• The Journal of Korean Society of Virology
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• v.29 no.4
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• pp.247-259
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• 1999

Small round structured viruses (SRSV) are the major ethological agents which can cause outbreaks of non-bacterial gastroenteritis or food poisoning both in children and adults. The classification of family Caliciviridae to which SRSV belong, is based on the genome encoding three open reading frames. The rotavirus is another major pathogen which causes diarrhea in young children. We examined stool specimens obtained from diarrheal patients in Wonju from which bacterial pathogens were not found. To detect causative viruses from stool specimens of patients, reverse transcription (RT)-polymerase chain reaction (PCR) or nested PCR using rotavirus or SRSV specific primers was performed. In this study, RT-nested PCR procedure which can amplify a 330 bp fragment derived from RNA dependent RNA polymerase (RDRP) region within ORF1 was applied for the detection of SRSV. For the detection of rotaviruses, a 877 bp fragment from the VP4 region of rotavirus genome was amplified. As a result, rotavirus was not detected while SRSV sequences were detected from one out of five specimens. The nucleotide and amino acid sequences of the Wonju isolate were compared with other 6 Korean isolates which have been isolated and sequenced in our laboratory. Sequence analysis revealed that the Wonju isolate was rather distinct from other Korean isolates: the Wonju isolate was closer to genogroup I of SRSV while other 6 Korean isolates belonged to genogroup II.

• Korean Journal of Microbiology
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• v.37 no.4
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• pp.289-293
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• 2001

Incidence of infectious viruses is ensuing throughout the world and threatening the health of children as well as adults. The outbreaks of viral diseases of alimentary tract in Pusan from 1998 to 2000 were detected. Viruses were isolated from stool specimens, cerebrospinal fluid and throat swabs from suspicious patients and confirmed by cell culture, latex agglutination test, indirect immunofluorescent test and electron microscopic observation. The average isolation rate was 12.5% from the suspected specimens. From this work, 2 cases of enteric adenoviruses, 23 cases of echovirus, 31 cases of coxsackivirus 36 cases of rotavirus, 45 cases of SRSV, and 7 cases of poliovirus were detected. The major serotypes of coxsackievirus were B2, B3, B4, B6 and echovirus of serotypes 6, 9, 11, 25, and 30 were examined. Two cases of enteric adenovirus type 41 were also confirmed. The incidence of SRSV was mostly concentrated between December through following March, April through October with echovirus and coxsackievirus, and January through April with rotavirus, respectively. Electron micrograph of negative-stained viruses showed typical appearance with 30-80 nm in diameter.

• Korean Journal of Microbiology
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• v.37 no.4
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• pp.294-298
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• 2001

Highly effective polymerase chain reaction (PCR) often brings about false positivity caused by contamination of the sample with target nucleic acids. To solve this problem, in situ PCR (ISPCR) has been developed and applied onto various tissue sections and suspension cultures. With combination of PCR and in situ hybridization, this method amplifies the nucleic acid targets in situ and detect the amplified products inside the cells over the background of various cell types. In order to amplify the nucleic acid targets inside the cells, permeabilisation of a sample is required for the entry of amplification reactants into a cell. Treatments of a sample for the purpose allow not only the entry of reactants into the cell but also the exit of amplification products out of the cell. As a means to reduce the leakage of the amplification products, two methods were applied to suspension cultures of HIV-infected Molt/LAV and U 1.1 cells, in which modified, tailed primers produced long linear amplificants whereas biotinylated dUTP instead of dTTP did bulky products.

• Journal of Environmental Health Sciences
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• v.33 no.3
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• pp.184-189
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• 2007

Pathogenic microbial foodborne disease outbreaks (PMFBDOs) have increased in many countries, the boom in food service establishment is not matched by effective food safety and control. In this study, we investigated the current state and the epidemic aspects of FBDOs in Korea and Japan. In Korea, the average prevalence rate of foodborne disease (FBD) was 15.0 per 100,000 population and cases per outbreak of FBD was 57.0. During the same period in Japan, the prevalence rate showed an average of 24.9, and the cases per outbreak were 16. When both prevalence rate and cases per outbreak were compared, the prevalence rate in Japan was much higher than that in Korea (p<0.01). However, average cases per outbreak of FBD in Japan were much lower than those in Korea (p<0.01). In Korea, outbreaks of FBDs were more common in spring (p<0.01), while in Japan, more frequent in summer and winter (p<0.01). Outbreaks of FBD occurred largely through restaurant and school foods (32.0% and 27.5%) in Korea. In Japan, the proportion of the outbreak cases in the restaurant and home were 23.7% and 12.1%, and cases of unknown causes of FBDs were 48.2%, respectively. Bacteria were the major causes of infection in both countries. The prevalence of PMFBDOs by Salmonella spp. Vibrio parahemolyticus and Staphylococcus aureus were much higher in Korea, while those by Camphylobacter spp. and SRSV were more common in Japan. The causes by virus were more frequent in Japan (13.7%) than in Korea (7.7%). The prevalence of FBDs in Korea and Japan showed characteristic differences, especially in the PMFBDOs due to such factors as geography, climate, culture, diet and food management.

• KIPS Transactions on Software and Data Engineering
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• v.1 no.3
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• pp.137-148
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• 2012

Cloud service users request dedicated virtual computing resource from the cloud service provider to process jobs in independent environment from other users. To optimize this process with automated method, in this paper we proposed a framework for workflow scheduling in the cloud environment, in which the core component is the middleware called broker mediating the interaction between users and cloud service providers. To process jobs in on-demand and virtualized resources from cloud service providers, many papers propose scheduling algorithms that allocate jobs to virtual machines which are dedicated to one machine one job. With this method, the isolation of being processed jobs is guaranteed, but we can't use each resource to its fullest computing capacity with high efficiency in resource utilization. This paper therefore proposed a cost-efficient job scheduling algorithm which maximizes the utilization of managed resources with increasing the degree of multiprogramming to reduce the number of needed virtual machines; consequently we can save the cost for processing requests. We also consider the performance degradation in proposed scheme with thrashing and context switching. By evaluating the experimental results, we have shown that the proposed scheme has better cost-performance feature compared to an existing scheme.