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Polychlorobiphenyl (PCB) 토양오염복원: PCB 제거 토양미생물들의 군집과 기능을 효과적으로 분석하는 신 genomics 방법개발에 관한 연구

  • Park Jun-Hong
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2005.04a
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    • pp.28-30
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    • 2005
  • Because of high population diversity in soil microbial communities, it is difficult to accurately assess the capability of biodegradation of toxicant by microbes in soil and sediment. Identifying biodegradative microorganisms is an important step in designing and analyzing soil bioremediation. To remove non-important noise information, it is necessary to selectively enrich genomes of biodegradative microorganisms fromnon-biodegradative populations. For this purpose, a stable isotope probing (SIP) technique was applied in selectively harvesting the genomes of biphenyl-utilizing bacteria from soil microbial communities. Since many biphenyl-using microorganisms are responsible for aerobic PCB degradation In soil and sediments, biphenyl-utilizing bacteria were chosen as the target organisms. In soil microcosms, 13C-biphenyl was added as a selective carbon source for biphenyl users, According to $13C-CO_2$ analysis by GC-MS, 13C-biphenyl mineralization was detected after a 7-day of incubation. The heavy portion of DNA(13C-DNA) was separated from the light portion of DNA (12C-DNA) using equilibrium density gradient ultracentrifuge. Bacterial community structure in the 13C-DNAsample was analyzed by t-RFLP (terminal restriction fragment length polymorphism) method. The t-RFLP result demonstates that the use of SIP efficiently and selectively enriched the genomes of biphenyl degrading bacteria from non-degradative microbes. Furthermore, the bacterial diversity of biphenyl degrading populations was small enough for environmental genomes tools (metagenomics and DNA microarrays) to be used to detect functional (biphenyl degradation) genes from soil microbial communities, which may provide a significant progress in assessing microbial capability of PCB bioremediation in soil and groundwater.

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Observation of Shape after sinter of Dental Co-Cr sinter metal (치과용 Co-Cr sinter metal의 소결 후 변형비교)

  • Song, Joon-Boo;Kim, Kap-Jin
    • Journal of Technologic Dentistry
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    • v.38 no.3
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    • pp.127-133
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    • 2016
  • Purpose: Currently advanced in Dental CAD/CAM technology increase a use of Co-Cr sinter metal block at Dental CAD/CAM part traditionally it has been being used with metal casting method. There is an advantage that can leave out a step of investment and casting as well as reducing a working time to compare with conventional casting method in a dry milling. Methods: In this research, compare a deformation of before and after sinter result of Co-Cr sinter metal block. Design a specimen figure by 3D CAD software and transformed it to a STL file can be used at Dental CAD/CAM. With a transformed STL file, milled a Co-Cr sinter metal specimen by using a Dental CAD/CAM dry milling machine. It arrived at the following conclusions after measuring an outer form and thickness of a machined specimen and then after sinter measure it again in a way of before sinter measurement method. Results: It is confirmed that there is no error while a 3D design figure specimen is transformed to a Dental CAD data. It is observed that there is no error at Co-Cr sinter metal specimen which consider a length of single coping and bridge. Conclusion: It is observed that there is no deformation at a specimen of AS05 and AB05 are made of 0,5mm thickness. On the other hand, the out of range of deformation is observed at a specimen of AS10 and AB10 which are made of 1mm thickness.

Development of Extra High Voltage(400kN) Porcelain Insulator for Transmission Lines (765 kV용 400 kN 현수애자 개발)

  • 최인혁;최장현;이동일;최연규
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.52 no.8
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    • pp.348-353
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    • 2003
  • This paper was the research of high voltage suspension insulator (400 [kN]) including pottery stone, feldspar, clay and alumina of 17 [wt%]. The slurry was fabricated after ball milling mixed raw materials. Green compacts were made by the extrusion of jiggering method and were sintered at 1300[$^{\circ}C$] for 50 [min.] in the tunnel kiln. The sintered density was reached to 97% of theoretical density, and the bending strength was 1658 [k $g_{f}$/$\textrm{cm}^2$] and hardness and fracture toughness which was measured by ICL( indentation crack length ) method were 1658 (kgf/$\textrm{cm}^2$) and 27.5 [Gpa], respectively. In measurement of tana and insulation break voltage of 400 (kN) porcelain, tan$\delta$ took some numerical value between 17${\times}$10$_{-3}$ and 61${\times}$10$_{-3}$ and insulation break voltage value was 19.9$\pm$1.4 [㎸/mm]. The test was performed to research whether the shape of pin affect a overvoltage break load or not As a consequence, when a pin was designed a pin diameter 51 [mm] with the bottom form of two-step constructed with straight in the suspension insulator, Insulator showed overvoltage break load 52 [ton] of the highest value and reflected a fine characteristic in aged deterioration test which is one of the accelerated aging test. Also it could be confirmed a fine characteristic through performing the test that electrical property of insulator was established correctly in accordance with IEC 60383-1 standards.s.

Properties of PP/MWCNT Nanocomposite Using Pellet-Shaped MWCNT (펠렛형 MWCNT를 사용한 PP/MWCNT 나노복합체 물성 연구)

  • Jeong, Dong-Seok;Nam, Byeong-Uk
    • Polymer(Korea)
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    • v.35 no.1
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    • pp.17-22
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    • 2011
  • Polypropylene/multi-walled carbon nanotube(PP/MWCNT) composites along with various MWCNT contents up to 20 wt% were prepared by a twin screw extruder. Nanocomposites having 20 wt% MWCNT as a master batch(M/B) were diluted with PP by way of melt compounding. The electrical/thermal conductivity, morphology, thermal/viscoelastic/mechanical properties were investigated with the variation of MWCNT contents. Also, we compared some properties between 1-step PP/MWCNT and the diluted PP/MWCNT composites. The percolation threshold of electrical and thermal conductivity was measured at about 3 wt% MWCNT. And conductivity of diluted PP/MWCNT composites were superior to those of PP/MWCNT composites. The non-isothermal crystallization temperature and thermal decomposition temperature appeared at higher temperatures with increasing MWCNT contents. Morphology showed that length of MWCNT in diluted PP/MWCNT composites was shortened by twice melt blending, which contributed to improve the tensile strength of PP/MWCNT composites.

The improvement of gait asymmetry ratio for hemiplegic patients by forceful respiratory exercise (노력성 호흡운동에 의한 편마비환자의 보행 비대칭율 개선)

  • Kim Byung-Jo;Lee Hyun-Ok;Ahn So-Youn
    • The Journal of Korean Physical Therapy
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    • v.16 no.4
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    • pp.38-58
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    • 2004
  • The regain of independent ambulatory ability is a important goal in the rehabilitation program of hemiplegic patient. Not only the function of lower extremity muscles, but also trunk muscles which stabilize extremities and pelvis, are important factors in normal gait. Therefor, it is necessary to develop an effective program which can improve muscle strength and symmetric activity of trunk muscles. The purpose of this study was to evaluate the influence of trunk muscle strengthening by forceful respiratory exercise on the gait asymmetry ratio in hemiplegic patient. 45 Hemiplegic patients due to stroke was randomized in 3 groups, forceful expiratory training(FET), forceful inspiratory training(FIT) and control group. In the experimental groups, ordinary physical therapy with forceful expiratory training and forceful inspiratory training for 20 minutes duration 3 times per week for 6 weeks were respectively performed. In the control group, only ordinary physical therapy was done. Before and after experiments, temporal-spatial gait parameters was measured in all patients. The data of 28 patients who carried out the whole experimental course were statistically analysed. The results of these experiment are as follows : 1. In comparison of difference of single support time asymmetry ratio among 3 groups, the FET group was significantly decreased than the control group (p<.05). 2. In comparison of difference of step length asymmetry ratio among 3 groups, the FIT group was significantly decreased than the control group (p<.05). Based on these results, it is concluded that the forced respiratory exercise program for 6 weeks can be improve the gait asymmetry ratio in hemiplegic patients. Therefore, the forced respiratory exercise is useful to improve the walking ability in hemiplegic patients. Since this study dealt only with the patients who could walk more than 3 meters in distance on floor independently, the further study for evaluating the influence of the forceful respiratory exercise on patients with acute stage stroke and also the development in various methods of use are expected.

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Curvature Linear Equation of a Coma Corrected Two-Mirror System with Finite Object Distance (유한 물체거리를 갖는 코마수차가 보정된 2 반사경계의 곡률선형방정식)

  • Hwang, Seok-Ju;Rim, Cheon-Seog;Jo, Jae-Heung
    • Korean Journal of Optics and Photonics
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    • v.18 no.1
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    • pp.19-23
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    • 2007
  • We derived analytically the generalized curvature linear equation useful in the initial optical design of a two-mirror system with finite object distance, including an infinite object distance from paraxial ray tracing and Seidel third order aberration theory for coma coefficient. These aberration coefficients for finite object distance were described by the curvature, the inter-mirror distance, and the effective focal length. The analytical equations were solved by using a computer with a numerical analysis method. Two useful linear relationships, determined by the generalized curvature linear equations relating the curvatures of the two mirrors, for the cancellation of each aberration were shown in the numerical solutions satisfying the nearly zero condition ($<10^{-10}$) for each aberration coefficient. These equations can be utilized easily and efficiently at the step of initial optical design of a two-mirror system with finite object distance.

Potential Use of a Smartphone to Evaluate Gait during Walking in Stroke Patients (스마트폰 어플리케이션을 이용한 뇌졸중 환자의 보행 평가 가능성)

  • An, Bo-Ra;Ki, Kyong-Il;Woo, Young-Keun
    • PNF and Movement
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    • v.16 no.1
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    • pp.67-73
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    • 2018
  • Purpose: Smartphones, which are widely used worldwide to detect acceleration and position, have been used in the area of rehabilitation medicine in recent clinical research studies and tests. The aim of the present study was to determine the feasibility of using a smartphone application based on center of movement (COM) displacement to measure gait parameters in stroke patients in the clinical field of rehabilitation medicine. Methods: The study consisted of 30 stroke patients. The COM was measured using a smartphone application, Gait Analysis Pro, during a 6-m walk. Each patient performed three 6-m walking trials, and the smartphone application measured gait duration, gait speed, step length, cadence, and vertical and lateral displacement of the COM. The Kolmogorov-Smirnov test was conducted to determine the normality in gait parameters, and a repeated one-way analysis of variance (ANOVA) was performed to determine the consistency among the three trials. A p value of 0.05 was considered statistically significant in all the tests. Results: In all the measured parameters, the smartphone application showed a normal distribution, as shown by the results of the Kolmogorov-Smirnov test. There were no significant differences among the three repetitive walking trials. Conclusion: These results suggest that the smartphone application can be used for evaluating gait in stroke patients, as well as in healthy adults. However, prior to using the smartphone application in the clinical field, further research involving three-dimensional gait analysis is needed to enhance the confidence level of the findings.

Variations of NO Concentration Released from Fertilized Japanese Upland Soil Under Different Soil Moisture Conditions

  • Kim, Deug-Soo;Haruo Tsuruta;Kazuyuki Inubushi
    • Journal of Korean Society for Atmospheric Environment
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    • v.14 no.E
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    • pp.9-17
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    • 1998
  • Oxides of nitrogen play important roles in atmospheric chemistry. Soil has been recognized as a major natural source of NO, and its emission depends on soil parameters such as soil nitrogen availability, soil moisture and temperature. It is necessary to understand effects of these controlling parameters on soil NO emission. In order to understand soil moisture effects on NO emission, variations of NO concentration and existence of its equilibrium concentration were observed from ammonium fertilized Japanese upland soil prepared for different soil moisture conditions. The closed chamber technique was employed for this study. The significant increases in NO with soil moisture were found. Maximum was occurred at sample ID4 (55% of water-filled pore space (WFPS)), but it decreased as soil moisture increased. No significant NO concentration was emitted from soil sample without fertilizer, but there was significant NO in fertilized soil samples. The magnitudes of NO from soil increased with time and reached at steady state within ten minutes approximately. These results suggest that nitrogen input from fertilizer takes charge in the first step of sharp increase in NO emission, and then soil moisture becomes important factor to control NO emission from the soils. NO concentrations from soil were compared to those one-day after the experiment. Results from the comparison analysis suggest that the soil NO flux might have been stimulated by soil disturbances like mixing, and this is much more effective in dry soils rather than in wet soils. It was found that much less NO came out from soils after a day; suggesting that most of NO was released from the soils within a day after fertilizer application during our experiment. The length of NO releasing time span may depend on the amounts of fertilizer applied, soil moisture condition, and other soil physical parameters.

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A Study on Heat Transfer Characteristics of a Closed Two-Phase Thermosyphon with a Low Tilt Angle (낮은 경사각을 갖는 밀폐형 2상 열사이폰의 열전달 특성에 관한 연구)

  • 김철주;강환국;김윤철
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.8 no.1
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    • pp.1-12
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    • 1996
  • In lots of application to heat exchanger systems, closed two-phase thermosyphons are tilted from a horizontal. If the tilt angle, especially, is less than 30$^{\circ}$, the operational performances of thermosyphon are highly dependent on tilt angle. The present study was conducted to better understand such operational behaviors as mech-anni는 of phase change, and flow patterns inside a tilted thermosyphon. For experiment, an ethanol thermosyphon with a 35% of fill charge rate was designed and manufactured, using a copper tube with a diameter 19mm and a length 1500mm. Through a series of test, the tilt angle was kept constant at each of 4 different values in the range 10~25deg. and the heat supply to the evaporator was stepwisely increased up to 30㎾/$m^2$. When a steady state was established to the thermosyphon for each step of thermal loads, the wall temperature distribution and vapor temperature at the condenser were measured. The wall temperature distributions demonstrated a formation of dry patch in the top end zone of the evaporator, with a values of temperature 20~4$0^{\circ}C$ higher than the wetted surface for a moderate heat flux q≒20㎾/$m^2$. Inspite of the presence of hot dry patch, however, the mean values of boiling heat transfer coefficient at the evaporator wall were still in a good agreement with those predicted by Rohsenow's formula, which was based on nucleate boiling. For the condenser, the wall temperatures were practically uniform, and the measured values of condensation heat transfer coefficient were 1.7 times higher than the predicted values obtained from Nusselt's film condensation theory on tilted plate. Using those two expressions, a correlation was formulated as a function of heat flux and tilt angle, to determine the total thermal resistance of a tilted thermosyphon. The correlation formula showed a good agreement with the experimental data within 20%.

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A Study on Change in Gait Parameters Related to the Cop and Velocity Resulting from the Use of Ankle Foot Orthosis in Spastic Diplegia (경직성 양하지마비아의 발목 보조기 착용에 따른 체 중심및 보행속도에 의한 보행인자 변화연구)

  • Park, Mi-Sook;Kim, Yong-Seong;Hwang, Tae-Yeon;Kim, Yong-Nam
    • Journal of the Korean Academy of Clinical Electrophysiology
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    • v.10 no.1
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    • pp.23-28
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    • 2012
  • Purpose : The objectives of this study were to examine changes in gait parameters related to the COP and gait velocity resulting from the use of ankle foot orthosis, and to provide information for the prescription of ankle foot orthosis and gait rehabilitation training. Methods : We sampled a control group of 14 normal children of the same age as the spastic diplegia (spastic diplegia used ankle foot orthosis) and measured their COP at barefoot gait and their gait parameters at three gait velocities. Change in the COP according to the use of ankle foot orthosis measured one-way ANOVA and Gait parameters according to gait velocity used repeated measures ANOVA. Results : As a result of analyzing change in the COP it became close to that of normal children, and changes in gait parameters such as step width, stride length and stride time also became close to those of normal children. Conclusion : In conclusion, when spastic diplegia used ankle foot orthosis, their balancing ability was improved as a result of decreased change in the COP, and their changes in gait parameters also became close to those of the control group. These results show that the use of ankle foot orthosis improves spastic diplegia gait functions.