• 동의생리병리학회지
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• 제16권6호
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• pp.1277-1283
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• 2002

Silsosangami(SSG) is a formula of treaditional korean medicines as an effective biological response modifier for augmenting host homeostasis of body circulation. Little is known of the biological activity of SSG and previous studies have focused mainly on their anti-thrombosis8). There is a growing interest in the pharmacological potential of the SSG due to the recent finding by our group that SSG and each constituent herbs of SSG were able to inhibit NO and prostaglandin E2 (PGE2) synthesis in murine peritoneal macrophages stimulated with bacterial endotoxin. In this paper, the effects of SSG on platelet aggregation and hemolysis in human blood were studied. SSG provoked remarkable inhibiting effect on platelet aggregation, and APTT were sensitive to the presence of this SSG. Using an in vitro system, APTT was delayed with the increment of the concentrations of these seven compounds. These results suggested that SSG might be used as a novel antithrombotic therapeutic agents in post-myocardial infarction. A SSG reduced NO production in mouse peritoneal macrophages stimulated with lipopolysaccharide, without the influence on the activity of iNOS being observed. SSG significantly reduced mouse paw edema induced by carrageenan. Western blot analysis showed that SSG reduced the expression of iNOS. The results indicate that SSG exerts anti-inflammatory effects related to the inhibition of NO production, which could be due to a decreased expression of iNOS.

• 한국정보전자통신기술학회논문지
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• 제4권4호
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• pp.311-317
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• 2011

최근 유비쿼터스 환경에서 바코드 대신 RFID가 대치되었지만, RFID 는 무선 주파수를 사용하기 때문에 프라이버시와 보안성에 많은 문제점을 가지고 있다. 첫째는 비인가 리드가 임의 RFID 태그의 ID 정보를 쉽게 읽어 들일 수 있고, 둘째는 공격자가 수집한 태그 ID 정보를 이용하여 합법적인 리더에게 인가된 태그인 것처럼 쉽게 속일 수 있다. 본 논문에서는 SSG 기반 개선된 RFID 인증 프로토콜을 제안하였다. SSG 알고리즘은 LFSR 과 선택로직만으로 구성되어 있다. 따라서 RFID 태그와 같은 극히 제한적인 자원을 갖는 시스템에서 하드웨어 구현이 가능하며, 출력 비트 스트림은 의사난수로 사용함으로써 다양한 공격에 안전하다. 제안한 프로토콜은 SSG를 기반으로 하기 때문에 안전하고 효율적이다.

• 한국산학기술학회논문지
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• 제17권2호
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• pp.15-20
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• 2016

좁은 면적을 단기간에 개량시킬 수 있는 장점이 있는 그라우팅 공법은 지반의 특성과 지하수위 등에 따라 주입 효과의 차이가 있다. 특히 투수성이 큰 지반에서는 시간 경과에 따라서 용탈현상이 발생하여 강도저하 및 차수 성능이 저하되어 품질에 문제가 생길 수 있다. 최근 이와 같은 문제점을 해결하기 위해 초기강도 확보 및 용탈 방지와 동시에 균열 자기치유 특성을 가지는 SSG가 개발되었다. 이 연구에서는 두 가지 양생조건에 대해 SSG의 일축압축강도 시험을 수행하여 양생기간에 따른 일축압축 강도를 파악하였다. 또한 SSG와 기존의 LW 및 SGR의 일축압축강도와 비교하였다. 연구 결과, SSG가 상온 양생 및 저온 양생 모두에서 강도가 크게 측정되었으며, 초기 강도도 상대적으로 매우 우수한 것으로 나타났다.

• Steel and Composite Structures
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• 제44권5호
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• pp.741-752
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• 2022

Earlier works have shown that excessive shear stiffness at the steel-concrete interface causes a non-uniform distribution of shear force in composite structures. When the shear studs are wrapped at the fixed end with flexible materials with a low elastic modulus, the shear stiffness at the interface is reduced. The objective of this study was to investigate the effect of silicone rubber-sleeve mounted on shear studs on the shear stiffness of steel-concrete composite structures. Eighteen push-out tests were conducted to investigate the mechanical behavior of silicone rubber-sleeved shear stud groups (SRS-SSG). The dimension and arrangement of silicon rubber-sleeves (SRS) were taken into consideration. Test results showed that the shear strength of SRS-SSG was higher than that of a shear stud group (SSG), without SRS. For SRS-SSG with SRS heights of 50 mm, 100 mm, 150 mm, the shear strengths were improved by 13%, 20% and 9%, respectively, compared to the SSG alone. The shear strengths of SRS-SSG with the SRS thickness of 2 mm and 4 mm were almost the same. The shear stiffness of the SRS-SSG specimens with SRS heights of 50 mm, 100 mm and 150 mm were 77%, 67% and 66% of the SSG specimens, respectively. Test results of specimens SSG-1 and predicted values based on the three design specifications were compared. The nominal single stud shear strength of SSG-1 specimens was closest to that calculated by the Chinese Code for Design of Steel Structures (GB50017-2017). An equation is proposed to consider the effects of SRS for GB50017-2017, and the predicted values based on the proposed equation agree well with the tested results of SRS-SSG.

• BMB Reports
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• 제55권3호
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• pp.154-159
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• 2022

Protein S-glutathionylation is a reversible post-translational modification on cysteine residues forming a mixed disulfide with glutathione. S-glutathionylation, not only protects proteins from oxidation but also regulates the functions of proteins involved in various cellular signaling pathways. In this study, we developed a method for the detection of S-glutathionylated proteins (ProSSG) using eosin-glutathione (E-GSH) and mouse glutaredoxin 1 (mGrx1). ProSSG was efficiently and specifically labeled with E-GSH to form ProSSG-E via thiol-disulfide exchange. ProSSG-E was readily luminescent allowing the detection of ProSSG with semi-quantitative determination. In addition, a deglutathionylation enzyme mGrx1 specifically released E-GSH from ProSSG-E, which increased fluorescence allowing a sensitive determination of ProSSG levels. Application of the method to the adipocyte differentiation of 3T3-L1 cells showed specific detection of ProSSG and its increase upon differentiation induction, which was consistent with the result obtained by conventional immunoblot analysis, but with greater specificity and sensitivity.

• 한국지반공학회논문집
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• 제32권3호
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• pp.29-37
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• 2016

최근 개발된 자기치유 그라우팅(SSG, self-healing smart grouting)공법은 지반의 내구성 및 차수성의 향상, 용탈 및 환경오염 방지 등의 특성을 가지고 있다. 이 연구는 SSG공법의 공학적 특성을 규명한 것으로써 기존 약액주입(LW, SGR)공법과의 공학적 특성을 비교하기 위해 겔타임 측정, 일축압축강도 시험, 투수 시험, 어독성 시험, 내화학성 시험을 수행하였다. 연구결과, SSG공법은 장 단기 차수성능 유지, 강도 발현, 내구성 확보에 유리하고 용탈에 의한 지하수 및 지반의 오염 가능성이 낮은 것으로 나타났다. 이를 통해 SSG공법은 기존 약액주입공법의 문제점들을 최소화할 수 있는 대체 공법으로 현장에 적용이 가능할 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제17권3호
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• pp.454-460
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• 2007

Enzymatic properties and substrate specificity of recombinant $\beta-glycosidases$ from a hyperthermophilic archaeon, Sulfolobus shibatae (rSSG), were analyzed. rSSG showed its optimum temperature and pH at $95^{\circ}C$ and pH 5.0, respectively. Thermal inactivation of rSSG showed that its half-life of enzymatic activity at $75^{\circ}C$ was 15 h whereas it drastically decreased to 3.9 min at $95^{\circ}C$. The addition of 10 mM of $MnCl_2$ enhanced the hydrolysis activity of rSSG up to 23% whereas most metal ions did not show any considerable effect. Dithiothreitol (DTT) and 2-mercaptoethanol exhibited significant influence on the increase of the hydrolysis activity of rSSG rSSG apparently preferred laminaribiose $(\beta1\rightarrow3Glc)$, followed by sophorose $(\beta1\rightarrow2Glc)$, gentiobiose $(\beta1\rightarrow6Glc)$, and cellobiose $(\beta1\rightarrow4Glc)$. Various. intermolecular transfer products were formed by rSSG in the lactose reaction, indicating that rSSG prefers lactose as a good acceptor as well as a donor. The strong intermolecular transglycosylation activity of rSSG can be applied in making functional oligosaccharides.

• TTA 저널
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• 통권82호
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• pp.173-176
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• 2002

• TTA 저널
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• 통권88호
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• pp.205-211
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• 2003

• 동의생리병리학회지
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• 제19권1호
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• pp.218-223
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• 2005

According to the Chinese and Korean medicinal and herbal literature, SSG(Silsosangami) is effective for the treatment of inflammation, hyperlipemia and arteriosclerosis. The pharmacological action of SSG has been limitedly studied in regard to ischemic infarction. This herbal medicine has been shown to express diverse activities such as immunomodulating, anti-infarction, anti-allergic and anti-inflammatory effects. Antisclerotic effects of SSG in experimentally induced atherosclerosis in rabbits have also been reported. However, pharmacological mechanisms of SSG on lipid metabolism and atherosclerosis formation are poorly understood. The present paper reports the effect of extracts obtained from SSG on endotoxin-induced experimental DIC in rats. Also, these were tested for their effect on endotoxin-induced blood platelet aggregation, thrombin-induced conversion of fibrinogen and fibrinolysis in vitro experiments with aspirin as a positive agent. The anti-thrombic properties of SSG were also investigated by means of analytical parameters of bood composition. The extracts of SSG and its seven herbs, except Cnidii Rhizoma and Carthami Flos, inhibited the endotoxin-induced DIC and thrombosis in rats. Also the extract inhibited the endotoxin-induced decrease in blood platelets and fibrinogen, and endotoxin-induced increase in fibrin degradation products (FDP) on disseminated intravascular coagulation in normal rats.