• Parasites, Hosts and Diseases
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• v.62 no.3
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• pp.281-293
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• 2024

We previously reported that leukotriene B₄ (LTB₄) contained in Trichomonas vaginalis-derived secretory products (TvSP) play an essential role in interleukin-8 (IL-8) production in human mast cell line (HMC-1 cells) via LTB₄ receptor (BLT)-mediated Nuclear Factor-kappa B (NF-κB) activation. Dynamin, a GTPase, has been known to be involved in endocytosis of receptors for signaling of production of cytokine or chemokines. In the present study, we investigated the role of dynamin-mediated BLT1 endocytosis in TvSP-induced IL-8 production. When HMC-1 cells were transfected with BLT1 or BLT2 siRNA, TvSP-induced IL-8 production was significantly inhibited compared with that in cells transfected with control siRNA. In addition, pretreatment of HMC-1 cells with a dynamin inhibitor (Dynasore) reduced IL-8 production induced by TvSP or LTB₄. TvSP- or LTB₄-induced phosphorylation of NF-κB was also attenuated by pretreatment with Dynasore. After exposing HMC-1 cells to TvSP or LTB₄, BLT1 was translocated from the intracellular compartments to the plasma membrane within 30 min. At 60 min after stimulation with TvSP or LTB₄, BLT1 remigrated from the cell surface to intracellular areas. Pretreatment of HMC-1 cells with dynamin-2 siRNA blocked internalization of BLT1 induced by TvSP or LTB₄. Co-immunoprecipitation experiments revealed that dynamin-2 strongly interacted with BLT1 60 min after stimulation with TvSP or LTB₄. These results suggest that T. vaginalis-secreted LTB₄ induces IL-8 production in HMC-1 cells via dynamin 2-mediated endocytosis of BLT1 and phosphorylation of NF-κB.

• The Journal of Internal Korean Medicine
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• v.21 no.1
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• pp.7-12
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• 2000

Objectives : This studies were performed to investigate the correlation between the treatment effects and the changes of the thermal differences at the pre and post-treatment by using the digital infrared computer thermographic imaging (D.I.T.I) to the irritable colon syndrome 30 patients. Methods : The extracts of the gwakhyangjunggisan were administerd to the patients by oral method three times per day during four weeks. we selected the Chung-Wan($CV_{12}$), Chunchu($S_{25}$). Hapkok($LI_4$), Naegwan($P_6$), Yongdo($H_4$) acupoints and treated the patients two times per week during four weeks. One week later, we investigated the changes of the pressure pain around left side Pokkyol($SP_{14}$), the symptoms. and the skin temperature changes on Pokkyol($SP_{14}$) and Kimun($LR_{14}$) around, by using the D.I.T.I. Results : In the correlation between the severity of the pressure pain around left side Pokkyol($SP_{14}$) and the thermal differences on Pokkyol($SP_{14}$) and Kimun($LR_{14}$) around, before the treatment, the more the pain was severe, the more the skin temperature was differential significantly. In the correlation between the severity of the symptoms and the thermal differences on Pokkyol($SP_{14}$) and Kimun($LR_{14}$) around, before the treatment, the more the symptom was severe, the more the skin temperature was differential significantly. In the changes of the pressure pain around left side of Pokkyol($SP_{14}$), the improved were significantly higher than the unimproved. In the changes of the symptoms, the improved were significantly higher than the unimproved. In the correlation between the changes of the pressure pain around left side Pokkyol($SP_{14}$) and the changes of the thermal differences at the pre and post- treatment, the more the pain was improved, the more the thermal change was widened significantly. In the correlation between the changes of the symptoms and the changes of thermal differences at the pre and post-treatment, the more the symptom was improved. the more the thermal change was widened significantly. Conclusions : Above the results, the treatments was effective and the digital infrared computer thermographic imaging (D.I.T.I) was useful to prove the improvements of the pressure pain and the symptom with the irritable bowel syndrome patients.

• Journal of KIISE:Computing Practices and Letters
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• v.5 no.2
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• pp.196-208
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• 1999

본 논문에서는 시뮬레이션 속도 향상을 위하여 VHDL(Very high speed integrated circuit Hardware Description Language)로 기술된 디지털 회로 시뮬레이션을 위한 병렬 분산 VHDL 시뮬레이터(Parallel Distributed VHDL Simulator : PDVS)를 개발한다. 개발된 프로그램을 대규모 병렬 프로그래밍 환경에서도 수행될 수 있도록 하기 위해서 표준 통신 라이브러리인 MPI(Message Passing Interface)를 이용하여 구현된다. PDVS 의 전체적인 시스템구성도, PDVS 에 사용된 시뮬레이션 프로토콜, 전역가상시간 계산 메카니즘 및 논리적 프로세스의 내부 구성요소들간의 관계와 PDVS의 제어 흐름도를 제시한다. 그리고 본 연구에서는 병렬 분산 시뮬레이션의 병렬성 정도를 분석하기 위하여 디지털 회로의 크기 변화와 처리되는 사건수(grain size)의 변화에 따른 성능 결과를 제시한다. 이 연구에서 4배크기의 디지털 회로를 적용한 경우는 프로세서를 12개 사용할 때에 8배의 속도향상을 얻었다. 그리고 처리되는 사건의 수가 200인 경우는 프로세서를 32개 사용할 때에 12배의 속도향상을 얻었다. 또한 동일한 방법을 SGI Origin 2000, Cray T3e 및 IBM SP2에 적용함으로서 그 성능의 간접적인 비교결과도 제시한다.

• Journal of Microbiology and Biotechnology
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• v.31 no.9
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• pp.1210-1217
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• 2021

Two gram-negative, catalase-positive, strictly aerobic, and white colony-forming bacteria, strains H242^T and B156^T, were isolated from soil in South Korea. Cells of strain H242^T were oxidase-positive and non-motile short rods, while those of strain B156^T were oxidase-negative and long non-motile rods. Ubiquinone-8 was identified as the sole isoprenoid quinone in both strains. C_16:0, cyclo-C_17:0, andsummed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c) and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were identified in both strains as the major cellular fatty acids and polar lipids, respectively. The DNA G+C contents of strains H242^T and B156^T were 69.4 mol% and 69.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA and 92 concatenated core gene sequences revealed that strains H242^T and B156^T formed distinct phylogenic lineages from other Ramlibacter type strains. The DNA-DNA hybridization (DDH) value between strains H242^T and B156^T was 24.6%. Strains H242^T and B156^T were most closely related to Ramlibacter ginsenosidimutans BXN5-27^T and Ramlibacter monticola G-3-2^T with 98.4% and 98.6% 16S rRNA gene sequence similarities, respectively. Digital DDH values between strain H242^T and R. ginsenosidimutans and between strain B156^T and R. monticola were 23.5% and 26.1%, respectively. Phenotypic, chemotaxonomic, and molecular analyses indicated that strains H242^T and B156^T represent two novel species of the genus Ramlibacter, for which the names Ramlibacter terrae sp. nov. and Ramlibacter montanisoli sp. nov., respectively, are proposed. The type strains of R. terrae and R. montanisoli are H242^T (=KACC 21667^T=JCM 33922^T) and B156^T (=KACC 21665^T=JCM 33920^T), respectively.

• Korean Journal Plant Pathology
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• v.11 no.4
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• pp.298-303
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• 1995

The biological control effect of Trichoderma harzianum on the Fusarium wilt of strawberry and several factors affecting on its efficacy were examined through pot experiments. T. harzianum grown on wheat barn, rice straw, rice hull, sawdust or barley straw was respectively incorporated into the pathogen-infected soil, and significantly suppressed the strawberry wilt caused by Fusarium oxysporum f. sp. fragariae. The wheat bran or rice straw culture of T. harzianum suppressed the disease incidence more effectively than other substrates for culture, decreasing it to 68% of the untreated control. The conidial suspension of T. harzianum alone or the suspension mixed with crab shell also effectively reduced the disease incidence. The control effectiveness of T. harzianum was high in acid soil (pH 3.5~5.5). In sandy loam soil, the disease incidences and population densities of the pathogen were decreased by the treatment of T. harzianum, while there was no significant effect of T. harzianum on the pathogen in loam soil.

• Journal of Life Science
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• v.8 no.2
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• pp.173-180
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• 1998

As a part of the investigation on useful compounds from microalgae, and its recently that marine planktonic algae have been recognized as potial sources of antibacterial and antifungal substances, we searched for antimicrobial active compounds from the extracts of six microalgae-Lyngbya sp., Tetraselmis sp., Microcystis sp., Chlorella sp., Navicula sp. and Rhalassiosira sp.-treated with several solvents. There were two active species-Lyngbya ap., Teraselmis sp.- in the antimicrobial activity test to bacteria, yeast and molds, especially the activity existed in the extracts by ethyl acetate of supernatants to the microalgae incubatio. and there won’t any activity in two diatoms to the test microorganism.

• Journal of Microbiology and Biotechnology
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• v.16 no.12
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• pp.1995-1999
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• 2006

Pseudomonas sp. K82 cultured in p-hydroxybenzoate induces protocatechuate 4,5-dioxygenase (PCD 4,5) for p-hydroxybenzoate degradation. In this study, a 6.0-kbp EcoR1 fragment containing p-hydroxybenzoate degradation genes was cloned from the genome of Pseudomonas sp. K82. Sequence analysis identified four genes, namely, pcaD, pcaA, pcaB, and pcaC genes known to be involved in p-hydroxybenzoate degradation. Two putative 4-hydroxyphenylpyruvate dioxygenases and one putative oxidoreductase were closely located by the p-hydroxybenzoate degradation genes. The gene arrangement and sequences of these p-hydroxybenzoate degradation genes were similar to those of Comamonas testosteroni and Pseudomonas ochraceae. PcaAB (PCD4,5) was overexpressed in the expression vector pGEX-4T-3, purified using a GST column, and confirmed to have protocatechuate 4,5-dioxygenase activity. The N-terminal amino acid sequences of overexpressed PCD4,5 were identical with those of purified PCD4,5 from Pseudomonas sp. K82.

• Microbiology and Biotechnology Letters
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• v.22 no.4
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• pp.423-429
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• 1994

A bacterial strain which formed a distinct colony on agar plate containing naphthalene as a vapor phase and grew well ina liquid minimal medium was isolated and identified as Alcaligenes sp. A111. Optimum temperature and pH for the cultivation of Alcaligenes sp. A111 were 30$\cir$C and 7.0, respectively. Cell growth increased dramatically from 12 hours after inoculation and revealed a stationary phase at about 48 hours. Relative growth rate ($\mu$')increased hyperbolically depending on the conceration of naphthalene up to 500 ppm and reached to the maximum value pf 2.8/day, but $\mu$' didn't change within a range of 500~4000 ppm naphthalene. NH$_{4}$Cl or NH$_{4}$NO$_{3}$ was preferrd as a nitrogen source and a P : N ratio by weight og 6 : 1 was favorable to cell growth. Alcaligenes sp. A111 utilized the intermediates of degradation of naphthalene and showed tolerance to benzene, toluene, and octane. therefore, it is suggested that Alcaligenes sp. A111 could be effectively used for the biological treatment of wastewater containing naphthalene in the presence of some aromatic compounds.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.3
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• pp.359-367
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• 1999

For investigating the activities of algicidal bacteria, the variations of algicidal bacterial population and chlorophyll-a were checked weekly in Chindong Bay, Korea from May to July, 1998. For identifying their killing characteristics, three strains were selected from the isolated algicidal bacteria. The density of algicidal bacteria kept changing in the range of $6.0\times10^1$ to $6.4\times10^5$ cell $\ell^{-1}$. The density flux of algicidal bacteria coincided with that of chlorophyll-a by a week of lag time. Three algicidal bacteria isolated from field strains, H519S5-4, H605S5-15 and H605S5-22, were investigated in nine microalgal species, Heterosigma akashiwo, Chattonella sp. (Raphidlphyceae), Gymnodinium catenatum, Gyrodinium impudicum, Cochlodinium polyklikoides (Dinophyceae), Chaetoceros sp., Coscinodiscus granii, Ditylum brightwellii, Thalassiosira rotula (Bacillariophyceae). Strain H605S5-22 showed a wide algicidal activities over nine microlgae, strain H605S5-15 over H. akashiwo, G. catenatum, T. rotula, Chattonella sp. and strain H519S5-4 over H. akashiwo, Chattonella sp., Chaetoceros sp., G. catenatum. The activities of the three strains were detected by the secretion of algicidal substances. Therefore, it is suggested that the activities of algicidal bacteria have a significant influence over the population dynamics of phytoplankton and get involved with the sharp decrease in red tides in the coastal area.

• Journal of Microbiology and Biotechnology
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• v.32 no.5
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• pp.575-581
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• 2022

A Gram-stain-negative, white-coloured, and rod-shaped bacterium, strain DR4-4^T, was isolated from Daechung Reservoir, Republic of Korea, during Microcystis bloom. Strain DR4-4^T was most closely related to Caenimonas terrae SGM1-15^T and Caenimonas koreensis EMB320^T with 98.1% 16S rRNA gene sequence similarities. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain DR4-4^T and closely related type strains were below 79.46% and 22.30%, respectively. The genomic DNA G+C content was 67.5%. The major cellular fatty acids (≥10% of the total) were identified as C_16:0, cyclo C_17:0, summed feature 3 (C_16:1ω7c and/or C_16:1ω6c), and summed feature 8 (C_18:1ω7c and/or C_18:1ω6c). Strain DR4-4^T possessed phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol as the main polar lipids and Q-8 as the respiratory quinone. The polyamine profile was composed of putrescine, cadaverine, and spermidine. The results of polyphasic characterization indicated that the isolated strain DR4-4^T represents a novel species within the genus Caenimonas, for which the name Caenimonas aquaedulcis sp. nov. is proposed. The type strain is DR4-4^T (=KCTC 82470^T =JCM 34453^T).