• The Plant Pathology Journal
• /
• v.35 no.2
• /
• pp.112-124
• /
• 2019

Barley worldwide is affected seriously by Fusarium seedling blight (FSB) and Fusarium head blight (FHB) diseases caused by the Fusarium species. The objective of this study was to facilitate the resistance of hulled and hull-less barley at different growth stages to F. culmorum according to direct parameters: disease rating (DR), fresh weight of leaves and roots, kernel weight per spike, kernel number per spike, plump kernels, and indirect parameters - chlorophyll a fluorescence (CF). Plate assay, greenhouse and field tests were performed on 30 spring barley doubled haploid (DH) lines and their parents infected with Fusarium culmorum. Direct parameters proved that hulled genotypes show less symptoms. Most studied chlorophyll a fluorescence (CF) parameters (apart from DIo/CS - amount of energy dissipated from PSII for laboratory test, TRo/CS - amount of excitation energy trapped in PSII reaction centers, ETo/CS - amount of energy used for electron transport and RC/CS - number of active reaction centres in the state of fully reduced PSII reaction center in field experiment) were significantly affected by F. culmorum infection. In all experiments, hulled genotypes had higher values of CF parameters compared to hull-less ones. Significant correlations were detected between direct and indirect parameters and also between various environments. It was revealed that ABS/CS, TRo/CS, and RC/CS have significant positive correlation in greenhouse test and field experiment. Significant correlations suggest the possibility of applying the CF parameters in selection of barley DH lines resistant to F. culmorum infection.

• Clinical and Experimental Reproductive Medicine
• /
• v.46 no.1
• /
• pp.14-21
• /
• 2019

Objective: The usual seminal profile has been customarily used for diagnosing male infertility based on an examination of semen samples. However, sperm DNA fragmentation has also been causally linked to reproductive failure, suggesting that it should be evaluated as part of male infertility assessments. To compare the ability of the five most widely utilized methodologies of measuring DNA fragmentation to predict male infertility and reactive oxygen species by Oxisperm kit assay. Methods: In this case-control study, which received ethical committee approval, the participants were divided into fertile and infertile groups (50 patients in each group). Results: The alkaline comet test showed the best ability to predict male infertility, followed by the terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay, the sperm chromatin dispersion (SCD) test, and the sperm chromatin structure assay (SCSA), while the neutral comet test had no predictive power. For our patient population, the projected cut-off point for the DNA fragmentation index was 22.08% using the TUNEL assay, 19.90% using SCSA, 24.74% using the SCD test, 48.47% using the alkaline comet test, and 36.37% using the neutral comet test. Significant correlations were found between the results of the SCD test and those obtained using SCSA and TUNEL (r = 0.70 and r = 0.68, respectively; p< 0.001), and a statistically significant correlation was also found between the results of SCSA and the TUNEL assay (r = 0.77, p< 0.001). Likewise, the results of the alkaline comet test showed significant correlations with those of the SCD, SCSA, and TUNEL tests (r = 0.59, r = 0.57, and r = 0.72, respectively; p< 0.001). Conclusion: The TUNEL assay, SCSA, SCD, and the alkaline comet test were effective for distinguishing between fertile and infertile patients, and the alkaline comet test was the best predictor of male infertility.

• Animal Bioscience
• /
• v.37 no.8
• /
• pp.1355-1366
• /
• 2024

Objective: The analysis of runs of homozygosity (ROH) has been applied to assess the level of inbreeding and identify selection signatures in various livestock species. The objectives of this study were to characterize the ROH pattern, estimate the rate of inbreeding, and identify signatures of selection in the red-brown Korean native chickens. Methods: The Illumina 60K single nucleotide polymorphism chip data of 651 chickens was used in the analysis. Runs of homozygosity were analysed using the PLINK v1.9 software. Inbreeding coefficients were estimated using the GCTA software and their correlations were examined. Genomic regions with high levels of ROH were explored to identify selection signatures. Results: A total of 32,176 ROH segments were detected in this study. The majority of the ROH segments were shorter than 4 Mb. The average ROH inbreeding coefficients (F_ROH) varied with the length of ROH segments. The means of inbreeding coefficients calculated from different methods were also variable. The correlations between different inbreeding coefficients were positive and highly variable (r = 0.18-1). Five ROH islands harbouring important quantitative trait loci were identified. Conclusion: This study assessed the level of inbreeding and patterns of homozygosity in Red-brown native Korean chickens. The results of this study suggest that the level of recent inbreeding is low which indicates substantial progress in the conservation of red-brown Korean native chickens. Additionally, Candidate genomic regions associated with important production traits were detected in homozygous regions.

• Ocean and Polar Research
• /
• v.28 no.2
• /
• pp.79-94
• /
• 2006

Spatio-temporal patterns in the zooplankton communities, physicochemical environmental factors, and dominant species were examined in Camak Bay from April, July, September, and November, 2003. A total of 40 taxa were sampled with a mean abundance of total zooplankton ranging from $270{\sim}1,803inds/m^3$. The most abundant species was Acartia erythraea, followed by Eurytemora pacifica, Podon polyphemoides, A. omorii, and Paracalanus parvus s. 1 during the study period. Multivariate analysis revealed significant differences in community structure among three regions: the middle part of the sampling area (B), sites 1, 2 (A) and other sites (C). The number of species and diversity (H') varied significantly among the three regions (p<0.05), but no differences in the mean abundances were observed (p>0.05). In order to investigate factors affecting the correlations between the abundance of dominant species, all zooplankton and environmental factors ware calculated using a correlation analysis. Of these, the abundance of dominant taxon showed positive correlation with seawater temperature, salinity and dissolved oxygen (p<0.05). In statistical analysis showed that the environmental factors influencing the variation of the communities ere predominantly temperature, salinity, and dissolved oxygen.

• Journal of Korean Society for Atmospheric Environment
• /
• v.17 no.5
• /
• pp.395-405
• /
• 2001

To make an assessment of the compatibility between DOAS and conventional point monitoring system (MCSAM-2: MS2), we investigated the concentrations of three criteria pollutants which include S $O_2$, N $O_2$, and $O_3$from a national monitoring station in Seoul during the periods of June 1999~August 2000. The average concentration values for the whole study period derived from hourly concentration data sets of those three species indicated that the mean differences between the two methods can be approximated as 18%. When the bias structure of two systems was evaluated through the computation of percent difference(PD) between the two such as ( $C_{DOAS}$- $C_{conventional}$ $C_{DOAS}$*100, differences between the two systems appeared to be quite systematic among different compounds. While the mode of bias peaked at 0~20% or 20~40% in terms of PD values, the cause of such positive bias mainly arised from generally enhanced concentration values of DOAS system. The structure of bias among different species was further assessed through linear regression analysis. Results of the analysis indicated that the dominant portions of differences observed from two monitoring systems can be accounted for by the systematic differences in their spanning and zeroing systems. S $O_2$(MS2)=0.6385 S $O_2$(DOAS)+2.0985($r^2$=0.7894) N $O_2$(MS2)=0.6548 N $O_2$(DOAS)+7.437($r^2$=0.7687) $O_3$(MS2)=1.0359 $O_3$(DOAS)-7.7885($r^2$=0.7944) The findings of slope values at around 0.64~0.65 from two species suggest that DOAS should respond more sensitively in upper bound concentration range. The offset values apart from zero indicate that more deliberate comparison needs to be made between these monitoring systems. However, based on the existence of strong correlations from at least 8,000 data points for each species of comparison, we were able to conclude that the compatibility of two monitoring systems is highly significant. With the improvement of calibration techniques for the DOAS system. its applicability for routine monitoring of airborne pollutant species is expected to be quite extendable.

• Ocean and Polar Research
• /
• v.27 no.1
• /
• pp.15-24
• /
• 2005

In order to investigate the structures and dynamics of phytoplankton communities, each physicochemical environmental factor, species composition, standing crop, and dominant species were examined in the marine ranching ground of Tongyeong coastal waters from April to October, 2000. During the studies, mean water temperature and salinity were $18.8^{\circ}C$ and 33.1 psu, respectively. DO, SS and transparency varied from 5.43 to 11.39 mg/l, 14.6 to 32.4mg/l and 3.5 to 9.0m, respectively. Light intensities varied from 0.02 to $966{\mu}E/m^2/s$, which decreased with depth. $NH_4-N,\;NO_3-N,\;NO_2-N,\;PO_4-P,\;and\;SiO_2-Si$ were fluctuated from 0.059 to 0.332 mg/l, 0.040 to 0.800 mg/l, 0.001 to 0.468 mg/l, 2.3 to $143.0{\mu}g/l$, and 0.007 to 0.600 mg/l, respectively. chlorophyll a concentrations were fluctuated from 0.7 to $8.9{\mu}g/l$. Among 130 taxa of phytoplankton communities observed. diatoms occupied more than 81.54% of the total species, and the others were dinoflagellates and silicoflagellates. Phytoplankton standing crops ranged from $4.6{\times}10^4\;to\;2.6{\times}10^6cells/l$. In October, the standing crops were at bloom level showing more than $10^6cells/lat$ all stations. Dominant species changed by month and station. Leptocylindrus danicus occupied 59.84% in April and 22.03% in June. Pseudo-nitzschia pungens in August and Chaetoceros socialis and Skeletonema costatum in October were predominant species. In order to investigate factors influencing the total phytoplankton standing crops the correlations between the standing crops of diatoms, dinoflagellates, all phytoplanktons occurred and environmental factors were calculated using a multiple regression analysis. The coefficient of determination $(R^2)$ for total standing crops was 0.63 which explained 63% of variance and that of $R^2$ for diatom was 0.82. In statistical analysis, the results showed that the environmental factors influencing the size of the communities were predominantly water temperature, salinity and silicate.

• Journal of Korean Society of Forest Science
• /
• v.106 no.2
• /
• pp.169-185
• /
• 2017

The result of forest vegetation classification could be quite different and dependant on analysis methods. The purpose of this study was to compare the analyzed results for three kinds of methods (physiognomy dominant species, floristic composition and interspecific association) related to vegetation classification. Vegetation data were collected by the 80 quadrates in Mt. Oseo, Chungcheongnam-do from September to October in 2016. We carried out community type classification using above three methods. As a result, the vegetation according to physiognomy dominant species was classified into ten communities such as Pinus densiflora community, Quercus mongolica community, Zelkova serrata community, Quercus acutissima community, Cornus controversa community, Quercus serrata community, Larix kaempferi community, Pinus rigida community, Castanea crenata community and Liriodendron tulipifera community. The vegetation according to floristic composition was classified into 4 vegetation units. It was totally represented by Lindera erythrocarpa community group. And L. erythrocarpa community group was classified into the Rhododendron mucronulatum community (subdivided R. mucronulatum typical group and Styrax obassia group) and Zelkova serrata community (subdivided Larix kaempferi group and Pseudostellaria palibiniana group). As a result of interspecific association, forest vegetation was divided into two groups. And it was considered that the vegetation type by floristic composition and interspecific association significant could be affected by topography. There were lots of vegetation groups or units in the order like 10 types of communities by the physiognomy dominant species, 8 species group and 4 vegetation types by the floristic composition, and 2 types by the interspecific association. In conclusion, vegetation classification methods elicited diverse vegetation groups or units with lots of correlations of environmental factors.

• Asian-Australasian Journal of Animal Sciences
• /
• v.29 no.1
• /
• pp.73-79
• /
• 2016

The objective was to evaluate the effect of fodder tree species (FTS) with condensed tannin contents: Cordia elaeagnoides, Platymiscium lasiocarpum, Vitex mollis, and Haematoxylon brasiletto, on in vitro methane ($CH_4$) production at 24 h post incubation. The analysis was performed using the in vitro gas production technique, with three levels of inclusion/species: 600, 800, and 1,000 mg and with 4 replicates/species/level of inclusion. The substrate was incubated at $39^{\circ}C$, and the gas and $CH_4$ production were recorded at 4, 8, 12, and 24 h post incubation. The data collected was analyzed through Pearson correlation, polinomial regression and fixed effects models. There were negative correlations between FTS-total gas volume (r = -0.40; p<0.001); FTS-volume of $CH_4$ produced (r = -0.40; p<0.001) and between the inclusion level-volume of $CH_4$ produced (r = -0.20; p<0.001). As well as a positive correlation between hours post incubation-total gas volume (r = 0.42; p<0.001) and between hours post incubation-volume of $CH_4$ produced (r = 0.48; p<0.001). The FTS: C. elaeagnoides, V. mollis, and H. brasiletto have potential, in the three inclusion levels analyzed, to reduce $CH_4$ emission on in vitro trials (>32.7%), taking into account the total $CH_4$ production at 24 h of the forage used as reference (Avena sativa). It's suggested that C. elaeagnoides-according to its crude protein, neutral detergent fiber, and condensed tannins content- is the best alternative within the FTS analyzed, for feeding ruminants and for the control of $CH_4$ emissions during the dry season.

• Journal of Korean Society of Forest Science
• /
• v.87 no.3
• /
• pp.459-465
• /
• 1998

To obtain the basic data for the conservation and cultivation of native herb species under the forest, the relations between distribution of native herb species and site factors, distribution of native herb species, and site factors were surveyed on natural forest at subalpine zone in Mt. Odae. Ainsliaea acerifolia, Pseudostellaria palibiniana and Pimpinedla brachycarpa were distributed mainly on broadleaved mixed forest, Clematis fusca var. coreana, Carex siderosticta. Isodon excisus and Vicia unijuga did mainly on gab sites. The distribution of Ligularia fisheri, Aconitum pseudo-laeve var. erectum, Cacalia hastata and Artemisia stolonifera were significantly differed between slope directions. Artemisia stolonifera was distributed more frequently in south directed slope, and Ligularia fisheri, Aconitum pseudo-laeve var. erectum and Cacalia hastata were distributed more frequently in north and west directed slope than the other. The distribution of Aconitum pseudo-laeve var. erectum and Artemisia stolonifera were significantly differed between soil moisture conditions. Artemisia stolonifera was distributed mainly on the dry site, but Aconitum pseudo-laeve var. erectum did mainly on the dry site. Negative correlations between density of Clematis fusca var. coreana, Carex siderosticta. Isodon excises and Vicia unijuga and crown coverage was proved. Aconitum pseudo-laeve var. erectum, Pimpinella brachycarpa, Meehania urticifolia, Smilacina japonica and Pseudostellaria palibiniana were mainly distributed on wet and shady sites under forest canopy, but Pedicularis resupinata, Clematis fusca var. coreana and Artemisia stolonifera did on sunny sites at forest edge or gab.

• Korean Journal of Ecology and Environment
• /
• v.50 no.4
• /
• pp.403-410
• /
• 2017

We collected fishes at the 12,873 sites in stream order (1~7) from 2008 to 2016. In the results, two populations(Zacco platypus and Zacco koreanus) represented distributional differences in the stream order gradient, and correlation analysis showed that the two populations had a potential competitive relationship. The ecological characteristics of the fish except for the Z. platypus and the Z. koreanus, according to the gradient of stream order showed a pronounced gradient in the species such as intermediate species, sensitive species, carnivores, herbivores and omnivores. The two populations showed a high correlation between intermediate species, sensitive species of the tolerance guild and omnivores, insectivores of the trophic guild. Fish assessment index (FAI) was negative relation with stream order. According to the stream order gradient, the two populations showed different correlations with FAI.