• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2006년도 The 6th Intermational Symposium on Developmental Biotechnology
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• pp.9-9
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• 2006

• International Journal of Stem Cells
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• 제17권2호
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• pp.99-101
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• 2024

• Biomolecules & Therapeutics
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• 제24권4호
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• pp.363-370
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• 2016

Cardiovascular disease is the most common cause of death in diabetic patients. Hyperglycemia is the primary characteristic of diabetes and is associated with many complications. The role of hyperglycemia in the dysfunction of human cardiac progenitor cells that can regenerate damaged cardiac tissue has been investigated, but the exact mechanism underlying this association is not clear. Thus, we examined whether hyperglycemia could regulate mitochondrial dynamics and lead to cardiac progenitor cell dysfunction, and whether blocking glucose uptake could rescue this dysfunction. High glucose in cardiac progenitor cells results in reduced cell viability and decreased expression of cell cycle-related molecules, including CDK2 and cyclin E. A tube formation assay revealed that hyperglycemia led to a significant decrease in the tube-forming ability of cardiac progenitor cells. Fluorescent labeling of cardiac progenitor cell mitochondria revealed that hyperglycemia alters mitochondrial dynamics and increases expression of fission-related proteins, including Fis1 and Drp1. Moreover, we showed that specific blockage of GLUT1 improved cell viability, tube formation, and regulation of mitochondrial dynamics in cardiac progenitor cells. To our knowledge, this study is the first to demonstrate that high glucose leads to cardiac progenitor cell dysfunction through an increase in mitochondrial fission, and that a GLUT1 blocker can rescue cardiac progenitor cell dysfunction and downregulation of mitochondrial fission. Combined therapy with cardiac progenitor cells and a GLUT1 blocker may provide a novel strategy for cardiac progenitor cell therapy in cardiovascular disease patients with diabetes.

• Molecules and Cells
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• 제37권6호
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• pp.473-479
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• 2014

Spermatogonial stem cells (SSCs, also called germline stem cells) are self-renewing unipotent stem cells that produce differentiating germ cells in the testis. SSCs can be isolated from the testis and cultured in vitro for long-term periods in the presence of feeder cells (often mouse embryonic fibroblasts). However, the maintenance of SSC feeder culture systems is tedious because preparation of feeder cells is needed at each subculture. In this study, we developed a Matrigel-based feeder-free culture system for long-term propagation of SSCs. Although several in vitro SSC culture systems without feeder cells have been previously described, our Matrigel-based feeder-free culture system is time- and cost-effective, and preserves self-renewability of SSCs. In addition, the growth rate of SSCs cultured using our newly developed system is equivalent to that in feeder cultures. We confirmed that the feeder-free cultured SSCs expressed germ cell markers both at the mRNA and protein levels. Furthermore, the functionality of feeder-free cultured SSCs was confirmed by their transplantation into germ cell-depleted mice. These results suggest that our newly developed feeder-free culture system provides a simple approach to maintaining SSCs in vitro and studying the basic biology of SSCs, including determination of their fate.

• 농업생명과학연구
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• 제46권6호
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• pp.43-49
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• 2012

본 연구는 수간곡선식을 이용하여 아까시나무의 입목 수간재적표를 개발하고자 수행하였다. 이용한 수간곡선식은 Kozak 모형이었으며, 모형의 모수를 추정하였다. 추정한 모형은 통계적으로 적합성이 인정되었으며, 잔차분석 결과도 양호한 것으로 판정되었다. 따라서 본 모형이 아까시나무 수간재적표를 조제함에 있어 충분히 이용될 수 있을 것으로 사료된다. 그리고 수간재적표는 이용자의 편의를 위하여 수피 포함 및 수피제외 재적표를 조제하고자, 수피두께추정식을 개발하여 이용하였다. 수피두께추정식 역시 통계적으로 유의성이 인정되었다. 따라서, 본 모형이 아까시나무 수간재적표를 조제함에 있어 충분히 이용될 수 있을 것으로 사료된다. 또한, 국내에서 처음으로 개발한 아까시나무의 수간재적표는 임학뿐 만 아니라 타 분야에 널리 사용되기를 기대한다.

• Journal of Periodontal and Implant Science
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• 제52권6호
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• pp.437-454
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• 2022

Embryonic stem cells have been a popular research topic in regenerative medicine owing to their pluripotency and applicability. However, due to the difficulty in harvesting them and their low yield efficiency, advanced cell reprogramming technology has been introduced as an alternative. Dental stem cells have entered the spotlight due to their regenerative potential and their ability to be obtained from biological waste generated after dental treatment. Cell reprogramming, a process of reverting mature somatic cells into stem cells, and transdifferentiation, a direct conversion between different cell types without induction of a pluripotent state, have helped overcome the shortcomings of stem cells and raised interest in their regenerative potential. Furthermore, the potential of these cells to return to their original cell types due to their epigenetic memory has reinforced the need to control the epigenetic background for successful management of cellular differentiation. Herein, we discuss all available sources of dental stem cells, the procedures used to obtain these cells, and their ability to differentiate into the desired cells. We also introduce the concepts of cell reprogramming and transdifferentiation in terms of genetics and epigenetics, including DNA methylation, histone modification, and non-coding RNA. Finally, we discuss a novel therapeutic avenue for using dental-derived cells as stem cells, and explain cell reprogramming and transdifferentiation, which are used in regenerative medicine and tissue engineering.

• International Journal of Stem Cells
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• 제16권1호
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• pp.108-116
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• 2023

γδ T cells are a rare and unique prototype of T cells that share properties with natural killer cells in secondary lymphoid organs. Although many studies have revealed the function and importance of adult-derived γδ T cells in cancer biology and regenerative medicine, the low numbers of these cells hamper their application as therapeutic cell sources in the clinic. To solve this problem, pluripotent stem cell-derived γδ T cells are considered alternative cell sources; however, few studies have reported the generation of human pluripotent stem cell-derived γδ T cells. In the present study, we investigated whether lymphoid lineage γδ T cells were successfully generated from human pluripotent stem cells via hemogenic endothelium under defined culture conditions. Our results revealed that pluripotent stem cells successfully generated γδ T cells with an overall increase in transcriptional activity of lymphoid lineage genes and cytolytic factors, indicating the importance of the optimization of culture conditions in generating lymphoid lineage γδ T cells. We uncovered an initial step in differentiating γδ T cells that could be applied to basic and translational investigations in the field of cancer biology. Based on our result, we will develop an appropriate method to purify γδ T cells with functionality and it helpful for the study of basic mechanism of γδ T cells in pathophysiologic condition as well as clinic application.

• The Plant Pathology Journal
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• 제18권1호
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• pp.54-55
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• 2002

Soaked black rot symptom was observed on the stem of Angelica acutiloba from July to August 2000 at Kumsan, Chungnam in Korea. This disease usually occurred under humid and high temperature conditions. The lesions on the stem appeared as soft rot with brown elliptical spots, which developed into large black spots at a later stage. When the bacterial isolates from the diseased plants were inoculated onto healthy plants by artificial needle prick method, symptoms similar to that observed in the fields developed. According to the cultural characteristics and pathogenicity of the isolates on the host plant the causal bacterium was identified as Xanthomonas campestris. This study proposed that the disease be named "bacterial black stem rot of A. acutiloba"loba".

• 대한화장품학회지
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• 제49권2호
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• pp.169-176
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• 2023

본 연구는 알로에 형성층으로부터 유도된 캘러스에서 줄기세포를 추출하고, 이에 대한 항산화 활성 및 피부 탄력 개선효과를 확인하고자 하였다. 알로에 형성층 유래 줄기세포 추출물 AloStem과 캘러스의 세포 생존율을 확인한 결과, AloStem은 98.27%, 캘러스는 71.31%의 세포가 생존하는 것으로 확인되었다. AloStem의 DPPH 항산화 활성을 측정한 결과, 알로에 추출물보다 AloStem에서 2 배 이상의 항산화 효과를 가지는 것으로 확인되었다. AloStem이 함유된 겔에 대한 인간진피섬유아세포의 독성을 확인한 결과, 0.25% 농도까지 영향을 미치지 않았으며, elastin, COL1A1 및 HAS2 mRNA 발현량을 증가시켰다. 또한, AloStem이 함유된 시트마스크를 이용하여 피부탄력 리프팅 효과에 대한 임상적 연구를 성인남녀 21 명을 대상으로 2 주간 진행한 결과, 피부 길이가 시트마스크 사용 전에는 116.75 ± 5.58 mm로 나타났으나, 2 주 사용 후에는 117.44 ± 5.17 mm 로 0.59%가 증가된 것으로 확인되어 AloStem을 함유한 시트마스크는 탄력 개선에 의한 리프팅 효과에 도움을 주는 것으로 최종 확인되었다.

• BMB Reports
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• 제42권2호
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• pp.72-80
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• 2009

In mammals, major progress has recently been made with the dissection of early embryonic cell specification, the isolation of stem cells from early embryos, and the production of embryonic-like stem cells from adult cells. These studies have overcome long-standing species barriers for stem cell isolation, have revealed a deeper than expected similarity of embryo cell types across species, and have led to a better understanding of the lineage identities of embryo-derived stem cells, most notably of mouse and human embryonic stem (ES) cells. Thus, it has now become possible to propose a species-overarching classification of embryo stem cells, which are defined here as pre- to early post-implantation conceptus-derived stem cell types that maintain embryonic lineage identities in vitro. The present article gives an overview of these cells and discusses their relationships with each other and the conceptus. Consequently, it is debated whether further embryo stem cell types await isolation, and the study of the earliest extraembryonically committed stem cells is identified as a promising new research field.