• Journal of Microbiology and Biotechnology
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• 제24권9호
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• pp.1216-1225
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• 2014

Biofilm-related infections of Candida albicans are a frequent cause of morbidity and mortality in hospitalized patients, especially those with immunocompromised status. Options of the antifungal drugs available for successful treatment of drug-resistant biofilms are very few, and as such, new strategies need to be explored against them. The aim of this study was to evaluate the efficacy of phenylpropanoids of plant origin against planktonic cells, important virulence factors, and biofilm forms of C. albicans. Standard susceptibility testing protocol was used to evaluate the activities of 13 phenylpropanoids against planktonic growth. Their effects on adhesion and yeast-to-hyphae morphogenesis were studied in microplate-based methodologies. An in vitro biofilm model analyzed the phenylpropanoid-mediated prevention of biofilm development and mature biofilms using XTT-metabolic assay, crystal violet assay, and light microscopy. Six molecules exhibited fungistatic activity at ${\leq}0.5mg/ml$, of which four were fungicidal at low concentrations. Seven phenylpropanoids inhibited yeast-to-hyphae transition at low concentrations (0.031-0.5 mg/ml), whereas adhesion to the solid substrate was prevented in the range of 0.5-2 mg/ml. Treatment with ${\leq}0.5mg/ml$ concentrations of at least six small molecules resulted in significant (p < 0.05) inhibition of biofilm formation by C. albicans. Mature biofilms that are highly resistant to antifungal drugs were susceptible to low concentrations of 4 of the 13 molecules. This study revealed phenylpropanoids of plant origin as promising candidates to devise preventive strategies against drug-resistant biofilms of C. albicans.

• 대한원격탐사학회지
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• 제33권1호
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• pp.69-77
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• 2017

S The need for an automated geo-positioning approach for near real-time results and to boost cost-effectiveness has become increasingly urgent. Following this trend, a new approach to automatically compensate for the bias of the rational function model (RFM) was proposed. The core idea of this approach is to remove the bias of RFM only using tie points, which are corrected by matching with the digital elevation model (DEM) without any additional ground control points (GCPs). However, there has to be a additional evaluation according to the quality of DEM because DEM is used as a core element in this approach. To address this issue, this paper compared the quality effects of DEM in the conduct of the this approach using the Shuttle Radar Topographic Mission (SRTM) DEM with the spatial resolution of 90m. and the National Geographic Information Institute (NGII) DEM with the spatial resolution of 5m. One KOMPSAT-2 stereo-pair image acquired at Busan, Korea was used as experimental data. The accuracy was compared to 29 check points acquired by GPS surveying. After bias-compensation using the two DEMs, the Root Mean Square (RMS) errors were less than 6 m in all coordinate components. When SRTM DEM was used, the RMSE vector was about 11.2m. On the other hand, when NGII DEM was used, the RMSE vector was about 7.8 m. The experimental results showed that automated geo-positioning approach can be accomplished more effectively by using NGII DEM with higher resolution than SRTM DEM.

• Journal of Microbiology and Biotechnology
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• 제27권4호
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• pp.685-693
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• 2017

Candidiasis involving the biofilms of Candida albicans is a threat to immunocompromised patients. Candida biofilms are intrinsically resistant to the antifungal drugs and hence novel treatment strategies are desired. The study intended to evaluate the anti-Candida activity of allyl isothiocyanate (AITC) alone and with fluconazole (FLC), particularly against the biofilms. Results revealed the concentration-dependent activity of AITC against the planktonic growth and virulence factors of C. albicans. Significant (p <0.05) inhibition of the biofilms was evident at ${\leq}1mg/ml$ concentrations of AITC. Notably, a combination of 0.004 mg/ml of FLC and 0.125 mg/ml of AITC prevented the biofilm formation. Similarly, the preformed biofilms were significantly (p <0.05) inhibited by the AITC-FLC combination. The fractional inhibitory concentration indices ranging from 0.132 to 0.312 indicated the synergistic activity of AITC and FLC against the biofilm formation and the preformed biofilms. No hemolytic activity at the biofilm inhibitory concentrations of AITC and the AITC-FLC combination suggested the absence of cytotoxic effects. The recognizable synergy between AITC and FLC offers a potential therapeutic strategy against biofilm-associated Candida infections.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2008년도 International Symposium on Remote Sensing
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• pp.196-199
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• 2008

Lineament is defined generally as a linear feature or pattern on interpretation of a satellite image and indicates the geological structures such as faults and fractures. For this reason, a lineament extraction and analysis using remote sensing images have been widely used for mapping large areas. The Gyeong-gang Fault is a NNE trending structure located in Gangwon-do and Kyeonggi-do district. However, a few geological researches on that fault have been carried out and its trace or continuity is ambiguous. In this study, we investigate the geologic features at Gyeong-gang Fault Zone using LANDSAT ETM+ satellite image and SRTM digital elevation model. In order to extract the characteristics of geologic features effectively, we transform the LANDSAT ETM+ image using Principal Component Analysis (PCA) and create a shade relief from SRTM data with various illumination angles. The results show that it is possible to identify the dimensions and orientations of the geologic features at Gyeong-gang Fault Zone using remote sensing data. An aerial photograph interpretation and a field work will be future tasks for more accurate analysis in this area.

• 한국측량학회지
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• 제32권3호
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• pp.217-223
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• 2014

With precise sensor position, attitude element, and imaging resolution, a simulated geospatial image can be generated. In this study, a satellite image is simulated using SPOT ortho-image and global elevation data, and the geometric similarity between original and simulated images is analyzed. Using a SPOT panchromatic image and high-density elevation data from a 1/5K digital topographic map data an ortho-image with 10-meter resolution was produced. The simulated image was then generated by exterior orientation parameters and global elevation data (SRTM1, GDEM2). Experimental results showed that (1) the agreement of the image simulation between pixel location from the SRTM1/GDEM2 and high-resolution elevation data is above 99% within one pixel; (2) SRTM1 is closer than GDEM2 to high-resolution elevation data; (3) the location of error occurrence is caused by the elevation difference of topographical objects between high-density elevation data generated from the Digital Terrain Model (DTM) and Digital Surface Model (DSM)-based global elevation data. Error occurrences were typically found at river boundaries, in urban areas, and in forests. In conclusion, this study showed that global elevation data are of practical use in generating simulated images with 10-meter resolution.

• 한국측량학회지
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• 제32권1호
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• pp.19-27
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• 2014

2012년 5월 발사된 다목적 실용위성 아리랑 3호는 단일 패스 상에서 0.7m의 공간해상도로 스테레오 영상을 획득할 수 있어 기존의 아리랑 2호에 비해 고품질의 Digital Elevation Model(DEM) 추출이 가능하다. 통상적으로 DEM 추출을 위해서는 영상 전반에 걸쳐 골고루 취득된 정밀한 기준점을 사용하여 센서모델링을 수행하고, 스테레오 매칭을 수행해야하나, 해외 지역이나 접근이 힘든 지역 등 GPS측량이 쉽지 않은 지역의 경우에는 무기준점 또는 기준점을 최소화하거나 기 구축된 공간 데이터를 활용하는 등의 방법으로 DEM을 추출해야 한다. 따라서 본 연구에서는 아리랑 3호 데이터로부터 무기준점 기반, 상대표정 기반, 단일 기준점 등 여러 가지 Rational Polynomial Coefficients(RPC) 처리 조건에 따라 DEM을 생성하고 정확도를 평가하였다. 기 구축된 공간영상인 Digital Orthophoto Quadrangle(DOQ) 와 Shuttle Radar Topography Mission(SRTM)을 기준점 자료로 활용하여 미국지역 아리랑3호 스테레오 데이터를 대상으로 DEM을 생성하였으며, LiDAR DEM을 이용하여 정확도를 평가하였다. 실험 결과 무기준점인 경우 상대표정을 통해 의미 있는 정확도 향상을 얻을 수 있었고, DOQ와 SRTM 조합의 단일 기준점으로도 영상 전반에 걸쳐 기준점을 획득한 경우에 근접하는 7m 정도의 DEM 정확도를 확보할 수 있었다.

• 한국지반환경공학회 논문집
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• 제17권9호
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• pp.21-28
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• 2016

본 연구의 목적은 유역규모별 홍수침수면적을 산정하기 위한 하천지형 구축기법과 그에 따른 침수면적을 비교하는 것이다. 대상 지역은 경상북도 청도군과 의성군 내 14개소 하천이며 수치지도 DEM, ASTER DEM과 SRTM DEM을 이용하여 하천지형을 구축하였으며, HEC-GeoRAS, RAS Mapper, RiverCAD 모형을 적용하여 홍수침수해석을 수행하였다. 해석 결과와 실제 침수흔적도와 비교하였으며, 각 해석 모형들과 하천지형 구축기법에 따라 침수면적을 비교하였다. 침수흔적도와 비교한 결과 HEC-GeoRAS 모형의 결과가 이 침수흔적도의 범람면적과 대부분의 유역에서 유사한 값을 나타내었으며, SRTM DEM이 ASTER DEM보다 수치지도 DEM과 유사한 결과를 나타내었다. 중 대규모 유역의 경우 HEC-GeoRAS 모형과 Ras Mapper 모형, 소규모 유역의 경우 HEC-GeoRAS 모형과 RiverCAD 모형이 효율적인 해석 모형으로 나타났다.

• 한국측량학회:학술대회논문집
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• 한국측량학회 2006년도 춘계학술발표회 논문집
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• pp.277-280
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• 2006

항공사진이나 인공위성 영상을 이용하여 DEM을 생성하는 연구는 전통적으로 사진측량학 분야에서 이루어져 왔다. 즉, 항공기 및 위성을 이용하여 획득한 입체의 영상자료를 이용하여 DEM을 생성하는 기법은 전통적으로 행해져 왔고 최근에 들어서는 LIDAR를 이용하여 1m 급 이상의 정밀 DEM이 획득되고 있다. 그러나 자국 이외 지역에 대한 DEM 자료를 획득하는 일은 위성 및 항공기를 이용한 입체쌍의 영상자료, 기준점 등의 자료를 얻기가 힘들기 때문에 공간해상도가 90m인 USGS에서 제공하는 SRTM자료를 활용해야 하는 등 제한적이다. 이에, 본 연구에서는 공간해상도 15m의 DEM 생성이 가능한 ASTER 영상을 이용하여 중국지역에 대한 정밀 DEM을 생성하고자 하였다. ASTER 영상은 가시광선대, 적외선대 및 열밴드의 정보를 제공하고 있을 뿐만 아니라 DEM 제작을 위하여 위성진행 경로에 정방향 및 역방향의 입체 영상을 제공하고 있다. 이러한 ASTER 영상의 센서 정보와 접합점을 이용하여 DEM을 생성하였고, 이를 SRTM 자료와 동기화 하여 두 자료를 비교 분석하였다.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2008년도 International Symposium on Remote Sensing
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• pp.212-215
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• 2008

The final product generated from the KOMPSAT-2 Terminal for Polar System, K2PS, is an ellipsoid projected image. This leaves a relief displacement on the image by process of which the height value of subject area is constant. In this paper, orthorectification using the SRTM was used to remove such artifacts, and thereafter, the additional product that could be generated from the K2PS was discussed.

• 대한원격탐사학회지
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• 제24권5호
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• pp.483-496
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• 2008

Qualified ground control points (GCPs) are required to construct a digital elevation model (DEM) from a pushbroom stereo pair. An inverse geolocation algorithm for extracting GCPs from ERS SAR data and the SRTM DEM was recently developed. However, not all GCPs established by this method are accurate enough for direct application to the geometric correction of pushbroom images such as SPOT, IRS, etc, and thus a method for selecting and removing inaccurate points from the sets of GCPs is needed. In this study, we propose a method for evaluating GCP accuracy and winnowing sets of GCPs through orientation modeling of pushbroom image and validate performance of this method using SPOT stereo pair of Daejon City. It has been found that the statistical distribution of GCP positional errors is approximately Gaussian without bias, and that the residual errors estimated by orientation modeling have a linear relationship with the positional errors. Inaccurate GCPs have large positional errors and can be iteratively eliminated by thresholding the residual errors. Forty-one GCPs were initially extracted for the test, with mean the positional error values of 25.6m, 2.5m and -6.1m in the X-, Y- and Z-directions, respectively, and standard deviations of 62.4m, 37.6m and 15.0m. Twenty-one GCPs were eliminated by the proposed method, resulting in the standard deviations of the positional errors of the 20 final GCPs being reduced to 13.9m, 8.5m and 7.5m in the X-, Y- and Z-directions, respectively. Orientation modeling of the SPOT stereo pair was performed using the 20 GCPs, and the model was checked against 15 map-based points. The root mean square errors (RMSEs) of the model were 10.4m, 7.1m and 12.1m in X-, Y- and Z-directions, respectively. A SPOT DEM with a 20m ground resolution was successfully constructed using a automatic matching procedure.