• Journal of Ginseng Research
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• v.22 no.2
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• pp.126-132
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• 1998

In the present study, we examined effects of ginseng saponins (ginsenosides) on pp60c-src protein tyrosine kinase (PTK) activity, intracellular calcium concentration ([$Ca^{2+}$]i), and cell proliferation in NIH3T3 cells. Eight different ginsenosides [ginsenoside-Rb1 (G-$Rb_1$), -$Rb_2$, -Rc, -Rd, -Re, -Rf, -$Rg_1$, -$Rg_2$) and ginseng total saponin (GTS) were used for these experiments. All ginsenosides and GTS tested stimulated the activation of $pp60^{c-src}$ kinase, and especially G-$Rb_1$,-Rd,-$Rg_1$, and -$Rg_1$ showed a higher stimulatory effect than others at 16.7 $\mu\textrm{g}$/ml of ginsenosides with a 18 hr-incubation, increasing the activity by 4.5, 3.5, 3.5, and 3.0-fold, respectively, over that of untreated control. In addition, both G-Rd and -$Rg_2$)Rg2 increased ($Ca^{2+}$), to 202 and 334 nM, respectively, about 2-3-fold above the basal level within 7min at 250 $\mu\textrm{g}$/yml of ginsenosides. The increases of ($Ca^{2+}$), were eliminated by Pretreatment of EGTA, an extracellular calcium chelator, suggtasting that they result from an influx of calcium ion from extracellular medium rather than an efflux from intracellular calcium store, endoplasmic reticulum (ER). All ginsenosides studied enhanced cell proliferation to 1.2-1.4-fold over that of untreated control at 5~250 $\mu\textrm{g}$/ml of concentrations. Interestingly the promotion of cell proliferation by ginsenosides corresponded with the activation of c-src kinase, which is an early step in the mitogenic signaling cascade. Taken together, we suggest that some ginsenosides may lead to cellProliferation via the activation of cellular signal transduction Pathway involving $pp60^{c-src}$ kinase.

• YAKHAK HOEJI
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• v.52 no.5
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• pp.370-375
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• 2008

In this study, the anti-allergic activity and mechanim of Salviae radix (SR) were investigated. The ethanol extract of SR showed significant inhibitory effect on degranulation from antigen-stimulated mast cells and it also inhibited the expression and secretion of TNF-${\alpha}$ and IL-4 in antigen-stimulated RBL-2H3 cells. In the mast cell-mediated local animal allergy model, it suppressed the passive cutaneous anaphylaxis in a dose-dependent manner. As its mechanism of action, SR inhibited the activating phosphorylation of Syk, a downstream signaling molecule of Src-family kinase, for the activation of mast cells. The results of the study indicate that the anti-allergic activity of SR is mediated by the inhibition of Src-family kinase in mast cells.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.11a
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• pp.97-100
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• 2004

Steel encased composite columns are widely used due to their excellent structural performance in terms of stiffness, strength, and ductility. However, experimental studies were usually for the columns having higher steel ratio $(3-4\%)$. There are two different design concepts for SRC columns. ACI-318 specifies the design strength of the column using the same concept of reinforced concrete columns. AISC-LRFD specifies the P-M diagram using the concept of steel column. In this paper, SRC columns have the steel ratio of $0.53\%\;and\;1.06\%$. From the test results, ACI-318 specifications showed better evaluation of SRC columns having low steel ratio. H beam and steel tube partially filled with concrete were embedded in concrete. Flexural tests showed considerably high ductility.

• Journal of the Korean Magnetic Resonance Society
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• v.14 no.2
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• pp.105-116
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• 2010

SH3YL1, a novel protein containing one Src homology 3 domain at the carboxyl terminus was first detected in mouse anagen skin cDNA. This protein had a significant homology with YHRO 16c/Ysc 84, the yeast Src homology 3 domain-containing protein. The sequence identity was remarkable at the carboxyl and amino-terminal Src homology 3 domain, suggesting that the novel protein is a mouse homolog of the yeast protein and thus was termed as SH3YL1. SH3YL1 is composed of two domains, a DUF500 at N-termini and a SH3 domain at C-termini. In our study we cloned the SH3 domain in bacterial expression system in Escherichia coli using pET32a vector with TEV protease cleavage site and purified as a monomer using affinity chromatography. The N-terminal poly-Histidine tag was cleaved with TEV protease and target protein was used for backbone studies. Our study showed that SH3 domain primarily consists of $\beta$-sheet which is in consistence with previous result performed on the truncated SH3 domain of SH3YL1.

• Earthquakes and Structures
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• v.7 no.3
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• pp.317-331
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• 2014

An experimental study was performed to investigate the seismic performance of solid steel reinforced concrete (SRC) frames with special-shaped columns that are composed of SRC special-shaped columns and reinforced concrete beams. For this purpose, two models of two-bay and three-story frame, including an edge frame and a middle frame, were designed and tested. The failure process and patterns were observed. The mechanical behaviors such as load-displacement hysteretic loops and skeleton curves, load bearing capacity, drift ratio, ductility, energy dissipation and stiffness degradation of test specimens were analyzed. Test results show that the failure mechanism of solid SRC frame with special-shaped columns is the beam-hinged mechanism, satisfying the seismic design principle of "strong column and weak beam". The hysteretic loops are plump, the ductility is good and the capacity of energy dissipation is strong, indicating that the solid SRC frame with special-shaped columns has excellent seismic performance, which is better than that of the lattice SRC frame with special-shaped columns. The ultimate elastic-plastic drift ratio is larger than the limit value specified by seismic code, showing the high capacity of collapse resistance. Compared with the edge frame, the middle frame has higher carrying capacity and stronger energy dissipation, but the ductility and speed of stiffness degradation are similar. All these can be helpful to the designation of solid SRC frame with special-shaped columns.

• The Korean Journal of Physiology and Pharmacology
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• v.25 no.2
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• pp.159-166
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• 2021

Nicotinamide adenine dinucleotide phosphate oxidases (NOXs) are the major enzymatic source of reactive oxygen species (ROS). NOX2 and NOX4 are expressed in the heart but its role in hypoxia-induced atrial natriuretic peptide (ANP) secretion is unclear. This study investigated the effect of NOX on ANP secretion induced by hypoxia in isolated beating rat atria. The results showed that hypoxia significantly upregulated NOX4 but not NOX2 expression, which was completely abolished by endothelin-1 (ET-1) type A and B receptor antagonists BQ123 (0.3 μM) and BQ788 (0.3 μM). ET-1-upregulated NOX4 expression was also blocked by antagonists of secreted phospholipase A2 (sPLA2; varespladib, 5.0 μM) and cytosolic PLA2 (cPLA2; CAY10650, 120.0 nM), and ET-1-induced cPLA2 expression was inhibited by varespladib under normoxia. Moreover, hypoxia-increased ANP secretion was evidently attenuated by the NOX4 antagonist GLX351322 (35.0 μM) and inhibitor of ROS N-Acetyl-D-cysteine (NAC, 15.0 mM), and hypoxia-increased production of ROS was blocked by GLX351322. In addition, hypoxia markedly upregulated Src expression, which was blocked by ET receptors, NOX4, and ROS antagonists. ET-1-increased Src expression was also inhibited by NAC under normoxia. Furthermore, hypoxia-activated extracellular signal-regulated kinase 1/2 (ERK1/2) and protein kinase B (Akt) were completely abolished by Src inhibitor 1 (1.0 μM), and hypoxia-increased GATA4 was inhibited by the ERK1/2 and Akt antagonists PD98059 (10.0 μM) and LY294002 (10.0 μM), respectively. However, hypoxia-induced ANP secretion was substantially inhibited by Src inhibitor. These results indicate that NOX4/Src modulated by ET-1 regulates ANP secretion by activating ERK1/2 and Akt/GATA4 signaling in isolated beating rat hypoxic atria.

• Journal of Nutrition and Health
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• v.53 no.1
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• pp.1-12
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• 2020

Purpose: Diabetic nephropathy is one of the most important diabetic complications prompted by chronic hyperglycemia, characterized by glomerulosclerosis, tubular fibrosis, and it eventually causes kidney failure. Nobiletin is a polymethoxyflavone present in tangerine and other citrus peels, and has anti-cancer and anti-inflammatory effects. This study investigated the effects of nobiletin on glomerular fibrosis through inhibition of the transforming growth factor (TGF)-β1-Src-caveolin-1 pathway. Methods: Human renal mesangial cells (HRMC) were incubated in media containing 33 mM glucose with or without 1-20 uM nobiletin for 3 day. The cellular expression levels of fibrogenic collagen IV, fibronectin, connective tissue growth factor (CTGF), TGF-β1, Src and caveolin-1 were all examined. In addition, TGF-β1, Src and caveolin-1 proteins were screened to reveal the relationship among TGF-β1-Src-caveolin-1 signaling in glomerular fibrosis. Results: High glucose promoted the production of collagen IV, fibronectin and CTGF in HRMC, which was inhibited in a dose dependent manner by 1-20 uM nobiletin. The Western blot data showed that high glucose elevated the expression of TGF-β1, Src, caveolin-1 and Rho GTPase. When nobiletin was treated to the HRMC exposed to high glucose, the expression of TGF-β1-Src-caveolin-1 was dampened. Finally, TGF-β1-Src-caveolin-1 signaling pathway was activated in high glucose-exposed HRMC, and such activation was encumbered by nobiletin. Conclusion: These result demonstrated that nobiletin blunted high glucose-induced extracellular matrix accumulation via inhibition of the TGF-β1-Src-caveolin-1 related intracellular signaling pathway. Nobiletin may be a potent renoprotective agent to counteract diabetes-associated glomerular fibrosis that leads to kidney failure.

• Journal of the Korean Home Economics Association
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• v.42 no.6
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• pp.1-12
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• 2004

This study investigates residents' living satisfaction with high-rise, mixed-use apartments built in the structures of reinforced concrete construction(RC) and with steel framed, reinforced concrete construction(SRC). The following study results were obtained: 1) Residents expressed below average satisfaction (below 3.0) with the common-area landscape and environmentally-friendly factors, children's playground and its facilities, community service for residents' by the management offices and utility plan. 2) According to the satisfaction of sound environment, residents of the SRC group expressed above average satisfaction. It can be concluded that noise problems around the residential area of the SRC group was well solved in this study. However, the residents of the SRC group expressed low satisfaction with the freshness of indoor air, humidity, and smell in the living quarters. 3) High-rise, mixed-use apartments should accommodate a familial life cycle, be designed as an environmentally friendly resting place in harmony with city life, and provide various communal programs for residents.

• Steel and Composite Structures
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• v.21 no.3
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• pp.661-677
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• 2016

The quasi static test of the steel reinforced concrete (SRC) bridge piers and rigid frame arch bridge structure with SRC piers was conducted in the laboratory, and the seismic performance of SRC piers was compared with that of reinforced concrete (RC) bridge piers. In the test, the failure process, the failure mechanism, hysteretic curves, skeleton curves, ductility coefficient, stiffness degradation curves and the energy dissipation curves were analyzed. According to the $M-{\Phi}$ relationship of fiber section, the three-wire type theoretical skeleton curve of the lateral force and the pier top displacement was proposed, and the theoretical skeleton curves are well consistent with the experimental curves. Based on the theoretical model, the effects of the concrete strength, axial compression ratio, slenderness ratio, reinforcement ratio, and the stiffness ratio of arch to pier on the skeleton curve were analyzed.

• Steel and Composite Structures
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• v.47 no.4
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• pp.539-550
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• 2023

Owing to the obstruction of section steel on the moisture diffusion in concrete, the existing shrinkage prediction models overestimate the time-dependent deformation of steel reinforced concrete (SRC) columns, particularly for the SRC columns with enclosed section steels. To solve this issue, this study deals with analytical and experimental studies on the drying shrinkage for this type of column. First, an effective method for predicting the drying shrinkage of concrete based on finite element model is introduced and two crucial parameters for simulation of humidity field are determined. Then, the drying shrinkage of SRC columns with enclosed section steels is investigated and two modified parameters, which depend on the ambient relative humidity and the ratio of section steel size to column size, are introduced to the B3 model. Finally, an experiment on the shrinkage deformation of SRC columns with enclosed section steels is conducted. Comparing the predicted results with the experimental ones, it demonstrates that the modified B3 model is quite reasonable.