• Reproductive and Developmental Biology
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• 제36권1호
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• pp.21-26
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• 2012

Suspension culture is a useful tool for culturing embryonic stem (ES) cells in large-scale, but the stability of pluripotency and karyotype has to be maintained $in$ $vitro$ for clinical application. Therefore, we investigated whether the chromosomal abnormality of ES cells was induced in suspension culture or not. The ES cells were cultured in suspension as a form of aggregate with or without mouse embryonic fibroblasts (MEFs), and 0 or 1,000 U/ml leukemia inhibitory factor (LIF) was treated to suspended ES cells. After culturing ES cells in suspension, their karyotype, DNA content, and properties of pluripotency and differentiation were evaluated. As a result, the formation of tetraploid ES cell population was significantly increased in suspension culture in which ES cells were co-cultured with both MEFs and LIF. Tetraploid ES cell population was also generated when ES cells were cultured alone in suspension regardless of the existence of LIF. On the other hand, the formation of tetraploid ES cell population was not detected in LIF-free condition, in which MEFs were included. The origin of tetraploid ES cell population was turned out to be E14 ES cells and not MEFs by microsatellite analysis and the basic properties of them were still maintained despite ploidy-conversion to tetraploidy. Furthermore, we identified the ploidy shift from tetraploidy to near-triploidy as tetraploid ES cells were differentiated spontaneously. From these results, we demonstrated that suspension culture system could induce ploidy-conversion generating tetraploid ES cell population. Moreover, optimization of suspension culture system may make possible mass-production of ES cells.

• Journal of Plant Biology
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• 제12권4호
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• pp.9-18
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• 1969

In the previous paper(part III), a certian relationships between the changes of chlorinity and organic acid released from organic material were seemed to be concened to each other in saline soil suspension. Such a possibility had been a cause to take this experiments and this experiment was carried out under the treatment of organic acid crystal, oxalic acid and succinic acid, to the soil suspension(soil: water=20g:40cc) directly. The amount of organic acid treated to the suspension were related to the contents of organic material, as amount of organic acid per gram of organic material(391.76mg). The saline soil suspension were grouped and treated with the acids in order of 78.35mg(Group 1), 391.76mg(Group 2) 979.4mg(Group 3), and 1958.8mg(Group 4), respectively. Treated suspension had been incubated at room temperature and extract from suspension was used for analysis. Followings are summary of this report. 1) Changes of pH in soil suspension appeared a little increase after the treatment of organic acid until 168 hours. 2) Total acidity of soil suspension showed a variation, however, the values of total acidity appeared not to be increased or decreased during the period of experiment. 3) Sugar contents of soil suspension was increased until 168 hours after treatment. These results are similar tendency to the previous paper. 4) Addition of organic acid to soil suspension was confirmed not to be effective method for desalination from saline soil. Chlorinity of group 3 and 4 which were treated with high concentration of organic acid showed a decrease comparing to control group.

• 한방재활의학과학회지
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• 제19권4호
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• pp.19-35
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• 2009

Objectives : This experimental study was designed to investigate the effect of KangwhalSokdan-tang(Jianghuoxuduan-tang) on the muscle regeneration of atrophied rat muscle by hindlimb suspension. Materials and methods : In this study, Sprague-Dawley rats weighing about 250g were subjected to hindlimb suspension and divided into total four groups: Normal group(n=6), Control group(n=6), Hindlimb non-treatment group(n=6), Hindlimb treatment group(n=6). Experiments were seperately tried two times. The first trial was studied by the following two groups; The first was normal group(n=6). The second was group(n=18) for hindlimb suspension during 2 weeks (control I group). The second trial after 2 weeks hindlimb suspension was studied by the following three groups; The third group(n=6) was expired immediately after 2 weeks hindlimb suspension. The forth group(n=6) was given free activity during 2 weeks after 2 weeks hindlimb suspension. The fifth group(n=6) was administrated of KST during 2 weeks after 2 weeks hindlimb suspension. In order to investigate degree of muscle atrophy, body weight and gastrocnemius muscle mass were compared. To analyze muscle regeneration factors(expression of IGF-1, Myogenin, MyoD), Western blot was used. Results : The results were analyzed by statistical process as follows, 1. In body weight, all hindlimb suspension groups were lower than normal group, but tendency of increase was shown in KST group compared to non-treatment group after 2 weeks hindlimb suspension. 2. In gastrocnemius muscle mass, KST group on both side was significantly higher than non-treatment group after 2 weeks hindlimb suspension. 3. In case of IGF-I, Type I of KST group was significantly increased than non-treatment group, but Type II was not shown significance. 4. There was no significantly difference in Myogenin. 5. In MyoD, Type I of KST group was significantly increased than control group, and Type II of KST group was significantly increased than non-treatment group. Conclusions : In summary, this study demonstrates that KST administration has an effect to prevent muscle atrophy and contribute muscle regeneration and proliferation. And also it is suggested that IGF-I and MyoD is major factors of myogenesis expression to KST adminstration after hindlimb suspension.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2009년도 추계학술대회 논문집
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• pp.519-523
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• 2009

This paper presents the analysis of pitch static attitude (PSA) in thermally assisted magnetic recording (TAMR) suspension. The TAMR suspension using an optical fiber has high stiffness such as vertical, pitch and roll. Therefore, P-torque is greatly increased by the optical fiber. Also, flying height (FH) of the slider with TAMR suspension can be largely changed by PSA. In this paper, we focus on the FH by PSA of TAMR suspension. The FH is investigated using various PSA and proper PSA is proposed.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1996년도 Proceedings of the Korea Automatic Control Conference, 11th (KACC); Pohang, Korea; 24-26 Oct. 1996
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• pp.393-396
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• 1996

This paper studies electrostatically suspended induction motors (ESIM). The ESIM possesses the rotating ability of an ordinary electrostatic induction motor, in addition to providing contactless support by electrostatic suspension. To accomplish these two functions, a feedback control strategy and the operating principle of an ordinary electrostatic induction motor are used. The stator possesses electrodes which exert the electrostatic forces to the rotor and are divided into a part responsible for suspension and one for rotation. Two rotor types are utilized: a polished glass disk without any surface treatment, and a polished glass disk covered with a thin layer of conductive material (ITO layer) on only one side. In this paper, the principle of the ESIM is described, followed by stator electrode design, experimental apparatus, control strategy for stable suspension. Experimental results show that the glass disk has been rotated with a speed of approximately 70 rpm while being suspended stably at a gap length of 0.3 mm.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.278-281
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• 2003

The focus of this research is to realize the function which is equivalent to the active suspension system, with controlling semi-active suspension through the attenuation of power variable damper in lower cost and smaller energy. Actually some semi-active suspension systems have been adopted, but they are not sufficient in performance. The authors intended to develop more effective and practical system and applied the optimal control technique. The results of experiments with practical suspension system showed a degree of improvement of comfortableness.

• 한국자동차공학회논문집
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• 제6권3호
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• pp.154-160
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• 1998

Ride quality of medium truck became a very important factor in the suspension design, to the demand of more comfortable ride of passengers. This study describes how to determine and evaluate design parameters related to the chassis suspension system with time and frequency analysis. The spring stiffness and damping force of the chassis suspension system were obtained by observing the vertical acceleration PSD. The simulation was carried out on various road profiles, which was suggested by ISO. The pitching motion of the medium size truck was observed to improve the ride quality. A computer simulated truck model was constructed using DADS, a commercial dynamic analysis software, in order to simulate the truck motions. From the analyzed process of suspension parameters, it was concluded that the spring and the shock absorbers affect the pitching of the vehicle. In order to validate the computer simulated truck model, a physical prototype was constructed and tested.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 추계학술대회
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• pp.619-624
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• 2004

This paper applies the fuzzy logic controller to a semiactive seat suspension system in order to obtain the better ride comfort in constraint of specific rattle space. The seat suspension system used for this research is a scissors-type one with the MR (Magnetic Rheological) fluid damper. Since a seat suspension system with a driver can not be exactly modeled, it is effective to control with the fuzzy logic controller. The rule was carefully tuned to effectively reduce the vibration transmitted to a driver. The on-road ride was realized on a hydraulic excitor and the result shows that the fuzzy controller has reduced the vibration of a seat suspension system compared to the continuous skyhook controller.

• 한국정밀공학회지
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• 제20권7호
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• pp.201-207
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• 2003

Sensitivity analysis of suspension elements of an articulated bogie for light railway vehicles is presented. The ride, stability and safety are used as dynamic performance indices. Suspension elements of 10 and a conicity of wheel are used as design variables. To analyze sensitivity of design variables. the railway vehicle dynamics analysis program AGEM is used. The results show that the secondary suspension elements have a strong effect on ride and the primary suspension elements have a moderate effect on ride. Conicity of wheel has a strong effect on the stability. The safety is not effected by all the design variables.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2007년도 춘계학술대회논문집
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• pp.1125-1130
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• 2007

This paper presents a maneuver analysis of a full-vehicle featuring electrorheological (ER) suspension and ER brake. In order to achieve this goal, an ER damper and an ER valve pressure modulator are devised to construct ER suspension and ER brake systems, respectively. After formulating the governing equations of the ER damper and ER valve pressure modulator, they are designed and manufactured for a middle-sized passenger vehicle, and their field-dependent characteristics are experimentally evaluated. The governing equation of motion for the full-vehicle is then established and integrated with the governing equations of the ER suspension and ER brake. Subsequently, a sky-hook controller for the ER suspension and a sliding mode controller for the ER brake are formulated and implemented. Control performances such as vertical displacement and braking distance of vehicle are evaluated under various driving conditions through computer simulations.