• BMB Reports
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• v.47 no.11
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• pp.643-648
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• 2014

Stanniocalcin (STC), a glycoprotein hormone originally discovered in fish, has been implicated in calcium and phosphate homeostasis. While fishes and mammals possess two STC homologs (STC1 and STC2), the physiological roles of STC2 are largely unknown compared with those of STC1. In this study, we identified Ran-binding protein M (RanBPM) as a novel binding partner of STC2 using yeast two-hybrid screening. The interaction between STC2 and RanBPM was confirmed in mammalian cells by immunoprecipitation. STC2 enhanced the RanBPM-mediated transactivation of liganded androgen receptor (AR), but not thyroid receptor ${\beta}$, glucocorticoid receptor, or estrogen receptor ${\beta}$. We also found that AR interacted with RanBPM in both the absence and presence of testosterone (T). Furthermore, we discovered that STC2 recruits RanBPM/AR complex in T-dependent manner. Taken together, our findings suggest that STC2 is a novel RanBPM-interacting protein that promotes AR transactivation.

• Proceedings of the PSK Conference
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• 2001.04a
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• pp.213.3-214
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• 2001

• Proceedings of the PSK Conference
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• 2002.04a
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• pp.127.1-127.1
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• 2002

• Proceedings of the PSK Conference
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• 2001.10a
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• pp.273.2-273.2
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• 2001

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.4
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• pp.307-313
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• 2010

Stanniocalcin 1 (STC1) is a glycoprotein hormone that is important in the maintenance of calcium and phosphate homeostasis in both fish and mammals. STC1 and its paralog STC2 are expressed in multiple tissues in fishes, although the physiological roles of piscine STCs are still unclear compared with those of mammals. In this study, we cloned olive flounder STC1 (ofSTC1) and ofSTC2 cDNAs into pET28a vector and used E. coli Rosetta (DE3) as the host strain for protein expression. Expression experiments were carried out using isopropyl-$\beta$-D-thiogalactoside (IPTG) and nickel affinity chromatography. We could identify the recombinant proteins as single 29.5 kDa (ofSTC1) and 33.2 kDa (ofSTC2) bands in the insoluble fraction on sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE). These results indicate that ofSTC1 and ofSTC2 were expressed as insoluble proteins in E. coli. Furthermore, the injection of ofSTC1 protein into juvenile tilapia resulted in a decrease of the serum calcium level. These results suggest that the purified fish STC1 and STC2 proteins may be used to elucidate the physiological role of STCs in fishes.

• Proceedings of the PSK Conference
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• 2001.10a
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• pp.246.2-246.2
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• 2001

• Proceedings of the PSK Conference
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• 2002.04a
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• pp.160.1-160.1
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• 2002

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.2
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• pp.217-224
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• 2014

A total of 150 growing ducks were assigned to five dietary treatments to study the effect of sea tangle and charcoal (STC) supplementation on growth performance and meat characteristics in a completely randomized design. There were six replicates and five ducklings in each replication. The five dietary treatments were control, antibiotic, and 0.1%, 0.5%, and 1% STC supplemented diets. No significant differences were found on ADG, ADFI, and gain:feed among treatments in different weeks. The overall (0 to 3 weeks) ADFI decreased in antibiotic treatment (p<0.05) whereas the gain:feed increased significantly upon 1.0% STC supplementation compared to control (p<0.05). No significant variation was found in meat chemical composition except crude fat content which was high in 1.0% STC dietary group (p<0.05). Meat cholesterol was reduced in 0.1% STC group (p<0.05) compared to other dose levels while serum cholesterol was unaffected. High density lipoprotein (HDL) content was high in 1.0% STC (p<0.05) and low density lipoprotein (LDL) was low in 0.1% and 1.0% STC dietary groups (p = 0.06). No significant effect was found on the thiobarbituric acid reactive substances (TBARS) of fresh meat, whereas the TBARS value of meat preserved for 1 week was reduced significantly in STC dietary groups (p<0.05). The 0.1% STC dietary group showed an increased myristic acid (p = 0.07) content whereas, the content of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids increased in STC supplementation than antibiotic group (p<0.05). An increased concentration of omega-3 fatty acids and a reduced ratio of n-6/n-3 PUFA ratio was found upon 1.0% STC supplementation compared to antibiotic dietary group (p<0.05). Therefore, 1.0% STC dietary supplementation can be used as alternatives to antibiotics in duck production.

• Geophysics and Geophysical Exploration
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• v.10 no.4
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• pp.299-307
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• 2007

Slowness time coherence (STC) technique has been applied to 3-receiver slim hole sonic log using 3 NX sized concrete model holes of different physical properties. We analyzed the effects of different source center frequencies on the wave forms, their amplitude spectra, and their STC results. We could determine the sonic velocity of each mode accurately by the application of STC method with the semblance projection and efficient selection of center frequency. Theoretical model and experimental model hole studies indicate that 4-receiver condition is the most ideal for STC in near surface slim hole sonic log. The result also indicates that favorable STC result can be obtained from three-receiver sonic log provided with the help of the first arrival picking method.

• 제어로봇시스템학회:학술대회논문집
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• 1988.10a
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• pp.584-589
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• 1988

Tuning of digital STC parameters for the system with unknown and time varying parameters as well as delays presents many preconditions and difficulties. In order to eliminate these difficulties and to enhance STC control functions, the present study employs the metbod of expert control for STC and to implement it in nod-minimum phase plants. In this paper, implimedtation of the Expert STC for process control, STC. parameters is carried out by tuning systematically and by applying these parameters to non-minimum phase plant. This controller supervises a status of the plant, and robust control function for time delay or change of the plant is demonstrated by computer simulations.