• The Korea Journal of Herbology
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• v.33 no.4
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• pp.19-26
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• 2018

Objectives : The aim of this study was to investigate the immunopotentiating activity of combine extract that Silkworm and Cinnamomum cassia. Recently, acute epidemic diseases such as cold and viral respiratory diseases have been emerging. So, interested in immunity enhancement has been increasing, and research on natural products to promote immunity activity has been actively conducted. Methods : To confirm the immunopotentiating activity effect, Silkworm (SW), Cinnamomum cassia (CC), and SWCC combined extracts were treated 14 days at 300 mg/kg/day. The changes of glutamic oxalacetic transaminase (GOT), glutamic pyruvate transaminase (GPT) in serum were analyzed after experiment. The changes in the total spleen cell number were measured. Immune cells in spleen were analyzed using fluorescence activated cell sorter (FACS). also, analyzed the expression of cytokines in spleen. Results : Total number of cells in the spleen and FACS analysis of T lymphocytes activated in the spleen showed that the SWCC combined treated group had much higher frequency of active cells than both single groups. The ratio of CD4+CD8+, CD4+CD69+ and CD4+CD25+ T cells in spleen, SWCC is higher than other groups except Nor in CD4+, CD4+CD69+, CD4+CD25+ T cells. The results of this study suggest that SWCC can help immune function via IL-2, IL-10, IL-12, IFN-${\gamma}$ cytokine production, increased T lymphocytes and splenocyte proliferation. Conclusion : Therefore, these results suggested that the SWCC combined extracts administration increase stronger immunity enhancement than when SW and CC adminstration.

• KIPS Transactions on Computer and Communication Systems
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• v.11 no.6
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• pp.193-204
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• 2022

In a smart factory environment in a small and medium-sized enterprise (SME) environment, sensors and actuators operating on actual manufacturing lines, programmable logic controllers (PLCs) to manage them, human-machine interface (HMI) to control and manage such PLCs, and consists of operational technology server to manage PLCs and HMI again. PLC and HMI, which are in charge of control automation, perform direct connection with OT servers, application systems for factory operation, robots for on-site automation, and production facilities, so the development of security technology in a smart factory environment is demanded. However, smart factories in the SME environment are often composed of systems that used to operate in closed environments in the past, so there exist a vulnerable part to security in the current environment where they operate in conjunction with the outside through the Internet. In order to achieve the internalization of smart factory security in this SME environment, it is necessary to establish a process according to the IEC 62443-4-1 Secure Product Development Life cycle at the stage of smart factory SW and HW development. In addition, it is necessary to introduce a suitable development methodology that considers IEC 62443-4-2 Component security requirements and IEC 62443-3 System security requirements. Therefore, this paper proposes an application plan for the IEC 62443 based development security process to provide security internalization to smart factories in an SME environment.

• Journal of Life Science
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• v.34 no.4
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• pp.236-244
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• 2024

Melanin is a natural pigment found in most plants and animals, and it is involved in determining the color of the skin and hair. Melanogenesis is a reactive occurrence in melanocytes aiming to protect the skin from external stimuli, such as ultraviolet rays. Tyrosine is involved in the biosynthesis of the substrate tyrosine into melanin. However, melanin overproduction can lead to skin diseases, such as melasma, blotching, hyperpigmentation, and skin cancer. Although many studies have been conducted on whitening substances, such as kojic acid and arbutin, some countries have banned or refrained from using them due to their side effects. Therefore, this study assessed the potential of horseradish (HR) as a new whitening agent in cosmetic products. For efficient extraction, subcritical water extraction was conducted. The results showed that the horseradish subcritical water 200℃ (SW 200) extract showed high DPPH radical scavenging ability, total phenolic contents (TPC), inhibiting tyrosinase activity and inhibiting melanin production of B16-F10 melanoma cell lines. To investigate its cytotoxicity to the B16-F10 melanoma cell lines, MTT reduction assay and morphological changes were observed. No cytotoxicity was found in horseradish methanol extract and SW 200. In conclusion, this research suggests the possibility of horseradish subcritical water may be useful as a natural whitening ingredient to be used in cosmetic products.

• Journal of Applied Biological Chemistry
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• v.59 no.1
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• pp.31-36
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• 2016

Chlorine dioxide has been used for a disinfectant by exhibiting antimicrobial activity and is also potent to kill insect pests infesting stored grains. This study aimed to extend the usefulness of chlorine dioxide with respect to anticancer and antiviral activities. Cytotoxicity of chlorine dioxide was assessed against five different human cancer cell lines. Chlorine dioxide exhibited significant cytotoxicity against two breast cancer cell lines (MCF-7, MDA-MB-231) and three colorectal cancer cell lines (LoVo, HCT-116, SW-480). This cytotoxicity appeared to be associated with the capacity of chlorine dioxide to induce the production of reactive oxygen species (ROS). Compared to control insect cell lines, the cancer cell lines possessed much higher levels of ROS. On the other hand, a treatment of an antioxidant, vitamin E, significantly reduced the cytotoxicity, suggesting that the cytotoxicity was induced by high levels of ROS production. Chlorine dioxide exhibited antiviral activity against different viruses. A baculovirus, Autographa californica nuclear polyhedrosis virus (AcNPV), is a dsDNA insect virus and lost its viral activity to form polyhedral viral particles in response to chlorine dioxide. The antiviral activity against AcNPV was dependent on the incubation time with chlorine dioxide. Tobacco mosaic virus is a ssRNA plant virus and was reduced in its population after exposure to chlorine dioxide along with significant decrease of viral symptoms. These results indicate that chlorine dioxide possesses anticancer and antiviral activities probably due to its inducing activity of ROS production.

• Journal of Digital Convergence
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• v.11 no.8
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• pp.191-197
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• 2013

In this paper, we implemented a paper currency recognition system using IR(infrared) sensor. The system has 32 channel IR sensor to measure the reflection and penetration quantity of light. The IR image of paper currency of 10-bit gray scale is used to differentiate the real and counterfeit paper currency with image information from 0 to 4095. The characteristics of IR image are recognized by brightness and darkness and the positions of bright and dark portions are different between real and counterfeit paper currency. The price of IR sensors were relatively high, however, it is good price in these days due to mass production to apply to counterfeit detection area. We used a software table having the IR characteristics of real paper currency to compare with the IR images of the input paper currency. The performance of the implemented system shows 1-2% error rates for Euro real paper currency and 0% error rates for various counterfeit paper currencies of several countries.

• Journal of The Korean Association of Information Education
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• v.18 no.1
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• pp.173-184
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• 2014

The ability to need information-oriented society is the convergence ability based in information ability. A new curriculum is demanded by new technology and circumstance to need the core ability. Korea Association of Information Education have studied a new curriculum and suggest a new plan that content of information education classified by 'computer system', 'SW production' and 'convergence activities'. Therefore, we studied on convergence activities of K-9. In this paper we firstly suggested concept of convergence ability as activities to use ICT, information communication ethics and robot. We nextly suggested object to achieve, teaching-learning methods and evaluation methods of convergence activities. We expect the curriculum we suggested about convergence activities will contribute to training talented individuals.

• Journal of The Korean Association of Information Education
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• v.18 no.2
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• pp.235-242
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• 2014

Many famous institutes or associations select ICT ability by major ability to need in 21C's information-oriented society. Korea Association of Information Education have studied a new curriculum and suggest a new plan that content of information education classify by 'computer system', 'SW production' and 'fusion activity'. Therefore we study on productivity tools of elementary and middle school. In this paper we firstly suggest object to achieve on productivity tools of elementary and middle school. Next we suggest teaching learning method and evaluation method on productivity tools. we expect that the object to achieve on productivity tools we suggest will contribute to develop the curriculum of ICT subject.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.7
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• pp.931-937
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• 2006

The full-length cDNA of the porcine SMPX gene was obtained by the rapid amplification of cDNA ends (RACE). The nucleotide sequences and the predicted protein sequences share high sequence identity with both human and mouse. The promoter of SMPX was sequenced and then analyzed to find the promoter binding sites. The reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that SMPX has a high level of expression in heart and skeletal muscle, a very low expression in lung and spleen and no expression in liver, kidney, fat and brain. Moreover, SMPX has a differential expression level in skeletal muscle, the expression in 65-day embryos being higher than other stages. The porcine SMPX was mapped to SSCXp24 by using a somatic cell hybrid panel (SCHP) and was found closely linked to SW1903 using the radiation hybrid panel IMpRH. An A/G single nucleotide polymorphism (PCR-RFLP) in the 3'-untranslated region (3'-UTR) was detected in eight breeds. The analysis of allele frequency distribution showed that introduced pig breeds (Duroc and Large White) have a higher frequency of allele A while in the Chinese indigenous pig breeds (Qingping pig, Lantang pig, YushanBlack pig, Large Black-White pig, Small Meishan) have a higher frequencies of allele G. The association analysis using an experimental population (188 pigs), which included two cross-bred groups and three pure-blood groups, suggested that the SNP genotype was associated with intramuscular fat content.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.12
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• pp.1675-1681
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• 2016

The aim of this study was to detect positional candidate genes located within the support interval (SI) regions based on the results of red blood cell, mean corpuscular volume (MCV), and mean corpuscular hemoglobin quantitative trait locus (QTL) in Sus scrofa chromosome 13, and to verify the correlation between specific single-nucleotide polymorphisms (SNPs) located in the exonic region of the positional candidate gene and the three genetic traits. The flanking markers of the three QTL SI regions are SW38 and S0215. Within the QTL SI regions, 44 genes were located, and runt-related transcription factor 1, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A (DYRK1A), and potassium inwardly-rectifying channel, subfamily J, member 15 KCNJ15-which are reported to be related to the hematological traits and clinical features of Down syndrome-were selected as positional candidate genes. The ten SNPs located in the exonic region of the three genes were detected by next generation sequencing. A total of 1,232 pigs of an $F_2$ resource population between Landrace and Korean native pigs were genotyped. To investigate the effects of the three genes on each genotype, a mixed-effect model which is the considering family structure model was used to evaluate the associations between the SNPs and three genetic traits in the $F_2$ intercross population. Among them, the MCV level was highly significant (nominal $p=9.8{\times}10^{-9}$) in association with the DYRK1A-SNP1 (c.2989 G$F_2$ intercross, our approach has limited power to distinguish one particular positional candidate gene from a QTL region.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.17 no.7
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• pp.1740-1746
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• 2013

Augmentative and alternative communication(AAC) is a communication tool for those with impairments or restrictions on the production or comprehension of spoken language. Traditional AAC has used a portable board with pictures and texts with which disabled person could be supported by it. Currently, smart phone based AAC researches and developments are actively studying. With emerging concepts of context-aware computing, the mobile devices can provide mobile users with timely information and location based services. We proposed a adjustable AAC system for disabled people of which has variable layouts and selectable items. We also implemented context-ware application for AAC systems including time, location and click count informations on smart phone. The system can adaptively change its layout and select AAC items depending on various situation of disabled persons.