• Title/Summary/Keyword: STARCH

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Amylase activity and characterization of Bacillus subtilis CBD2 isolated from Doenjang (된장으로부터 분리된 Bacillus subtilis CBD2의 생육특성 및 amylase 활성)

  • Yang, Su-Jin;Lee, Dae-Hoon;Park, Hye-Mi;Jung, Hee Kyoung;Park, Chang-Su;Hong, Joo-Heon
    • Food Science and Preservation
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    • v.21 no.2
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    • pp.286-293
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    • 2014
  • In this study, one GRAS strain was screened from doenjang, a traditional Korean fermented food, as a microorganism producing amylase due to the formation of a clear zone on the medium including soluble starch. From the analysis of the gene sequence of 16S ribosomal RNA, the strain was identified as Bacillus subtilis and was therefore named Bacillus subtilis CBD2. When the nutrient broth medium was prepared with 3% NaCl, 5% glucose, and the initial medium pH 7.0, the B. subtilis CBD2 showed maximum growth. Among soluble starch, corn starch, maize amylopectin, and wheat starch, soluble starch was the most effective carbon source in the production of amylase by B. subtilis CBD2. The amylase from B. subtilis CBD2 showed the highest activities at pH 8.0 and $50^{\circ}C$, and corn starch was the most proper substrate for the enzyme activity. When corn starch was used as a substrate, the production of sugars through enzyme activity increased for 24 h, and then the enzyme activity became constant.

Effect of the Starch Content on the Silicate Dispersion and Rheological Properties of Polypropylene/Starch/Silicate Composites (폴리프로필렌/전분/실리케이트 복합체의 실리케이트 분산 및 유변학적특성에 미치는 전분 함량의 영향)

  • Kim, Youn Cheol;Lee, Chang-Young
    • Korean Chemical Engineering Research
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    • v.46 no.1
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    • pp.106-111
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    • 2008
  • Polypropylene (PP)/corn starch master batch (starch-MB)/silicate composites with different corn starch compositions of 10, 20, 30, 40 and 50 were prepared by melt compounding at $200^{\circ}C$, using lab scale Brabender mixer. The content of silicate was fixed at 5 wt%. The composition of starch-MB in composites was confirmed by the existence of hydroxy group and peak intensity in fourier-transform-infrared (FT-IR) spectrum. The thermal properties of the PP/starch-MB/silicate composites were investigated by differential scanning calorimetry (DSC), and thermogravimetric analyzer (TGA). There was no district change in melting temperature, and TGA curve indicates a decrease in degradation temperature with the increase of starch-MB content. The silicate dispersion of the composites was measured by X-ray diffraction (XRD) and transmission electron microscope (TEM). The degree of silicate dispersion in PP/starch-MB/silicate composites depended on the content of starch-MB. There was detectable change in d-spacing and peak intensity of the composite when the content of starch-MB was higher than 20 wt%. The rheological behavior of the composites was explained by both shear thinning effect and elastic property with the starch-MB amount. These effects were remarkable when the content of starch-MB was higher than 20 wt%. These were confirmed by an oscillatory viscometer at $200^{\circ}C$.

Isolation of Soil Bacteria Secreting Raw-Starch-Digesting Enzyme and the Enzyme Production

  • Sung, Nack-Moon;Kim, Keun;Choi, Sung-Ho
    • Journal of Microbiology and Biotechnology
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    • v.3 no.2
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    • pp.99-107
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    • 1993
  • Two strains (No. 26 and 143) of bacteria which secrete both pectinase and raw-starch-digesting amylase simultaneously, were isolated from various domestic soil samples. The two bacteria were identified as Pasteurella ureae judging by their morphological and physiological characteristics. The optimal culture conditions for the production of raw-starch-digesting enzyme by the Pasteurella ureae 26 were using $NH_4NO_3$ as the nitrogen source at $37^{\circ}C$ with the pH of 7.5, and 15 of C/N ratio. Since the enzyme was produced only when raw or soluble starch was used as a carbon source, but not when glucose or other sugars was used, the enzyme was considered to be an inducible enzyme by starch. Thin layer chromatography of the hydrolyzed product of starch by the raw-starch-digesting enzyme of the strain No. 26 showed that glucose, maltose and other oligosaccharides were present in the hydrolyzates, and therefore the enzyme seemed to be ${\alpha}-amylase$. The enzyme had adsorbability onto raw com starch in the pH range of 3 to 9.

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A Study on Starch-acrylic Graft Copolymerization by Emulsion Polymerization (유화중합에 의한 전분-아크릴 그래프트 공중합에 관한 연구)

  • Hwang, Ju-Ho;Ryu, Hoon;Cho, Ur-Ryong
    • Elastomers and Composites
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    • v.43 no.4
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    • pp.221-229
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    • 2008
  • Starch as matrix polymer was used to do graft copolymerization with 2-ethylhexylacrylate, methyl methacrylate and acrylic acid. The polymerization was carried out by radical emulsion polymerization with increasing contents of starch. When 0.174% of $\alpha$-amylase as enzyme for starch was added, it was found that it made the best stable emulsion. The glass transion temperature of the polymerized material was increased with starch contents. The particle size and viscosity of the emulsion increased with starch contents due to the increased hydroxy group. Peel strength also increased with contents of starch because the enhanced hydroxy group caused to increase affinity between substrate surface and polymer materials. However, the initial tackiness decreased with starch contents owing to film hardness by higher glass transion temperature.

Development of Higher Functional Coating Agents for Pulp Mold (II) -Manufacture of mixed coating agents- (펄프몰드용 새로운 고기능 코팅제 제조기술개발(제2보) -혼합코팅제 제조-)

  • 강진하;임현아
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.35 no.1
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    • pp.19-26
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    • 2003
  • In recent years, numerous studies have been carried out to find out the possible substitution of PE-coated paperboards used in packaging of watery or oily foods. Accordingly, this study was carried out to obtain the basic data for producing higher functional coating agents for pulp mold by evaluating various kinds of mixed coating agents. At that time, two kinds of synthetic coating agents(AKD, PYA) and three kinds of natural coating agent(CMC, corn starch, oxidized starch) were used for making the mixed coating agents respectively. Physical properties of coated paperboards were tested. Conclusions obtained from this study were as follows. Based on concentrations, the proper mixture ratios were 10:90(AKD:CMC), 10:90(AKD:corn starch), 10:90(AKD:oxidized starch), 40:60(PVA:CMC), 20:80(PVA:corn starch) and 20:80(PYA:oxidized starch). The mixed coating agent of PYA:corn starch(20:80) was the most efficient coating agent. Consequently, water and oil resistance were improved even with much addition of natural coating agents. We consider that they can be suitable for the packaging used in the storage of higher moisture vegetables and other food, and also can be suitable for oily fried food.

The Effect of Addition of Mungbean Starch and Potato Starch on the Textural and Sensory Characteristics of Peach Pyun (녹두전분과 감자전분 복숭아편의 질감과 관능적 특성)

  • 조재욱
    • The Korean Journal of Food And Nutrition
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    • v.12 no.2
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    • pp.156-163
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    • 1999
  • This study was evaluate of chemical properties. textural charact6eristics and sensory properties prod-uced peach pyuns through the change of gelling agents such as mungbean starch potato starchy and pec-tin and of sugar density with 10%, 20%, and 30%. Sensory evaluation was that peach pyun added mun-gbean starch was more preferable in appearance and texture than peach pyun added potato starch. Peac-h pyun added 0% pectin was more preferable than peach pyun with 1% pectin. As a result of texture analysis the texture of peach pyun added mungbean starch was denser than that added potato starch. Adding 1% pectin to peach pyun increased mechanical properties such as hardness and chewiness. Mun-gbeaan starch pyun with pectin on lightness Potato starch pyun with pectin on redness and potato star-ch pyun with pectin on yellowness were significant(p<001). Lightness has a tendency to decrease with increasing sugar density. Adding 1% pectin has the effect to increase lightness redness and yellowness. The subject parameters that effect overall quality on sensory evaluation were found taste quality tex-ture quality hardness adhesiveness and taste by using regression analysis.

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Influence of Water and Surfactants on Wheat Starch Gelatinization and Retrogradation (수분과 계면활성제가 밀전분의 호화와 노화에 미치는 영향)

  • Shin, Mal-Shick
    • Korean Journal of Food Science and Technology
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    • v.23 no.1
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    • pp.116-121
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    • 1991
  • The effects of water contents and surfactants, sodium stearoyl-lactylate, sucrose ester and distilled monoglyceride(Dimodan) on wheat starch gelatinization and retrogradation were studied by differential scanning calorimetry. The endothermic peak patterns of starch varied with water content of starch. When water content was less than 30%, gelatinization did not occur. The onset temperature of gelatinization peak of native starch was $59{\sim}60^{\circ}C$ and that of endothermal peak of retrograded starch was $50{\sim}55^{\circ}C$. The enthalpy value of retrograded starch were greatest in the $40{\sim}50%$ water content. In the presence of surfactants, gelatinization temperatures shifted slightly to higher temperatures. DSC endothermal enthalpies of the amylose-lipid complex increased. The degree of retrogradation in starch was lower with surfactant than without surfactant, but enthalpy of amylose-lipid complex did not change during storage.

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Physicochemical Properties of Mung Bean Starch Paste, a Main Ingredient of Omija-eui

  • Jang, Keum-Il;Han, Hyun-Jeong;Lee, Kwang-Yeon;Bae, In-Young;Lee, Ji-Yeon;Kim, Mi-Kyung;Lee, Hyeon-Gyu
    • Food Science and Biotechnology
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    • v.18 no.4
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    • pp.991-995
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    • 2009
  • As a principle ingredient in omija-eui, the physicochemical properties of mung bean starch (MBS) paste were investigated and compared to those of rice and corn starch. The amylose and the protein content of MBS were higher than those of rice or corn starch while the total sugar content and the swelling power of MBS were lower. In addition, the clarity of MBS paste was higher than either rice or corn starch paste. Regarding pasting properties, the peak viscosity and cool paste viscosity of MBS were higher than those of either rice or corn starch. During the freeze-thaw cycle, MBS exhibited higher degree of syneresis than corn and rice starch, which decreased with high starch concentration and heating temperature. The paste properties and freeze-thaw stability of MBS showed a potential for improving the quality of omija-eui.

Rheological Characteristics of Germinated Corn Starch (발아 옥수수 전분의 리올로지 특성)

  • Yang, Young-Kook;Lee, Shin-Young;Choi, Kook-Chi
    • Applied Biological Chemistry
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    • v.29 no.4
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    • pp.339-345
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    • 1986
  • Rheological characteristics of starch isolated from germinated corn were investigated and compared with those of nongerminated corn starch. Flow behavior of both starches were Bingham psudoplastic. Consistency index and yield stress of germinated starch solution were lower than those of nongerminated starch solution, while flow behavior index was nearly similar. Concentration dependency of both starch solutions were similar to each other but lower temperature dependency of germinated starch solution was observed. Time dependent characteristics of both starches showed thixotropic behavior, but due to germination, germinated starch showed higher structural decay under shear than nongerminated starch and its elastic properties was weaker.

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Studies on solid inflammable lubricants for refractory slates (내화물 슬레이트용 고체윤활제의 연구)

  • Rho, Seung-Baik;Lee, Haakil;Son, Ki Hun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.3
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    • pp.2308-2313
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    • 2015
  • In order to produce an optimal performance solid lubricant used in the refractory slates, various compositions of starch, graphite and water were evaluated by testing their viscosity, coefficient of friction and wear performance. At 15% starch content, the degree of viscosity increment rose in proportional to graphite content and the lowest coefficient of friction was observed when the graphite content was at 30 wt%. Our results demonstrate that, as the water content decrease, the ratio of solid content increases, which compromises the surface coating resulting in increase of coefficient of friction. The best wear test result was obtained when the starch content was at 15 wt% with graphite content at either 25 wt% or 30 wt%.