino acids were measured at respectively 34.43 and $58.56\;mg{\cdot}g^{-1}\;D.W.$ Leucine, phenylalanine, isoleucine and threonine of essential amino acids and proline and glutamic acid of non-essential amino acids were highly composed as compared to others. The composition of fatty acid in food wastes was also increased by anaerobic fermentation for 3 weeks. Palmitic acid, oleic acid and palmitoleic acid were more important in quantity. Present results indicate that food wastes properly processed with MS have enough calory and are safe from aflatoxin, and that anaerobic fermentation with MS microorganism in an efficient process for hydrolyzing protein and lipids in food wastes.

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