• Asian Pacific Journal of Cancer Prevention
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• v.16 no.10
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• pp.4161-4168
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• 2015

Colorectal cancer (CRC) is a worldwide health problem, being the third most commonly detected cancer in males and the second in females. Rising CRC incidence trends are mainly regarded as a part of the rapid 'Westernization' of life-style and are associated with calorically excessive high-fat/low-fibre diet, consumption of refined products, lack of physical activity, and obesity. Most recent epidemiological and clinical investigations have consistently evidenced a significant relationship between obesity-driven inflammation in particular steps of colorectal cancer development, including initiation, promotion, progression, and metastasis. Inflammation in obesity occurs by several mechanisms. Roles of imbalanced metabolism (MetS), distinct immune cells, cytokines, and other immune mediators have been suggested in the inflammatory processes. Critical mechanisms are accounted to proinflammatory cytokines (e.g. IL-1, IL-6, IL-8) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$). These molecules are secreted by macrophages and are considered as major agents in the transition between acute and chronic inflammation and inflammation-related CRC. The second factor promoting the CRC development in obese individuals is altered adipokine concentrations (leptin and adiponectin). The role of leptin and adiponectin in cancer cell proliferation, invasion, and metastasis is attributable to the activation of several signal transduction pathways (JAK/STAT, mitogen-activated protein kinase (MAPK), phosphatidylinositol 3 kinase (PI3K), mTOR, and 5'AMPK signaling pathways) and multiple dysregulation (COX-2 downregulation, mRNA expression).

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.2
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• pp.937-943
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• 2014

Polymorphisms in miRNA binding sites have been shown to affect miRNA binding to target genes, resulting in differential mRNA and protein expression and susceptibility to common diseases. Our purpose was to predict SNPs (single nucleotide polymorphisms) within miRNA binding sites of inflammatory genes in relation to gastric cancer. A complete list of SNPs in the 3'UTR regions of all inflammatory genes associated with gastric cancer was obtained from Pubmed. miRNA target prediction databases (MirSNP, Targetscan Human 6.2, PolymiRTS 3.0, miRNASNP 2.0, and Patrocles) were used to predict miRNA target sites. There were 99 SNPs with MAF>0.05 within the miRNA binding sites of 41 genes among 72 inflammation-related genes associated with gastric cancer. NF-${\kappa}B$ and JAK-STAT are the two most important signaling pathways. 47 SNPs of 25 genes with 95 miRNAs were predicted. CCL2 and IL1F5 were found to be the shared target genes of hsa-miRNA-624-3p. Bioinformatic methods could identify a set of SNPs within miRNA binding sites of inflammatory genes, and provide data and direction for subsequent functional verification research.

• Journal of Oral Medicine and Pain
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• v.21 no.2
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• pp.265-277
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• 1996

The aim of this study was to investigate the electromyographic(EMG) activity of masticatory and cervical muscles according to chewing pattern in coronal plane during gum chewing. 70 patients with temporomandibular disorders and 30 dental students without any signs and symptoms of the disorders participated in this study. We measured the activity of masseter (MM), anterior temporalis(TA), sternocleidomastoideus(SCM) and trapezius muscle and recorded the chewing patterns using Biopak system synchronously. Chewing pattern was classified into S- or L-pattern by the midline opening path and short or long type by the lateral distance from midline. Obtained data were analyzed with SAS/STAT Program. The obtained results were as follows : 1. Generally, there was tended to be higher activity in the control group than in the patients group. 2. When comparing EMG activity according to preferred side, the muscle activity was tended to higher on the preferred chewing side than on the contralateral side. However, this difference is insignificant statistically 3. In unilateral affected patients, there was no difference in muscle activity between affected chewing side and unaffected chewing side except for the EMG of the temporalis anterior muscle. 4. Despite the varietal in each of the following variables, there mere no differences in EMG activity during gum chewing: chewing pattern in coronal plane and lateral distance of chewing. 5. The activity of SCM in chewing side was higher than that in contralateral side (p<0.001), but there was no difference in trapezius muscle. 6. In all of the control group, there was appeared L-chewing pattern than not involved the midline during preferred side chewing.

• Polymer(Korea)
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• v.27 no.6
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• pp.536-541
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• 2003

Unsaturated poly(3-hydroxyalkanoate) (UPHA) was biosynthesized and the properties of drug delivery using the polymer matrix were investigated. The biosynthesis of UPHA was carried out by pH-stat fed batch fermentation of Pseudomonas oleovorans (ATCC 29347) grown solely with 10-undecenoic acid as a carbon source. The physical and chemical properties of the biosynethesized UPHA were characterized using NMR, FT-IR, GPC and DSC. The drug release experiments were carried out using HPLC with a diffusion cell fur the release amount of ketoprofen as model drug. The effects of crosslinking degree, patch thickness, and enhancer on the drug release were studied. The drug release rate was linearly decreased and consistent with increased crosslinking degree of the polymer matrix. The duration of drug release was enhanced by the Increased patch thickness. The drug release rate was increased with increased amount of propylene gylcol as an enhancer.

• Journal of Microbiology and Biotechnology
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• v.25 no.8
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• pp.1307-1314
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• 2015

Leukemia inhibitory factor (LIF) is a member of the IL-6 cytokine family, having pleiotropic actions such as maintaining stem cell pluripotency and enabling blastocyst implantation. Because the action of LIF is mediated by a ligand-receptor interaction with the LIF receptor (LIF-R), an antagonist for LIF-R has been developed to inhibit LIF-induced signaling. In this study, we present a novel method for the production and purification of an antagonist to human LIF-R (hLA). His-tagged hLA was expressed in E. coli, and simple purification methods without any endopeptidase cleavage were designed. In addition, we determined the optimal temperature conditions for enhancing the production of soluble hLA. Finally, the bioactivity of His-tagged hLA was examined using STAT3 phosphorylation and receptivity of human endometrial ECC-1 cells. Our strategy provides a rapid and efficient method to produce biologically active recombinant hLA.

• Journal of Veterinary Science
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• v.23 no.4
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• pp.60.1-60.6
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• 2022

Background: A definite diagnosis should be made in the bovine practice field, however, it was difficult to perform laboratory analysis immediately. Currently, three types of portable blood gas analyzers are available in Korea. Objectives: This study aimed to evaluate the correlations among these three analyzers. Methods: Seventy-two plasma samples from Holstein-Friesian cows were used for blood gas analysis, and three instruments (EDAN i15 Vet, VETSCAN i-STAT, and EPOC) were operated simultaneously. Moreover, plasma calcium levels were compared between these portable analyzers and blood chemistry device, which is usually used in a laboratory environment. Pearson analysis was performed to confirm the correlation of each parameter produced with the three instruments and blood chemistry analyzer. Results: As results, high correlation was observed in parameters of pH, pO₂, potassium ion, ionized calcium, and glucose (p < 0.001, r > 0.7). In addition, pCO₂ showed a moderate correlation among the three analyzers (p < 0.001, r > 0.5), and there was no correlation among all instruments for sodium ions. There was also a high correlation between ionized calcium from the three portable devices and total calcium from the biochemistry analyzer (p < 0.001, r > 0.9). Conclusions: In conclusion, there was a high correlation between results from the three different blood gas analyzers used in the bovine clinical field in Korea. Thus, a consistent diagnosis can be made even with different equipment if the operator is aware of the strengths and weaknesses of each piece of equipment and operates it properly.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.6
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• pp.75-83
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• 2004

Focusing on native plants that have high possibility of being introduced as rooftop material, this study was conducted to investigate extensive and easy-to-manage rooftop garden and to raise the utilization of native plants by verifying their growing response to artificial substrate soil depth and irrigation period. The study was conducted from March to September in 2002. Plants tested included Chrysanthemum zawadskii, Sedium middendorffianum, Thymus quinquecostatus, Allium senescens, and Dianthus superbus. Regarding soil depth, it was 5 cm and 10 cm. Irrigation period was non-irrigation, 1-week, 2-weeks, and 3- weeks, Its result is as follows; 1. In case of Sedum middendorffianum Maxim, mortality rate was 0% regardless of soil depth and irrigation period making it very suitable material for rooftop garden. 2. In case of Allium senescens L., mortality rate was 0% regardless of soil depth and irrigation period making it very suitable material for rooftop garden. Therefore, Provided that fertilizing is managed well, it is a plant that can be highly utilized.3. In case of Chrysanthemum zawadskii Herb. Subsp. (Nakai) Y. Lee Stat., the growth of top was lower in 10cm than in 5cm and it grew well in 10cm. When utilizing for rooftop garden, it would be desirable to keep minimum viable soil depth at over 10cm. If there is enough rainfall, soil and soil depth seem to have greater effect on growth than irrigation period does. 4. In case of Diauthus superbus L. var. longicalycinus (Maxim) Williams, rooting rate and growth were better in 10cm than in 5cm. Therefore, it is desirable to keep minimum soil depth at over 10cm. 5. In case of Thymus quinquecostatus Celak, the growth of top and flowering were better in 10cm than in 5cm. Therefore, it seems desirable to have minimum viable soil depth to be over 10cm. In conclusion, the most suitable species for rooftop garden are Sedium middendorffianum and Allium senescens in this experiment. However, Chrysanthemum zwadskii, Thymus quinquecostatus, and Dianthus chinensis also can be utilized greatly when irrigation is managed regularly in artificial mixed soil over 10cm.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.4
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• pp.1457-1461
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• 2012

Purpose: Non small cell lung cancer (NSCLC) is the leading worldwide source of cancer-related deaths. Although some drugs targeting EGFR mutations have been developed, most advanced cases are still incurable. New targets for anticancer drugs are demanded. The kringle 1 domain of hepatocellular growth factor alpha chain (HGFK1) is a potent anti-angiogenesis factor. It has also emerged as a potential anticancer factor in hepatocellular carcinoma (HCC). The expression of HGFK1 protein in patients with NSCLC has not been reported to date. Method: Here, we assessed HGFK1 expression by Western blotting in 103 cases with advanced NSCLC to investigate the impact of HGFK1 on survival. Results: Results revealed 33 (30.1%) patients were classified as high expressors, this being significantly associated with less remote metastasis (P = 0.002) but not with lymph node metastasis (P = 0.062). There was also a significant association between HGFK1 expression and tumor size (P = 0.025) as well as clinical stage (P = 0.012). Kaplan-Meier survival analysis showed that both overall survival (OS) and progression free survival (PFS) of patients with HGFK1 expression were longer than those of patients without HGFK1 expression (P = 0.004 and P = 0.001 respectively). HGFK1 reversed gefitinib inhibition in the resistent NSCLC cell line A431/GR but did not inhibit the proliferation of NSCLC cells A431 and A431/GR directly. Reversion of gefitinib inhibition in A431/GR cells by HGFK1 was related to decreased phosphorylation of ERK and STAT5. Conclusions: HGFK1 may be a useful prognostic factor of advanced NSCLC patients and a potential drug for gefitinib resistant patients.

• Biomolecules & Therapeutics
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• v.24 no.4
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• pp.402-409
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• 2016

It has been found that 4-isopropyl-2,6-bis(1-phenylethyl)phenol (KTH-13), a novel compound isolated from Cordyceps bassiana, is able to suppress tumor cell proliferation by inducing apoptosis. To mass-produce this compound, we established a total synthesis method. Using those conditions, we further synthesized various analogs with structural similarity to KTH-13. In this study, we aimed to test their anti-cancer activity by measuring anti-proliferative and pro-apoptotic activities. Of 8 compounds tested, 4-methyl-2,6-bis(1-phenylethyl)phenol (KTH-13-Me) exhibited the strongest anti-proliferative activity toward MDA-MB 231 cells. KTH-13-Me also similarly suppressed the survival of various cancer cell lines, including C6 glioma, HCT-15, and LoVo cells. Treatment of KTH-13-Me induced several apoptotic signs in C6 glioma cells, such as morphological changes, induction of apoptotic bodies, and nuclear fragmentation and chromatin condensation. Concordantly, early-apoptotic cells were also identified by staining with FITC-Annexin V/PI. Moreover, KTH-13-Me highly enhanced the activation of caspase-3 and caspase-9, and decreased the protein level of Bcl-2. In addition, the phosphorylation levels of Src and STAT3 were diminished in KTH-13-Me-treated C6 cells. Therefore, these results suggest that KTH-13-Me can be developed as a novel anti-cancer drug capable of blocking proliferation, inducing apoptosis, and blocking cell survival signaling in cancer cells.

• Journal of Korean Society of Forest Science
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• v.8 no.1
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• pp.3-10
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• 1968

(1) The present work is a study on the tolerance of salt concentration, salt injuries and growth, under varied conditions, of salt concentration of five kind silvicultural species. (2) Pinus thunbergii, which is tolerant of stat, has shown both low fresh leaf's pH and low gravity, while Pinus densiflora, Abies bolophylla and Pinus koraiensis have respectively shown high fresh leaf's pH and high gravity. (3) Pinus thunbergii, which is tolerant otosalt, has shown high water content ind its lead, hbile such speciesras Pinus densiflora an Abies lophylla, which are intolerant of salt have shownlow water content in their leaves. (4) A signifficance has ibeen found, in the nonreatment fresh les's pH and n gravity, among Pinusthunbergiis Pinu rigida, Pinus koraiensis, Pinus densiflora and Abies holophya. (5) The order of thetlolerance of salt among the tested species is as foohws : Pinus thunbergii, P. Korea iensis, Abies holopylla. (6) The signs of salt injuries have been observed at the tops of the trees 2~3 hours after the application of 1.5% salt solution in the case of Abiihs holop hylla which are intolerant of salt, while they have been of served at the same position 4~6 hours after the 1.5% application in the case of Pinus Koraiensis and Pinus thunbergii.