• Title/Summary/Keyword: SPIN90

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Regulation of SPIN90 by Cell Adhesion and ERK Activation

  • Kim Sung Hyun;Kim Dae Joong;Song Woo Keun
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2004.05a
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    • pp.141-146
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    • 2004
  • SPIN90 was identified to farm molecular complex with $\betaPIX$, WASP and Nck. This complex shows that SPIN90 interacts with Nck in a manner dependent upon cell adhesion to extracellular matrix, but $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex was stable even in suspended cells. This suggests that SPIN90 serves as an adaptor molecule to recruit other proteins to Nck at focal adhesions. SPIN90 was phosphorylated by ERK1, which was, itself, activated by cell adhesion and platelet-derived growth factor. Such phosphorylation of SPIN90 likely promotes the interaction of the $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex and Nck. It thus appears that the interaction of the $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex with Nck is crucial for stable cell adhesion and can be dynamically modulated by SPIN90 phosphorylation that is dependent on cell adhesion and ERX activation. SPIN90 directly binds syndapin I, syndapin isoform II-1 and II-s via its PRD region in vitro, in vivo and also associates with endocytosis core components such as clathrin and dynamin. In neuron and fibroblast, SPIN90 colocalizes with syndapins as puntate form, consistent with a role for SPIN90 in clathrin-mediated endocytosis pathway. Overexpression of SPIN90 N-term inhibits receptor-mediated endocytosis. Interestingly, SPIN90 PRD, binding interface of syndapin, significantly blocks internalization of transferrin, demonstrating SPIN90 involvement in endocytosis in vivo by interacting syndapin. Depletion of endogenous SPIN90 by introducing $\alpha-SPIN90$ also blocks receptor-mediated endocytosis. Actin polymerization could generate farce facilitating the pinch-out event in endocytosis, detach newly formed endocytic vesicle from the plasma membrane or push out them via the cytosol on actin tails. Here we found that SPIN90 localizes to high actin turn over cortical area, actin-membrane interface and membrane ruffle in PDGF treated cells. Overexpression of SPIN90 has an effect on cortical actin rearrangement as filopodia induction and it is mediated by the Arp2/3 complex at cell periphery. Consistent with a role in actin organization, CFP-SPIN90 present in actin comet tail generated by PIP5 $kinase\gamma$ overexpression. Therefore this study suggests that SPIN90 is functional linker between endocytosis and actin cytoskeleton.

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Magnetoresistive of (NiFe/CoFe)/Cu/CoFe Spin-Valvec ((NiFe/CoFe)/Cu/CoFe Spin-Valve 박막의 자기저항 특성)

  • 오미영;이선영;이정미;김미양;이장로
    • Journal of the Korean Magnetics Society
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    • v.7 no.5
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    • pp.265-273
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    • 1997
  • The MR ratios and the exchange biasing field and interlayer coupling field were investigated in $Ni_{91}Fe_{19}/Co_{90}Fe_{10}/Cu/Co_{90}Fe_{10}/NiO$ spin-valve sandwiches grown on antiferromagnetic NiO films as a function of the NiO thickness, the thickness of Cu and pinning layer $Co_{90}Fe_{10}$. The spin-valve sandwiches were deposited on the Corning glass 7059 by means of the 3-gun dc and 1-gun rf magnetron sputtering at a 5 mtorrpartial Ar pressure and room temperature. The deposition field was 50 Oe. The MR curve was measured by the four-terminal method with applied magnetic soft bilayer [NiFe/CoFe] (90$\AA$) decreased dramatically to less than 10 Oe when the NiFe/CoFe bilayer used an NiFe bilayer thicker that 20$\AA$. So NiFe layer improved the softmagnetic properties in the NiFe/CoFe bilayer. The GMR ratio and the magnetic field sensitivity of the spin-valve film $Ni_{91}Fe_{19}(40{\AA})/Co_{90}Fe_{10}(50{\AA}) /Cu(30{\AA})/Co_{90}Fe_{10}(35{\AA})/NiO(800{\AA})$ was 6.3% and about 0.5 (%/Oe), respectively. The MR ratio had 5.3% below an annealing temperature of 20$0^{\circ}C$ which slowly decreased to 3% above 30$0^{\circ}C$. The large blocking temperature of the spin-valve film was taken (as being) due to the good stability of the NiO films. Thus, the spin-valve films with a free NiFe/CoFe layer clearly had a high large GMR output and showed a effective magnetic field sensitivity for a suitable spin-valve head material.

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Current-induced Spin Wave Excitations in Asymmetric Nanopillar Junctions

  • Fiandimas, Arie;Lee, Kyung-Jin;Shin, Kyung-Ho
    • Journal of Magnetics
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    • v.14 no.2
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    • pp.90-92
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    • 2009
  • This study examined the current-induced spin wave excitation in asymmetric nanopillar junctions with a stack sequence of 20 nm Pt/10 nm Cu/7 nm NiFe/300 nm Cu, and a circular lateral dimension of 240 nm. An analysis of the magnetic and magnetotransport characteristics of the junction showed a possible spin transfer effect at this sample dimension when the magnetization was switched from a vortex state to another state. This finding is expected to help improve the understanding of the spin transfer torque phenomenon in nanopillar junctions.

A Possible Origin of Ferromagnetism in Epitaxial BiFeO3 thin Films

  • Chang, Jae-wan;Jang, Hyun M.;Kim, Sang-Koog
    • Journal of Magnetics
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    • v.11 no.3
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    • pp.108-110
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    • 2006
  • We successfully enhanced the performance of a spin valve by inserting an ultra-thin layer of partially oxidized Fe in the pinned and free layers. With the exchange bias field kept large, the spin valve reached a GMR of 12%, which corresponded to a 55% increase in GMR when we compared it with that of spin valves without any inserted layer. The layer of partially oxidized Fe was more effective for improving the properties of the spin valve than the layer of partially oxidized $Co_{90}Fe_{10}$. Considering all the results, we can contribute the significant improvement to the combined effect of the modified local electronic structures at the Fe impurities and theenhanced spin-dependent reflections at the $\alpha-Fe_{2}O_{3} phase in the magnetic layer.

SPIN POLARIZED PHOTOEMISSION AND MAGNETIC CIRCULAY DICHROISM STUDY OF FeAl THIN FILMS

  • Kim, K.W.;Kudryavtsev, Y.V.;Chang, G.S.;Whang, C.N.;Lee, Y.P.
    • Journal of the Korean Vacuum Society
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    • v.6 no.S1
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    • pp.53-58
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    • 1997
  • It is well known that the equiatomic FeAl alloy crystallizes in a paramagnetic CsCl structure and is very stable in a wide temperature range owing to a significant charge transfer from Al to Fe. A presence of structural defects normally enhances the magnetic and magneto-optical properties of this alloy. In this study spin-resolved photoemission and magnetic circular dichroism (MCD) were carried out on both ordered and disordered $Fe_{0.52}Al_{0.48}$ alloy films. The disordered state in the alloy films was obtained by a vapor quenching deposition on cooled substrates. It is shown that the order-disorder transition in the Fe0.52Al0.48 alloy films leads to a significant change in the spin polarization. Form the MCD results the orbital and spin magnetic moments of the constituent atoms are obtained. According to the sum rule the spin and orbital magnetic moments of Fe in the disordered FeAl film are $\mu\frac{SR}{spin}=0.8\mu_B$ and $\mu\frac{SR}{orb}=0.14\mu_B$ respectively. The spin magnetic moment is also evaluated to be $\mu\frac{BR}{spin}=0.77\mu_B$ by the branching ration method employing a photon polarization of 90%.

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Modeling of GMR Isolator for Data Transmission Utilizing Spin Valves (스핀밸브를 이용한 데이터 전송용 GMR 아이솔레이터의 모델링)

  • Park, S.;Kim, J.;Jo, S.
    • Journal of the Korean Magnetics Society
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    • v.14 no.3
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    • pp.109-113
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    • 2004
  • GMR isolator was modeled using a Wheatstone bridge which is profitable for transmitting rectangular wave digital data, and the output voltage characteristics in relation to the input current were investigated in time domain. GMR isolator modeling was divided into two parts, namely magnetic and electric parts. The flow chart of the modeling was drawn in which measured MR curve of the spin valves were incorporated to obtain the electrical voltage output. For magnetic modeling, 3-dimensional model of planar coil was analyzed by FEM method to obtain the magnetic field strength corresponding to the input current. For electric modeling, resistance, inductance and capacitance of the planar coil were calculated and magnetic field waveform was obtained corresponding to the coil current waveform in time domain. Finally, MR-H curves of spin valves and the magnetic field waveform at the spin valves were composited to obtain the output voltage waveform of the isolator. Even though the amplitude of the coil current waveform was increased by 100%, decreased by 90%, or delayed by 10% of the period compared with the input current, similar transmitted output voltage waveform to the input current waveform was obtained due to hysteretic characteristics of the spin valves at the transmission speed of over 400 Mbit/s.

COERCIVE FIELD AND SPIN-GLASS BEHAVIOR OF AMORPHOUS Y-Fe ALLOYS

  • Fujita, A.;Fukamichi, K.
    • Journal of the Korean Magnetics Society
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    • v.5 no.5
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    • pp.762-766
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    • 1995
  • The coercive field $H_{c}$ of amorphous Y-Fe alloys in the spin-glass state has been investigated. Foramorphous $Y_{10}Fe_{90}$ alloy, the thermal variations of $H_{c}$ in the maximum external field $H_{max}=300,\;600$ and 1 k Oe exhibit a maximum. Since spin-glass behavior is strongly affected by external magnetic fields, the maximum point moves to lower temperature with increasing $H_{max}$. The appearance of the maximum in $H_{c}$ has been discussed in terms of the change of the spin-glass state in the external magnetic field. When the value of $H_{max}$ is 55 kOe, the temperature dependence of $H_{c}$ has no maximum and shows an exponential decrease with increasing temperature. Similar trends have been observed over a wide concentration range. The concentration dependence of $H_{c}$ is associated with the magnetic phase diagram.

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Spin-polarized Current Switching of Co/Cu/Py Pac-man type II Spin-valve

  • Lyle, Andrew;Hong, Yang-Ki;Choi, Byoung-Chul;Abo, Gavin;Bae, Seok;Jalli, Jeevan;Lee, Jae-Jin;Park, Mun-Hyoun;Syslo, Ryan
    • Journal of Magnetics
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    • v.15 no.3
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    • pp.103-107
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    • 2010
  • We investigated spin-polarized current switching of Pac-man type II (PM-II) nanoelements in Pac-man shaped nanoscale spin-valves (Co/Cu/Py) using micromagnetic simulations. The effects of slot angle and antiferromagnetic (AFM) layer were simulated to obtain optimum switching in less than 2 ns. At a critical slot angle of $105^{\circ}$, the lowest current density for anti-parallel to parallel (AP-P) switching was observed due to no vortex or antivortex formation during the magnetic reversal process. All other slot angles for AP-P formed a vortex or antivortex during the magnetization reversal process. Additionally, a vortex or anti-vortex formed for all slot angles for parallel to anti-parallel (P-AP) switching. The addition of an AFM layer caused the current density to decrease significantly for AP-P and P-AP at slot angles less than $90^{\circ}$. However, at slot angles greater than $90^{\circ}$, the current density tended to decrease by less amounts or actually increased slightly as shape anisotropy became more dominant. This allowed ultra-fast switching with 5.05 and $5.65{\times}10^8\;A/cm^2$ current densities for AP-P and P-AP, respectively, at a slot angle of $105^{\circ}$.