Sequence Analysis of Small Round Structured Viruses (SRSV) Isolated from a Diarrheal Patient in Wonju

원주지역 설사 환자에서 분리한 Small Round Structured Viruses (SRSV) 염기서열 분석

  • Jee, Young-Mee (Laboratory of Enteroviruses, Department of Virology, National Institute of Health) ;
  • Kim, Ki-Soon (Laboratory of Enteroviruses, Department of Virology, National Institute of Health) ;
  • Cheon, Doo-Sung (Laboratory of Enteroviruses, Department of Virology, National Institute of Health) ;
  • Park, Jeong-Koo (Laboratory of Enteroviruses, Department of Virology, National Institute of Health) ;
  • Kang, Young-Hwa (Laboratory of Enteroviruses, Department of Virology, National Institute of Health) ;
  • Chung, Yoon-Suck (Laboratory of Enteroviruses, Department of Virology, National Institute of Health) ;
  • Go, Un-Yeong (Division of Epidemiology, Department of Infectious Diseases, National Institute of Health) ;
  • Shin, Young-Hack (Division of Epidemiology, Department of Infectious Diseases, National Institute of Health) ;
  • Yoon, Jae-Deuk (Laboratory of Enteroviruses, Department of Virology, National Institute of Health)
  • 지영미 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 김기순 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 천두성 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 박정구 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 강영화 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 정윤석 (국립보건원 바이러스질환부 소화기계바이러스과) ;
  • 고운영 (국립보건원 감염질환부 역학조사과) ;
  • 신영화 (국립보건원 감염질환부 역학조사과) ;
  • 윤재득 (국립보건원 바이러스질환부 소화기계바이러스과)
  • Published : 1999.12.30

Abstract

Small round structured viruses (SRSV) are the major ethological agents which can cause outbreaks of non-bacterial gastroenteritis or food poisoning both in children and adults. The classification of family Caliciviridae to which SRSV belong, is based on the genome encoding three open reading frames. The rotavirus is another major pathogen which causes diarrhea in young children. We examined stool specimens obtained from diarrheal patients in Wonju from which bacterial pathogens were not found. To detect causative viruses from stool specimens of patients, reverse transcription (RT)-polymerase chain reaction (PCR) or nested PCR using rotavirus or SRSV specific primers was performed. In this study, RT-nested PCR procedure which can amplify a 330 bp fragment derived from RNA dependent RNA polymerase (RDRP) region within ORF1 was applied for the detection of SRSV. For the detection of rotaviruses, a 877 bp fragment from the VP4 region of rotavirus genome was amplified. As a result, rotavirus was not detected while SRSV sequences were detected from one out of five specimens. The nucleotide and amino acid sequences of the Wonju isolate were compared with other 6 Korean isolates which have been isolated and sequenced in our laboratory. Sequence analysis revealed that the Wonju isolate was rather distinct from other Korean isolates: the Wonju isolate was closer to genogroup I of SRSV while other 6 Korean isolates belonged to genogroup II.

Keywords