• 한국초지조사료학회지
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• 제31권2호
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• pp.143-150
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• 2011

본 연구는 수확시기가 수수${\times}$수수 교잡종 원형 곤포사일리지의 품질에 미치는 영향을 구명하기 위하여 천안에 위치한 국립축산과학원 자원개발부에서 수행되었다. 수수${\times}$수수 교잡종 SS405를 이용하여 숙기별 2회(출수기 및 완숙기)에 걸쳐 수확을 하여 사일리지로 조제하였다. 숙기가 진행됨에 따라 수수${\times}$수수 교잡종원형 곤포사일리지의 조단백질은 증가하는 경향을 보였으나 NDF 및 ADF 함량 그리고 TDN 함량은 비슷한 수준을 유지하였다. 그리고 숙기별 in vitro 건물소화율도 비슷하였다. 숙기별 사일리지의 pH는 3.8~4.4을 유지하였으며, 출수기의 pH는 완숙기의 보다 높았다. 수수 ${\times}$ 수수 교잡종 원형 곤포사일리지의 수확시기가 늦어짐에 따라 젖산 함량은 증가하였으나 초산 함량은 감소하였다(P<0.05). 그리고 출수기의 원형 곤포사일리지의 초산 함량은 트랜치사일리지보다 증가되었다(P<0.05). 완숙기에서는 제조방법에 따라 젖산과 초산 함량은 큰 차이를 보이지 않았다. 이상의 결과를 종합하여 볼 때 양질의 수수 ${\times}$ 수수 교잡종 원형곤포 사일리지 조제를 위해서는 출수기와 완숙기 사이에 수확하여 사일리지를 만드는 것이 사일리지 발효를 향상시킬 수 있다.

• 한국초지조사료학회지
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• 제17권2호
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• pp.167-176
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• 1997

A field experiment was conducted in Chungiu and Jungwon to evaluate growth characteristics, root development, dry matter yield, crude protein yield and palatability according to nitrogen fertilizer level at intercropping cultivation of sorghum $\times$ sudangrass hybrid(hereinafier referred as SSH) and soybean. Nitrogen level was six treatment of Okg, 30kg 60kg, 90kg 120kg, and 150kg per hectare, and cutting date (sorghum $\times$ sudangrass and soybean) was july 28. 1. In the SSH, plant length was increased with increasing nitrogen fertilizer from 0kg/ha to 120kg/ha, but 150kgha treatment was decreased cornpared to 120kg/ha treatment. In the soybean, plant length did not show difference among 0kg, 30kg 60kg, and 90kg per hectare, but 120kg and 150kgfha treatment was on the contrary short. In the SSH, leaf length, leaf width and leaf number were the highest in 120kg/ha treatment, but soybean showed multifarious. 2. Stem diameter of SSH was increased with increasing nitrogen fertilizer. But soybean was thick from 0kg/ha treatment to 60kg/ha treatment, while above 90kg/ha treatment was on the contrary thin. Stem of SSH was hard with increasing nitrogen fertilizer, but soybean was soft with increasing nitrogen fertilizer. Root weight (Dry matter) of SSH was increased with increasing nitrogen fertilizer. On the contrary, soybean was decreased with increasing nitrogen fertilizer. 3. In the dairy cattle and Korean native cattle, palatability according to nitrogen fertilizer was the highest at 90kgha treatment, while 120kgha treatment and 150kgha treatment was the lowest. But in the deer, 30kg/ha treatment of low nitrogen fertilizer was the highest in palatability. 4. Nitrogen content of root of SSH was increased with increasing nitrogen fertilizer(P<0.01). But in soybean, 30kg and 60kg/ha treatment were higher than 0kg, 90kg, 120kg and 150kg/ha treatment(P<0.01). Total niwogen content of soil were high at 90kg 120kg 150kgha trea~ment(P<0.01). 5. Dry matter yield was high at the 90kg 120kg and 150kgha treatment as range from 15, 262kg/ha to 15, 614kg/ha without significant difference among those, but Okgha treatment was the lowest as 11, 183kg/ha (PC 0.05). Protein content of SSH was the highest at 90kg/ha treatment as 8.3 percentage. Soybean was highest at 60kgha treatment as 22.9 percentage. Protein yield was the highest at 90kg/ha treatment as 1, 547.6kg/ha, but Okgha treatment was the lowest as 1, 022.8kg/ha (P<0.01).

• 한국초지조사료학회지
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• 제25권1호
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• pp.7-16
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• 2005

This experiments were carried out to determine growth characteristics, dry matter yield and voluntary intake of silage according to growth stage of Sorghum$\times$sudangrass(SSH) hybrid in mono-cropping and inter-cropping(C; com, T1; cutting of SSH at milk stage. T2; cutting of SSH at dough stage, T3; cutting of SSH at yellow ripe stage, T4; cutting of SSH and soybean at milk stage, T5; cutting of SSH and soybean at dough stage, T6: cutting of SSH and soybean at yellow ripe stage). Results obtained from these experiments are as follows; Plant length and leaf length of SSH(T2, T3, T4. T5 and T6)) were higher than C. T1 treatment was lower than it. C showed $2.3\~2.9$ times higher stem diameter as 29.5mm compare to SSH(T1, T2, T3, T4, T5 and T6). soybean(T4, T5 and T6) was lower in $4.3\~5.4$ times. But SSH of inter-cropping treatment(T4, T5 and T6) showed highly comparing with mono-cropping(T1, T2 and T3) at the same maturity. Leaf rate and stem hardness of SSH(T1, T2, T3, T4, T5 and T6) were lower than C. The fresh yield was high line with T2(72,320kg/ha), T1(69,103kg/ha), T3(68,333kg/ha) and C(57,988kg/ha), dry matter yield was high in line with T3(22.413kg/ha), T2(21,479kg/ha), C(19,252kg/ha) and T6(18,175kg/ha), (P<0.05). Protein dry matter yield was higher in T3(1,434kg/ha), C(1,386kg/ha)T5 and T6(1,345kg/ha) it was lower in T1(872kg/ha), (P<0.05). Crude protein of silage of T4 and T5 was higher than C, T2 and T3 were lower than it(P<0.05), while NDF content was not different. ADF content of T6 was higher than those of the other treatment. The highest hemi-cellulose among treatments was shown in T1 whereas T6 showed the lowest. Fresh intake of silge was 160.4, 155.8, 168.7, 172.9, 132.9, 158.7 and 185.2 g/BW for C, T1, T2, T3, T4, T5 and T6, respectively. Dry matter intake was high in line with T6(60.3g), C(153.8g), T3(53.6g), T5(47.8g), T2(46.8g), T4(35.2g) and T1(34.48g/BW), (P<0.05). Crude protein intake was high in line with T6(3.9g), T5(3.4g), C(3.4g), T2(2.9g), T3(2.9g), T4(2.6g) and T1(2.3g/BW), (P<0.05). As mentioned above the results, mono-cropping(T3) and inter-cropping(T5 and T6) could be recommended as increasing method of sorghum $\times$ sudangrass hybrid silage utilization when silage intake of dry matter and crude protein were considered.

• 한국초지조사료학회지
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• 제17권2호
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• pp.177-186
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• 1997

A field experiment was conducted to evaluate growth characteristics, dry matter yield and crude protein yield according to different planting dates at sorghum $\times$ sudangrass hybrid(SSH) and soybean intercropping. Planting dates were five treatment of may 6(Tl), may 13(T2), may 20(T3), may 27(T4) and june 3(T5), and cutting frequency was two times a year. 1. Plant length of SSH was the highest at T2 as 253cm, but T5 was the shortest as 203cm. In the soybean, T3 and T4 were the highest as 113cm, respectively. Leaf length of SSH was high at T5. In the soybean, T2 was the highest as 17cm. Average leaf width of T2, T3 and T4 was higher than TI and T5. 2. Leaf number of T3(SSH and soybean) was higher than other treatments, Stem diameter of SSH and soybean showed the highest as 12.3mm and 8.6mrn at T5 and T3, respectively. In the SSY mean stem hardness of TI was the highest as 2.5kg/$cm^2$, but soybean was the highest at T1(8.0kg/$cm^2$) 3. Deed stubble according to move seeding date of SSH were 11.4 percentage at TI, and 3.9 percentage at T5 treatment. 4. Total dry matter yield according to move seeding date was the highest at T3 as 20,937kghq but T5 of late seeding was the lowest as 16,04Okgha(P < 0.05). 5. In the first cutting time, protein content of SSH was the highest at T3 as 9.9 percentage, but T1 was the lowest as 8.4 percentage. In the 2nd cutting, T5 was the highest as 8.7% but T1 was the lowest as 6.2%. In the soybean, T5 was the highest as 19.4% but TI of early seeding was the lowest as 16.2 percentage. Crude protein yield was the highest at T3 as 2,233.5kghq but TI of early seeding was the lowest as 1,579.7kgha (P < 0.05). As mentioned above the results, T2(may 13), T3(may 20) and T4(may 27) treatment could be recommended as the best suitable seeding date when drymatter and protein yield were considered.

• BMB Reports
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• 제41권11호
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• pp.796-802
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• 2008

Suppression subtractive hybridization (SSH) cDNA libraries of the giant tiger shrimp, Penaeus monodon, were constructed. In total, 178 and 187 clones from the forward and reverse SSH libraries, respectively, of P. monodon were unidirectionally sequenced. From these, 37.1% and 53.5% Expressed Sequence Tags (ESTs) significantly matched known genes (E-value < 1e-04). Three isoforms of P. monodon progestin membrane receptor component 1: PM-PGMRC1-s (1980 bp), PM-PGMRC1- m (2848 bp), and PM-PGMRC1-l (2971 bp), with an identical ORF of 573 bp corresponding to a deduced polypeptide of 190 amino acids, were successfully identified by RACE-PCR. Interestingly, PMPGMRC1 showed a greater expression level in testes of juvenile than broodstock P. monodon (P < 0.05). Dopamine administration ($10^{-6}$ mol/shrimp) resulted in up-regulation of PM-PGMRC1 in testes of juveniles at 3 hrs post treatment (P < 0.05), but had no effect on PM-Dmc1 (P > 0.05).

• Molecular & Cellular Toxicology
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• 제2권1호
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• pp.60-66
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• 2006

Methylmercury (MeHg), one of the heavy metal compounds, can cause severe damage to the central nervous system in humans. Many reports have shown that MeHg is poisonous to human body through contaminated foods and has released into the environment. Despite many studies on the pathogenesis of MeHg-induced central neuropathy, no useful mechanism of toxicity has been established so far. This study, using of suppression subtractive hybridization (SSH) method, was peformed to identify differentially expressed genes by MeHg in SH-SY5Y human neuroblastoma cell line. We prepared to total RNA from SH-SY5Y cells treated with solvent (DMSO) and $6.25\;{\mu}M\;(IC_{50})$ MeHg and performed forward and reverse SSH. Differentially expressed cDNA clones were screened by dot blot, sequenced and confirmed that individual clones indeed represent differentially expressed genes with real time RT-PCR. These sequences were identified by BLAST homology search to known genes or expressed sequence tags (ESTs). Analysis of these sequences may provide an insight into the biological effects of MeHg in the pathogenesis of neurodegenerative disease and a possibility to develop more efficient and exact monitoring system of heavy metals as ubiquitous environmental pollutants.

• 대한한의학방제학회지
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• 제20권2호
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• pp.65-82
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• 2012

Objectives : The aim of this study was verification of the anti-oxidative and anti-inflammatory effects of Odukhwan(ODH) and Sasinhwan(SSH) in mouse macrophage, RAW264.7 cells. Methods : To investigate the anti-oxidative effect and scavenging activities of DPPH radical, superoxide anions, nitric oxide and peroxynitrite were measured. Cytotoxic activity of extract of ODH and SSH on RAW264.7 cells was measured using MTS assay. To proof the reductive activity of intracellular oxidation, DCFH-DA assay was performed. The nitric oxide(NO) production was measured and pro-inflammatory cytokines and $PGE_2$ were measured by ELISA kit. The levels of inducible nitric oxide synthase(iNOS), cyclooxygenase-2(COX-2) and nuclear NF-${\kappa}B$ p65 expression were detected by western blot. Results : After those analyses, we bring to a conclusion as follows. Both herbal formulations scavenged DPPH radical and nitric oxide. But ODH had no scavenging activity of superoxide anions and SSH had low scavenging activity in peroxynitrite. And the results indicated that ODH and SSH inhibited the LPS-induced NO, $PGE_2$ production and iNOS, COX-2 expression accompanied by an attenuation of IL-$1{\beta}$ and IL-6 production in RAW264.7 cells. They also have suppression effects of LPS-induced NF-${\kappa}B$ activation. Conclusions : ODH and SSH have anti-oxidative and anti-inflammatory effects and they may be a part of database for development of new anti-oxidative and anti-inflammatory drugs.

• 정보보호학회논문지
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• 제33권4호
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• pp.661-670
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• 2023

서버리스 컴퓨팅 패러다임의 등장과 함께 클라우드 기술이 발전함에 따라, 서버 관리를 위한 백엔드 인프라의 구조가 온프레미스부터 최신 컨테이너 기반 서버리스 컴퓨팅까지 다변화되고 있다. 그럼에도 불구하고, 서버 관리를 위한 접속방식은 전통적인 SSH 프로토콜에 여전히 의존하고 있으며, 보안성 및 확장성 측면에서의 한계점으로 인해, 사용자의 서버 인프라 관리 편의성 및 업무 생산성을 저하시킨다. 이러한 문제를 해결하기 위해 본 논문에서는 웹쉘을 프록시 기반의 서버 관리 프레임워크에 적용하여 실용성과 보안성을 갖춘 서버 관리체계 설계에 활용하고자 한다. 흔히 해커들이 서버의 취약점을 이용하여 임의 명령을 실행하기 위해 웹쉘을 사용하지만, 본 논문에서는 서버 관리 측면에서의 웹쉘 기술의 활용성에 대해 고찰하고, 웹쉘 기술을 사용한 접속 프록시 기반 서버 관리 프레임워크를 새롭게 제안한다. 또한, 본 논문에서는 실제 구현을 통하여 제안한 프레임워크가 추가적인 오버헤드 없이 표준적인 서버 접속 프로토콜로 사용되어온 SSH의 단점을 보완하고, 현대의 다양화된 컴퓨팅 환경에서도 대규모의 인프라를 효율적으로 운영할 수 있음을 보인다.

• 한국초지조사료학회지
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• 제31권3호
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• pp.295-304
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• 2011

본 연구는 사일리지 수확시기별 조제방법에 따른 수수${\times}$수수 교잡종 사일리지의 품질에 미치는 영향을 구명하기 위하여 천안에 위치한 국립축산과학원 자원개발부에서 수행되었다. 수수${\times}$수수 교잡종 SS405를 이용하여 숙기별 2회(출수기 및 완숙기)에 걸쳐 수확을 하여 사일리지로 조제하였다. 수수${\times}$수수 교잡종 소포장 및 톤백 사일리지는 수확시기가 늦어짐에 따라 조단백질 함량은 증가하는 경향을 보였으나 섬유소인 ADF 및 NDF 함량은 감소하는 경향을 보였다. 그리고 젖산균 첨가에 의해 NDF와 ADF 함량은 영향을 받지 않았다. 수확시기에 따른 수수${\times}$수수 교잡종 사일리지의 TDN 함량 및 출수기의 in vitro 건물소화율은 조제 방법별로 비슷한 수준을 유지하였다. 사일리지 조제방법별 수확시기에 따른 사일리지의 pH는 3.8~4.4을 유지하였으며, 출수기의 pH는 완숙기 보다 높았다(P<0.05). 조제방법별 수수${\times}$수수 교잡종 사일리지는 수확시기가 늦어짐에 따라 젖산 함량은 증가하였으나(P<0.05) 초산 함량은 감소하였다(P<0.05). 출수기 및 완숙기에서 수수${\times}$수수 교잡종 소포장 사일리지의 젖산함량은 톤백 및 원형곤포 사일리지보다 증가하였으나(P<0.05) 초산함량은 감소하였다(P<0.05). 그리고 톤백 사일리지의 젖산 및 초산함량은 원형곤포사일리지와 비슷하였다. 이상의 결과를 종합하여 볼 때 소포장 및 톤백 사일리지 조제 방법은 양질의 수수${\times}$수수 교잡종 사일리지 제조에 적합하였으며 원형곤포와 동등한 사일리지 품질을 유지하였다.

• 한국정보과학회:학술대회논문집
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• 한국정보과학회 2012년도 한국컴퓨터종합학술대회논문집 Vol.39 No.1(B)
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• pp.7-9
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• 2012

슈퍼컴퓨터는 주로 거대 과학문제를 해결하는데 활용되었으나, 최근 산업체의 신제품 및 신기술 개발을 위한 활용이 확대되는 추세이다. 이를 보다 활성화시키기 위해서는 산업체의 연구개발자들이 슈퍼컴퓨터를 활용하여 보다 쉽고 편리하게 시뮬레이션을 수행할 수 있는 환경의 개발이 요구된다. 이를 통해 저비용/고효율의 제품 개발 기반을 확보할 수 있으며, 산업체의 글로벌 경쟁력 향상을 모색할 수 있다. 용접은 선박, 항공기 등의 대형 구조물뿐만 아니라 금속을 사용하는 소형 구조물의 접합을 위해 널리 활용되는 기술이다. K-WeldPredictor는 용접 기술의 80% 정도를 차지하는 아크 용접을 웹 사이트에서 시뮬레이션 할 수 있도록 지원하는 슈퍼컴퓨터 기반의 시뮬레이터이다. 미국 WeldPredictor와의 기술협력을 통해 개발되었으며, 슈퍼컴퓨터의 높은 보안 수준을 준수하기 위해 별도의 웹 서버를 활용하였다. 특히 웹 서버와 슈퍼컴퓨터와의 안전한 데이터 전송을 위해 SSH 로컬 포트 포워딩 기술을 활용하였다.