• 한국식물학회:학술대회논문집
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• 한국식물학회 2002년도 춘계학술발표대회:발표눈문요지록
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• pp.73-82
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• 2002

The committing step in Met and S-adenosyl-L-methionine (SAM) synthesis is catalyzed by cystathionine ${\gamma}$ -synthase (CGS). Transgenic Arabidopsis thaliana overexpressing CGS under control of 35S promoter show increased soluble Met and its metabolite S-methylmethionine, but only at specific stages of development. CGS-overexpressing seedlings are resistant to ethionine. Similar results were obtained with transgenic potato plants overexpressing Arabidopsis CGS. Several of the transgenic lines show silencing of CGS resulting in deformed p]ants with a reduced capacity for reproductive growth similar as transgenic plants by antisense RNA (CGS[-]). Exogenous feeding of Met to the CGS[-] and CGS[+] silenced plants partially restores their growth. Similar morphological deformities are observed in plants cosuppressed for SAM synthetase, even though such plants accumulate 250 fold more soluble Met than wild type and they overexpress CGS. The results suggest that the abnormalities associated with CGS and SAM synthetase silencing are due in part to a reduced ability to produce SAM, and that SAM may be a regulator of CGS expression.

• 아시안잔디학회지
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• 제18권4호
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• pp.211-219
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• 2004

Transgenic zoysiagrass (Zoysia japonica cv. Zenith) plants have been obtained by particle bombardment of embryogenic callus with the plasmid pSMABuba, which contains hygromycin resistance (hpt) and bialaphos resistance (bar) genes. Parameters on DNA delivery efficiency of the particle bombardment were partially optimized using transient expression assay of a chimeric $\beta-glucuronidase$(gusA) gene driven by the CaMV 35S promoter. Stably transfarmed zoysiagrass plants were recovered with a selection scheme using hygromycin. Transgenic zoysiagrass plants were confirmed by PCR analysis with specific primer for bar gene. Expression of the transgene in transformed zoysiagrass plants was demonstrated by Reverse transcriptase (RT)-PCR analysis. All the tested transgenic plants showed herbicide BastaR resistance at the field application rate of $0.1\%-0.3\%$.

• 환경생물
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• 제28권3호
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• pp.158-171
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• 2010

비교적 크기가 크고 남방계인자들이 간헐적으로 보고 된 덕적도 일대에 대하여 자연자원의 분포를 규명하고, 환경 및 기후변화 등의 기초 자료를 제공하기 위하여 관속식물에 대한 분포조사를 2007년 9월부터 2008년 9월까지 35회에 걸쳐 수행하였다. 본 조사를 통해 확인된 조사지역 내의 소산 관속식물은 108과 320속 480종 2아종 45변종 8품종의 535분류군으로 확인되었다. 관속식물 중에서 국화과 식물이 가장 많은 비율을 차지하고 있었고 벼과, 콩과, 장미과의 순이었다. 특정식물로는 한국 특산 관속식물 4분류군, 산림청 지정 희귀 및 멸종위기식물 6분류군, 환경부지정 특정식물종 55분류군이 각각 조사되었고, 반면에 귀화식물은 50분류군이 조사되어 전체식물상의 약 17.9%를 차지하였다. 조사지역의 식생은 일부 지역에서 인간 활동에 의해 교란되어 있었으나 대체로 양호한 상태이고 일부 지역은 특이할 만한 식물들도 발견되어 체계적인 보호가 요구되어졌다. 인간 간섭이 심할 것으로 판단되는 저지대의 대부분 지역에서 귀화식물의 점유율이 아주 높았고, 차후로 귀화식물의 분포가 빠르게 확산될 것이 예상되어 생태계의 교란이 우려되어 지므로 이에 대한 대책이 수립되어야 할 것으 로 보아진다.

• Molecules and Cells
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• 제28권5호
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• pp.463-472
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• 2009

Although the possible cellular roles of several ubiquitin-specific proteases (UBPs) were identified in Arabidopsis, almost nothing is known about UBP homologs in rice, a monocot model plant. In this report, we searched the rice genome database (http://signal.salk.edu/cgi-bin/RiceGE) and identified 21 putative UBP family members (OsUBPs) in the rice genome. These OsUBP genes each contain a ubiquitin carboxyl-terminal hydrolase (UCH) domain with highly conserved Cys and His boxes and were subdivided into 9 groups based on their sequence identities and domain structures. RT-PCR analysis indicated that rice OsUBP genes are expressed at varying degrees in different rice tissues. We isolated a full-length cDNA clone for OsUBP6, which possesses not only a UCH domain, but also an N-terminal ubiquitin motif. Bacterially expressed OsUBP6 was capable of dismantling K48-linked tetra-ubiquitin chains in vitro. Quantitative real-time RT-PCR indicated that OsUBP6 is constitutively expressed in different tissues of rice plants. An in vivo targeting experiment showed that OsUBP6 is predominantly localized to the nucleus in onion epidermal cells. We also examined how knock-out of OsUBP6 affects developmental growth of rice plants. Although homozygous T3 osubp6 T-DNA insertion mutant seedlings displayed slower growth relative to wild type seedlings, mature mutant plants appeared to be normal. These results raise the possibility that loss of OsUBP6 is functionally compensated for by an as-yet unknown OsUBP homolog during later stages of development in rice plants.

• The Plant Pathology Journal
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• 제38권3호
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• pp.229-238
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• 2022

Fusarium wilt caused by Fusarium oxysporum f. sp. niveum (Fon) is the most serious soil-borne disease in the world and has become the main limiting factor of watermelon production. Reliable and quick detection and quantification of Fon are essential in the early stages of infection for control of watermelon Fusarium wilt. Traditional detection and identification tests are laborious and cannot efficiently quantify Fon isolates. In this work, a real-time polymerase chain reaction (PCR) assay has been described to accurately identify and quantify Fon in watermelon plants and soil. The FONRT-18 specific primer set which was designed based on identified specific sequence amplified a specific 172 bp band from Fon and no amplification from the other formae speciales of Fusarium oxysporum tested. The detection limits with primers were 1.26 pg/µl genomic DNA of Fon, 0.2 pg/ng total plant DNA in inoculated plant, and 50 conidia/g soil. The PCR assay could also evaluate the relationships between the disease index and Fon DNA quantity in watermelon plants and soil. The assay was further used to estimate the Fon content in soil after disinfection with CaCN₂. The real-time PCR method is rapid, accurate and reliable for monitoring and quantification analysis of Fon in watermelon plants and soil. It can be applied to the study of disease diagnosis, plant-pathogen interactions, and effective management.

• 건축역사연구
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• 제26권6호
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• pp.29-40
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• 2017

Soswaewon as a typical villa garden of Korea is a symbolic garden that its diverse compositions imply a specific meaning. This study reviewed the purpose of introduction, meaning, and location of the landscape plants as one of the compositions and covering quite a large part of Soswaewon through 48 Yeong (meaning 48 poems of Soswaewon) and Soswaewondo (meaning Map of Soswaewon). In 48 Yeong, 27 verses describe landscape plants as their key poetic matters. The most frequently mentioned top 3 plants are bamboos, pine trees, and Japanese apricots. The three plants are called Sehansamwoo(歲寒三友) showing constant fidelity and loyalty in any changing situations, which was regarded to represent the nature of scholars. And also the study examined the frequency and planting location of the plants in Soswaewon by comparing 48 Yeong and Soswaewondo, and the result showed some differences between them. That seems to be caused by the limitations in the comparison, because in case of 48 Yeong, the location and frequency of landscape plants can be examined only through the context of the verses, and Soswaewondo showed different production time from 48 Yeong and expressed them only in a form of drawing. The plants have symbolic meanings multi-layered and ambiguous. With their symbolic meanings, the landscape plants reviewed through 48 Yeong consistently represent fidelity and loyalty, man of virtue, and hermit. That is, Soswaewon is the garden granting some significance to its compositions by interacting with the things.

• 한국자원식물학회지
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• 제28권4호
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• pp.511-525
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• 2015

The purpose of this study is to offer the flora data for conserving the biodiversity of Gyeongju National Park by objective surveying and analysing the flora distributed in Mt. Gumi District, Gyeongju National Park, South Korea. The flora of Mt. Gumi were surmmarized as 476 taxa including 97 families, 297 genera, 419 species, 3 subspecies, 46 varieties and 8 forms. The rare plants were 6 taxa such as Aristolochia contorta, Paeonia lactiflora var. trichocarpa, Potentilla discolor, Berchemia berchemiaefolia, Trigonotis icumae and Iris odaesanensis. The Korean endemic plants were 9 taxa such as Carpinus laxiflora, Pseudostellaria coreana, Philadelphus schrenkii, Indigofera koreana, Lespedeza maritima, Vicia chosenensis, Galium koreanum, Lonicera subsessilis and Weigela subsessilis. The specific plants by floristic region were 38 taxa such as Salix chaenomeloides(I), Hylomecon vernalis (I), Oxalis obtriangulata (I), Viola orientalis(II), Adoxa moschatellina (II), Cirsium chanroenicum (II), Vitex negundo var. incisa (III) and so forth. The naturalized plants were 36 taxa such as Fallopia dumetorum, Chenopodium album, Lepidium virginicum, Amorpha fruticosa, Euphorbia supina, Carduus crispus, Erigeron strigosus, Festuca myuros and so forth. The invasive aline plant was Aster pilosus. The target plants adaptable to climate change were 8 taxa such as Artemisia rubripes, Carpesium macrocephalum and so forth.

• 한국자원식물학회지
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• 제20권1호
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• pp.28-37
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• 2007

본 연구는 남해안 무인도서 가운데 목도, 곡두도, 대항도, 불근도의 관속식물과 식생 현황 및 식물구계학적 특정식물을 조사함으로써, 현재 관리와 이용 실태에 따른 식물 변화를 예측하고 차후 무인도서의 식물 보전을 위한 기초 자료의 일환으로 수행하였다. 전체 관속식물은 80과 193속 234종 32변종 5품종 1교잡종으로 총 272종류(taxa)가 확인되었으며, 귀화식물과 귀화율은 12종류와 4.4%로 분석되었다. 또한, 식물구계학적 특정식물종은 IV등급에 4분류군이, III등급에는 10분류군이, II등급에는 5분류군이, I등급에는 35분류군의 총 54종류가확인되었으나 V등급에 해당되는 특정식물은 출현하지 않았다. 목도에서는 곰솔이 우점하고 있으며, 일본특산식물로 알려져 왔던 부추속(Allium)의 갯부추(Allium pseudojaponicum)가 30개체 이상이 분포하고 있는 것으로 확인되었다. 곡두도는 밀사초(Carex boottiana)가 큰 군락을 형성하고 있었으며, 대항도는 우리나라에서도 큰 면적 중 하나일 것으로 추측되는 후박 나무군락이 전 지역에서 분포하고 있었다. 또한, 불근도에서는 2003년도 국립공원관리공단에서 참식나무와 후박나무 등 수백개체를 식재하였으나 과거에 방목된 토끼로 인해 많은 개체가 고사한 상태로 방치되고 있다.

• Applied Biological Chemistry
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• 제37권4호
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• pp.248-254
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• 1994

Bromoxynil은 쌍떡잎 특이적 제초제로써 폭 넓게 이용되고 있으며 반감기가 매우 짧다. Bromoxynil을 3,5-dibromo-4-hydroxybenzoic acid로 분해하는 nitrilase를 암호화한 bxn 유전자를 식물 벡터인 pGA482에 도입하고 Agrobacterium과의 동시배양을 통해 담배와 상추에 형질전환하였다. Kanamycin을 이용해 형질전환 식물체를 선별하고 완전한 식물체로 분화시켰다. Northern hybridization을 통해 bxn 유전자의 발현정도를 검정하고 liaf-disc와 pot assay를 통해 형질전환 식물체가 고농도의 bromoxynil에 저항성을 보임을 확인하였다.

• 한국환경복원기술학회지
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• 제12권4호
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• pp.47-71
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• 2009

2007년 4월부터 2007년 10월까지 가평군 북부지역의 자원식물분포조사를 수행하였다. 본 조사를 통해 확인된 조사지역 내의 소산 관속식물은 94과 286속 412종 4아종 52변종 12품종 등 총 480분류군으로 확인되었다. 조사 지역에서 분포하고 있는 480종류의 식물자원 유용도를 분석한 결과 식용자원식물이 177종류, 섬유용 자원식물이 6종류, 약용 자원식물이 124종류, 관상용 자원식물이 133종류, 초지 자원식물이 136종류, 공업원료자원이 2종류, 염료용 자원식물이 69종류, 목재용 자원식물이 10종류, 녹화식물이 24종류, 용도가 밝혀지지 않은 식물이 72종류로 구분되었다. 이 중 식용자원, 약용자원, 초지자원, 관상용자원이 비교적 많이 분포하는 것으로 나타났으며, 이는 전체식물의 82.3%를 차지하였다. 특기할만한 식물로는 한국 특산 관속식물 14분류군, 환경부 지정 특정식물종 85분류군, 산림청지정 희귀 및 멸종위기식물 12분류군이 각각 조사되었고, 귀화식물은 22분류군으로서 약 4.6%를 차지하였고, 도시화지수는 8.2%로 조사되었다. 조사지역의 식생은 비교적 양호한 편이었으나, 현재 가평군에 위치한 여러 산들이 등산객을 위한 탐방로 개발 및 확장과 임도의 발달로 인해 귀화율은 점차 증가할 것으로 사료된다.