• 대한교통학회지
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• 제33권6호
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• pp.543-553
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• 2015

본 연구는 대중교통 특히, 버스의 이용과정에서 이용자가 받는 외부 자극(이용환경)이 스트레스(육체적 정신적 부하)에 미치는 영향관계를 분석하였다. 이를 위해 요인분석 및 구조방정식모형(Structural Equation Model)을 이용하였으며, 대전 시내버스 이용자를 대상으로 자료를 수집하였다. 분석결과, 5개 변수 중 탑승혼잡요인이 버스 이용자의 스트레스에 가장 큰 영향을 주는 요인인 것으로 나타났으며(${\beta}=.416$), 다음으로 환승요인(${\beta}=.392$), 운영요인(${\beta}=.265$), 계절요인(${\beta}=.150$), 정류장시설요인(${\beta}=.090$)의 순으로 나타났다. 즉, 이용자의 스트레스는 시설과 운영의 양적인 요인보다는 질적인 서비스 수준 더 큰 관련성이 있는 것으로 분석된 바, 버스정책은 이용자 중심으로 전환되어야 할 것이다. 또한, 스트레스는 이용자특성(성별, 연령, 정기이용여부)에 따라 다르게 나타나므로 보다 세분화된 서비스 제고전략이 유효할 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제31권2호
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• pp.218-224
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• 2018

Objective: This study evaluated the effects of different levels of protein concentrate supplementation on the growth performance of yak calves, and correlated the growth rate to changes occurring in the plasma- amino acids, -insulin profile, and signaling activity of mammalian target of rapamycin (mTOR) cascade to characterize the mechanism through which the protein synthesis can be improved in early weaned yaks. Methods: For this study, 48 early (3 months old) weaned yak calves were selected, and assigned into four dietary treatments according to randomized complete block design. The four blocks were balanced for body weight and sex. The yaks were either grazed on natural pasture (control diet) in a single herd or the grazing yaks was supplemented with one of the three protein rich supplements containing low (17%; LP), medium (19%; MP), or high (21%; HP) levels of crude proteins for a period of 30 days. Results: Results showed that the average daily gain of calves increased (0.14 vs 0.23-0.26 kg; p<0.05) with protein concentrates supplementation. The concentration of plasma methionine increased (p<0.05; 8.6 vs $10.1-12.4{\mu}mol/L$), while those of serine and tyrosine did not change (p>0.05) when the grazing calves were supplemented with protein concentrates. Compared to control diet, the insulin level of calves increased (p<0.05; 1.86 vs $2.16-2.54{\mu}IU/mL$) with supplementation of protein concentrates. Addition of protein concentrates up-regulated (p<0.05) expression of mTOR-raptor, mammalian vacuolar protein sorting 34 homolog, the translational regulators eukaryotic translation initiation factor 4E binding protein 1, and S6 kinase 1 genes in both Longissimus dorsi and semitendinosus. In contrast, the expression of sequestosome 1 was down-regulated in the concentrate supplemented calves. Conclusion: Our results show that protein supplementation improves the growth performance of early weaned yak calves, and that plasma methionine and insulin concentrations were the key mediator for gene expression and protein deposition in the muscles.

• Spatial Information Research
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• 제21권6호
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• pp.43-55
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• 2013

최근 실내 공간에서의 재난, 화재와 테러 등 대피상황을 재현하여 이를 가시화하기 위한 연구가 주목 받고 있으며, 실내 공간에 대한 모델을 설계하고 인명 안전 평가를 통한 신뢰성 있는 분석이 요구되고 있다. 이에 본 연구에서는 실제적인 건물 화재 위험 요인을 고려하여 피난 안전성 분석과 피난 경로 안내가 가능한 시뮬레이션 모델을 개발하고자 하였다. 이를 위해 인천터미널역 역사를 대상으로 3차원 화재 및 피난 모델을 설계하고, 실내 내장재의 재질을 바탕으로 열 매개변수와 화재 인지 장치를 이용하여 화재 위험 분석을 수행하였다. 둘째, 인명안전을 위한 평가에 있어 화재 시뮬레이션인 FDS(Fire Dynamics Simulator)와 피난 시뮬레이션을 통해 재실자가 인체에 손상 없이 견딜 수 있는 피난허용시간(ASET: Available Safe Egress Time)을 산출하였다. 또한 화재를 감지하고 안전한 장소까지 완전하게 피난하는데 소요되는 피난요구시간(RSET: Required Safe Egress Time)을 계산하고 이를 비교 분석하였다. 결과적으로 연구대상의 3차원 공간적인 정보를 기반으로 한 실내 공간 모델과, 고시된 안전기준을 반영한 열차 내 화재 및 피난 위험도 측정 시뮬레이션 분석을 통해 보다 실제적인 안전성 평가를 수행 할 수 있었다.

• 한국자원식물학회지
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• 제36권1호
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• pp.100-106
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• 2023

과수 화상병을 방제하기 위해 발병 초기에 과수원 반경 내 전체 식물을 제거하거나 예방을 위해 약제 방제를 시행하고 있다. 과수에 대한 항생제 사용이 시작된 이후, 항생제 저항성 병원균 발생 가능성과 의도하지 않은 수체 및환경에 대한 해로운 영향이 문제가 되고 있다. 본 연구의 목적은 화상병 방제약제 과다 살포로 인한 과수의 피해 정도를 확인하고, 안전한 약제 방제 기준을 설정하기 위해 수행하였다. 과수 생육기 동안 안전 사용 횟수를 초과하여 3종의 과수 화상병 방제약제를 지속해서 살포하 였을 때 발생하는 약해와 처리한 과실에서 약제 잔류허용기준 초과 여부를 분석하였다. 사과 '후지'와 배 '신고'에서 약해는 보이지 않았고, 무대재배를 한 '후지'에서 oxolinic acid가 정량한계를 넘어서 검출되었고, 나머지 약제 성분은 잔류허용기준 이하로 검출되었다. 안전 사용 횟수를 초과하여 방제약제를 사용하게 되면 약제 성분이 과실에 남을 수 있으므로 정해진 희석배수를 지키고 안전 사용 횟수와 함께 사용 적기를 반드시 준수해야 함을 알 수 있다.

• 농업과학연구
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• 제45권2호
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• pp.283-289
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• 2018

Agricultural gear reducers are used in a variety of agricultural machinery designs such as in agricultural tractors and transport cars, and even greenhouses. For greenhouses, a gear reducer is used to control windows on the side and the roof. Gear reducers for agricultural applications are designed using the empirical method because of the lack of a standard for experimentation. Simulation is necessary for the optimal design of an agricultural gear reducer. There are many advantages to this optimization such as low-cost maintenance, reduced size, and weight. In this study, bending and contact safety factor simulation for the gear reducer of a greenhouse was conducted by decreasing the face widths of helical gear shaft 2 and shaft 3 from 30.8 and 30 mm, respectively, at an interval of 4 mm. The bending and contact safety factors were calculated using AGMA standard. Simulation results showed that bending and contact safety factors decreased rapidly when the face width of the helical gear on shaft 2 was 30 mm and the face width of helical gear on shaft 3 decreased from 30.8 mm to 26.8 mm, suggesting that it would be safe to reduce the face width of the helical gear on shaft 3 to 26.8 mm. The reduction of the face width also reduced the weight of the agricultural gear. This study suggests that the agricultural gear reducer safety factor decreases as the face width decreases.

• 교육녹색환경연구
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• 제12권3호
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• pp.58-67
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• 2013

This study is to analyze the satisfaction and the image evaluation of landscape elements in outdoor space by types of the university campus. The results are as follows. 1) Out of outdoor elements at university campus, planting area, resting area, access road, and water feature are recognized as major landscape elements. Among them, planting area and access roads are evaluated low in terms of satisfaction levels, therefore, improvement on these elements are required. 2) In outdoor space image evaluation, university campus has image such as 'simple', 'clear', and 'safe'. By scale of universities, both 'A' university, which is the biggest in terms of size of campus, and 'B' university, which has a medium sized campus, have a positive image. However, 'C' university, which is the smallest in terms of size of campus, has a passive and negative image. 3) 6 factors are extracted through Factor Analysis for image evaluation. All of the universities show positive image in the categories of 'clarity' and 'familiarity', however, 'B' university and 'C' university show negative image in the category of 'scale'. 4) In Correlation Analysis between landscape elements satisfaction level and image evaluation, it is showed that the group of landscape facility becomes a relation factor of overall image evaluation. As a result, the higher satisfaction level goes, the better image evaluation of overall outdoor space at university campus is.

• Asian Pacific Journal of Cancer Prevention
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• 제16권5호
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• pp.2031-2035
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• 2015

Intra-tumoral hypoxia is an environment that promotes tumor cell migration, angiogenesis and epithelial-mesenchymal transition that accounts for a major mechanism of metastasis. Chloroquine potentially offers a new therapeutic approach with an 'old' drug for effective and safe cancer therapies, as it exerts anti-metastatic activity. We investigated the inhibitory effect of chloroquine on cholangiocarcinoma (CCA) cell migration under cobalt chloride ($CoCl_2$)-stimulated hypoxia. We showed that chloroquine suppressed CCA cell migration under hypoxic-mimicking conditions on exposure to $100{\mu}M$ $CoCl_2$. Moreover, chloroquine stabilized the protein level of prolyl hydroxylase domain proteins (PHD-2) but reduced the levels of hypoxic responsive proteins such as hypoxia-inducible factor (HIF-$1{\alpha}$) and vascular endothelial growth factor (VEGF). It also suppressed epithelial mesenchymal transition (EMT) by increasing the ratio of E-cadherin to N-cadherin under hypoxic conditions. In conclusion, chloroquine can inhibit hypoxia-stimulated metastasis via HIF-$1{\alpha}$/VEGF/EMT which may serve as a useful additional strategy for CCA therapy.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.89-92
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• 2001

Aspergillus niger has been used as a host system to express many heterologous proteins. It has various advantages over other expression systems in that it is a small eukaryotic GRAS (Generally Recognized aS Safe) organism with a capacity of secreting large amount of foreign proteins. However, it has been known that the presence of an abundant protease is a limiting factor to express a heterologous protein. The proteases deficient mutants of A. niger were obtained using UV -mutagenesis. A total of 1 ${\times}$ $10^5$ spores were irradiated with 10-20% survival dose of UV, 600J/M2 at 280nm, and the resulting spores were screened on the casein -gelatin plates. Ten putative protease deficient mutants were further analyzed on the starch plates to differentiate the pro from the secretory mutant. An endogenous extracellular enzyme, glucose oxidase, was also examined to confirm that the mutant phenotype was due to the proteases deficiency rather than the mutation in the secretory pathway. The reduced proteolytic activity was measured using SDS-fibrin zymography gel, casein degradation assay, and bio-activity of a supplemented hGM -CSF (human Granulocyte-Macrophage Colony Stimulating Factor). Comparing with the wild type strain, less than 30 % of proteolytic activity was observed in the culture filtrate of the protease deficient mutant (pro -20) without any notable changes in cell growth and secretion.

• 콘크리트학회논문집
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• 제12권2호
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• pp.63-69
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• 2000

This study investigates a new method of using a concrete disk to calculate stress intensity factor (SIF) for mixed mode cases. The results indicate that the disk method is more accurate than three point bending test (TPB) in obtaining correct SIF values for mixed mode fracture propagation. Stress intensity factors $K_{I}$ and $K_{II}$ are calculated using a center notched disk subjected to splitting load. The notch angle is calculated by finite element (FEM). Fracture toughness $K_\textsc{k}$ of the concrete is obtained from the load intensities at the initiation of crack propagation. According to the finite element analysis(FEA) and disk test, the results show that mode I and mixed mode cracks propagate toward the directions of crack face and loading point, respectively. The results from FEA with maximum stress theory compare well with the experimental date. Unlike TPB method where an accurate fracture toughness value is difficult to obtain due to the irregular shape of load deflection curve and delayed final crack propagation (following slow stable cracking). fracture toughness value is easily measured in the disk test from the crack initial load. Therefore, it is safe to conclude that disk method is more advantageous than TPB method in analyzing combined mode fracture problems.

• International Journal of Fluid Machinery and Systems
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• 제8권3호
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• pp.155-168
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• 2015

No-load speed is an important performance factor for the safe operation of hydropower systems. In turbine design, the manufacturers must conduct several model tests to calculate the accurate value of no-load speed for the complete range of operating conditions, which are expensive and time-consuming. The present study presents steady and unsteady methods for calculating no-load speed of a Francis turbine. The steady simulations are implemented using a commercial flow solver and an iterative algorithm that relies on a smooth relation between turbine torque and speed factor. The unsteady method uses unsteady RANS simulations that have been integrated with a user subroutine to compute and return the value of runner speed, time step and friction torque. The main goal of this research is to evaluate and compare the two methods by calculating turbine dynamic parameters for three test cases consisting of high and medium head Francis turbines. Overall, the numerical results agreed well with experimental data. The unsteady method provided more accurate results in the opening angle range from 20 to 26 degrees. Nevertheless, the steady results showed more consistency than unsteady results for the three different test cases at different operating conditions.