• Food Science of Animal Resources
• /
• v.23 no.3
• /
• pp.269-277
• /
• 2003

This study was carried out to evaluate the safety of Korean human milk. The microorganisms were identified from human milk of 149 healthy mothers by two collection methods, hand and pump expression. The means of total bacterial counts were 2.33x10$^4$ cfu/mL on the samples collected by the pump expression and 7.83xl0$^3$ cfu/mL on those collected by the hand expression. Therefore, the total bacterial counts of pump expression samples was 9.80xl0$^2$∼3.06x10$^4$ cfu/mL more than that of hand expression samples. The coliform counts of pump expression was 9.36xl0$^3$∼8.57xl0$^4$ cfu/mL more than that of hand expression. However, there was any significant differences of the lactic acid bacterial counts between the two samples collected by each methods. 100 strains of 5 patterns of total bacterial counts were isolated based on the morphology of colony in the standard plate count agar. 13 species were identified among the isolated strains. The dominant species in Korean human milk were Staphylococcus which 7 subspecies identified(81% in the rate of total bacteria, 1.07x10$^4$ cfu/mL). Other species identified were Micrococcus, Bacillus, Providencia, Pseudomonas, Yersinia and Acinetobacter. 36 strains of 6 patterns of lactic acid bacterial counts were isolated based on morphology of colony in the BCP agar. 7 species were identified among the isolated strains. The dominant species of lactic acid bacteria in Korean human milk were Lactobacillus brevis(50.9% in the rate of lactic acid bacteria, 4.72xl0$^4$ cfu/mL). Others species identified(49.1% lactic acid bacteria) were Lactobacillus curvatus, Lactobacillus delbrueckii subsp. lactis, Lactobacillus acidophilus, Leuconostic lactis and Streptococcus salivarius subsp. thermophilus.

• Journal of agricultural medicine and community health
• /
• v.29 no.2
• /
• pp.303-314
• /
• 2004

Objectives: This study was carried out to assess exposure levels of organic dusts and ammonia in poultry farms in Korea. Methods: A total of six poultry farms were investigated. The farms were located in Namwon, Chonlabuk-do and in Kae-San, Chungchongbuk-do. This study consisted of a questionnaire and measuring organic dusts and ammonia. The questionnaire included the characteristics of the farms, work patterns and the tasks of the poultry farms. Results and Conclusions: The farmers raised the chickens 45 times a year and the average number of years in the poultry farm were eight years ranging from 2 to 12 years. They worked for seven days per week and the average hours spent caring the chickens are 6.3 hours per day. The duration of staying in the confinement buildings was 3.3 hours per day. The work time in summer was longest. The feed and the water supply systems were automatic and the control of ventilation windows used "winch curtain" was semiautomatic. They used mechanical ventilation system in winter and used dilution ventilation system in the other seasons. The geometric mean concentration of total and respirable dust sampled in the poultry confinement buildings was 4.0 mg/$m^3$and 0.9 mg/$m^3$ respectively. The ratio of respirable to total dusts range from 9 to 49 percent. There was no sample exceeding the criteria 10 mg/$m^3$ for total dust and 3 mg/$m^3$ for respirable dust in farms. The criteria have been recommended by Korean Ministry of Labor and American Conference of Governmental Industrial Hygienist. The personal respirable dusts measured during a circle work averaged geometric mean concentration 1.4 mg/$m^3$ Two personal samples were exceeded the threshold 3 mg/$m^3$. There was a positive relation between an index and the personal samples of respirable dusts($R^2$=0.98). The index is calculated by multipling the total number of chickens in the farm by the age of the chickens and then dividing by the volume of the confinement building. The geometric mean concentration of area and personal ammonia samples was 23.3 ppm and 22.2 ppm, respectively. Some of the ammonia samples, both area and personal samples, exceeded the short term exposure limit value 35 ppm.

• Journal of Life Science
• /
• v.22 no.9
• /
• pp.1152-1158
• /
• 2012

Development of transgenic plant increasing crop yield or disease resistance is good way to solve the world food shortage. However, the persistence of marker genes in crops leads to serious public concerns about the safety of transgenic crops. In the present paper, we developed marker-free transgenic rice inserted high molecular-weight glutenin subunit (HMW-GS) gene ($D{\times}5$) from the Korean wheat cultivar 'Jokyeong' using Agrobacterium-mediated co-transformation method. Two expression cassettes comprised of separate DNA fragments containing only the $D{\times}5$ and hygromycin resistance (HPTII) genes were introduced separately into Agrobacterium tumefaciens EHA105 strain for co-infection. Each EHA105 strain harboring $D{\times}5$ or HPTII was infected into rice calli at a 3: 1 ratio of EHA105 with $D{\times}5$ gene and EHA105 with HPTII gene expressing cassette. Then, among 66 hygromycin-resistant transformants, we obtained two transgenic lines inserted with both the $D{\times}5$ and HPTII genes into the rice genome. We reconfirmed integration of the $D{\times}5$ and HPTII genes into the rice genome by Southern blot analysis. Wheat $D{\times}5$ transcripts in $T_1$ rice seeds were examined with semi-quantitative RT-PCR. Finally, the marker-free plants containing only the $D{\times}5$ gene were successfully screened at the $T_1$ generation. These results show that a co-infection system with two expression cassettes could be an efficient strategy to generate marker-free transgenic rice plants.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.55 no.2
• /
• pp.144-150
• /
• 2010

The agricultural techniques of environmental friendly using the green manure crops have been required recently more to have the safety agricultural products and to reduce the use of fertilizers and agricultural chemicals. The utilization of green manure crops related closely to cropping system would be very important. The purposes of this study are both to investigate the effects of green manure crops of hairy vetch, legume, and rye, gramineae, and to compare the effects between them in rice cultivation. The hairy vetch and rye were treated as green manure crops into paddy soil at 10 days before the rice transplanting. The plant height was increased gradually from the maximum tillering to the heading growth stage, showing that it was the highest with treatment of conventional cultivation in 2007 and the highest with hairy vetch in 2008. The number of tillers was higher with treatment of hairy vetch and hairy vetch+rye than those with the conventional cultivation. Dry weight was also higher with hairy vetch than that with the conventional, while it was lower clearly in rye than those in hairy vetch and conventional. According to the high tilller number and spikelet number per panicle out of the yield components relatively, the yield of rough rice was increased to about 6% and 8% in 2007 and 2008, respectively, comparing with the yield in the conventional cultivation. Based on these results, the hairy vetch would be a good green manure crop in rice cultivation.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.39 no.10
• /
• pp.1545-1554
• /
• 2010

Korean government will set up the nationwide food safety system with strict control of hazardous nutrients like sugar, fatty acids and sodium as well as advanced nutrition education system. In addition, almost one hundred percent of school food service rate forced the government to consider more effective ways to upgrade the nutritional status of school meals. The object of our study was to provide the data on content and consumption of sugar in school meal for the nationwide project. For this purpose, we surveyed the sugar content of 842 school meal menus and their intake level for 154 days in 8 schools in Daejeon and Chungcheong Province. Sugar contents, the sum of the quantity of 5 sugars commonly detected in food, were analysed with HPLC-RID (Refractive Index Detector). Sugar intakes were calculated by multiplying the intake of each menu to the sugar content of that menu. The sugar content was highest in the desserts, which include fruit juices, dairy products and fruits. Sugar content of side dish was high in sauces and braised foods. Sugar intake from one dish is high in beverage and dairy product, and one dish meals contribute greatly to sugar intake because of their large amount of meal intake. The average lunch meal intakes of second grade and fifth grade elementary school students were 244 g/meal and 304 g/meal, respectively. The meal intake of middle school student was 401 g/meal. The average sugar intake from one day school lunch was 4.22 g (4.03 g on elementary and 5.31 g on middle school student), which is less than 10% of daily sugar reference value for Koreans. The result of this study provides exact data of sugar intake pattern based on the content of sugar which is matched directly to the meals consumed by the students.

• Journal of radiological science and technology
• /
• v.36 no.2
• /
• pp.123-129
• /
• 2013

This study investigated the rate of setup variance by the rotating unbalance of gantry in image-guided radiation therapy. The equipments used linear accelerator(Elekta Synergy TM, UK) and a three-dimensional volume imaging mode(3D Volume View) in cone beam computed tomography(CBCT) system. 2D images obtained by rotating $360^{\circ}$and $180^{\circ}$ were reconstructed to 3D image. Catpan503 phantom and homogeneous phantom were used to measure the setup errors. Ball-bearing phantom was used to check the rotation axis of the CBCT. The volume image from CBCT using Catphan503 phantom and homogeneous phantom were analyzed and compared to images from conventional CT in the six dimensional view(X, Y, Z, Roll, Pitch, and Yaw). The variance ratio of setup error were difference in X 0.6 mm, Y 0.5 mm Z 0.5 mm when the gantry rotated $360^{\circ}$ in orthogonal coordinate. whereas rotated $180^{\circ}$, the error measured 0.9 mm, 0.2 mm, 0.3 mm in X, Y, Z respectively. In the rotating coordinates, the more increased the rotating unbalance, the more raised average ratio of setup errors. The resolution of CBCT images showed 2 level of difference in the table recommended. CBCT had a good agreement compared to each recommended values which is the mechanical safety, geometry accuracy and image quality. The rotating unbalance of gentry vary hardly in orthogonal coordinate. However, in rotating coordinate of gantry exceeded the ${\pm}1^{\circ}$ of recommended value. Therefore, when we do sophisticated radiation therapy six dimensional correction is needed.

• Korean Security Journal
• /
• no.29
• /
• pp.163-191
• /
• 2011

Recently, the problems in school violence did not stop on the crime between the members at the school and which developed into the invasion crime of the school caused by outsiders. The school is no more the safety zone from the crime. Particularly, in the case of the elementary school, because there are nearly no people who oppose to the outside attacker and can control this, it is the place where it is vulnerable to the invasion crime. The Metropolis of Seoul implements the School Sheriff system within the jurisdiction bureau, in the public elementary school. However, actually the School Sheriff business is being managed, never applying a rule in the Security Industry Law with the main content, that is the Security Industry Law application is excluded. Because the jurisdiction on the contract of Seoul City and operating company are run, the various issues is caused. First, since it is not being considered as a security business, the commercial liability insurance for security company has no chance to applicate when the operation company and the School Sheriff have related damage generation. So the security for the indemnification of loss of the victim is weak. Second, The task of the School Sheriff is ruled just by in the individual contracts. But it is insufficient with this thing. The related duties are required some supplement like a general rule application including the obligation of the guard in the security industry law. Third, the education of the School Sheriff needs to connect with the educational programme in the security industry law. The related professional education specially needed for the prevention of school violence ought to be reserved compensation. Forth, the citizens still demand the strengthening of police patrol for the surroundings of a school in spite of the result of Seoul City's public survey. Therefore, the active relation of cooperation with the police needs to be supported legally and institutionally with the Security Industry Law application. Fifthly, the success of the School Sheriff business can be more guaranteed with the supervision of the legal and institutional device like a the Security Industry Law application or police and all sorts of administrative execution's and etc.

• Journal of agriculture & life science
• /
• v.45 no.6
• /
• pp.163-173
• /
• 2011

The objective of this study was to determine microbiological risk factors in hot pepper farms for the application of good agricultural practices (GAP). Samples were collected from cultivation environments and utensils, plants, workers, and air at 3 hot pepper farms located in Cheongsong, Korea and were tested to detect sanitary indications [aerobic plate bacteria (APC), coliform, and Escherichia coli], foodborne pathogens, and fungi. APC, coliform, and fungi were detected at the levels of 0.7~6.2, 0.2~4.7, and 0.4~4.3 log CFU, respectively, in the three farms. Four (4.4%; l leaf, l irrigation water, and 2 soil) of 90 samples collected were revealed to be E. coli positives. For foodborne pathogens, Staphylococcus aureus was only detected at $1.0log\;CFU/100cm^2$ in the worker's cloth of B farm, and Bacillus cereus was detected at the levels 1.0~2.5 log CFU in the cultivation environments and utensils and worker of B and C farms. However, other pathogens were not detected. The results demonstrated potential microbiological risks for hot pepper cultivated in the farms. Therefore, a management system to minimize the microbial risk such as GAP is required to ensure the safety of hot pepper.

• Journal of Climate Change Research
• /
• v.5 no.1
• /
• pp.13-19
• /
• 2014

In the past, Korea agro-climatic zone except Jeju-do was classified into nineteen based on rice culture by using air temperature, precipitation, and sunshine duration etc. during rice growing periods. It has been used for selecting safety zone of rice cultivation and countermeasures to meteorological disasters. In this study, the climatic variables such as air temperature, precipitation, and sunshine duration of twenty agro-climatic zones including Jeju-do were compared decennially (1970's, 1980's, 1990's, and 2000's). The meteorological data were obtained in Meteorological Information Portal Service System-Disaster Prevention, Korea Meteorological Administration. The temperature of 1970s, 1980s, 1990s, and 2000s were $12.0{\pm}0.14^{\circ}C$, $11.9{\pm}0.13^{\circ}C$, $12.2{\pm}0.14^{\circ}C$, and $12.6{\pm}0.13^{\circ}C$, respectively. The precipitation of 1970s, 1980s, 1990s, and 2000s were $1,270.3{\pm}20.05mm$, $1,343.0{\pm}26.01mm$, $1,350.6{\pm}27.13mm$, and $1,416.8{\pm}24.87mm$, respectively. And the sunshine duration of 1970s, 1980s, 1990s, and 2000s were $421.7{\pm}18.37hours$, $2,352.4{\pm}15.01hours$, $2,196.3{\pm}12.32hours$, and $2,146.8{\pm}15.37hours$, respectively. The temperature in Middle-Inland zone ($+1.2^{\circ}C$) and Eastern-Southern zone ($+1.1^{\circ}C$) remarkably increased. The temperature increased most in Taebak highly Cold zone ($+364mm$) and Taebak moderately Cold Zone ($+326mm$). The sunshine duration decreased most in Middle-Inland Zone (-995 hours). The temperature (F=2.708, df=3, p= 0.046) and precipitation (F=5.037, df=3, p=0.002) increased significantly among seasons while the sunshine duration decreased significantly(F=26.181, df=3, p<0.0001) among seasons. In further study, it will need to reclassify agro-climatic zone of rice and it will need to conduct studies on safe cropping season, growth and developing of rice, and cultivation management system etc. based on reclassified agro-climatic zone.

• Korean Journal of Remote Sensing
• /
• v.34 no.6_1
• /
• pp.1041-1053
• /
• 2018

In this study, a real-time storage level and capacity monitoring and forecasting system for Yongdam Dam watershed was developed using high resolution satellite image. The drought indices such as Standardized Precipitation Index (SPI) from satellite data were used for storage level monitoring in case of drought. Moreover, to predict storage volume we used a statistical method based on Principle Component Analysis (PCA) of Singular Spectrum Analysis (SSA). According to this study, correlation coefficient between storage level and SPI (3) was highly calculated with CC=0.78, and the monitoring and predictability of storage level was diagnosed using the drought index calculated from satellite data. As a result of analysis of principal component analysis by SSA, correlation between SPI (3) and each Reconstructed Components (RCs) data were highly correlated with CC=0.87 to 0.99. And also, the correlations of RC data with Normalized Water Surface Level (N-W.S.L.) were confirmed that has highly correlated with CC=0.83 to 0.97. In terms of high resolution satellite image we developed a water detection algorithm by applying an exponential method to monitor the change of storage level by using Multi-Spectral Instrument (MSI) sensor of Sentinel-2 satellite. The materials of satellite image for water surface area detection in Yongdam dam watershed was considered from 2016 to 2018, respectively. Based on this, we proposed the possibility of real-time drought monitoring system using high resolution water surface area detection by Sentinel-2 satellite image. The results of this study can be applied to estimate of the reservoir volume calculated from various satellite observations, which can be used for monitoring and estimating hydrological droughts in an unmeasured area.