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Cloning and Characterization of Porcine Uroplakin II Gene

  • D. N. Kwon;H. K. Shin;C. K. Hwang;D. W. Ok;Kim, J. H.
    • Proceedings of the KSAR Conference
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    • 2001.03a
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    • pp.19-19
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    • 2001
  • Mammalian urothelium undergoes unique membrane specialization by making the asymmetric unit membrane (AUM) that is covered with the apical cell surface during terminal differentiation. The AUM contains several major integral membrane proteins including uroplakin Ia, Ib, II and III. The genes for uroplakins have been cloned from humans and mice, but not from porcine. In this study, we report the cloning of the UPII genomic DNA, which codes for the full length open reading frame for the uroplakin II protein. The deduced amino acid sequence encodes of a hydrophobic NH$_2$-terminal peptide, a prosequence, and a mature protein. The prosequence contains three potential N-glycosylation sites and a RGRR cleavage site that may be involved in uroplakin II processing and maturation. Northern and immunohistochemistry analyses showed that the porcine UPII gene is only expressed in urothelium and that the protein was specifically localized in urothelial superficial cells. A 2kb of upstream in the promoter sequence contains multiple transcription factor binding sites, including GC-box, SPI, AP2, and GATA-box sites, but not for TATA or CAAT-box sequences. Comparison of the porcine UPII promoter sequence with that of the murine by MEME system presented two conserved motifs, suggesting a cis-acting regulatory role for the conserved sequences. Sequence homology between two species in motif A and B was 79% and 80% respectively, although their relative locations were different. During the gestation, mouse bladder at estrus stages and day 10 after parturition showed higher UPII expression, while showed lower expression at peri-implantation stage. Taken together, our results showed that the porcine UPII gene was expressed highly and specifically in the bladder urothelium and that steroid hormones for implantation changed the expression of UPII in the bladder, although the biological significance of UPII remains to be not determined.

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Development of drought monitoring system using spatial information big data (공간정보 빅데이터를 활용한 가뭄 모니터링 체계 구축)

  • Won-Ho Nam;Hee-Jin Lee;Chang-Kyun Park;Jong-Hun Kam;Ho-Sun Lee
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.331-331
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    • 2023
  • 일반적으로 가뭄을 해석하기 위하여 가뭄심도, 빈도, 피해면적 및 기간의 영향 등을 고려한 가뭄지수를 이용하며, 이러한 가뭄지수는 주로 지점자료 기반 지상관측자료를 활용하여 산정한다. 하지만 지점자료 특성상 미계측 지역에 대한 정확한 데이터 취득이 어렵기 때문에 미계측 지역에 대한 가뭄 분석의 한계가 발생한다. 다양한 계측기반의 지상센서들이 확충되면서 통계학적 기법기반 공간분포 개선방안을 제시하고 있지만, 정확한 가뭄평가 자료가 추가 및 개선되는 것이 중요하다. 본 연구에서는 원격탐사기술을 활용하여 지점자료의 한계를 극복한 격자기반의 공간정보를 표출함으로써 새로운 가뭄모니터링 방안을 제시하는 것을 목표로 한다. 이를 위해 지상관측자료로 가뭄을 판단하기 어려운 미계측 지역에 대한 가뭄 판단 및 예측 정확도 향상을 위하여 원격탐사기술을 활용한 공간정보 빅데이터를 구축하고자 한다. 미국 국립가뭄경감센터에서 제시한 식생가뭄반응지수 (VegDRI, Vegetation Drought Response Index)는 식생지수, 기상학적 가뭄지수, 지역적 특성을 반영한 생물물리학적 정보를 통합한 하이브리드 가뭄지수로 가뭄과 관련된 공간정보를 활용하여 가뭄을 판단하는 지표이다. VegDRI 산정을 위하여 ERA5의 격자기반 강수자료, MODIS 센서 기반 식생지수 등 격자기반의 공간정보를 수집하였으며, 전처리 모듈을 구축하였다. 또한, 기존 기상학적 가뭄지수인 표준강수지수 (SPI, Standardized Precipitation Index)와 비교를 통해 VegDRI의 국내 적용성을 평가하였으며, 국내 가뭄사례에 적용하여 적절한 가뭄 판단지표로써 활용할 수 있을 것으로 판단된다.

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Evaluation of Utilization of Satellite Remote Sensing Data for Drought Monitoring (가뭄 모니터링을 위한 인공위성 원격탐사자료의 활용 가능성 평가)

  • Won, Jeongeun;Son, Youn-Suk;Lee, Sangho;Kang, Limseok;Kim, Sangdan
    • Korean Journal of Remote Sensing
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    • v.37 no.6_2
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    • pp.1803-1818
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    • 2021
  • As the frequency of drought increases due to climate change, it is very important to have a monitoring system that can accurately determine the situation of widespread drought. However, while ground-based meteorological data has limitations in identifying all the complex droughts in Korea, satellite remote sensing data can be effectively used to identify the spatial characteristics of drought in a wide range of regions and to detect drought. This study attempted to analyze the possibility of using remote sensing data for drought identification in South Korea. In order to monitor various aspects of drought, remote sensing and ground observation data of precipitation and potential evapotranspiration, which are major variables affecting drought, were collected. The evaluation of the applicability of remote sensing data was conducted focusing on the comparison with the observation data. First, to evaluate the applicability and accuracy of remote sensing data, the correlations with observation data were analyzed, and drought indices of various aspects were calculated using precipitation and potential evapotranspiration for meteorological drought monitoring. Then, to evaluate the drought monitoring ability of remote sensing data, the drought reproducibility of the past was confirmed using the drought index. Finally, a high-resolution drought map using remote sensing data was prepared to evaluate the possibility of using remote sensing data for actual drought in South Korea. Through the application of remote sensing data, it was judged that it would be possible to identify and understand various drought conditions occurring in all regions of South Korea, including unmeasured watersheds in the future.

Analysis and Validation of Geo-environmental Susceptibility for Landslide Occurrences Using Frequency Ratio and Evidential Belief Function - A Case for Landslides in Chuncheon in 2013 - (Frequency Ratio와 Evidential Belief Function을 활용한 산사태 유발에 대한 환경지리적 민감성 분석과 검증 - 2013년 춘천 산사태를 중심으로 -)

  • Lee, Won Young;Sung, Hyo Hyun;Ahn, Sejin;Park, Seon Ki
    • Journal of The Geomorphological Association of Korea
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    • v.27 no.1
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    • pp.61-89
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    • 2020
  • The objective of this study is to characterize landslide susceptibility depending on various geo-environmental variables as well as to compare the Frequency Ratio (FR) and Evidential Belief Function (EBF) methods for landslide susceptibility analysis of rainfall-induced landslides. In 2013, a total of 259 landslides occurred in Chuncheon, Gangwon Province, South Korea, due to heavy rainfall events with a total cumulative rainfall of 296~721mm in 106~231 hours duration. Landslides data were mapped with better accuracy using the geographic information system (ArcGIS 10.6 version) based on the historic landslide records in Chuncheon from the National Disaster Management System (NDMS), the 2013 landslide investigation report, orthographic images, and aerial photographs. Then the landslides were randomly split into a testing dataset (70%; 181 landslides) and validation dataset (30%; 78 landslides). First, geo-environmental variables were analyzed by using FR and EBF functions for the full data. The most significant factors related to landslides were altitude (100~200m), slope (15~25°), concave plan curvature, high SPI, young timber age, loose timber density, small timber diameter, artificial forests, coniferous forests, soil depth (50~100cm), very well-drained area, sandy loam soil and so on. Second, the landslide susceptibility index was calculated by using selected geo-environmental variables. The model fit and prediction performance were evaluated using the Receiver Operating Characteristic (ROC) curve and the Area Under Curve (AUC) methods. The AUC values of both model fit and prediction performance were 80.5% and 76.3% for FR and 76.6% and 74.9% for EBF respectively. However, the landslide susceptibility index, with classes of 'very high' and 'high', was detected by 73.1% of landslides in the EBF model rather than the FR model (66.7%). Therefore, the EBF can be a promising method for spatial prediction of landslide occurrence, while the FR is still a powerful method for the landslide susceptibility mapping.

Development of Micro Thermal Image Acquisition System (마이크로 열화상 계측 시스템의 IOT 모듈화 개발)

  • Lee, Jun-Yeob;Oh, Jong-woo;Lee, DongHoon
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2017.04a
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    • pp.169-169
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    • 2017
  • 스마트 돈사 내의 열환경 분석에 필수적으로 고려되어야 인자는 가축의 복사 에너지 변화로 볼 수 있다. 열환경 제어의 대상이기도 하지만 회귀적으로 열환경 변화의 인자이기도 하다. 이러한 가축의 복사 에너지 분석을 위하여 시설 내에 용이하게 배포가 가능한 열화상 계측 시스템을 개발하였다. 초소형 마이크로 열화상 계측 시스템에 부가적으로 IOT(Internet of Thing) 기반 기술을 이용한 모듈화 개발을 병행하였다. 열화상 계측 센서로 LWIR(Longwave infrared)영역에 해당하는 $8{\mu}m{\sim}4{\mu}m$의 영역에서 $0.05^{\circ}C$의 분해능을 보이는 $Lepton^{TM}$ (500-0690-00, FLIR, Goleta, CA)모델을 사용하였다. SPI(Serial Peripheral Interface) 속도 2 Mhz로 마이크로프로세서(NanoPi NEO Air, FrendlyArm, CA, USA)와 고속 통신을 수행하여 9 Hz의 계측이 가능하다. 열화상 센서와 마이컴으로 구성되는 단위 계측 시스템의 통신 기능 확장을 위하여 다음과 같이 세 단계의 정보 전달 시나리오를 설계하였다. 1) 단독적으로 열화상을 계측 하고 내장된 메모리에 저장하는 형식 2) 인접한 사용자 인터페이스에서 1번 단독 모듈에 접속하여 열화상을 실시간으로 전송하여 화면에 도시하는 형식 3) 2번 사용자 도시모듈과 병행적으로 Local WI-FI 통신을 이용한 모바일 기기에 화면을 도시하는 형식. 이와 같은 계층적이며 모듈화된 계측 시스템을 구성하기 위해서 1번 모듈에 공개 소프트웨어인 Hostapd 2.5(http://w1.fi/hostapd)버전을 설치하였다. 외부 인터넷 환경이 없는 상황에 1번 모듈 단독으로 AP(Access Point) 기능을 제공하여 지근 거리에 있는 2번 모듈과 3번 모바일 기기의 접속을 관리할 수 있다. 2번 모듈의 경우 화면 다수의 1번 모듈에 접속을 교차적으로 수행하는 방식과 2번 모듈 자체가 AP가 되어 1번 모듈의 접속을 허용하는 형태로 구성되어 있다. 계측 시스템의 계측 매트릭스 구성에 따라 선택적으로 결정할 수 있다. 1번 2번 모듈 공통적으로 TCP/IP Listener와 Client 서비스를 병렬적으로 수행할 수 있도록 개발을 하였다. 3번 모바일 기기에서 사용자 인터페이스 구현을 위하여 범용 Android 기반 GUI 프로그램과 Socket 통신을 연동시켰다. 1개의 열화상 Frame의 전송량은 9,600 Byte ($=80{\times}60{\times}2Byte$) 로 WI-FI 통신 전송 시 2회 ~ 6회 정도 내외로 가변적인 통신 수행 횟수를 나타내었다. 센서 계측 시스템과 정보 전송 시스템을 병렬적으로 구성한 모듈화 된 계측시스템의 전 요소에서 센서에서 제공하는 최대 계측 주기인 9 Hz 구현이 일반적으로 가능하였다. 이를 이용한 추후 연구를 통해 가축 객체의 열복사 정보와 돈사 내 열환경 간의 역학성을 연구할 것이다.

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Assessment of CMIP5 GCMs for future extreme drought analysis (미래 극한 가뭄 전망을 위한 CMIP5 GCMs 평가)

  • Hong, Hyun-Pyo;Park, Seo-Yeon;Kim, Tae-Woong;Lee, Joo-Heon
    • Journal of Korea Water Resources Association
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    • v.51 no.7
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    • pp.617-627
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    • 2018
  • In this study, CMIP5 GCMs rainfall data (2011~2099) based on RCP scenarios were used to analyze the extreme drought evaluation for the future period. For prospective drought assessment, historical observations were used based on the Automated Surface Observing System (ASOS) data (1976~2010) of the Korea Meteorological Administration. Through the analysis of various indicators, such as average annual rainfall, rainy days, drought spell, and average drought severity was carried out for the drought evaluation of the five major river basins (Han river, Nakdong river, Geum river, Sumjin river, and Youngsan river) over the Korean peninsula. The GCMs that predicted the most severe future droughts are CMCC-CMS, IPSL-CM5A-LR and IPSL-CM5A-MR. Moderate future droughts were predicted from HadGEM2-CC, CMCC-CM and HadGEM2-ES. GCMs with relatively weak future drought forecasts were selected as CESM1-CAM5, MIROC-ESM-CHEM and CanESM2. The results of this study might be used as a fundamental data to choose a reasonable climate change scenario in future extreme drought evaluation.

Studies on the Physiological Chemistry of the Spring Growth Habits in Naked Barley V. Changes in the Isozyme Patterns and Activities of Peroxidase During the Differentiation (과맥의 파성에 대한 생리화학적 연구 V. 유수의 분화, 발육과정중 Peroxidase의 활성 및 Isozyme Pattern)

  • 최선영;이강수;박기훈
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.3
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    • pp.375-382
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    • 1986
  • This study was carried out to obtain the basic information for the clarification of spring growth habits mechanism of naked barleys. The isozyme patterns and activities of peroxidase in the young spike and leaf blade were analyzed during the differentiation and development of young spike. The characteristic differences between the normal and rosetted type were in c and g isozymes in young spike, and in i isozyme in the leaf blade. In the normal type, c and i isozymes disappeared at the stage of spi-kelet differentiation, g isozyme at the stage of flolet differentiation. But, in the rosetted type, those three isozymes remained in dark stained condition until the time of final sampling. Especially, those three isozymes were higher in the rosetted type than those in the normal type even at the stage of bract differentiation(BDS), just prior to the reproductive stage. The activities of peroxidase decreased slowly after BDS in the young spike and leaf blade in the normal type, While, in the rosetted type, increased linearly, and the degree of increasing was remarkable in the young spike. It was interesting that the degree of activities in young spike was higher in the rosetted type than that in the normal type even at BDS. From the above results, the remarkable differences of the isozyme patterns and activities at BDS between the normal and rosetted type were considered to be the physiological expression of the varieties concerned with the degree of spring growth habits.

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