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Effect of Application Level of Animal Manure on the Nitrate Concentration, Sugar Content and Intake of Forage Sorghum X Sudangrass Hybrid (가축분 시용수준이 수수 X 수단그라스 교잡종의 질산태질소 함량과 당도 및 채식률에 미치는 영향)

  • Seo, S.;Kim, J.G.;Chung, E.S.;Kim, W.H.;Choi, G.J.;Lee, J.K.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.2
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    • pp.123-130
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    • 2002
  • A field experiment was carried out to determine the effect of application level of animal manure on the nitrate nitrogen concentration, sugar content and animal intake of forage sorghum $\times$ sudangrass hybrid (Sorghum bicolor (L.) Moench, cv. Pionee. 988) in 1995. The application amount of animal manure were 50, 100 and 150MT in cattle manure, 20, 40 and 80MT in swine manure, and 10, 20 and 40MT/ha in poultry manure. Non-application plot(control) was involved. The nitrate nitrogen concentration was increased with increasing of application level of animal manure(P<0.05). Average nitrate nitrogen concentration was 397, 512, and 609mg/kg at low, medium and high application level of animal manure. The nitrate nitrogen concentration by plant height was 438mg/kg at 50~60m of plant height, 454mg at 100~120cm, and 418mg at 200~220cm. The nitrate nitrogen concentration of stems was 376mg, and significantly higher than that(135mg) of leaves(P<0.05) regardless of animal manure type, and lower parts of stems and leaves were significantly higher than those of upper parts of plants(P<0.05). Average nitrate nitrogen concentration of leaves was 151mg at lower, and 58mg at upper parts of plants, and the concentration of stems was 357mg at lower, 511mg at middle, and 610mg at upper parts of plants. The sugar contents of sorghum $\times$ sudangrass hybrid was decreased with increasing of application level of animal manure(P<0.05). Average sugar content was 4.9, 4.4, and 4.3。 at low, medium and high application level of animal manure. The sugar content by plant height was 3.9。 at 50~60 and 100~120cm of plant height, and 6.1。 at 200~220cm of plant height. Animal intake of sorghum $\times$ sudangrass hybrid was decreased greatly with increasing of application level of animal manure. Average intake was 73.9, 55.7, and 52.3% at low, medium and high application level of animal manure. The intake by animal manure type was 73.7% in cattle, 59.7% in swine and 62.5% in poultry manure.

Vegetation Change of Long-Term Ecological Research (LTER) Site of Abies koreana Forests in Hallasan Mountain (Yeongsil Area) (한라산(영실) 장기생태연구(LTER) 조사지 구상나무 숲 식생 변화)

  • Song, Kuk-Man;Kim, Jae-Hoon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.21 no.1
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    • pp.1-12
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    • 2018
  • This study sought to analyze changes in the vegetation structure and species diversity indices in the Abies koreana forest in the Yeongsil area of Hallasan Mountain and establish basic data for Long-Term Ecological Research (LTER). The results show that the number of woody plants per 1ha has increased by 804 (45.1%) from 1,781 to 2,585 individual plants, whereas the number of live A. koreana did not change much with 796 individual plants. The number of dead tree plants, however, jumped by 807 (82.2%) from 982 to 1,789 plants. With other tree species, the number of live individual plants increased by 807 (82.2%) from 982 to 1,789 plants, whereas the number of dead plants increased by 31 from 10 to 41 individual plants. Results of the average importance index analysis show that the number of A. koreana decreased, while the number of Weigela subsessilis, Rhododendron yedoense for. poukhanense, and Magnolia sieboldii increased. The species diversity index increased by 6.7% as per the maximum level in the upper layer and by 7.2% in the lower layer, respectively. Results of the stem morphology analysis show that the number of AS type was highest with 700 individual plants among A. koreana, while their distribution by type shifted from AS>AL>DS>DB to AS>DS>AL>DB. As for other species, the AS type was most abundant with 1,580 individual plants, while their distribution by type shifted from AL>AS>AF>AB to AS>AL>AB>AD. The number of newly sprouted tree plants has increased from 420 in 2014 to 453 in 2016. Of the individual plants that were alive in 2014, 23 were found dead and 33 individual plants younger than 3 years old have been newly added to the group. The number of deaths after 2014 was highest among the 2 years olds, whereas the number of 2 years olds that sprouted in 2014 was highest among the newly sprouted tree plant group. In the A. koreana forest in Yeongsil, the density of other species was rising rapidly with the exception of the dead and live A. koreana. The occurrence of new tree plants is decreasing rapidly, thereby confirming the rapid change in the area's vegetation structure. Therefore, there is a need for continuous long-term ecological research to monitor vegetation change. It can also be used as a basic data for efficient restoration research.

Screening of Useful Plants with Anti-inflammatory and Antioxidant Activity (항염증 및 항산화 활성 보유 유용 식물 탐색)

  • Lee, Seung-Eun;Choi, Jehun;Lee, Jeong-Hoon;Noh, Hyung-Jun;Kim, Geum-Sook;Kim, Jinkyung;Chung, Hae-Young;Kim, Seung-Yu
    • Korean Journal of Plant Resources
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    • v.26 no.4
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    • pp.441-449
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    • 2013
  • This study was conducted to select some useful plants as functional material candidates. A total of 38 plants were preliminarily screened for the anti-inflammatory and antioxidant activities. The preliminarily selected 8 plants were further investigated to verify the in vitro inhibitory effect on inflammation and oxidative stress. Boehmeria platanifolia (root), Carpinus coreana (branch), and Eupatorium japonicum (leaf) inhibited the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-treated RAW 264.7 cells. Eupatorium japonicum (leaf) suppressed the expression of cyclooxygenase-2 (COX-2), whereas Boehmeria platanifolia (root) and Prunus yedoensis (branch) inhibited the transcription of nuclear factor-kappa B (NF-${\kappa}B$). Treatment with the extracts ($2.5{\sim}20{\mu}g/ml$) of Abutilon theophrasti (leaf, flower/seed) and Hemistepta lyrata (stem) did not show toxicity on RAW 264.7 cell proliferation, but treatment with $2.5{\mu}g/ml$ of Boehmeria platanifolia (root) exhibited cell toxicity. Carpinus coreana (branch) and Prunus yedoensis (branch) showed potent scavenging activities on peroxynitrite. Akebia quinata (flower), Carpinus coreana (branch), and Prunus yedoensis (branch) effectively inhibited reactive oxygen species (ROS). Abutilon theophrasti (leaf), Boehmeria platanifolia (root), Carpinus coreana (branch), and Eupatorium japonicum (leaf) exhibited strong inhibitory capacity with regard to nitric oxide (NO) production. The results suggested that Abutilon theophrasti (leaf) has in vitro anti-inflammatory and antioxidant activities, and that is a useful functional material candidate.

Selective Mechanism of Cyhalofop-butyl ester between Rice and Echinochloa crus-galli - I. Differential Response of Rice and Echinochloa crus-galli to Cyhalofop-butyl ester (제초제(除草劑) Cyhalofop-butyl ester의 벼와 피간(間) 선택성기작(選擇性機作) - I. 제초제(除草劑) Cyhalofop-butyl ester에 대한 벼와 피간(間)의 반응차이(反應差異))

  • Park, J.E.;Ryu, G.H.;Lee, I.Y.;Lee, H.K.;Shin, H.S.;Lee, J.O.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.14 no.2
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    • pp.94-100
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    • 1994
  • This experiment was conducted to determine selective mechanism of cyhalofop-butyl ester ((((R-butyl 2-(4-(4-cyano-2-fluorophenoxy) phenoxy) propionate)) between rice and Echinochloa crus-galli. 100ppm of cyhalofop-butyl ester inhibited over 90% of seedling growth of E. crus-galli when applied at 3 leaf stage and complete inhibition was observed at 180ppm applied at the 4 leaf stage, but rice(Chucheongbyeo) was not inhibited by cyhalofop-butyl ester even at 230ppm, regardless of its growth stages(3, 4, 5 and 6 leaf stages). Cyhalofop-butyl ester applied through stem at 10 and 50ppm moved most rapidly to the meristem and resulted in the highest injury on plant height, root length and fresh weight of E. crus-galli. compared with root or leaf application. Seedlings of rice and E. crus-galli at 3 or 4 leaf stage were dipped in 180ppm of cyhalofop-butyl ester solution for 1 minute and aboveground parts of E. crus-galli and rice were removed immediataly after dipping treatment. Regrowth of E. crus-galli was inhibited by the herbicide by 41.7%, but no inhibition was observed in rice. Further, content of chlorophyll reduced to 18.7% of the untreated control, showing appearence of almost being killed, but no effect on chlorophyll content of rice was observed.

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Evaluation of Agronomic Characteristics and Fatty Acid Composition of Flax Germplasms (아마 유전자원의 농업적 특성 및 지방산 조성 평가)

  • Lee, Yong-Hwa;Bae, Sang-Mok;Park, Won;Kim, Kwang-Soo;Jang, Young-Seok;Lee, Kyeong-Bo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.61 no.2
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    • pp.124-130
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    • 2016
  • In this study, agronomic characteristics and fatty acid composition of 121 flax (Linum usitatissimum L.) germplasms were analyzed. The flax germplasms had a fairly short growth period of 75-95 days. The plant height at maturity varied from 52 to 124 cm. The flower color varied from white to lilac and even purple. Early flowering flax germplasms had white flowers and were resistant to lodging. In early flowering flax germplasms, all agronomic characteristics, including growth period, plant height, branch number per plant, stem diameter, number of capsules per raceme, seed number per capsule, 1000-seed weight, and seed yield, were decreased. The average fatty acid composition of 121 flax germplasms comprised palmitic acid (5.3%), stearic acid (4.3%), oleic acid (29.7%), linoleic acid (13.1%) and linolenic acid (46.4%). The total content of unsaturated fatty acids ranged from 84~94% with an average of 88%. Among the fatty acids, linolenic acid (omega-3) content was the highest with a range of 36~54%. In five selected flax germplasms (Hollandia 1803, Red son, C & F Res Br 1767, Wiera, and Ireland 1657) with high linolenic acid (${\geq}51%$), total lipid and protein contents were 30~36% and 5.4~6.9%, respectively. Overall, flax can practically be adoptable into cropping systems in South Korea and utilized as a scenery crop, since flax has a short cultivation period with diverse flower colors.

Changes in Tomato Growth and Productivity under Different Night Air Temperatures (야간온도에 따른 토마토 생육 및 생산성 변화)

  • Kang, Yun-Im;Kwon, Joon-Kuk;Park, Kyoung-Sub;Choi, Gyeong-Lee;Roh, Mi-Young;Cho, Myeong-Whan;Kim, Dae-Young;Kang, Nam-Jun
    • Journal of agriculture & life science
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    • v.46 no.6
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    • pp.25-31
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    • 2012
  • This study aimed to investigate the effect of night temperature on the productivity, growth, and fruit characteristics of tomatoes and set the optimal night air temperature. Tomatoes (Solanum lycopersicum Mill. cv. Superdoterang and cv. Rapito) were grown at 5, 10, 15, and $20^{\circ}C$ during night time and ventilation temperature point during day time set $27^{\circ}C$. Depending on night air temperature, plant height, leaf area, fresh and dry weight of each organ, leaf, stem, and root were changed. Condition of high night temperature resulted in promotion of tomatoes growth with high dry weight and fresh weight. However, the dry weight under night temperature $20^{\circ}C$ decreased, especially, in 'Superdoterang'. On changes of yield, 'Rapito' showed higher productivity than 'Superdoterang' and the highest productivity point for nigh temperature is $13.32^{\circ}C$ for 'Superdoterang' and $14.25^{\circ}C$ for 'Rapito'. Although the total yield decreased from the point, the daily productivity increased with increasing night temperature, the highest point for nigh temperature is $14.45^{\circ}C$ for 'Superdoterang' and $16.46^{\circ}C$ for 'Rapito'. High temperature deceased the fruit weight and increased total soluble solid content in fruits. It is concluded that the night air temperature changes productivity of tomato and the temperatures between productivity and growth velocity have differences depending on different cultivars. scores than the other beef jerky samples (p<0.05).

Effect of Soil Mulching Materials and Methods on Weed Occurring for the Growth and Flowering in Gypsophila paniculata Cultivation (토양피복에 따른 잡초발생과 안개초의 생육 및 개화에 미치는 영향)

  • Cheong, Dong-Chun;Oh, Jeong-Moon;Lim, Hoi-Chun;Song, Young-Ju;Kim, Jeong-Man
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.1
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    • pp.15-21
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    • 2011
  • This study investigated the effect of soil mulching materials and methods on weed occurring for the growth and flowering characteristics in Gypsophila plants cultivation. For mulching materials were used black polyethylene (PE) film, black polypropylene (PP), and green polypropylene, and these were mulched in both ridge and furrow (BRF) or only furrow, respectively. The weed was occurred total $146.2g/m^2/yr$ as dry weight in non-mulching, but $4.1{\sim}4.2g/m^2/yr$ in BRF mulching by black or green PP. Also weed control were yearly required 27.9 persons/10a in non-mulching, whereas 2.4 persons/10a in BRF mulching by black or green PP. Flower budding and blooming were roughly delayed in non-mulching and furrow mulching. Flowering characteristics such as flower stalk length, primary branches number, and stem firmness were remarkably improved in BRF mulching of black or green PP. Cut flower yield was increased on black BRF mulching or green PP in summer cultivation, while green PP BRF mulching showed better yield in in autumn cultivation. Mortality rate of Gypsophila plants after summer season was lowest as 12.4% in furrow mulching with black PP, followed by about 19.0% in green PP mulching, but highest as 39.0% in BRF mulching with black PE film. Rosette formation rate was lower in furrow than BRF mulching, and was lowest as 13.1% in BRF mulching with green PP, followed by 15.2~15.8% in BRF mulching with black PE film or black PP. So it was thought that BRF mulching with green PP was highly effective in weed control and improvement of yield and cut flower quality in Gypsophila plants cultivation.

Occurrence and Damage of Golden Apple Snail (Pomacea canaliculata : Ampullariidae) in Jeonnam Province of South Korea (전남지역 왕우렁이(Pomacea canaliculata: Ampullariidae)의 발생생태 및 피해)

  • Kim, Do-Ik;Kim, Seon-Gon;Choi, Kyeong-Ju;Kang, Beom-Ryong;Park, Jong-Dae;Kim, Jeong-Jun;Choi, Dong-Ro;Park, Hyeong-Man
    • Korean journal of applied entomology
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    • v.46 no.1 s.145
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    • pp.109-115
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    • 2007
  • This study was carried out to determine occurrence and damage of golden apple snail, Pomacea canaliculata on water seeding area in Jeonnam province. The density of golden apple snail maintained hlgh until October 2003, but quickly decreased from November. They overwintered only in a portion of the water canals but could not find in dried paddy field. They reproduced from May and June in the canal water and paddy field, respectively. Feeding time of golden apple snail on rice seedlings was 235.2 seconds, feeding length of that was 8.4cm. When golden apple snail fed rice seedlings, the stem remain only 2.5cm from areal part which could not recover. The more density of golden apple snail, the fester feeding rate. Density and number of eggs laid of golden apple snail after overwintering was higher on bank around of paddy field than on middle of that. The percent of shell size after over wintering was higher in small (below 25mm) than big (over 36mm). But in case of Boseong area, the slze was reverse because the soil has soft mud. The survival rate of overwintering golden apple snail was lower than that of breeding. They did not move when the temperature changed rapidly, but the temperature increased they start to move. They dead almost below $-3^{\circ}C$. The damage of rice by golden apple snail in the direct water seeding was 20%. In machine transplanting, rice damaged only fore-end of leaf and recovered subsequently. When golden apple snail release in direct water seeding field, the percent of damage was 5.6% in immediately release plot. The later release, the lower damage.

EVALUATION OF OSTEOGENIC ACTIVITY AND MINERALIZATION OF CULTURED HUMAN PERIOSTEAL-DERIVED CELLS (배양된 인간 골막기원세포의 조골활성 및 골기질 형성의 평가)

  • Park, Bong-Wook;Byun, June-Ho;Lee, Sung-Gyoon;Hah, Young-Sool;Kim, Deok-Ryong;Cho, Yeong-Cheol;Sung, Iel-Yong;Kim, Jong-Ryoul
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.28 no.6
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    • pp.511-519
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    • 2006
  • Autogenous bone grafts have been considered the gold standard for maxillofacial bony defects. However, this procedure could entail a complicated surgical procedure as well as potential donor site morbidity. Possibly the best solution for bone-defect regeneration is a tissue engineering approach, i.e. the use of a combination of a suitable scaffold with osteogenic cells. A major source of osteogenic cells is the bone marrow. Bone marrow-derived mesenchymal stem cells are multipotent and have the ability to differentiate into osteoblastic, chondrocytic, and adipocytic lineage cells. However, the isolation of cells from bone marrow has someproblems when used in clinical setting. Bone marrow aspiration is sometimes potentially more invasive and painful procedure and carries of a risk of morbidity and infection. A minimally invasive, easily accessible alternative would be cells derived from periosteum. The periosteum also contains multipotent cells that have the potential to differentiate into osteoblasts and chondrocytes. In the present study, we evaluated the osteogenic activity and mineralization of cultured human periosteal-derived cells. Periosteal explants were harvested from mandibule during surgical extraction of lower impacted third molar. The periosteal cells were cultured in the osteogenic inductive medium consisting of DMEM supplemented with 10% fetal calf serum, 50g/ml L-ascorbic acid 2-phosphate, 10 nmol dexamethasone and 10 mM -glycerophosphate for 42 days. Periosteal-derived cells showed positive alkaline phosphatase (ALP) staining during 42 days of culture period. The formation of ALP stain showed its maximal manifestation at day 14 of culture period, then decreased in intensity during the culture period. ALP mRNA expression increased up to day 14 with a decrease thereafter. Osteocalcin mRNA expression appeared at day 7 in culture, after that its expression continuously increased in a time-dependent manner up to the entire duration of culture. Von Kossa-positive mineralization nodules were first present at day 14 in culture followed by an increased number of positive nodules during the entire duration of the culture period. In conclusion, our study showed that cultured human periosteal-derived cells differentiated into active osteoblastic cells that were involved in synthesis of bone matrix and the subsequent mineralization of the matrix. As the periosteal-derived cells, easily harvested from intraoral procedure such as surgical extraction of impacted third molar, has the excellent potential of osteogenic capacity, tissue-engineered bone using periosteal-derived cells could be the best choice in reconstruction of maxillofacial bony defects.

Reduction of Bacterial Wilt Diseases with Eggplant Rootstock EG203-Grafted Tomatoes in the Field Trials (가지대목 EG203을 이용한 토마토 풋마름병 경감효과)

  • Lee, Mun Haeng;Kim, Ji Kwang;Lee, Hee Kyoung;Kim, Keyng Jae;Yu, Seung Hun;Kim, Young Shik;Lee, Youn Su
    • Research in Plant Disease
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    • v.19 no.2
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    • pp.108-113
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    • 2013
  • Wilt damage on tomato plants caused by Ralstonia solanacearum has been increased as the areas of tomato cultivation increased during the warm seasons. Also, the tomato rootstocks used to prevent the disease occurrence are not effective in the highly prevailing regions. Therefore, bacterial wilt resistant eggplant rootstock EG203, collected from AVRDC, was tested for its effect to deter the Ralstonia solanacearum wilt disease in the greenhouses at Buyeo Tomato Experiment Station from 2003 to 2005, and at Gumi, Kyungpook province from 2009-2011. Planting of eggplant rootstock EG203 should be done three weeks before the planting of tomato scions so that they can have similar stem diameter (2.5-3.0 mm) and can be easily grafted. Both insertion and inarching grafting showed 93-96% success rates. In the greenhouse tests at Buyeo Tomato Experiment Station from 2003 to 2005, eggplant rootstock EG203-grafted tomatoes showed the disease occurrence of 4.3%. On the other hand, non-grafted or other commercial rootstock-grafted tomatoes showed disease occurrence of 58.0% and 25.0-36.7%, respectively. In the greenhouse tests at Gumi, Kyungpook province in 2009, the disease occurrence on the EG203-grafted and non-grafted tomatoes was 2-5% and 20-80%, respectively. In 2010, at Gumi, Kyungpook province, when the wilt disease occurred slightly, the tomatoes grafted with tomato rootstocks B-blocking and Chung-gang, and eggplant rootstock EG203 showed similar disease severities, but EG203-grafted tomatoes formed lately cluster, resulting in the reduction of yield compared to tomato-grafted tomatoes. In 2011, at Gumi, Kyungpook province, when the wilt disease occurred severely, the tomato rootstocks 'B-blocking' and Chung-gang and eggplant rootstock EG203-grafted tomatoes showed disease occurrences of 60-85% and 0-1%, respectively. Therefore, it was concluded that tomato rootstocks 'B-blocking' and 'Chung-gang' are more useful in the areas contaminated with low levels of pathogen and eggplant rootstock EG203 is more useful in the areas contaminated with high levels of pathogen.