• Title/Summary/Keyword: STEM research

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A Study on the Effects of a Virtual-Users Model Computing the Semantics of Spaces for the Operation and Understanding of Human Behavior Simulation of Architecture-Major Students (공간의 의미를 연산하는 가상 사용자 모델이 건축설계 전공학생들의 인간행동 시뮬레이션 운용과 이해도에 미치는 효과에 관한 연구)

  • Hong, Seung-Wan
    • Journal of KIBIM
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    • v.6 no.3
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    • pp.34-41
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    • 2016
  • The previous studies argue that using the semantic properties of BIM objects is efficient for simulating the behaviors of autonomous, computer agents, called virtual-users, but such assumption is not proven via evidence-based research approaches. Hence, this present study aims to investigate the empirical effects of a human behavior simulation model equipped the semantics of spaces on the architecture-major students' operation and understanding of the simulation system, compared to a typical path-finding model. To achieve the aim, this study analyzed the survey and interview data, collected in the authentic design projects. The analysis indicates that (1) using a simulation model equipped the semantics of spaces helps the students' operation of the simulation, and (2) it also aids understanding the relationship between the variables of spaces and virtual-users (${\alpha}=0.74$). In addition, the qualitative data inform that the advantages of the simulation model that computes the semantics of spaces stem in the automatic behavioral changes of massive numbers of virtual-users, and efficient detection and activation on the what-if situations. The analysis also reveals that the simulation model has shortcomings in orchestrating the complex data structure between the semantics properties of spaces and virtual-users under multi-sequential scenarios. The results of this study contribute to develop a future design system combining BIM with human behavior simulation.

Cloning, Nucleotide Sequencing, and Characterization of the ptsG Gene Encoding Glucose-Specific Enzyme II of the Phosphotransferase System from Brevibacterium lactofermentum

  • Yoon, Ki-Hong;Lee, Kyu-Nam;Lee, Jung-Kee;Park, Se-Cheol
    • Journal of Microbiology and Biotechnology
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    • v.9 no.5
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    • pp.582-588
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    • 1999
  • A Brevibacterium lactofermentum gene coding for a glucose-specific permease of the phosphoenolpyruvate-dependent phosphotransferase system (PTS) was cloned, by complementing an Escherichia coli mutation affecting a ptsG gene with the B. lactofermentum genomic library, and completely sequenced. The gene was identified as a ptsG, which enables an E. coli transformant to transport non-metabolizable glucose analogue 2-deoxyglucose (2DG). The ptsG gene of B. lactofermentum consists of an open reading frame of 2,025 nucleotides encoding a polypeptide of 674 amino acid residues and a TAA stop codon. The 3' flanking region contains two stem-loop structures which may be involved in transcriptional termination. The deduced amino acid sequence of the B. lactofermentum enzyme $II^{GIe}$ specific to glucose ($EII^{GIe}$) has a high homology with the Corynebacterium glutamicum enzyme $II^{Man}$ specific to glucose and mannose ($EII^{Man}$), and the Brevibacterium ammoniagenes enzyme $II^{GIc}$ specific to glucose ($EII^{GIc}$). The 171-amino-acid C-terminal sequence of the $EII^{Glc}$ is also similar to the Escherichia coli enzyme $IIA^{GIc}$ specific to glucose ($IIA^{GIc}$). It is interesting that the arrangement of the structural domains, IIBCA, of the B. lactofermentum $EII^{GIc}$ protein is identical to that of EIIs specific to sucrose or $\beta$-glucoside. Several in vivo complementation studies indicated that the B. lactofermentum $EII^{Glc}$ protein could replace both $EII^{ Glc}$ and $EIIA^{Glc}$ in an E. coli ptsG mutant or crr mutant, respectively.

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Embryogenic callus culture of Tribulus terrestris L. a potential source of harmaline, harmine and diosgenin

  • Nikam, T.D.;Ebrahimi, Mohammad Ali;Patil, V.A.
    • Plant Biotechnology Reports
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    • v.3 no.3
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    • pp.243-250
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    • 2009
  • In the present study, a simple one medium formulation protocol for callus culture, somatic embryogenesis and in vitro production of ${\beta}-carboline$ alkaloids and diosgenin in Tribulus terrestris L. was developed. Extensive callus induction and proliferation was obtained in leaf explant on Murashige and Skoog (MS) medium supplemented with $5.0{\mu}M$ 6 benzyl adenine (BA) and $2.5{\mu}M$ ${\alpha}-naphthaleneacetic$ acid (NAA). The embryogenic callus was maintained on subculture to fresh parental medium at 4-week intervals over a period of 28 months. The frequency of embryo formation was at a maximum ($18.1{\pm}0.9$ per g of callus) on MS medium containing $5.0{\mu}M$ BA and $2.5{\mu}M$ NAA together with $75mg\;1^{-1}$ casein hydrolysate. Globular embryo developed into torpedo stage embryo under the influence of starvation. The accumulation of ${\beta}-carboline$ alkaloids (harmaline and harmine) and steroidal saponin (diosgenin) in non-embryogenic and embryogenic callus culture derived from leaf explant was compared with root, leaf, stem, and fruit of the mother plant. The embryogenic callus accumulated equivalent amounts of harmaline ($66.4{\pm}0.5{\mu}g/g$ dry weight), harmine ($82.7{\pm}0.6{\mu}g/g$ dry weight), and diosgenin ($170.7{\pm}1.0{\mu}g/g$ dry weight) to that of the fruit of T. terrestris. The embryogenic callus culture of this species might offer a potential source for production of important pharmaceuticals.

Deep Learning-based Rice Seed Segmentation for Phynotyping (표현체 연구를 위한 심화학습 기반 벼 종자 분할)

  • Jeong, Yu Seok;Lee, Hong Ro;Baek, Jeong Ho;Kim, Kyung Hwan;Chung, Young Suk;Lee, Chang Woo
    • Journal of Korea Society of Industrial Information Systems
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    • v.25 no.5
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    • pp.23-29
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    • 2020
  • The National Institute of Agricultural Sciences of the Rural Developement Administration (NAS, RDA) is conducting various studies on various crops, such as monitoring the cultivation environment and analyzing harvested seeds for high-throughput phenotyping. In this paper, we propose a deep learning-based rice seed segmentation method to analyze the seeds of various crops owned by the NAS. Using Mask-RCNN deep learning model, we perform the rice seed segmentation from manually taken images under specific environment (constant lighting, white background) for analyzing the seed characteristics. For this purpose, we perform the parameter tuning process of the Mask-RCNN model. By the proposed method, the results of the test on seed object detection showed that the accuracy was 82% for rice stem image and 97% for rice grain image, respectively. As a future study, we are planning to researches of more reliable seeds extraction from cluttered seed images by a deep learning-based approach and selection of high-throughput phenotype through precise data analysis such as length, width, and thickness from the detected seed objects.

A New Spray Chrysanthemum Cultivar 'Yes Line' with Single Type and Purple Color (자주색 홑꽃 스프레이국 신품종 'Yes Line' 육성)

  • Pak, Ha-Seung;Choi, Taeg-Yong;Won, Mi-Kyoung;An, Jong-Beom
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.4
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    • pp.317-320
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    • 2008
  • A new spray chrysanthemum(Dendranthema grandiflorum Ramat.) cultivar 'Yes Line' was originated from a cross between 'T22319' and 'SP02-037-13'. This cultivar was tested on the characteristics from 2004 to 2007 for the evaluation and selection including shading and retarding culture. The natural flowering time of 'Yes Line' is late October, but year-round production is possible by shading, lighting and retarding culture. This cultivar was single in flower type, with a color of red-purple(RHS 74C), with the flowers of 21.4 per stem. 'Yes Line' showed the vase life of 16.5 days in autumn season. The diameters of flower and flower center were 4.3 cm, 1.2 cm respectively. The days to flower under the short day treatment was 7.5 weeks and preference of consumers were high at 3.86 ranged up to 5.00. It has resistance to white rust and was possible to cultivate all-year-round.

'Arirang', Early-flowering Pure White Gladiolus with Purple Core (조생 순백색 자주 화심 글라디올러스 '아리랑')

  • Cho, Hae Ryong;Rhee, Hye Kyung;Lim, Jin Hee
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.2
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    • pp.142-146
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    • 2010
  • 'Arirang', an early-flowering pure white gladiolus with purple core, was released by the National Institute of Horticultural & Herbal Science (NIHHS) in 1998. The selection was derived from the cross between the progenies of the early flowering dark orange 'Arianne' and bright red domestic cultivars 'Hongkwang'. The breeding was conducted in 1993 and finally selected in 1998 after five (5) years (1994 to 1998) of experimentation. 'Arirang' is a cut flower with bright white color petals and purple core which exhibited early flowering and vigor without stem bending. These can be cultivated in areas or regions with strong winds during summer. 'Arirang' has a very firm growth form and sturdy branches which do not bend when planted in the open fields. Likewise, the plant had high tolerance to flooding and resistance hence could grow vigorously in open areas. It is a fast- growing plant which exhibits high corm production rate. 'Arirang' had a 10.5 cm wide flower and 140 cm plant height. During summer, the average number of days to flowering was recorded at 75 days of 'Arirang'. Top dressing must be conducted once or twice during corm production to enhance corm quality and quantity.

Effect of Controlled Release Fertilizer on the Growth and Flowering of Oncidium 'Sweet Sugar' (완효성비료 시비가 온시디움 생육과 개화에 미치는 영향)

  • Kim, Si Dong;Lee, Hee Doo;Kim, Ju Hyoung;Kim, Tae Jung
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.4
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    • pp.246-250
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    • 2009
  • This study was conducted to determine the effect of controlled-release fertilizer on growth of Oncidium. Leaf and pseudobulb length increased with controlled-release fertilizer from 2 g to 3 g treatment compared to hyponex and controlled-release fertilizer I g treatment, while leaf width and number of leaf was not significant among treatments. Plant weight increased with controlled-release fertilizer from 2 g to 3 g treatment compared to hyponex and controlled-release fertilizer I g treatment. Flowering date(bloomed October early) did not show significant difference among treatments. The number of flowers showed the most in controlled-release fertilizer 3 g treatment as 62.4 ea/plant, but was not different significantly among treatments. Flower stem length and width were also non-significant among treatments. Branching numbers increased in the controlled-release fertilizer 2 g and 3 g treatments. Mineral elements of shoot increased from 2 g to 3 g treatment rather than the controlled-release fertilizer 1g treatment. Especially, K content was higher compared to N and P content. Therefore, appropriate controlled-release fertilizer amount for Oncidium was recommended as 2 g.

Powering Performance Characteristics of 2,5000 Ton Class Trimaran (2,5000톤급 삼동선의 저항추진특성)

  • Kuk-Jin Kang;Chun-Ju Lee;Do-Hyun Kim
    • Journal of the Society of Naval Architects of Korea
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    • v.38 no.3
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    • pp.14-22
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    • 2001
  • This paper presents the results of hull form design and powering performance for a 2.500 ton class trimaran frigate. A series of resistance tests and numerical calculations have been conducted to figure out the influences of side-hull form and position on the resistance characteristics of the trimaran. And the propulsion test was conducted to investigate the propulsion efficiency of the trimaran, and the powering performance of the trimaran was compared with that of the similar mono-hull ships in full scale. From the research it was found that the longitudinal position of side-hull had a large influence on the resistance characteristics of the trimaran while the side-hull form and transverse position had a small influence on it. The trimaran showed favorable resistance performance when the side-hull was located near the stern of trimaran, which was resulted from the fact that the side-hull stem was located near the primary wave hollow generated by the main hul1. The powering performance of the trimaran was superior to that of the similar mono-hull ships above the middle speed range.

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Antioxidant and Antimicrobial Activities of The Extracts from Native Camellia japonica in Korea (국내 자생 동백나무(Camellia japonica L.) 추출물의 항산화 및 항미생물 활성)

  • 이숙영;김선민;황은주;표병식
    • Korean Journal of Plant Resources
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    • v.17 no.3
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    • pp.314-322
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    • 2004
  • This research has been undertaken to increase availiability of native Camellia japonica leaf and flower in Korea as a edible-medicinal resource. Chemical compositions, antioxidant and antimicrobial activities in different parts of camellia were investigated. Crude protein contained the highest in young leaves(14.22%) but less than 10% in different parts. The contents of crude fat and crude ash were 60.48% in seeds and 5.16% in mature leaves, respectively. Methanol extract of young leaf, flower, and flower bud in camellia showed strong antioxidant activity compared with different parts. Also, antioxidant activity of these was higher than that of BHT, but weaker than that of VtC. Antioxidant activity of various parts in camellia were in order of young leaf > flower bud > flower > mature leaf > stem > bark. Extracts of mature leaf and bark had a remarkable antibacterial activity(0 CFU/$m\ell$) on Bacillus subtilis. The young leaf extract displayed effective growth inhibition against B. subtilis, Candida albicans, and Trichosporon beigelii.

The increasing hematopoietic effect of the combined treatment of Korean Red Ginseng and Colla corii asini on cyclophosphamide-induced immunosuppression in mice

  • Lee, Yuan Yee;Irfan, Muhammad;Quah, Yixian;Saba, Evelyn;Kim, Sung-Dae;Park, Seung-Chun;Jeong, Myung-Gyun;Kwak, Yi-Seong;Rhee, Man Hee
    • Journal of Ginseng Research
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    • v.45 no.5
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    • pp.591-598
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    • 2021
  • Background: Hematopoiesis is the production of blood cells from hematopoietic stem cells (HSCs) that reside in the bone marrow. Cyclophosphamide (CTX) is a chemotherapy drug that suppresses the immune system. Korean Red Ginseng (KRG) and Colla corii asini (CCA) have been traditionally used for boosting the immune system. Methods: HSCs in the bone marrow, and immune cell subtype in splenocytes, PBMCs, and thymocytes were investigated. Serum levels of hematopoietic-related markers were analyzed using ELISA. Protein expression in spleen tissue was analyzed using western blot analysis. Hematoxylin & eosin staining in the femurs of mice were also conducted. Results: The combination of KRG and CCA with a ratio of 3:2 increased HSCs, CD3 and CD8+ T cells in the circulation, and CD3 T cells in the spleen. A ratio of 2:3 (KRG:CCA) increased the thymic regulatory T cells and recovered the CD3 T cells in the spleen and circulation while recovering proteins in the JAK-STAT pathway in the spleen. Overall, blood cell population and differentiating factors vital for cell differentiation were also significantly recovered by all combinations especially in ratios of 3:2 and 2:3. Conclusion: A ratio of 3:2 (KRG:CCA) is the most ideal combination as it recovered the HSC population in the bone marrow of mice.