• Journal of Pharmacopuncture
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• v.15 no.2
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• pp.20-23
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• 2012

Panax ginseng is a well-known herbal medicine in traditional Asian medicine, and wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention. However, little has actually been reported on the difference between wild ginseng and cultivated ginseng. Thus, to identify and analyze those differences, we used suppressive subtraction hybridization (SSH) sequences with microarrays, realtime polymerase chain reaction (PCR), and reverse transcription PCRs (RT-PCRs). One of the clones isolated in this research was the chloroplast rpoC1 gene, a ${\beta}$subunit of RNA polymerase. Real-time RT-PCR results showed that the expression of the rpoC1 gene was significantly upregulated in wild ginseng as compared to cultivated ginseng, so, we conclude that the rpoC1 gene may be one of the important markers of wild ginseng.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.34 no.1
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• pp.11-18
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• 2008

Human bone marrow mesenchymal stem cells are thought to be multipotent cells, which are present in adult marrow, that can replicate as undifferentiated cells and that have the potential to differentiate to lineages of mesenchymal tissues, including bone, cartilage, fat, tenden, muscle, and marrow stroma. Cells that have the characteristics of human mesenchymal stem cells could be isolated from marrow aspirates of human and animals. This study was designed to identify and characterize genes specifically expressed by osteogenic supplements -treated cells by suppression subtractive hybridization(SSH) method. The results were as follows: 1. 2 genes were upregulated genes in osteogenic diffeentiation of hMSCs, which is further proved by Northern blot analysis. 2. IGFBP-2 has been identified playing an important role in bone formation. 3. HF1 was also upregulated during osteogenic differentiation, but its role in bone formation is not clear yet.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2017.10a
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• pp.565-568
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• 2017

The security protocols such as SSH and SSL/TLS operate over TCP and encrypt all data from the application layer. However, this method has unnecessary performance degradation because it encrypts even data which does not require confidentiality. In this paper, we propose TCP OENC(TCP Optional Encryption) which optionally encrypts only confidential data by the application user. The proposed TCP OENC is in accordance with TCP standard operation, and it operates if application user demand on encrypting data. Before the TCP OENC sends first encrypted data, performs the key agreement, and then encrypts and sends data which application user is desired by using shared key obtained from the key agreement.

• Food Science and Preservation
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• v.16 no.6
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• pp.824-829
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• 2009

Changes in pH, viable microbial count, chemical freshness, texture, and sensory qualities of Sing Sing Hoe (SSH, fresh-sliced raw fish) were measured over 15 days at $4^{\circ}C$, $-20^{\circ}C$, and $-80^{\circ}C$. The initial pH of SSH was 6.25 at all three storage temperatures, and pH increased slightly after 12 days to pH 6.48 and pH 6.55 at $-20^{\circ}C$ and $-80^{\circ}C$, respectively. The range in viable cell count was 104-106 CFU/g, regardless of storage temperature. The initial content of volatile basic nitrogen (VBN) was 5.8 mg/100 g and became 8.2 mg/100 g or less, and 7.9 mg/100 g or less after 15 days at $-20^{\circ}C$ and $-80^{\circ}C$, respectively. However, pH and VBN values increased significantly after 3 days of storage at $4^{\circ}C$. At this temperature, the K-value was 22.3% after 6 days and 40% or more after 15 days. At $-20^{\circ}C$, the K-value was 9.6% or less after 6 days and 21% or less after 15 days of storage. At $-80^{\circ}C$, the K-value was 8.5% or less after 9 days and 20% or less after 15 days of storage. Compared with the K-value of live fish muscle (10%), freshness similar to that of live fish was maintained for 6 days under both $-20^{\circ}C$ and $-80^{\circ}C$ storage conditions. There was no significant change in texture during storage of SSH at $-20^{\circ}C$ or $-80^{\circ}C$, but SSH stored at $4^{\circ}C$ showed a decrease in texture quality during storage. Sensory scores were high for material stored for up to 3 days at $4^{\circ}C$ and 6 days at $-20^{\circ}C$ or $-80^{\circ}C$. The overall freshness of SSH was maintained for up to 6 days, in comparison with fresh-sliced raw fish, under both frozen storage conditions.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.5
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• pp.744-748
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• 2012

This study was carried out to investigate the incorporation effect of green manure crops (GMC) such as the hairy vetch on improvement of soil environment in reclaimed land during sorghum${\times}$sudangrass hybrid (SSH) cultivation over the past three years from 2009 to 2011. Plots consisted of conventional fertilization (CF) and incorporation of GMC were divided by rates of additional nitrogen fertilizer ($100kg\;ha^{-1}$) and decreased percentage of 30 50 70 100 fertilization in addition to non nitrogen fertilization (NNF). Soil physico-chemical properties, growth and yield potential were examined. The results were as follows. The testing soil was showed strong alkaline saline soil with low organic matter contents and less available phosphate while exchangeable sodium and magnesium were higher. Soil salinity was increased during cultivation of summer crop. However, SSH was not affected by salt content. The fresh weight of GMC at incorporation time was $18,345kg\;ha^{-1}$. Content of total nitrogen at incorporation time was 3.09% and the C/N ratio was 12.8. Fresh and dry matter yield of SSH were higher in the order of 30%, CF, N50%, N70%d, N100%, and NNF. Fresh and dry matter yield of SSH increased in the order of CF ($55,050kg\;ha^{-1}$, $16,250kg\;ha^{-1}$), N contents from 30% to 9%. Soil physical properties, such as bulk density were decrease with incoporation of GMC, while porosity was increased. Soil chemical properties, such as pH was decreased while content of exchangeable calcium, available phosphate, and organic matter were increased. Also contents of exchangeable sodium and potassium were decreased with incorporation of GMC than those before experiment. Thus, we assumed that incorporation of hairy vetch was more effective that can lead to reduce chemical nitrogen fertilizer and to improve soil environment in cultivating SSH on Saemangeum reclaimed land.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.43 no.3 s.345
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• pp.21-32
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• 2006

A new SRAM circuit with row-by-row activation and low-swing write schemes is proposed to reduce switching power of active cells as well as leakage one of sleep cells in this paper. By driving source line of sleep cells by $V_{SSH}$ which is higher than $V_{SS}$, the leakage current can be reduced to 1/100 due to the cooperation of the reverse body-bias. Drain Induced Barrier Lowering (DIBL), and negative $V_{GS}$ effects. Moreover, the bit line leakage which may introduce a fault during the read operation can be eliminated in this new SRAM. Swing voltage on highly capacitive bit lines is reduced to $V_{DD}-to-V_{SSH}$ from the conventional $V_{DD}-to-V_{SS}$ during the write operation, greatly saving the bit line switching power. Combining the row-by-row activation scheme with the low-swing write does not require the additional area penalty. By the SPICE simulation with the Berkeley Predictive Technology Modes, 93% of leakage power and 43% of switching one are estimated to be saved in future leakage-dominant 70-un process. A test chip has been fabricated using $0.35-{\mu}m$ CMOS process to verify the effectiveness and feasibility of the new SRAM, where the switching power is measured to be 30% less than the conventional SRAM when the I/O bit width is only 8. The stored data is confirmed to be retained without loss until the retention voltage is reduced to 1.1V which is mainly due to the metal shield. The switching power will be expected to be more significant with increasing the I/O bit width.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.20 no.3
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• pp.119-130
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• 2015

Impacts of Argo temperature assimilation on the analysis fields in the East Sea is investigated by using DAESROM, the East Sea Regional Ocean Model with a 3-dimensional variational assimilation module (Kim et al., 2009). Namely, we produced analysis fields in 2009, in which temperature profiles, sea surface temperature (SST) and sea surface height (SSH) anomaly were assimilated (Exp. AllDa) and carried out additional experiment by withdrawing Argo temperature data (Exp. NoArgo). When comparing both experimental results using assimilated temperature profiles, Root Mean Square Error (RMSE) of the Exp. AllDa is generally lower than the Exp. NoArgo. In particular, the Argo impacts are large in the subsurface layer, showing the RMSE difference of about $0.5^{\circ}C$. Based on the observations of 14 surface drifters, Argo impacts on the current and temperature fields in the surface layer are investigated. In general, surface currents along the drifter positions are improved in the Exp. AllDa, and large RMSE differences (about 2.0~6.0 cm/s) between both experiments are found in drifters which observed longer period in the southern region where Argo density was high. On the other hand, Argo impacts on the SST fields are negligible, and it is considered that SST assimilation with 1-day interval has dominant effects. Similar to the difference of surface current fields between both experiments, SSH fields also reveal significant difference in the southern East Sea, for example the southwestern Yamato Basin where anticyclonic circulation develops. The comparison of SSH fields implies that SSH assimilation does not correct the SSH difference caused by withdrawing Argo data. Thus Argo assimilation has an important role to reproduce meso-scale circulation features in the East Sea.

• Journal of Life Science
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• v.14 no.4
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• pp.650-656
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• 2004

Oryza grandiglumis (CCDD, 2n=48), one of the wild rice species, has been known to possess fungal-,bacterial-, and insect-resistance against sheath blight, rice blast, bacterial leaf blight and brown plant hopper (Nilaparvata lugens). To rapidly isolate differentially expressed genes responding to fungal and wounding stress, wounding and yeast extract were treated to O. grandiglumis for 24 hrs. Suppression subtractive hybridization (SSH) method was used to obtain differentially expressed genes from yeast extract and wounding treated plants. Seven hundreds and seventy six clones were obtained by subcloning PCR product, and colony array and screening were carried out using radio-isotope labeled cDNA probes prepared from the wounding and yeast extract treated plants. One hundred and fifteen colonies were confirmed as true positive ones. Average insert size of the clones were ranged from 400 bp to 700 bp and all the inserts were sequenced. To decide the identity of those clones, sequences were analyzed by sequence homology via GenBank database. The homology search result showed that 68 clones were matched to the genes with known function; 16 were related to primary metabolism, 5 to plant retrotransposons, 5 to defense related metallothionein-like genes. In addition to that, others were matched to various genes with known function in amino acid synthesis and processing, membrane transport, and signal transduction, so on. In northern blot analysis, induced expressions of ogwfi-161, ogwfi-646, ogwfi-663, and ogwfi-695 by wounding and yeast extract treatments were confirmed. The result indicates that SSH method is very efficient for rapid screening of differentially expressed genes.

• Korean Journal of Remote Sensing
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• v.34 no.6_2
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• pp.1179-1192
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• 2018

In order to detect the Antarctic Polar Front (PF) among the main fronts in the Southern Ocean, this study is based on the combinations of satellite-based sea surface temperature (SST) and height (SSH) observations. For accurate PF detection, we classified the signals as front or non-front grids based on the Bayesian decision theory from daily SST and SSH datasets, and then spatio-temporal synthesis has been performed to remove primary noises and to supplement geographical connectivity of the front grids. In addition, sea ice and coastal masking were employed in order to remove the noise that still remains even after performing the processes and morphology operations. Finally, we selected only the southernmost grids, which can be considered as fronts and determined as the monthly PF by a linear smoothing spline optimization method. The mean positions of PF in this study are very similar to those of the PFs reported by the previous studies, and it is likely to be well represents PF formation along the bottom topography known as one of the major influences of the PF maintenance. The seasonal variation in the positions of PF is high in the Ross Sea sector (${\sim}180^{\circ}W$), and Australia sector ($120^{\circ}E-140^{\circ}E$), and these variations are quite similar to the previous studies. Therefore, it is expected that the detection approach for the PF position applied in this study and the final composite have a value that can be used in related research to be carried out on the long term time-scale.

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2002.11a
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• pp.153-153
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• 2002