• Journal of Microbiology and Biotechnology
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• 제9권6호
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• pp.820-825
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• 1999

A bacterial strain WN9, which produced a new type of extracellular polysaccharide, was isolated from soil samples. By morphological, physiological, biochemical, and phylogenetic studies, strain WN9 was identified as a Paenibacillus sp. and it was named as Paenibacillus sp. WN9, which produced a high molecular extracellular polysaccharide from glucose. The molecular weight of the exopolysaccharide (EPS-WN9) was estimated to be about 31.5 mega-Da. The FT-IR spectrum of EPS-WN9 revealed typical characteristics of polysaccharides. EPS- WN9 consisted of D-glucose and D-mannose with a molar ratio of 1:1.4 being identified as a neutral sugar component. The acidic component of EPS- WN9 was tyrosine. Rheological analysis of EPS- WN9 revealed that the pseudoplastic property and its apparent viscosity remained stable at various temperatures and pHs.

• 한국유기농업학회지
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• 제7권1호
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• pp.129-135
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• 1998

암면경, 펄라이트경 및 코코피트경 토마토의 생장촉진에 미치는 근권세균류의 영향을 구명하기 위하여 $Azospirilham\;sp.(4.5{\times}10^7cells/g),\;Rhodopseudomonas\;sp.(5.8{\times}10^5cells/g),\;Pseudomonas\;sp.(6.1{\times}10^6cells/g$) 및 Bacillus sp. 와 Corynebacterium glutamicum의 융합체($9.1{\times}10^5cells/g$) 등의 군주를 근권에 처리하였다. 군주의 근권처리시 토마토의 전반적인 생장이 대조구에 비해서 촉진되었으며, 식물생장촉진효과가 가장 우수한 군주는 Azospirillum sp.였다. 군주에 의한 식물생장 촉진 효과가 높은 재배방식 및 배지는 코코피트경 > 필라이트경 = 암면경 등의 순이었다.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2003년도 가을 학술발표회 발표논문집
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• pp.245-247
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• 2003

축산폐수에서 분리된 세균 S. sp1(S. capitis)와 S. sp2(3. xylosus)를 이용한 축산폐수 중 질소화합물 분해정도를 파악한 결과 1) 증식에 알맞은 조건은 LB broth, $27^{\circ}C$, pH7로 나타났다. 2) S. sp1은 배양 7일 초기 농도의 90.3%, 7일째 32.9%로 감소되어 14일까지 다소 증가하면서 유지되었다. 3) S. sp1과 S. sp2를 혼합배양한 결과 S. sp1과 S. sp2 단독배양보다 분해 효율이 높고 기간도 2일정도 단축되었다.

• Journal of Species Research
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• 제9권1호
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• pp.56-67
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• 2020

Eight new species of two genera Dysidea and Euryspongia (Demospongiae: Dictyoceratida: Dysideidae) are described from Gageodo, Ulleungdo, Geomundo, and Jejudo Islands, Korea. Four new species of the genus Dysidea are differentiated by the fibre structure, cored detritus, and fibre arrangement. Dysidea mureungensis n. sp. is characterized by the honeycomb shape of surface and no distinction between primary and secondary fibres. Dysidea glavea n. sp. differs by large sands cored in fibres and that the membrane easily separates from fibres. Dysidea geomunensis n. sp. has fibres that are thinner than those of D. glavea n. sp.. Dysidea corallina n. sp. is characterized by folded fan shape and the arrangement of secondary fibres. Four new species of the genus Euryspongia are differentiated by the fibre structure, cored detritus, shape of sponge, and fibre arrangement. Euryspongia radicula n. sp. is very different from other species by having regularly arranged fibres. Euryspongia spina n. sp. has a fence-like skeletal structure. Bridged type secondary fibres are arranged near the surface and web types are at the base of fibres. Euryspongia flabellum n. sp. has a very unique wide, thin leaf-like shape. String-like primary fibres of E. linea n. sp. are very unique and cored with large sized sands.

• Korean Journal of Acupuncture
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• 제20권4호
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• pp.65-75
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• 2003

This study was carried to identify the component of Spleen Meridian Muscle in human, dividing into outer, middle, and inner part. Lower extremity and trunk were opened widely to demonstrate muscles, nerve, blood vessels and the others, displaying the inner structure of Spleen Meridian Muscle. We obtained the results as follows; 1. Spleen Meridian Muscle is composed of the muscle, nerve and blood vessels. 2. In human anatomy, it is present the difference between a term of nerve or blood vessels which control the muscle of Meridian Muscle and those which pass near by Meridian Muscle. 3. The inner composition of meridian muscle in human arm is as follows ; 1) Muscle; ext. hallucis longus tend., flex. hallucis longus tend.(Sp-1), abd. hallucis tend., flex. hallucis brevis tend., flex. hallucis longus tend.(Sp-2, 3), ant. tibial m. tend., abd. hallucis, flex. hallucis longus tend.(Sp-4), flex. retinaculum, ant. tibiotalar lig.(Sp-5), flex. digitorum longus m., tibialis post. m.(Sp-6), soleus m., flex. digitorum longus m., tibialis post. m.(Sp-7, 8), gastrocnemius m., soleus m.(Sp-9), vastus medialis m.(Sp-10), sartorius m., vastus medialis m., add. longus m.(Sp-11), inguinal lig., iliopsoas m.(Sp-12), ext. abdominal oblique m. aponeurosis, int. abd. ob. m., transversus abd. m.(Sp-13, 14, 15, 16), ant. serratus m., intercostalis m.(Sp-17), pectoralis major m., pectoralis minor m., intercostalis m.(Sp-18, 19, 20), ant. serratus m., intercostalis m.(Sp-21) 2) Nerve; deep peroneal n. br.(Sp-1), med. plantar br. of post. tibial n.(Sp-2, 3, 4), saphenous n., deep peroneal n. br.(Sp-5), sural cutan. n., tibial. n.(Sp-6, 7, 8), tibial. n.(Sp-9), saphenous br. of femoral n.(Sp-10, 11), femoral n.(Sp-12), subcostal n. cut. br., iliohypogastric n., genitofemoral. n.(Sp-13), 11th. intercostal n. and its cut. br.(Sp-14), 10th. intercostal n. and its cut. br.(Sp-15), long thoracic n. br., 8th. intercostal n. and its cut. br.(Sp-16), long thoracic n. br., 5th. intercostal n. and its cut. br.(Sp-17), long thoracic n. br., 4th. intercostal n. and its cut. br.(Sp-18), long thoracic n. br., 3th. intercostal n. and its cut. br.(Sp-19), long thoracic n. br., 2th. intercostal n. and its cut. br.(Sp-20), long thoracic n. br., 6th. intercostal n. and its cut. br.(Sp-21) 3) Blood vessels; digital a. br. of dorsalis pedis a., post. tibial a. br.(Sp-1), med. plantar br. of post. tibial a.(Sp-2, 3, 4), saphenous vein, Ant. Med. malleolar a.(Sp-5), small saphenous v. br., post. tibial a.(Sp-6, 7), small saphenous v. br., post. tibial a., peroneal a.(Sp-8), post. tibial a.(Sp-9), long saphenose v. br., saphenous br. of femoral a.(Sp-10), deep femoral a. br.(Sp-11), femoral a.(Sp-12), supf. thoracoepigastric v., musculophrenic a.(Sp-16), thoracoepigastric v., lat. thoracic a. and v., 5th epigastric v., deep circumflex iliac a.(Sp-13, 14), supf. epigastric v., subcostal a., lumbar a.(Sp-15), intercostal a. v.(Sp-17), lat. thoracic a. and v., 4th intercostal a. v.(Sp-18), lat. thoracic a. and v., 3th intercostal a. v., axillary v. br.(Sp-19), lat. thoracic a. and v., 2th intercostal a. v., axillary v. br.(Sp-20), thoracoepigastric v., subscapular a. br., 6th intercostal a. v.(Sp-21)

• 한국환경과학회지
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• 제24권9호
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• pp.1181-1188
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• 2015

In this study, we discussed about the application of the single physical and chemical treatment processes and the physical-chemical complex treatment processes on the inactivation of Artemia sp. in order to satisfy the USCG Phase II (United States Coast Guard). The results showed that initial disinfection rate of ultrasonic process in single batch process is higher than that of electrolysis. However, the inactivation rate showed slower than electrolysis. The inactivation rate of Artemia sp. on the single continuous treatment process ranked in the following order: homogenizer > electrolysis > ultrasonic process. Inactivation rate of Artemia sp. in continuous homogenizer-electrolysis complex process was reached at 100% immediately. A synergistic effect of ultrasonic-electrolytic complex process was found to be a small. The order of processes in a complex process did not affect the disinfection performance.

• 생명과학회지
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• 제9권6호
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• pp.653-658
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• 1999

A novel marine microorganism, Vibrio sp. YE-101, was isolated from pufferfish and investigated for its ability to synthesize tetrodotoxin (TTX). Various strains isolated from the intestine of pufferfish were grown on TCBS agar plate, and then cultured on Ocean Research Institute (ORI) medium supplemented with 3% NaCl at 23$^{\circ}C$ for 3days. The cells were harvested, disrupted, fractionated by Bio-Gel P-2 column chromatography and then TTX-producing strain, Vibrio sp. YE-101, was identified using mouse bioassay. The isolated TTX from Vibrio sp. YE-101 was also analyzed and identified by HPLC and gas chromatography-mass spectrometer (GC-MS). The mass fragmentation of trimethylsilyl derivatives of C9-base of TTX from Vibrio sp. YE-101 was interpreted and the pattern of fragmentation was same with that of authentic standard. The purfied TTX was also positive to the mouse bioassay, which clearly represents that Vibrio sp. YE-101 can synthesize TTX.

• 한국해양바이오학회지
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• 제9권2호
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• pp.58-64
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• 2017

Polysaccharides are useful materials for keep skin moisturizing and skin improvement cosmetic materials. We have isolated polysaccharides form Gracilariopsis sp. The polysaccharide derived from Gracilariopsis sp. have various size of 300kDa, 500kDa and over 1,000kDa. The volunteers applied a cream formula with polysaccharides from Graciariopsis sp. for 4 weeks. We found significant improvement of wrinkles and skin texture. We also found an effect of improving skin elasticity. When the moisture reduction rate of the skin was 2.8% lower than the 5.1% of hyaluronic acid. The polysaccharide extract from Gracilariopsis sp. showed stable pH and viscosity. The polysaccharides derived from Gracilariopsis sp. could be used as a cosmetic material.

• 한국수산과학회지
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• 제30권3호
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• pp.319-328
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• 1997

연안해역에서 호기성 광합성 세균 Erythrobacter sp. $S\;\pi-I$I를 분리 및 동정하여 rotifer, Brachionus plicatilis의 먹이로 투여하였을 때 개체수, 체장, 체중 및 지방산 조성과 아미노산 조성을 분석한 결과를 요약하면 아래와 같다. 1. Erythrobacter sp. $S\;\pi-I$ 은 지방산의 함량중 $C_{18:1\omega9}$가 $36.5\%,\;C_{18:1\omega7}$이 $13.3\%\;C_{18:3\omega3}\;8.6\%$ 함유하였고 아미노산은 glutamic acid가 $61.6\;mg\%$, aspartic acid가 $45.2\;mg\%$, alanine이 $43.7\;mg\%$, lysine이 $28.7\;mg\%$ 등 높은 함량을 보였다. 2. Erythrobacter sp. $S\;\pi-I$을 rotifer, Brachionus plicatilis에 투여했을 때 (초기 개체수 5개체/$m\ell$) 개체수는 5일째 9개체/$m\ell$, 9일째 20개체/$m\ell$, 13일째는 43개체/$m\ell$로 증가하였다. 또한 체중은 초기 140ng/개체에서 5일째 280ng/개체, 9일째 560ng/개체, 13일째 610ng/개체로 증가하였으며, 체장은 초기 $150{\pm}7{\mu}m\;(length),\;85{\pm}14{\mu}m\;(width)$에서 5일째 $160{\pm}18{\mu}m\;(length),\;92{\pm}15{\mu}m\;(width)$, 9일째 $170{\pm}17{\mu}m\;(length),\;94{\pm}14{\mu}m\;(width)$, 13일째는 $184{\pm}17{\mu}m\;(length),\;100{\pm}12{\mu}m\;(width)$로 성장하였다. 3. Rotifer 개체당 24시간내에 $10^4\~10^5$개체의 세균을 섭취하였다. 4. Erythrobacter sp. $S\;\pi-I$을 rotifer에 먹이로 투여했을때 EPA, DHA가 증가하였다. 비교실험에 사용한 Chlorella sp. PSB, yeast에 비하여 EPA와 DHA의 함량이 높았다. 특히DBHA는 $S\;\pi-I$과 Chlorella sp.에서만 분석되었으며 $S\;\pi-I$이 높았다. 5. Erythrobacter sp. $S\;\pi-I$과 비교로서 PSB, Chlorella sp., yeast를 rotifer에 먹이로 투여했을 때 $S\;\pi-I$에서 lysine이 가장 높았고, PSB, Chlorellaal sp. 순으로 분석되었다. yeast에서는 검출되지 않았다.

• 한국환경농학회지
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• 제19권1호
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• pp.13-19
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• 2000

낙동강 원수에 대한 정수처리과정의 주요 공정인 응집과 여과에 미치는 조류의 장애를 조사하기 위하여 낙동강 하류지역인 칠서정수장 취수원에서 1995년 1월부터 1998년12월까지 발생되는 계절별, 종별 조류 천이과정과 조류가 응집과 여과에 미치는 영향을 실제 정수장에서 적용 사례를 중심으로 조사한 결과는 다음과 같다. 취수원인 낙동강의 Chlorophyll-a 농도는 최저 $20.7{\mu}g/l$ 에서 최고 $180.9{\mu}g/l$ 이었고,총질소와 총인의 농도는 각각 3.4mg/l 와 0.1mg/l 이상으로 년중 과영양상태로 평가되었다. 조류 우점종은 $Stepanodiscus\;sp.\;{\to}\;Asterionella\;sp.\;{\to}\;Melosira\;sp.\;{\to}\;Microcystis\;sp.\;{\to}\;Synedra\;sp.\;{\to}\;Stepanodiscus\;sp.$로 천이되었다. 정수장애는 여과시 폐쇄 및 응집, 침전불량 이었고, 장애 발생빈도는 Stepanodiscus sp.와 Synedra sp.가 각각 42%와 38%이었다. 조류 개체수가 Stepanodiscus sp.와 Synedra sp.는 각각 1,820cells/ml와 800cells/ml일 때 정수장애가 시작되었다. Chlorophyll-a 농도가 $18.9{\mu}g/l$ 일 때 Alum과 PACS의 최적응집제 주입량은 각각 40mg/l 과 16mg/l 에서 처리수 탁도가 가장 양호하였으며, Chlorophyll-a 농도가 $154.1{\mu}g/l$ 로 증가될 때 Alum과 PACS의 최적응집제 주입량은 각각 75mg/l 과 35mg/l 에서 처리수 탁도가 양호하여 Chlorophyll-a 농도가 증가할수록 응집제 주입량이 증가하였다. 응집제를 최적으로 주입하였을때의 조류 제거율은 Stepanodiscus sp. 경우 Alum과 PACS 주입량이 각각 60mg/l 과 30mg/l 일 때 각각 85%와 83%이었으며, Synedra sp. 경우 Alum과 PACS 주입량이 각각 50mg/l 과 25mg/l 일 때 각각 79%와 81%이었다. 여과지에 유입된 Synedra sp.의 개체수가 1,500cells/ml일 때 여과지 폐쇄를 유발하였고, 이 때 여과지속시간은 8hr이었다. 여과지 폐쇄를 일으키는 대부분의 조류는 안트라사이트 표층으로부터 10cm 깊이에서 억류되었으며, Synedra sp.는 표층 5cm 깊이에서 97% 억류되었다.