• Korean Journal of Adult Nursing
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• v.18 no.5
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• pp.691-698
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• 2006

Purpose: This research was conducted to evaluate, analyze, and determine the limitation of the anesthesia that is used in there covery room in order to provide the foundation for developing the effectiveness of it. Method: There covery records of this study were collected from 41 hospitals in Seoul, Kyung Kii-Do, and Inchon province. The post anesthetic recovery records consisted of the evaluation of type, evaluative items, frequency, time, score and $SaO_2$. These records were collected from September to December of 2005. Results: The most commonly used post anesthetic evaluation tool was Alderete Score, which was used in 73% of hospitals. The second was Aldrete Score with $SaO_2$(17%). Also, 5% of the hospitals used the modified AS form. There were 2.5% of the hospitals applying the adult and child in the evaluation separately. Also, the last 2.5% of the hospitals did not use AS but measured BP, pulse, respiration, temperature, and $SaO_2$ with observing nausea, vomiting, urinary retention, backache, laryngitis, shock, and neurologic assessment. Conclusion: It is necessary to develop a post-anesthetic recovery evaluation tool including the application of $SaO_2$, so that the early-diagnosis of hypoxia can be possible. In addition, it is necessary to develop a post- anesthetic recovery evaluating method that can distinguish a difference between adults and children.

• Journal of the Optical Society of Korea
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• v.19 no.4
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• pp.403-408
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• 2015

To evaluate the effects of spherical aberration (SA) correction on optical quality in pseudophakic eyes, we simulated the optical quality of the human eye by computation of the modulation transfer function (MTF). We reviewed the medical records of patients who underwent cataract surgery in Asan Medical Center, retrospectively. A Zywave aberrometer was used to measure optical aberrations at 1-12 postoperative months in patients with AR40e intraocular lens implants. The MTF was calculated for a 5 mm pupil from measured wavefront aberrations. The area under the MTF curve (aMTF) was analyzed and the maximal aMTF was calculated while changing the SA ($-0.2{\sim}+0.2{\mu}m$) and the defocus (-2.0 ~ +2.0 D). Sixty-four eyes in 51 patients were examined. The maximal aMTF was $6.61{\pm}2.16$ at a defocus of $-0.25{\pm}0.66D$ with innate SA, and $7.64{\pm}2.63$ at a defocus of $0.08{\pm}0.53D$ when the SA was 0 (full correction of SA). With full SA correction, the aMTF increased in 47 eyes (73.4%; Group 1) and decreased in 17 eyes (26.6%; Group 2). There were statistically significant differences in Z(3, -1) (vertical coma; P = 0.01) and Z(4, 4) (tetrafoil; P = 0.04) between the groups. The maximal aMTF was obtained at an SA of $+0.01{\mu}m$ in Group 1 and an SA of $+0.13{\mu}m$ in Group 2. Optical quality can be improved by full correction of SA in most pseudophakic eyes. However, residual SA might provide benefits in eyes with significant radially asymmetric aberrations.

• Bulletin of the Korean Chemical Society
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• v.30 no.5
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• pp.1101-1106
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• 2009

Multivalent and multi-specific antibodies can provide valuable tools for bio-medical research, diagnosis and therapy. In antigen-antibody interactions, the avidity of antibodies depends on the affinity and the number of binding sites.$^1$ As artificial multivalent antibody agents, single chain Fv-streptavidin fusion tetramer proteins $(scFv-SA)_4$ have been previously tested.$^{1,\;2}$ Although, the Fab domain is known to be more stable than scFv in animal models,$^{3,\;4}$ it has never been used to make a multivalent agent with a streptavidin fusion. In this study, we prepared tetra-valent $(Fab-cSA)_4$ by fusing Fab with core streptavidin (cSA). This molecule was made using inclusion body production, refolding and chromatography purification. Affinities of the Fab-cSA tetramer and a scFv-cSA tetramer to a cell surface antigen were compared by ELISA using biotin-HRP. The Fab-cSA tetramer showed higher binding avidity than the scFv-cSA tetramer. The higher binding avidity of the Fab-cSA tetramer demonstrates its potential as a therapeutic agent for target-specific antibody therapy.

• Applied Chemistry for Engineering
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• v.23 no.3
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• pp.320-325
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• 2012

The catalytic cracking of waste lubricant oil was carried out on silica-alumina (SA), hydrogen-type mordenite (HM), and dealuminated mordenite (DM) with the silica/alumina ratio of 10.5, 10, and 12.5, respectively. Activity in the catalytic cracking was found to be in the order of SA > DM > HM. Carbon number distribution of the oil obtained over SA was similar to that of gasoline while that of the oil obtained over DM was similar to that of diesel. Carbon number distribution of the oil obtained over HM was similar to that between gasoline and diesel. Acid amounts of three kinds of catalysts were found to be in the order of $SA\;{\approx}\;HM$ > DM. Unlike HM and DM with pores of an uniform diameter below 10 A, SA had a pore size distribution within the range of 10 to 50 A. These results indicate that the acid amount and pore size of the catalysts may be related to the carbon number distribution of the cracked oil. The decrement of surface area by the accumulation of carbon and impurities on the surface of the catalyst was found to be in the order of SA > DM > HM.

• Food Science and Biotechnology
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• v.17 no.2
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• pp.434-437
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• 2008

The aim of this study was to investigate whether Monascus-fermented soybean extracts (MFSE) containing natural estrogen-like compounds such as isoflavones and mevinolins has potential effects on human osteoblast-like SaOS2 cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and alkaline phophatase (ALP) assaies. MFSE exerted biphasic dose-dependent effect; stimulating osteoblastic activity at low concentrations and inhibiting SaOS2 cells viability at high concentrations. At $10^{-8}-10^{-4}\;mg/mL$, MFSE is not only non-cytotoxic but also induced comparatively high ALP activity on SaOS2 cells. ALP activity (%) significantly increased (220.1%, p<0.05) when SaOS2 cells were treated with MFSE at a concentration of $10^{-5}\;mg/mL$, whereas slowly increased (185.6%, p<0.05) in unfermented soybean extracts (UFSE) at $10^{-3}\;mg/mL$. The potentially greater ALP activity of MFSE compared to the UFSE might partially be caused by its mevinolin, which was derived from the soybean during Monascus-fermentation. Our findings indicate that supplementation of MFSE may accelerate the speed of intracellular ALP synthesis by the bone cells when provided at optimal dosages.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.2
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• pp.167-173
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• 2016

This study investigated the effects of green tea polyphenol and vitamin C on type 2 diabetes mellitus by administering polyphenon 60 (P60) and sodium ascorbate (SA) to diabetic rats induced by high fat diet/low-dose streptozotocin. The experimental group was divided into five different groups: non-diabetic control group (NC), diabetes control group (DC), diabetes+P60 group (DM+P60), diabetes+SA group (DM+SA), and diabetes+P60+SA group (DM+P60+SA). P60 and SA were dissolved in 0.1% drinking water. After P60 and SA were administered for 16 weeks, fasting blood glucose, plasma insulin, serum triglyceride, blood urea nitrogen (BUN), and creatinine levels as well as kidney alkaline phosphatase (AP) and ${\gamma}$-glutamyltranspeptidase (GGT) activities were measured. Fasting blood glucose level increased 5-fold in the DC group compared to the NC group. In the DM+P60 group, fasting blood glucose level decreased by 14%. In the DM+P60+SA group, fasting blood glucose level decreased by 28% compared to the DC group, whereas the DM+SA group did not show any significant difference. The homeostasis model assessment for insulin resistance index increased in the DC group and decreased in the DM+P60+SA group compared to the DC group. Serum creatinine level increased in the DC group, but decreased by 17% in the DM+P60 group and by 43% in the DM+P60+SA group compared to the DC group. The serum BUN level increased in the DC group, but decreased by 41% in the DM+P60+SA group compared to the DC group. Kidney GGT and AP activities decreased in the DC group compared to the NC group; however, they were reversed by DM+P60+SA group. These results show that combined administration of both green tea polyphenol and vitamin C had better effects on improving blood glucose level, insulin resistance, serum triglyceride level, and protecting kidneys than administration of either green tea polyphenol or vitamin C alone in the context of type 2 diabetes.

• Mass Spectrometry Letters
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• v.5 no.2
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• pp.49-51
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• 2014

The effectiveness of tertiary matrices composed of the combination of three common matrices (dihydrobenzoic acid (DHB), ${\alpha}$-cyano-4-hydroxycinnamic acid (CHCA), and sinapinic acid (SA)) was compared with that of single or binary matrices in the analysis of polyethylene glycol (PEG) polymers ranging from 1400 to 10000 Da using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A tertiary matrix of 2,5-DHB+CHCA+SA was the most effective in terms of S/N ratios. CHCA and CHCA+SA produced the highest S/N ratios among the single matrices and the binary matrices, respectively. The improvement observed when using a tertiary matrix in analyses of PEG polymers by MALDI-TOF MS is believed to be due to the uniform morphology of the MALDI sample spots and synergistic effects arising from the mixture of the three matrix materials.

• Journal of Environmental Health Sciences
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• v.26 no.3
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• pp.86-91
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• 2000

The purpose of this was to investigate the degradation efficiency of phenol compounds(catechol, ferulic acid, protocatechuic acid, syringic acid, vanillic acid) by Streptomyces halstedii scabies SAI-36, Streptomyces avendulas SA2-14, and Strptomyces badius(ATCC 39117, control group). The results were as follows: Catechol showed the degradation efficiency that is lower than 50% in three strains. Ferulic acid and vanillic acid showed high degradation efficiency of 98.8% and 94.5% respectively by Streptomyces lavendulas SA2-14. protocatechuic acid and syringicacid showed high degradation efficiency of 89.6% and 77.9%. The degradation efficiency of catechol by Streptomyces halstedii scabies SAI-36, Streptomyces lavendulas SA2-14 and Streptomyces badius(ATCC 39117) was low as 49.2%, 40.2% and 20.2% respectively. But the degradation of other phenolic compoumds except catechol by Streptomyces laven-dulas SA2-36 and Streptomyces badius(ATCC 39117). The results demonstrated that two experimental strains are superior ability to control group in degradation of phenol compounds and Streptomyces lavendulas SA2-14 was superior of two experimental strain. This results were consistent with previous research results that Streptomyces lavendulas SA2-14 was the best strain in degradation ability for lignin, decoloration abilities for variousdyes, and various enzyme production abilities. Therefore, it is suggested that lignin can be used as a indicator when selecting Actinomycetes for degradation of non-degradable materials such as phenol compounds.

• The Journal of the Korean Society for Microbiology
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• v.5 no.1
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• pp.1-8
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• 1970

This report is to observe the rate of multiple resistance to various antibiotics among staphylococcus aureus and E. coli which were isolated with high frequency at our department during 1969. 1. One hundred fifty two strains(20.6%) resistant to four antibiotics multiple resistant was the highest incidence among 739 strains of Staphylococcus aureus. 2. Regarding the multiple antibiotic resistant of Staphylococcus aureus, 75strains(10.1%) to SA. PC. TC the three antibiotics resistant, have been observed, with the highest incidence, and next to this, 69 strains(9.3%) to SA. PC. SM. TC the four autibiotics resistant, and 68 strains(9.2%) to SA. PC. SM. CM. TC. SM. the six antibiotics resistant, have been observed in descending order. 3. The case of one hundred twenty seven strains(28.0%) of the six antibiotic multiple resistant among 454 strains of Escherchia coli was the highest incidence. 4. Concerning the multiple antibiotic resistant of Escherichia coli, 70 strains(l5.4%) to SA. SM. CM. TC. EM. FD. the six antibiotics resistant, have been observed, with the highest incidence, and next to this, 59 strains(l3.0%) to SA. SM. CM. TC. EM. FD. AC the seven antibiotics resistant, and 42 strains(9.3%) to SA. SM. CM. TC. EM. FD. KM. AC the eight antibiotics resistant have been observed in descending order.

• Applied Chemistry for Engineering
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• v.25 no.2
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• pp.227-232
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• 2014

Depolymerization of sodium alginate (SA) was carried out by electron beam irradiation in a hydrogen peroxide atmosphere. E-beam with 1.0 and 2.5 MeV of accelerating voltages were employed in this experiment. For control of molecular weight and the radiation yield of scission ($G_s$), the irradiation dosage of e-beam was managed in a range from 2.5 to 20 kGy while the quantity of hydrogen peroxide was adjusted in a range of 0 to 4.5%. The chemical structure of the depolymerized sodium alginate (DSA) was analyzed to have scission of 1,4-glycoside bond mainly and a few fragmentary formate end groups which may be produced by the cleavage between C2 and C3 in repeating unit of alginate. It turned out to have simple chemical structures at the DSA end groups, produced by e-beam irradiation, similar with those in the polymer SA structure. As a result, the molecular weight of SA decreased as the energy and dosage of applied e-beam increased, and the radiation yield of scission showed the best result at 2w/v% in SA concentration. The highest radiation yield of scission ($7.919{\times}10^4mol/J$) was confirmed when an irradiation dosage of 20 kGy (2 MeV) and 1.5% hydrogen peroxide were used in 2% SA aqueous solution.