• 대한조선학회 특별논문집
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• 대한조선학회 2008년도 특별논문집
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• pp.90-95
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• 2008

The letters and marks on the hull of vessels are marked by welding bead or steel plate to resist the corrosion environment. It has done by manual work. So, it cause deterioration of welding quality and process delay and so on. The automated welding device for draft mark has developed partially in the field of shipbuilding. But it can be used for draft mark only. And it has caused a few problems about that workablity and movablity are decreased owing to the size and weight of device. So we developed the automated welding device that can be used for most letters and marks on the hull. It designed to 3 axises mobile robot include to ratoation axis and stand alone type controller with multi GUI base on imbedded windows.

• 대한조선학회 특별논문집
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• 대한조선학회 2008년도 특별논문집
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• pp.106-111
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• 2008

A hull form is 181K DWT Bulk Carrier, of which new design and hull form have been developed using CFD tools and model tests. The basic concept design of hull form has been carried out with considering the factors, which are a lot of influence of the wave and viscosity resistance. The considered factors of particular are LCB, DLWL shape, tern and stem profile, Cp-curve shape, etc. Numerical calculations are carried out in the initial design stage and experimental model tests are also carried out in towing tank of MOERI. The variation of the significantly effective characteristics is carried out to achieve optimized hull form. The results from numerical calculations and model test as well as the design procedures to obtain an optimized hull form resent in this paper.

• 대한조선학회 특별논문집
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• 대한조선학회 2008년도 특별논문집
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• pp.136-143
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• 2008

This paper presents the application of a robot which aims at grinding automatically welding-bead remained in the removal job of working pieces for shipbuilding. In specific, the investigation on this application is composed of two parts; one topic is on the development of a robot platform vertically movable on a steel plate of hull, while the other topic is of the development of a grinding tool mechanism in order to remove welding-beads by using a diamond wheel installed on a servo cylinder (which can result in high working pressure on the grinding wheel). Besides, the development of a vision system for tracking welding-beads as well as recognizing welding surfaces is added for the convenience of this robot application to the removal of welding-beads remained in the working pieces for shipbuilding.

• 대한조선학회 특별논문집
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• 대한조선학회 2011년도 특별논문집
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• pp.112-116
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• 2011

The noise analysis is usually carried out in the early structure design stage for the main areas in a vessel such as an accommodation, an engine room, HVAC System and etc. If the analysis results are higher than the noise limits based on guideline, appropriate countermeasures are established to reduce noise levels and applied to the vessel. But excessive noise induced the main or auxiliary equipment and high pressure steam system is very difficult to check in the initial design stage, and local noise problems frequently appear in actual vessels. This paper deals with excessive noise of the engine control room on LNG carrier. It includes the cause analysis of excessive noise, the countermeasure, and verification. Also, it proves suitability of the countermeasure through the on-board test.

• 대한조선학회 특별논문집
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• 대한조선학회 2011년도 특별논문집
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• pp.127-132
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• 2011

In this paper, hydrodynamic performance of FSRU which is designed to operate in North America East Coast assessed. In order to estimate the dynamic performance, the numerical analysis is carried out based on a time domain simulation program to solve the coupled dynamics for floater and mooring lines which is as well known program as DNV SESAM package. The target operating area is East coast of North America and the model test was carried out based on the meta-ocean data of the area. The mooring analysis is only considered wave without other environment condition at this time. The results of the numerical analysis show the under-estimated results at the higher wave height condition. But the tendency is very similar. Also, the motion response show good agreement compared with model test.

• Journal of Microbiology and Biotechnology
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• 제26권2호
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• pp.432-439
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• 2016

Shiga toxins (Stxs) produced by Shiga toxin-producing Escherichia coli (STEC) strains are major virulence factors that cause fatal systemic complications, such as hemolytic uremic syndrome and disruption of the central nervous system. Although numerous studies report proinflammatory responses to Stx type 1 (Stx1) or Stx type 2 (Stx2) both in vivo and in vitro, none have examined dynamic immune regulation involving cytokines and/or unknown inflammatory mediators during intoxication. Here, we showed that enzymatically active Stxs trigger the dissociation of lysyl-tRNA synthetase (KRS) from the multi-aminoacyl-tRNA synthetase complex in human macrophage-like differentiated THP-1 cells and its subsequent secretion. The secreted KRS acted to increase the production of proinflammatory cytokines and chemokines. Thus, KRS may be one of the key factors that mediate transduction of inflammatory signals in the STEC-infected host.

• 한국식품영양과학회지
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• 제33권3호
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• pp.571-575
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• 2004

대장균 O157:H7의 백신 생산을 위해서 purity가 높은 lipopolysacharride 분리, 정제를 위한 실험을 실시하였다. 대장균 O157:H7 균주를 확인하기 위해서 shiga toxin을 생산할 수 있는 60 MDa plasmid를 분리하였고, PCR법에 의해 E. coli O157:H7 shiga-like toxin(Stx) 1, 2의 stx gene을 증폭하여 E. coli O157:H7의 특징 (130 bp, 346 bp)을 확인하였다. E. coli O157:H7 LPS의 분리 정제는 페놀추출, 에탄올 분획 및 gel filtration의 간단한 방법을 사용하였다. 마지막으로 SDS-PAGE와 silver nitrate 염색을 이용하여 LPS의 purity를 확인하였다.

• Fisheries and Aquatic Sciences
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• 제16권3호
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• pp.159-164
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• 2013

The mouse bioassay and high performance liquid chromatography (HPLC) post-column oxidation method are different methods of quantifying paralytic shellfish poisoning toxins. In this study, we compared their ability to accurately quantify the toxicity levels in two types of field sample (oysters and mussels) with different toxin profiles for routine regulatory monitoring. A total of 72 samples were analyzed by both methods, 44 of which gave negative results, with readings under the limit of detection of the mouse bioassay ($40{\mu}g/100g$ saxitoxin [STX] eq). In 14 oysters, the major toxin components were gonyautoxin (GTX) 1, -2, -3, -4, -5, decarbamoylgonyautoxin-2 (dcGTX2), and decarbamoylsaxitoxin (dcSTX), while 14 mussels tested positive for dcSTX, GTX2, -3, -4, -5, dcGTX2, neosaxitoxin (NEO), STX, and dcSTX. When the results obtained by both methods were compared in two matrices, a better correlation ($r^2=0.9478$) was obtained for mussels than for oysters ($r^2=0.8244$). Additional studies are therefore needed in oysters to investigate the differences in the results obtained by both methods. Importantly, some samples with toxin levels around the legal limit gave inconsistent results using HPLC-based techniques, which could have a strong economic impact due to enforced harvest area closure. It should therefore be determined if all paralytic shellfish poisoning toxins can be quantified accurately by HPLC, and if the uncertainties of the method lead to doubts regarding regulatory limits.

• 한국해양공학회:학술대회논문집
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• 한국해양공학회 2004년도 학술대회지
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• pp.36-41
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• 2004

STX Shipbuilding Company is under construction design the marine training ship if Korea Maritime University. Processing q this training ship development, model test results and design if general arrangement results are introduced in this paper.

• 대한조선학회 특별논문집
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• 대한조선학회 2006년도 특별논문집
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• pp.40-45
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• 2006

For ships of ice class, finish Maritime Administration(FMA) requires brackets on intersections between longitudinal frames and the web frames within the ice-strengthened area. The main object of this paper is to verify ultimate load carrying capacity of longitudinal frame without brackets of engine room region of 74,100 DWT Product Oil Tanker. Comparative approach between proposed structures from builder (the proposed structure) and structures satisfying the Finnish-Swedish ice class rules (the rule structure) is used for the analysis.