• Special Issue of the Society of Naval Architects of Korea
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• 2008.09a
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• pp.90-95
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• 2008

The letters and marks on the hull of vessels are marked by welding bead or steel plate to resist the corrosion environment. It has done by manual work. So, it cause deterioration of welding quality and process delay and so on. The automated welding device for draft mark has developed partially in the field of shipbuilding. But it can be used for draft mark only. And it has caused a few problems about that workablity and movablity are decreased owing to the size and weight of device. So we developed the automated welding device that can be used for most letters and marks on the hull. It designed to 3 axises mobile robot include to ratoation axis and stand alone type controller with multi GUI base on imbedded windows.

• Special Issue of the Society of Naval Architects of Korea
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• 2008.09a
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• pp.106-111
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• 2008

A hull form is 181K DWT Bulk Carrier, of which new design and hull form have been developed using CFD tools and model tests. The basic concept design of hull form has been carried out with considering the factors, which are a lot of influence of the wave and viscosity resistance. The considered factors of particular are LCB, DLWL shape, tern and stem profile, Cp-curve shape, etc. Numerical calculations are carried out in the initial design stage and experimental model tests are also carried out in towing tank of MOERI. The variation of the significantly effective characteristics is carried out to achieve optimized hull form. The results from numerical calculations and model test as well as the design procedures to obtain an optimized hull form resent in this paper.

• Special Issue of the Society of Naval Architects of Korea
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• 2008.09a
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• pp.136-143
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• 2008

This paper presents the application of a robot which aims at grinding automatically welding-bead remained in the removal job of working pieces for shipbuilding. In specific, the investigation on this application is composed of two parts; one topic is on the development of a robot platform vertically movable on a steel plate of hull, while the other topic is of the development of a grinding tool mechanism in order to remove welding-beads by using a diamond wheel installed on a servo cylinder (which can result in high working pressure on the grinding wheel). Besides, the development of a vision system for tracking welding-beads as well as recognizing welding surfaces is added for the convenience of this robot application to the removal of welding-beads remained in the working pieces for shipbuilding.

• Special Issue of the Society of Naval Architects of Korea
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• 2011.09a
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• pp.112-116
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• 2011

The noise analysis is usually carried out in the early structure design stage for the main areas in a vessel such as an accommodation, an engine room, HVAC System and etc. If the analysis results are higher than the noise limits based on guideline, appropriate countermeasures are established to reduce noise levels and applied to the vessel. But excessive noise induced the main or auxiliary equipment and high pressure steam system is very difficult to check in the initial design stage, and local noise problems frequently appear in actual vessels. This paper deals with excessive noise of the engine control room on LNG carrier. It includes the cause analysis of excessive noise, the countermeasure, and verification. Also, it proves suitability of the countermeasure through the on-board test.

• Special Issue of the Society of Naval Architects of Korea
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• 2011.09a
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• pp.127-132
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• 2011

In this paper, hydrodynamic performance of FSRU which is designed to operate in North America East Coast assessed. In order to estimate the dynamic performance, the numerical analysis is carried out based on a time domain simulation program to solve the coupled dynamics for floater and mooring lines which is as well known program as DNV SESAM package. The target operating area is East coast of North America and the model test was carried out based on the meta-ocean data of the area. The mooring analysis is only considered wave without other environment condition at this time. The results of the numerical analysis show the under-estimated results at the higher wave height condition. But the tendency is very similar. Also, the motion response show good agreement compared with model test.

• Journal of Microbiology and Biotechnology
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• v.26 no.2
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• pp.432-439
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• 2016

Shiga toxins (Stxs) produced by Shiga toxin-producing Escherichia coli (STEC) strains are major virulence factors that cause fatal systemic complications, such as hemolytic uremic syndrome and disruption of the central nervous system. Although numerous studies report proinflammatory responses to Stx type 1 (Stx1) or Stx type 2 (Stx2) both in vivo and in vitro, none have examined dynamic immune regulation involving cytokines and/or unknown inflammatory mediators during intoxication. Here, we showed that enzymatically active Stxs trigger the dissociation of lysyl-tRNA synthetase (KRS) from the multi-aminoacyl-tRNA synthetase complex in human macrophage-like differentiated THP-1 cells and its subsequent secretion. The secreted KRS acted to increase the production of proinflammatory cytokines and chemokines. Thus, KRS may be one of the key factors that mediate transduction of inflammatory signals in the STEC-infected host.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.3
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• pp.571-575
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• 2004

Escherichia coli O157:H7 cause hemorrhagic colitis and the extraintestinal complication of hemolytic-uremic syndrome, with their higher incidence occurring in children. Lipopolysaccharide (LPS) of E. coli O157:H7 is very important to make IgG anti-LPS with bactericidal activity. To identify the characteristic of E. coli OI57:H7, we isolated 60 MDa plasmid and amplified stx genes of shiga-like toxin (Stx) 1, 2 of E. coli O157:H7 by polymerase chain reaction (PCR) method. Using the simple purification method which contained phenol extract, ethanol precipitation and gel filtration steps, the LPS of E. coli O157:H7 was isolated and purified. Finally, we confirmed the purity of LPS through SDS-PAGE and silver nitrate staining.

• Fisheries and Aquatic Sciences
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• v.16 no.3
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• pp.159-164
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• 2013

The mouse bioassay and high performance liquid chromatography (HPLC) post-column oxidation method are different methods of quantifying paralytic shellfish poisoning toxins. In this study, we compared their ability to accurately quantify the toxicity levels in two types of field sample (oysters and mussels) with different toxin profiles for routine regulatory monitoring. A total of 72 samples were analyzed by both methods, 44 of which gave negative results, with readings under the limit of detection of the mouse bioassay ($40{\mu}g/100g$ saxitoxin [STX] eq). In 14 oysters, the major toxin components were gonyautoxin (GTX) 1, -2, -3, -4, -5, decarbamoylgonyautoxin-2 (dcGTX2), and decarbamoylsaxitoxin (dcSTX), while 14 mussels tested positive for dcSTX, GTX2, -3, -4, -5, dcGTX2, neosaxitoxin (NEO), STX, and dcSTX. When the results obtained by both methods were compared in two matrices, a better correlation ($r^2=0.9478$) was obtained for mussels than for oysters ($r^2=0.8244$). Additional studies are therefore needed in oysters to investigate the differences in the results obtained by both methods. Importantly, some samples with toxin levels around the legal limit gave inconsistent results using HPLC-based techniques, which could have a strong economic impact due to enforced harvest area closure. It should therefore be determined if all paralytic shellfish poisoning toxins can be quantified accurately by HPLC, and if the uncertainties of the method lead to doubts regarding regulatory limits.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2004.05a
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• pp.36-41
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• 2004

STX Shipbuilding Company is under construction design the marine training ship if Korea Maritime University. Processing q this training ship development, model test results and design if general arrangement results are introduced in this paper.

• Special Issue of the Society of Naval Architects of Korea
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• 2006.09a
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• pp.40-45
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• 2006

For ships of ice class, finish Maritime Administration(FMA) requires brackets on intersections between longitudinal frames and the web frames within the ice-strengthened area. The main object of this paper is to verify ultimate load carrying capacity of longitudinal frame without brackets of engine room region of 74,100 DWT Product Oil Tanker. Comparative approach between proposed structures from builder (the proposed structure) and structures satisfying the Finnish-Swedish ice class rules (the rule structure) is used for the analysis.