• 한국안전학회지
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• 제29권4호
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• pp.153-159
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• 2014

Low back pain is one of major issues in musculoskeletal diseases mainly caused by MMH (Manual Material Handling) tasks. In Korea, the standards of NIOSH(National Institute for Occupational Safety and Health) Lift Equations in U. S. A. have been most widely used. However, there is no standard in case the height of one feet is higher than that of another one. Moreover, since the standards are developed in U. S. A., there are many limitations for the applicability of Korean workers. In this study, MVC(Maximum Voluntary Contraction) for four postures are measured and an empirical model based on SVR(Support Vector Regression) is constructed. Constructing SVR model, PSO(Particle Swarm Optimization) is employed to investigate the optimal parameters of SVR. The results show that the performance of this empirical model is approximately accurate, even if the deviation of experimental values is large due to the individual differences. This empirical model may contribute to establish the standards of MMH tasks in Korea.

• 생태와환경
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• 제52권4호
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• pp.394-402
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• 2019

The Agrobacterium tumefaciens mediated gene transfer is widely used to generate genetic transformation of plants and transient assay of temporal exogenous gene expression. Syringe infiltration system into tobacco (Nicotiana benthamiana) leaves is a powerful tool for transient expression of target protein to study protein localization, protein-protein binding and protein production. However, the protocol and technical information of transient gene expression, especially double strand RNA (dsRNA), in tobacco using Agrobacterium is not well known. Recently, dsRNA is crucial for insecticidal effect on destructive agronomic pest such as Corn rootworm. In this study, we investigated the factor influencing the dsRNA expression efficiency of syringe agro-infiltration in tobacco. To search the best combination for dsRNA transient expression in tobacco, applied two Agrobacterium cell lines and three plant vector systems. The efficiency of dsRNA expression has estimated by real-time PCR and digital PCR. As a result, pHellsgate12 vector constructs showed the most effective accumulation of dsRNA in the cell. These results indicated that the efficiency of dsRNA expression was depending on the kind of vector rather than Agrobacterium cells. In summary, the optimized combination of transient dsRNA expression system in tobacco might be useful to in vivo dsRNA expression for functional study and risk assessment of dsRNA.

• Nuclear Engineering and Technology
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• 제53권1호
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• pp.148-163
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• 2021

Timely fault identification is important for safe and reliable operation of the electric valve system. Many research works have utilized different data-driven approach for fault diagnosis in complex systems. However, they do not consider specific characteristics of critical control components such as electric valves. This work presents an integrated shallow-deep fault diagnostic model, developed based on signals extracted from DN50 electric valve. First, the local optimal issue of particle swarm optimization algorithm is solved by optimizing the weight search capability, the particle speed, and position update strategy. Then, to develop a shallow diagnostic model, the modified particle swarm algorithm is combined with support vector machine to form a hybrid improved particle swarm-support vector machine (IPs-SVM). To decouple the influence of the background noise, the wavelet packet transform method is used to reconstruct the vibration signal. Thereafter, the IPs-SVM is used to classify phase imbalance and damaged valve faults, and the performance was evaluated against other models developed using the conventional SVM and particle swarm optimized SVM. Secondly, three different deep belief network (DBN) models are developed, using different acoustic signal structures: raw signal, wavelet transformed signal and time-series (sequential) signal. The models are developed to estimate internal leakage sizes in the electric valve. The predictive performance of the DBN and the evaluation results of the proposed IPs-SVM are also presented in this paper.

• Toxicological Research
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• 제20권2호
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• pp.109-116
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• 2004

For the safety evaluation of adenovirus-mediated gene transfer, we investigated differential gene expressions after transfecting adenoviral vector containing p16 tumor suppressor gene (Ad5CMV-p16) into human non-small cell lung cancer cells. In the previous study, we showed adenovirus-mediated $p16^{INK4a}$ gene transfer resulted in significant inhibition of cancer cell growth. We investigated gene expression changes after transfecting Ad5CMV-p16, Ad5CMV (null type, a mock vector) into A549 cells by using cDNA chip and oligonucleotide microarray chip (1200 genes) which carries genes related with signal transduction pathways, cell cycle regulations, oncogenes and tumor suppressor genes. We found that $p16^{INK4a}$ gene transfer down regulated 5 genes (cdc2, cyclin D3, cyclin B, cyclin E, cdk2) among 26 genes involved in cell cycle regulations. Compared with serum-free medium treated cells, Ad5CMV-p16 changed 27 gene expressions, two fold or more on oligonucleotide chip. In addition, Ad5CMV-p16 did not seem to increase the tumorigenicity-related gene expression in A549 cells. Further studies will be needed to investigate the effect of Ad5CMV-p16 on normal human cells and tissues for safety evaluation.

• 대한안전경영과학회지
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• 제14권3호
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• pp.269-276
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• 2012

플라스틱 사출 제품은 다양한 가전제품과 하이테크 제품에 널리 사용되고 있다. 그러나 현재의 치열한 경쟁적 비즈니스 환경에서 플라스틱 사출 제품 제조업자들은 고객을 만족시키면서 경쟁력을 얻기 위하여 다른 경쟁자들보다 먼저 새로운 제품을 시장에 출시하고 신제품의 개발기간을 줄이기 위한 노력을 할 여유가 부족하다. 따라서 무한경쟁의 시장에서 살아남기 위해서는 제조업자들은 시장 마켓 점유를 빠르게 올리는 것과 동시에 제품의 가격 경쟁력을 가져야 한다. 특징기반 모델의 구조는 현재 연구에서 3D 제작 도구로서 일반적으로 적용되고 있으며 신제품 개발 엔지니어들이 새로운 제품의 개념을 개발하는 데에도 널리 사용되고 있다. 본 연구에서는 특징기반 플라스틱 사출제품을 위한 유전자 알고리즘과 Support Vector Regression (SVR) 기반의 새로운 하이브리드 비용 평가 모델을 제안한다. 제안하는 하이브리드 모델은 기존의 플라스틱 사출제품의 비용평가절차와 계산을 위해 필요로 하는 변수들을 극적으로 간단하게 하고 줄일 수 있다. 사례연구에서는 제안하는 하이브리드 모델과 기존의 multilayer perceptron networks (MLP) 및 pure SVR과의 비교분석을 통하여 제안모델이 플라스틱 사출 제품의 개발단계에서의 비용평가문제를 해결하는데 효율성과 효과성이 있음을 입증한다.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2015년도 춘계학술대회
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• pp.954-957
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• 2015

polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) 유전자로 구성된 재조합 베큘로바이러스를 제작하였다. 본 재조합 베큘로바이러스 시스템은 여러가지 세포주와 여러 가지 조직에 감염하여 시험하였고 재조합된 유전자의 전달과 유전자 발현을 대조 벡터시스템과 비교하였다. 본 연구의 결과로 제작된 재조합 베큘로바이러스 시스템은 유전자의 전달과 발현에 있어서 대조 벡터시스템 보다 효능과 안전성면에서 우수하였다.

• 터널과지하공간
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• 제6권2호
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• pp.166-174
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• 1996

Stability of rock slope is greatly affected by the geometry and strength of discontinuities developed in the rock mass. In this study an analytical method which is capable of analyzing the effect of relative orientation between the discontinuities and the slope face on the safety of slope by assessing their vector components was used to evaluate the stability and the maximum cut-angle for the proposed slope design. The results of computerized vector analysis revealed that slope area under investigation might be divided into 3 sections of different face directions. The safety factors for benches in each 3 sections were calculated using the limit-equilibrium theory. Then, by utilizing the concept of probabilistic risk analysis, the susceptibility of entire slope failure was estimated. Based on the distribution of safety factor in each bench, the maximum cut angle of each section could be selected differently ot achieve the permanent stability of the entire slope.

• 로봇학회논문지
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• 제5권2호
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• pp.127-134
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• 2010

In this paper, a hybrid semi-3D path planning algorithm combining Virtual Tangential Vector(VTV) and fuzzy control is proposed. 3D dynamic environmental factors are reflected to the 2D path planning model, VTV. As a result, the robot can control direction from 2D path planning algorithm VTV and speed as well depending on the fuzzy inputs such as the distance between the robot and obstacle, roughness and slope. Performances and feasibilities of the suggested method are demonstrated by using Matlab simulations. Simulation results show that fuzzy rules and obstacle avoidance methods are working properly toward virtual 3D environments. The proposed hybrid semi-3D path planning is expected to be well applicable to a real life environment, considering its simplicity and realistic nature of the dynamic factors included.

• 해양환경안전학회지
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• 제30권4호
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• pp.317-323
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• 2024

STCW 협약 A-VI에 의거, 승선 필수 증서를 발급받기 위해 소화 과정 최저 훈련 이수를 거치게 되는 공간이 수소화 훈련장이다. 선박 화재의 상황과 유사한 장소에서 발생한 화재 진압 훈련을 위한 실습 장소의 특성상, 재실자의 안전을 보장할 수 있는 운영을 위해 안전성을 수치화하여 평가하고 기준을 수립하는 연구의 필요성이 대두되었다. 화재 안전 평가를 위해 FDS를 기반으로 한 Pyrosim을 활용하여 제연설비 유무에 따른 Case를 설정, vector의 분석을 통한 연기 유동 및 열기에 대한 평가를 수행하였다. 피난 안전평가는 Pathfinder를 통해 허용 피난시간, 총 피난시간을 수치화된 결과로 해석, 비교하여 안전성을 분석하였다. 각 Case에 대한 안전성을 평가함으로 제연설비별 기능의 적정성을 수치, 시각적으로 나타내었으며, 현재 운영 상태는 안전성이 양호한 것으로 평가하였다. 집진설비가 정지한 비상상황은 각 피난시간과 111.2초의 여유시간으로 나타내어 수소화 훈련장의 비상상황에 대한 피난시간의 기준으로 활용할 것을 제시하였다.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 1994년도 가을 학술발표회 논문집
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• pp.145-151
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• 1994

Welded connections have been designed on basis of allowable stresses, wherein the response to loading is assumed to be totally elastic. This is the vector analysis method, which resolves the stresses determined from the direct stress formula and the torsion formula into a vector combination to obtain a solution. It has been known that this method gives conservative answers and typically a very high factor of safety. An analytical method based on the Instantaneous Center of Rotation has been developed which predicts the ultimate strength of an eccentically loaded fillet welded connection. The method of Instantaneous Center of Rotation results in weld resistance capacities greater than the vector analysis method, by recognizing the variation in fillet weld strength with respect to the direction of the applied loading and actual load-deformation response of elemental fillet welds. The procedure of numerical analysis is iterative and complex. The relations between vector analysis method and the method of Instantaneous Center of Rotation on eccentrical distance subjected to variation of load direction are presented in this paper. Considering of the effects on configuration of weld groups, the method of Instantaneous Center of Rotation are provided a more exact results of the numerical analysis.