Browse > Article
http://dx.doi.org/10.1016/j.jgr.2021.02.004

The increasing hematopoietic effect of the combined treatment of Korean Red Ginseng and Colla corii asini on cyclophosphamide-induced immunosuppression in mice  

Lee, Yuan Yee (College of Veterinary Medicine, Kyungpook National University)
Irfan, Muhammad (College of Veterinary Medicine, Kyungpook National University)
Quah, Yixian (College of Veterinary Medicine, Kyungpook National University)
Saba, Evelyn (Department of Veterinary Biomedical Sciences, Faculty of Veterinary and Animal Sciences, Pir Mehr Ali Shah Arid Agriculture University)
Kim, Sung-Dae (College of Veterinary Medicine, Kyungpook National University)
Park, Seung-Chun (College of Veterinary Medicine, Kyungpook National University)
Jeong, Myung-Gyun (R&D Headquarters, Korea Ginseng Cooperation)
Kwak, Yi-Seong (R&D Headquarters, Korea Ginseng Cooperation)
Rhee, Man Hee (College of Veterinary Medicine, Kyungpook National University)
Publication Information
Journal of Ginseng Research / v.45, no.5, 2021 , pp. 591-598 More about this Journal
Abstract
Background: Hematopoiesis is the production of blood cells from hematopoietic stem cells (HSCs) that reside in the bone marrow. Cyclophosphamide (CTX) is a chemotherapy drug that suppresses the immune system. Korean Red Ginseng (KRG) and Colla corii asini (CCA) have been traditionally used for boosting the immune system. Methods: HSCs in the bone marrow, and immune cell subtype in splenocytes, PBMCs, and thymocytes were investigated. Serum levels of hematopoietic-related markers were analyzed using ELISA. Protein expression in spleen tissue was analyzed using western blot analysis. Hematoxylin & eosin staining in the femurs of mice were also conducted. Results: The combination of KRG and CCA with a ratio of 3:2 increased HSCs, CD3 and CD8+ T cells in the circulation, and CD3 T cells in the spleen. A ratio of 2:3 (KRG:CCA) increased the thymic regulatory T cells and recovered the CD3 T cells in the spleen and circulation while recovering proteins in the JAK-STAT pathway in the spleen. Overall, blood cell population and differentiating factors vital for cell differentiation were also significantly recovered by all combinations especially in ratios of 3:2 and 2:3. Conclusion: A ratio of 3:2 (KRG:CCA) is the most ideal combination as it recovered the HSC population in the bone marrow of mice.
Keywords
Colla corii asini; cyclophosphamide; hematopoiesis; Panax ginseng; immunosuppression;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Avalos BR, Lazaryan A, Copelan EA. Can G-CSF cause leukemia in hematopoietic stem cell donors? Biol Blood Marrow Transplant 2011;17(12):1739-46.   DOI
2 Kisielow P. How does the immune system learn to distinguish between good and evil? The first definitive studies of T cell central tolerance and positive selection. Immunogenetics 2019;71:513-8.   DOI
3 Ansel KM, Djuretic I, Tanasa B, Rao A. Regulation of Th2 differentiation and Il4 locus accessibility. Annu Rev Immunol 2006;24:607-56.   DOI
4 Orkin SH, Zon LI. Hematopoiesis: an evolving paradigm for stem cell biology. Cell 2008;132(4):631-44.   DOI
5 Ahlmann M, Hempel G. The effect of cyclophosphamide on the immune system: implications for clinical cancer therapy. Cancer Chemother Pharmacol 2016;78(4):661-71.   DOI
6 Qiu J. Traditional medicine: a culture in the balance. Nature 2007;448(7150):126-8.   DOI
7 Ratan ZA, Youn SH, Kwak Y-S, Han C-K, Haidere MF, Kim JK, et al. Adaptogenic effects of Panax ginseng on modulation of immune functions. J Ginseng Res 2021;45(1):32-40.   DOI
8 Li Y, He H, Yang L, Li X, Li D, Luo S. Therapeutic effect of Colla corii asini on improving anemia and hemoglobin compositions in pregnant women with thalassemia. Int J Hematol 2016;104(5):559-65.   DOI
9 Luo X, Li S. Compendium of materia medica: bencao gangmu. Beijing: Foreign Languages Press; 2003.
10 Groux H, Powrie F. Regulatory T cells and inflammatory bowel disease. Immunol Today 1999;20(10):442-5.   DOI
11 Socolovsky M, Lodish HF, Daley GQ. Control of hematopoietic differentiation: lack of specificity in signaling by cytokine receptors. Proc Natl Acad Sci 1998;95(12):6573-5.   DOI
12 Hyun SH, Ahn H-Y, Kim H-J, Kim SW, So S-H, In G, et al. Immuno-enhancement effects of Korean Red Ginseng in healthy adults: a randomized, double-blind, placebo-controlled trial. J Ginseng Res 2021;45(1):191-8.   DOI
13 Chang S, Collins PL, Aune TM. T-bet dependent removal of Sin3A-histone deacetylase complexes at the Ifng locus drives Th1 differentiation. J Immunol 2008;181(12):8372-81.   DOI
14 Wang Y, Meng Q, Qiao H, Jiang H, Sun X. Role of the spleen in cyclophosphamide-induced hematosuppression and extramedullary hematopoiesis in mice. Arch Med Res 2009;40(4):249e55.   DOI
15 Shi Y, Liu CH, Roberts AI, Das J, Xu G, Ren G, et al. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and T-cell responses: what we do and don't know. Cell Res 2006;16(2):126-33.   DOI
16 Oda A, Tezuka T, Ueno Y, Hosoda S, Amemiya Y, Notsu C, Kasahara T, Nishiyama C, Goitsuka R. Niche-induced extramedullary hematopoiesis in the spleen is regulated by the transcription factor Tlx1. Sci Rep 2018;8(1):8308.   DOI
17 Rawlings JS, Rosler KM, Harrison DA. The JAK/STAT signaling pathway. J Cell Sci 2004;117(8):1281-3.   DOI
18 Seif F, Khoshmirsafa M, Aazami H, Mohsenzadegan M, Sedighi G, Bahar M. The role of JAK-STAT signaling pathway and its regulators in the fate of T helper cells. Cell Commun Signal 2017;15(1):23.   DOI
19 Herrington J, Smit LS, Schwartz J, Carter-Su C. The role of STAT proteins in growth hormone signaling. Oncogene 2000;19(21):2585-97.   DOI
20 Kortylewski M, Kujawski M, Wang T, Wei S, Zhang S, Pilon-Thomas S, Niu G, Kay H, Mule J, Kerr WG, et al. Inhibiting Stat3 signaling in the hematopoietic system elicits multicomponent antitumor immunity. Nat Med 2005;11(12):1314-21.   DOI
21 Passegue E, Jamieson CH, Ailles LE, Weissman IL. Normal and leukemic he- matopoiesis: are leukemias a stem cell disorder or a reacquisition of stem cell characteristics? Proc Natl Acad Sci 2003;100(suppl 1):11842-9.   DOI
22 Welte T, Zhang SS, Wang T, Zhang Z, Hesslein DG, Yin Z, Kano A, Iwamoto Y, Li E, Craft JE, et al. STAT3 deletion during hematopoiesis causes Crohn's disease-like pathogenesis and lethality: a critical role of STAT3 in innate immunity. Proc Natl Acad Sci 2003;100(4):1879-84.   DOI
23 Bronte V, Pittet MJ. The spleen in local and systemic regulation of immunity. Immunity 2013;39:806-18.   DOI
24 Morris R, Kershaw NJ, Babon JJ. The molecular details of cytokine signaling via the JAK/STAT pathway. Protein Sci 2018;27:1984-2004.   DOI
25 Lynch N, Berry D. Differences in perceived risks and benefits of herbal, over-the-counter conventional, and prescribed conventional, medicines, and the implications of this for the safe and effective use of herbal products. Complement Ther Medicine 2007;15(2):84-91.   DOI
26 Wu H, Ren C, Yang F, Qin Y, Zhang Y, Liu J. Extraction and identification of collagen-derived peptides with hematopoietic activity from Colla Corii Asini. J Ethnopharmacol 2016;182:129-36.   DOI
27 Freund M, Link H, Welte K. Cytokines in hemopoiesis, oncology, and AIDS. Heidelberg: Springer-Verlag; 2012.
28 Cheng H, Zheng Z, Cheng T. New paradigms on hematopoietic stem cell differentiation. Protein Cell 2020;11(1):34-44.   DOI
29 Shao L, Wang Y, Chang J, Luo Y, Meng A, Zhou D. Hematopoietic stem cell senescence and cancer therapy-induced long-term bone marrow injury. Trans Cancer Res 2013;2(5):397-411.   DOI
30 Thomas J, Liu F, Link DC. Mechanisms of mobilization of hematopoietic progenitors with granulocyte colony-stimulating factor. Curr Opin Hematol 2002;9(3):183-9.   DOI