• Title/Summary/Keyword: ST25.ST36

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Separation of X-and Y-Bearing Spermatozoa IV. Separtion of bull spermatozoa by the combination of density Gradient Centrifugation and Sephadex Gel filtration (X-정자와 Y-정자의 분류에 관한 연구 IV. Sephadex Gel여과법과 Percoll중층원심분류법의 병용에 의한 우 정자의 분류)

  • 이주영;정길생;김종배
    • Korean Journal of Animal Reproduction
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    • v.12 no.3
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    • pp.141-147
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    • 1988
  • These experiments were carried out to develop new techniques for in vitro separation of x-and Y-bearing spermatozoa. The results obtained in these experiments were summarized as follows: 1. Following centrifugation of discontinuous percoll density gradient, populatin of spermatozoa increased progressively from low to high density. The highest concentration of spermatozoa was observed at the 4th fraction which included 36.6% of spermatozoa. 2. As increasing percoll concentration, the higher motility index was obtained and the highest motility index(74.2) was obtained at the 5th fraction. 3. The percentage of B-body bearing spermatozoa following percoll density gradient centrifugation was decreased from 39.7% to 25.6%. 4. The sperm population following chromatography by sephadex gel and percoll density gradient centrifugation was decreased in 1st, 5th and 6th fractions but the reverse was turn for 2nd, 3rd and 7th fractions, and the highest sperm concentration was observed at the 7th fraction which included 37.4% of spermatozoa. 5. Motility index of spermatozoa was increased from 77.6 to 79.4 after the sephadex gel filtration, however it was decreased at all fractions after percoll density gradient centrifugation. The lowest motility index(33.2) was obtained from the 7th fraction. 6. The rate of B-body bearing spermatozoa was shown the trend to decrease by the sephadex gel filtration and the trend was accelerated by the percoll density gradient centrifugation. The lowest percentage of B-body bearing spermatozoa, 12.0% was obtained from the 5th fraction.

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The Methods to Activate the Consigned Education of Fashion Retailing Companies in the Dept. of Fashion Design in Junior Colleges (패션 유통업체 위탁교육 활성화 방안)

  • 김효은;구정모
    • Journal of the Korea Fashion and Costume Design Association
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    • v.5 no.1
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    • pp.97-107
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    • 2003
  • This study was conducted to provide the basic data to activate the consigned education of fashion retailing company in the department of fashion design in a junior college. 36 consigned education students from fashion retailing company in Daegu were surveyed and interviewed. The result can be summarized as follows: 1. Their motives of study lie in ‘I wanted to study in a junior college’ (55.6%), ‘It would be some help for my job’. ‘My company recommended’ (16.7%), and ‘I wanted to get a higher degree’ (11.1%). 2. Their preferences in terms of major and subjects lie in ‘fashion marketing’ (30.6%), ‘fashion coordination’ (25.0%), and ‘fashion information’ (19.4%). 3. The occupations they wanted to get are ‘a displayer’ (38.9%), and ‘a shop master’ (30.6%), and ‘a merchandiser’ (8.3%). 4. The ratio of theories to practices they want is ‘40:60ㆍ30:70’ (27.8%), ‘50:50’ (22.2%), ‘20:80’ (11.1%) 5. With regard to their adaptability in school, they answered that ‘I'm doing quite well’ (44.4%), ‘I'm doing OK’. ‘It's a little hard to follow’ (22.2%) 6. About teaching methods and contents, they responded that they want ‘more emphasis on practices’ (41.7%), and they commented that ‘teachers are focused on the syllabus procedure rather than students’ practical improvement’ (19.4%), and ‘I feel loaded with too much assignment’ (13.9%) 7. They wanted to be taught by ‘professors’ (77.8%) and ‘professionals in the field’ (19.4%) In conclusion, it is required that both the college and the company need to develop a customized education program focusing on the field training specialized by their cooperation and constant researches. On the part of junior colleges, adjusting their educational directions to the trend of the 21st century, they need to focus on nurturing the consigned students to be highly qualified professionals required by the industry, using hi-tee equipment and facilities through academic and industrial cooperation.

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A THREE DIMENSIONAL FINITE ELEMENT STRESS ANALYSIS ON THE FORCE DISTRIBUTION BY DISTAL EXTENSION PARTIAL DENTURES EMPLOYING ATTACHMENTS (Attachment를 이용한 후방연장 국소의치에 의한 압력분산에 관한 3차원 유한요소분석)

  • Shin, Sang-Wan;Ahn, Won-Jun;Jung, Yeon-Jin;Lee, Young-Soo;Shim, Kwang-Sup;Yoo, Kwang-Hee
    • The Journal of Korean Academy of Prosthodontics
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    • v.36 no.6
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    • pp.878-887
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    • 1998
  • Although attachments have been utilized for a retainer of removable prostheses during several decades, there is little information on the force distribution by the attachments. This study was undertaken to evaluate the stress patterns developed by partial dentures employing rigid attachments on the supporting structures. Four types of the mandibular removable partial dentures were designed depending upon the position of the rigid attachment and the existency of lingual bracing. Under 100N of vertical and 25N of lateral loads on the 1st and 2nd lower molar of partial denture, stress distribution patterns and displacement were analysed with three dimensional finite element method by ANSYS version 5.3. 1. The highest stresses were concentrated on the distal alveolar crest of posterior abutment and the second stresses on the apical region of posterior abutment in the models. 2. The greatest displacement were shown on the distal alveolar crest of posterior abutment and the second displacement on the distal alveolar crest of anterior abutment in the models. 3. There was little difference between the models with intraoral attachment and those with extracoronal attachment. 4. There was little difference between the models with and without the lingual bracing.

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Prevalence and Risk Factors of Dementia in the Community Elderly (지역사회 노인 치매 유병율과 위험인자)

  • Park, Nam-Hee;Lee, Youn-Mee;E, Lu-Rie
    • Research in Community and Public Health Nursing
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    • v.19 no.1
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    • pp.36-45
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    • 2008
  • Purpose: This study was to estimate the prevalence of dementia in order to estimate the associations of dementia with its risk factors in the community elderly. Methods: The multistage random cluster sampling method was used to select the subjects. The response rate was 94.3%. For the 1st stage screening survey, the Korean-version Mini-Mental State Examination (K-MMSE) and the Bathel Index of activities of daily living (ADL) and instrumental activities of daily living (IADL) were used as primary screening tools. At the 2nd stage. diagnoses were confirmed according to the Clinical Dementia Rating Scale (CBR) and Computer Tomogram (CT). Results: Age-sex adjusted prevalence (%) [95% CI] of dementia was 6.25% [4.47-7.83] (male 4.21% [2.40-6.02]; female 8.28% [5.71-10.85]). Four statistically significant risk factors of the dementia were identified: age 70-74 (OR=1.367), age 75-79 (OR=1.712), age 80-84 (OR=2.465), age 85 over (OR=7.363) illiteracy (OR=3.827); unconsciousness after head injury (OR=3.383), and no exercise (OR=2.188). Hosmer and Lemeshow goodness-of-fit index of dementia risk model was E (legit of dementia)= -4.337+$0.312^*Age(70{\sim}74)+0.538^*Age(75{\sim}79)+0.902^*Age(80{\sim}84)+1.996^*Age$(85over)+$1.342^*$Illiteracy+$1.219^*$Unconsciousness after head trauma+$0.783^*$No exercise. We confirmed that the overall prevalence of dementia in adults aged 65 and older was 94.3%. The risk factors of dementia were explained by age, illiteracy unconsciousness after head trauma and no exercise. Conclusion: These data have been used to estimate the incidence of dementia in elderly community population and to manage the possible role of risk factors as predictors of dementia.

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Estimation of Genetic Parameters for Economic Traits in Korean Native Chickens

  • Sang, Byung Don;Kong, Hong Sik;Kim, Hak Kyu;Choi, Chul Hwan;Kim, Si Dong;Cho, Yong Min;Sang, Byung Chan;Lee, Jun Heon;Jeon, Gwang Joo;Lee, Hak Kyo
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.3
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    • pp.319-323
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    • 2006
  • Heritabilities and genetic correlations of economic traits were estimated with a multiple-trait animal model on 5 different strains of Korea Native Chickens (KNC): Red Brown (RB), Yellow Brown (YB), Gray Brown (GB), Black (B) and White (W). The data used for this study were collected from the Daejeon branch of the National Livestock Research Institute and included 11,233 performance records and 12,729 individual animals. DFREML and SAS BASE/STAT packages were used to estimate genetic parameters and descriptive statistics. The estimated heritabilities for strain RB, YB, GB, B and W, respectively, for age at 1st egg were 0.24, 0.27, 0.12, 0.32 and 0.18; for body weight at first egg were 0.39, 0.43, 0.38, 0.52 and 0.57; for body weight at age of 270 days were 0.43, 0.51, 0.30, 0.52 and 0.67; for egg weight at first egg were 0.08, 0.13, 0.07, 0.06 and 0.07; for egg weight at age of 270 days were 0.37, 0.43, 0.22, 0.34 and 0.41; and for number of eggs laid by age of 270 days were 0.24, 0.25, 0.37, 0.36 and 0.30. The genetic and phenotypic correlations were also estimated.

Acupuncture Stimulation at LI11 Suppresses Seizure and Apoptosis in Hippocampi on an Epilepsy Mouse Model (간질 동물 모델을 이용한 곡지(曲池) 및 족삼리(足三里)의 간질발작 및 해마 신경세포 보호 효과 비교 연구)

  • Lee, Jong Boon;Hwang, Kyoung Min;Yoo, Tae-Won;Bae, Chang-Hwan;Kwon, Sunoh;Kim, Seung-Tae
    • Korean Journal of Acupuncture
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    • v.30 no.1
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    • pp.73-80
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    • 2013
  • Objectives : LI11 has been known to suppress epileptic seizure. Using an mouse epilepsy model, we investigated whether acupuncture stimulation at LI11 can suppress kainic acid(KA)-induced epileptic seizure and apoptosis in the mouse hippocampus. Methods : Eight-week-old male C57/BL6 mice(20~25 g) were given acupuncture at LI11 or ST36 once a day for 3 days. After the last acupuncture stimulations, KA(30 mg/kg) was injected intraperitoneally and the degree of seizure was observed for 90 minutes. Twenty-four hours after KA administration, mice were sacrificed and the neural cell death, astrocyte activation and caspase-3 expression in their hippocampi were investigated. Results : Acupuncture stimulation at LI11 suppressed KA-induced epileptic seizure, neuronal cell death, astrocyte activation and caspase-3 expression. Conclusions : Acupuncture stimulation at LI11 decreases the KA-induced epileptic seizure and protects hippocampal cell death via regulating astrocyte activation and caspase-3 expression.

Clinical Study on Closed Thoracotomy (폐쇄식 흉관삽관술에 관한 임상적 고찰)

  • 이종수
    • Journal of Chest Surgery
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    • v.18 no.4
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    • pp.822-834
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    • 1985
  • Reexpansion of the lung is the most desirable method of filling the pleural space whether it`s contents may be, and closed thoracotomy connected to a water-seal drainage remains the basic therapeutic modality in the treatment of the problems of the pleural space. We usually used rubber mushroom tubes, size No. from 16 to 34 Fr., and performed closed thoracotomy after preliminary thoracentesis to determine the exact depending position. Author reviewed 576 cases of closed thoracotomy which were performed in the Department of Thoracic and Cardiovascular Surgery, Pusan National University Hospital, since Jan., 1980 to June, 1984. The results were as follows: 1. The age distribution was 10 days to 76 years old and mean age of the patients was 36.6 year, and the most prevalent age group was twenties, and sex predisposition was male dominant, 86.9%. 2. The most common etiologic disease group was pneumothorax and the most common etiologic disease was traumatic hemothorax. 3. Sites of tubing were predominantly at posterior axillary line, 7th intercostal space and midclavicular line, 2nd intercostal space even though frequent presence of free pleural space. The two sides, right and left difference of occurrence rate was more frequent at right side, 51.2%. 4. Usually the durations of tubing was less than 10 days, 52.6%, and the number of tubes used to the same patient concomitantly was one, 73.9%, and the time of tubing to the same patient was 1st, 83.6%. 5. The common symptom and sign were dyspnea, 50.0%, chest pain, 30.7%, cough, 10.7%, fever, 6.5%. Especially, fever and cough was the most common symptom and sign in pyogenic empyema, 59.3%. 6. The common etiologic lesions of pneumothorax were blebs and bullae, 73.3%, and of pyogenic empyema was pneumonia, 69.0%. 7. The complication rate of closed thoracotomy was 26.0%. Among these complications, infection was 44.7%, and intercostal neuralgia was 25.3%. 8. 70.9% of all patients recovered with only closed thoracotomy and the rest of patient needed additional some necessary managements such as open thoracotomy [Blebectomy, Resection, Pleurodesis, Decortication, Bleeding control], open drainage, thoracoplasty and so on to have successful results.

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Replacement of the Xenograft Cardiac Valves (이종 조직판막의 재치환수술)

  • 김종환
    • Journal of Chest Surgery
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    • v.21 no.4
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    • pp.619-629
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    • 1988
  • The increasing number of replacement of the substitute cardiac valves were seen in these 2 years. Out of a total 1,408 patients with cardiac valve replacement, 54 required replacement of the substitute valves. Fifty-nine substitute valves replaced were 43 in mitral, 14 in aortic and 2 in tricuspid positions; and they were 36 Ionescu-Shiley, 15 Hancock and 3 Angell-Shiley bioprosthetic valves and 3 St. Jude Medical and 2 Bjork-Shiley prosthetic valves. Primary tissue failure was the most frequent reason of replacement[38 patients] followed by paravalvular leak[9 patients], prosthetic valve endocarditis[6 patients] and valve thrombosis[1 patient] in order. The most pronounced pathology of the failed xenograft valves seen in the primary tissue failure group was calcification and fixation of the cusps with or without tear and defect of the cuspal tissue. The operative mortality rate was 7.4%. Fifty early survivors were followed up for a total of 82.6 patient-years and there was no late death. Actuarial survival rate was 92.3*3.8% at 6 years after surgery. Although the definite tendency toward early and accelerated degeneration of the xenograft valves has been seen in patients younger than 20 to 25 years of age, no strict age limit from where the tissue failure slows down could be determined. The requirement of the ideal substitute valves would be the durability of the recently developed mechanical prostheses armed with the low thrombogenicity of the bioprostheses. At the present time, the need of compromise in selection between less thrombogenic bioprosthetic and more durable mechanical valves should be stressed. The difficulty in choice is yet important in patients of middle age and children where the use of homograft valves may be one of the solution despite of certain limitations from sociomedical reasons.

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Assessment of inter- and intra-fractional volume of bladder and body contour by mega-voltage computed tomography in helical tomotherapy for pelvic malignancy

  • Kim, Sunghyun;You, Sei Hwan;Eum, Young Ju
    • Radiation Oncology Journal
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    • v.36 no.3
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    • pp.235-240
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    • 2018
  • Purpose: We describe the daily bladder volume change observed by mega-voltage computed tomography (MVCT) during pelvic radiotherapy with potential predictors of increased bladder volume variations. Materials and Methods: For 41 patients who received pelvic area irradiation, the volumes of bladder and pelvic body contour were measured twice a day with pre- and post-irradiation MVCT from the 1st to the 10th fraction. The median prescription dose was 20 Gy (range, 18 to 30 Gy) up to a 10th fraction. The upper and lower margin of MVCT scanning was consistent during the daily treatments. The median age was 69 years (range, 33 to 86 years) and 10 patients (24.4%) were treated postoperatively. Results: Overall bladder volume on planning computed tomography was 139.7 ± 92.8 mL. Generally, post-irradiation bladder volume (POSTBV) was larger than pre-irradiation bladder volume (PREBV) (p < 0.001). The mean PREBV and POSTBV was reduced after 10 fraction treatments by 21.3% (p = 0.028) and 25.4% (p = 0.007), respectively. The MVCT-scanned body contour volumes had a tendency to decrease as the treatment sessions progressed (p = 0.043 at the 8th fraction and p = 0.044 at the 10th fraction). There was a statistically significant correlation between bladder filling time and PREBV (p = 0.001). Conclusion: Daily MVCT-based bladder volume assessment was feasible both intra- and inter-fractionally.

The study for Six Sigma Robust Design of Column part for LCD Transfer system (LCD이송장치 Column부의 식스 시그마 강건설계를 위한 연구)

  • Jung D.W.;Chung W.J.;Song T.J.;Bang D.J.;Yoon Y.M.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2005.10a
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    • pp.869-872
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    • 2005
  • This research studied robust design of column part for LCD transfer system. $1^{st}$ DOE(Design of Experiment)was conducted to find out main effect factors. 36 experiments were performed and their results were shows that the geometric parameters(Low-length, Side-length, Upper-thickness, Middle-thickness)are more important than other factors. The main effect plots shows that the maximum deflection of column is minimized with increasing Low-length, Side-length, under-thickness and Middle-thickness. $2^{nd}$ DOE was conducted to obtain RMS(Response Surface Method)equation 25 experiments were conducted. The CCD(Central Composite Design)technique with four factors were used. The coefficient of determination $(R^2)$ for the calculated RSM equation was 0.986. Optimum design was conducted using the RSM equation Multi-island genetic algorithm was used to optimum design. Optimum value for Low-length. Side-length, Upper-thickness and Middle-thickness were 299.8mm, 180.3mm, 21.7mm, 21.9mm respectively. An approximate value of 5.054mm in deflection was expected to be a maximum under the optimum conditions. Six sigma robust design was conducted to find out guideline for control range of design parameter. To acquire six sigma level reliability, the standard deviation of design parameter should be controlled within 2% of average design value.

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